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The map label for this gene is yfkN [H]

Identifier: 108562529

GI number: 108562529

Start: 108461

End: 110206

Strand: Reverse

Name: yfkN [H]

Synonym: HPAG1_0104

Alternate gene names: 108562529

Gene position: 110206-108461 (Counterclockwise)

Preceding gene: 108562530

Following gene: 108562528

Centisome position: 6.9

GC content: 39.75

Gene sequence:

>1746_bases
ATGAGAAAGTTGATTTTATTCATCTTTTTAACGCTAACACTTTCAGTATCTGCAAAAGAAGTGAAAATCGTGTTTTTAGA
AACTTCAGACATTCATGGGCGGCTTTTTTCGTATGATTATGCGATTGGCGAACAAAAACCCAATAACGGCTTGACAAGGA
TTGCGACTTTAATCAAAAAACAGAGGGCTGAGAATAAAAATGTGGTTTTGATTGACAGCGGGGATTTGTTACAAGGTAAT
AGCGCGGAGTTGTTTAACGATGAGCCTATCCACCCGCTAGTTAGAGCTGAAAACGATTTGAAATTTGATGTTCGTGTGCT
TGGCAATCACGAGTTTAACTTCAGTAAAGATTTTTTAGAGAAAAATATTAAGGGGTTTAATGGCGGTGTCGTGAATGCGA
ATATTATCAAAATTGCGGACAATAAGCCGTTTGTGAAGCCTTATATGATTAAAAAAATTGATGGCGTGAGGGTGGCGGTT
GTGGGGTTTTTGGTGCCGCATGTCCCCACTTGGGAAGCCTCTACGCCTGAACATTTTGCAGGATTGAAGTTTTTGGACGC
TGAAGAAGCGTTAAAAAAGACCTTGAAAGAGTTAAAAGGGAAGTATGATATTTTGATTGGCGCTTTTCATTTGGGGCGAG
AAGATGAGAAAGGTGGCGACGGGATACCTGATCTGGCGAAAAAATTCCCGCAATTTGACATCATTTTTGCAGGGCATGAG
CATGCGGTTTATAACACTAAAATAGGAAAGGTGCATACCATTGAGCCTGGAGCGTATGGGGCTTATCTAGCCAAAGGCGT
GGTAATATTTGACACCCAAACGAAGAAAAAAACCATAACGACTGAAAATTTACCCACAAAAGGCGTGCCAGAAGACGAAG
AATTGGCGAAAAAATATAAATATGTGGATAAAAAATCAAAAGAACACGCTAATGAAGTGGTCGGCGAAGTGACAAAAACC
TTTATTGACAGGCCTGATTTTATCACAGGAGGAGAAAAAATCACCACGATGCCCACCGCCGCCTTGCAAGAAACACCGGT
GATAGAACTGATTAATAAAGTGCAAAAATATTATGCAAAAGCCGATGTTTCAGCGGCAGCCTTATTCAATTTTGGGGCGA
ATTTGAAAAAAGGGCCTTTCAAAAGAAAAGATGTCGCCTATATTTACAAGTTCGCTAACACGCTCATTGGAGTGAAAATA
ACGGGTGAAAACCTGTTGAAATACATGGAATGGTCATACCGATTTTACAACCAGTTGCAACCAGGAGATTTGACGATCAG
TTTTAATGAAAACATTCGCGGCTATAACTTTGACATGTTTTCTGGGGTGAAATACCAGGTTGATGTTACCAAACCCGCCG
GACAAAGGATTATCAATCCGACCATCAACAACAAACCCATTGACCCCAAAGCGATCTATAAATTAGCGATCAACAATTAC
CGATTCGGGACATTATCCACGACATTAAAATTGGTTACAGACGCCAATAGGTATTATAATTCTTACGATGAACTGCAAGA
TAATGGGCAAATACGGGATTTAATCATCAAATACATCACTGAAGAAAAAGGTGGGAAGGTAACCCCTGAACTGGAACATA
ACTGGGAAATCATCCACTACGATTTCAAAAACCCGTTGTTGGAAAAATTGAGAGAAAAATTAAAAGAGGGGAGCATCAAA
ATCCCCACTTCAAAAGATGGGAGGACTTTGAATATCAAATCCATTAAAGAGAGTGAAGTGAAATAA

Upstream 100 bases:

>100_bases
AATCTTACCCATAGGCGTTAAATTCTAGCAGGAATCATCGCATCAGCGTTAATATTATGTAAAAATAATGTTATGAAGAT
TTTTTTATGGAAGGATTTCA

Downstream 100 bases:

>100_bases
AATTTTTTATTTTTATTATTTTATCTTTAAGCCTAACTTAAAAAAGGGTGTTCTATAATTTTAGCTTGTTTGTTCTTTTG
TTTCGTTTTCAAACAACCGG

Product: 2',3'-cyclic-nucleotide 2'-phosphodiesterase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MRKLILFIFLTLTLSVSAKEVKIVFLETSDIHGRLFSYDYAIGEQKPNNGLTRIATLIKKQRAENKNVVLIDSGDLLQGN
SAELFNDEPIHPLVRAENDLKFDVRVLGNHEFNFSKDFLEKNIKGFNGGVVNANIIKIADNKPFVKPYMIKKIDGVRVAV
VGFLVPHVPTWEASTPEHFAGLKFLDAEEALKKTLKELKGKYDILIGAFHLGREDEKGGDGIPDLAKKFPQFDIIFAGHE
HAVYNTKIGKVHTIEPGAYGAYLAKGVVIFDTQTKKKTITTENLPTKGVPEDEELAKKYKYVDKKSKEHANEVVGEVTKT
FIDRPDFITGGEKITTMPTAALQETPVIELINKVQKYYAKADVSAAALFNFGANLKKGPFKRKDVAYIYKFANTLIGVKI
TGENLLKYMEWSYRFYNQLQPGDLTISFNENIRGYNFDMFSGVKYQVDVTKPAGQRIINPTINNKPIDPKAIYKLAINNY
RFGTLSTTLKLVTDANRYYNSYDELQDNGQIRDLIIKYITEEKGGKVTPELEHNWEIIHYDFKNPLLEKLREKLKEGSIK
IPTSKDGRTLNIKSIKESEVK

Sequences:

>Translated_581_residues
MRKLILFIFLTLTLSVSAKEVKIVFLETSDIHGRLFSYDYAIGEQKPNNGLTRIATLIKKQRAENKNVVLIDSGDLLQGN
SAELFNDEPIHPLVRAENDLKFDVRVLGNHEFNFSKDFLEKNIKGFNGGVVNANIIKIADNKPFVKPYMIKKIDGVRVAV
VGFLVPHVPTWEASTPEHFAGLKFLDAEEALKKTLKELKGKYDILIGAFHLGREDEKGGDGIPDLAKKFPQFDIIFAGHE
HAVYNTKIGKVHTIEPGAYGAYLAKGVVIFDTQTKKKTITTENLPTKGVPEDEELAKKYKYVDKKSKEHANEVVGEVTKT
FIDRPDFITGGEKITTMPTAALQETPVIELINKVQKYYAKADVSAAALFNFGANLKKGPFKRKDVAYIYKFANTLIGVKI
TGENLLKYMEWSYRFYNQLQPGDLTISFNENIRGYNFDMFSGVKYQVDVTKPAGQRIINPTINNKPIDPKAIYKLAINNY
RFGTLSTTLKLVTDANRYYNSYDELQDNGQIRDLIIKYITEEKGGKVTPELEHNWEIIHYDFKNPLLEKLREKLKEGSIK
IPTSKDGRTLNIKSIKESEVK
>Mature_581_residues
MRKLILFIFLTLTLSVSAKEVKIVFLETSDIHGRLFSYDYAIGEQKPNNGLTRIATLIKKQRAENKNVVLIDSGDLLQGN
SAELFNDEPIHPLVRAENDLKFDVRVLGNHEFNFSKDFLEKNIKGFNGGVVNANIIKIADNKPFVKPYMIKKIDGVRVAV
VGFLVPHVPTWEASTPEHFAGLKFLDAEEALKKTLKELKGKYDILIGAFHLGREDEKGGDGIPDLAKKFPQFDIIFAGHE
HAVYNTKIGKVHTIEPGAYGAYLAKGVVIFDTQTKKKTITTENLPTKGVPEDEELAKKYKYVDKKSKEHANEVVGEVTKT
FIDRPDFITGGEKITTMPTAALQETPVIELINKVQKYYAKADVSAAALFNFGANLKKGPFKRKDVAYIYKFANTLIGVKI
TGENLLKYMEWSYRFYNQLQPGDLTISFNENIRGYNFDMFSGVKYQVDVTKPAGQRIINPTINNKPIDPKAIYKLAINNY
RFGTLSTTLKLVTDANRYYNSYDELQDNGQIRDLIIKYITEEKGGKVTPELEHNWEIIHYDFKNPLLEKLREKLKEGSIK
IPTSKDGRTLNIKSIKESEVK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=620, Percent_Identity=28.2258064516129, Blast_Score=209, Evalue=6e-55,
Organism=Escherichia coli, GI1786687, Length=255, Percent_Identity=27.4509803921569, Blast_Score=79, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24641187, Length=503, Percent_Identity=20.8747514910537, Blast_Score=71, Evalue=2e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 65760; Mature: 65760

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLILFIFLTLTLSVSAKEVKIVFLETSDIHGRLFSYDYAIGEQKPNNGLTRIATLIKK
CHHHHEEHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
QRAENKNVVLIDSGDLLQGNSAELFNDEPIHPLVRAENDLKFDVRVLGNHEFNFSKDFLE
HHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCEEEEEEECCCCCCCCHHHHH
KNIKGFNGGVVNANIIKIADNKPFVKPYMIKKIDGVRVAVVGFLVPHVPTWEASTPEHFA
HHCCCCCCCEEEEEEEEEECCCCCCCCHHHHHCCCCEEEEEHHHHCCCCCCCCCCCHHHC
GLKFLDAEEALKKTLKELKGKYDILIGAFHLGREDEKGGDGIPDLAKKFPQFDIIFAGHE
CCEEECHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHCCCEEEEEECCC
HAVYNTKIGKVHTIEPGAYGAYLAKGVVIFDTQTKKKTITTENLPTKGVPEDEELAKKYK
CEEEECCCCEEEEECCCCCHHHHHCCEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHH
YVDKKSKEHANEVVGEVTKTFIDRPDFITGGEKITTMPTAALQETPVIELINKVQKYYAK
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEECCHHHHCCCCHHHHHHHHHHHHHH
ADVSAAALFNFGANLKKGPFKRKDVAYIYKFANTLIGVKITGENLLKYMEWSYRFYNQLQ
CCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHCCCC
PGDLTISFNENIRGYNFDMFSGVKYQVDVTKPAGQRIINPTINNKPIDPKAIYKLAINNY
CCCEEEEECCCCCCCCEEHCCCEEEEEEECCCCCCEEECCCCCCCCCCHHHEEEEEECCE
RFGTLSTTLKLVTDANRYYNSYDELQDNGQIRDLIIKYITEEKGGKVTPELEHNWEIIHY
EEEEHHHHEEEEECCHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
DFKNPLLEKLREKLKEGSIKIPTSKDGRTLNIKSIKESEVK
ECCCHHHHHHHHHHHCCCEECCCCCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MRKLILFIFLTLTLSVSAKEVKIVFLETSDIHGRLFSYDYAIGEQKPNNGLTRIATLIKK
CHHHHEEHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
QRAENKNVVLIDSGDLLQGNSAELFNDEPIHPLVRAENDLKFDVRVLGNHEFNFSKDFLE
HHCCCCCEEEEECCCCCCCCCCCCCCCCCCCHHHCCCCCCEEEEEEECCCCCCCCHHHHH
KNIKGFNGGVVNANIIKIADNKPFVKPYMIKKIDGVRVAVVGFLVPHVPTWEASTPEHFA
HHCCCCCCCEEEEEEEEEECCCCCCCCHHHHHCCCCEEEEEHHHHCCCCCCCCCCCHHHC
GLKFLDAEEALKKTLKELKGKYDILIGAFHLGREDEKGGDGIPDLAKKFPQFDIIFAGHE
CCEEECHHHHHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCHHHHHHCCCEEEEEECCC
HAVYNTKIGKVHTIEPGAYGAYLAKGVVIFDTQTKKKTITTENLPTKGVPEDEELAKKYK
CEEEECCCCEEEEECCCCCHHHHHCCEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHH
YVDKKSKEHANEVVGEVTKTFIDRPDFITGGEKITTMPTAALQETPVIELINKVQKYYAK
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCEEEECCHHHHCCCCHHHHHHHHHHHHHH
ADVSAAALFNFGANLKKGPFKRKDVAYIYKFANTLIGVKITGENLLKYMEWSYRFYNQLQ
CCCHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHCCCC
PGDLTISFNENIRGYNFDMFSGVKYQVDVTKPAGQRIINPTINNKPIDPKAIYKLAINNY
CCCEEEEECCCCCCCCEEHCCCEEEEEEECCCCCCEEECCCCCCCCCCHHHEEEEEECCE
RFGTLSTTLKLVTDANRYYNSYDELQDNGQIRDLIIKYITEEKGGKVTPELEHNWEIIHY
EEEEHHHHEEEEECCHHHHCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
DFKNPLLEKLREKLKEGSIKIPTSKDGRTLNIKSIKESEVK
ECCCHHHHHHHHHHHCCCEECCCCCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]