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The map label for this gene is hypF [H]

Identifier: 108562469

GI number: 108562469

Start: 46542

End: 48851

Strand: Reverse

Name: hypF [H]

Synonym: HPAG1_0044

Alternate gene names: 108562469

Gene position: 48851-46542 (Counterclockwise)

Preceding gene: 108562470

Following gene: 108562468

Centisome position: 3.06

GC content: 40.78

Gene sequence:

>2310_bases
TTGCAATACCTTAATCAAACAGCATGGAAGCTTGCATTGCGTAACGATGCAACTTTATTGAACAAGATCACGCTTTTTGG
CGTGGTTCAAGGCGTGGGCATGCGCCCTTTTATTTATACCCTAGCTCAAAAATTAGAGCTTGTAGGCTTTGTGCGTAACA
CCCAAGCGGCTTTGGAAATCATCTTGCCCGCTCATCAAACAGAGTCTTTTTTAAACGCTCTCAAAAAAGGGTTACCCCCT
TTAGCGTTGGTTGAAAAAATCACTATTAGCCCTTATGATAAGGCGCTTCATTTCAATGGTTTTAGGATTTTAGAAAGCAA
GAACCACCCTTTAAATTTGCTCAGTCAAATCCCTAAAGATTTAGGCGTGTGCAAGGATTGCTTGCACGAAATTAGAGATA
AAAACTCCCCTTATTTTCATTACGCTTTCAATTCTTGTGCGAAATGCGGGGCGAGATACAGCCTTTTAAACGCCCTACCC
TATGACAGAGAAAACTCCGCCTTAAAACCTTTCAAACTCTGTGAATTTTGTGCTTCTATTTATCAAGACCCTACCAATAA
GCGCTTCCACATTCAAGGCATCAGCTGCAAAAAGTGCGGTATCGTGCTCAATTATAAGCGATTCAAAAACGATGACGCTC
TTTTAGAATGCGCTAAAGACATTCAAAAAGGTAAAATCATCGCTCTTAAAGGTTTGGGAGGCTTTGCTCTCTTGTGCGAT
GCGAGGAATTTTCAAACCATAGAAAGGTTACGGCTTTTAAAAAACCGCCCCCTAAAGCCTTTTGCGCTCATGTTTAAAGA
CTTAAACTCAGTCAAACAGCATGCGTTTTTGAACGCGTTAGAATGCGAAAGTTTGAGTTCGATAAGTGCCCCCATTCTTT
TAGCGCGTAAAAAACCTGACACCCAATTAGCCCCCAATATCGCTAAAAACTCCTCCTTTTATGGCGTTATCTTGCCCTAT
ACCCCTTTGCATGCTCTATTATTGGATTTATTGGATTTCCCTATTGTGTTCACGAGCGCGAATTTCAGCTCCCTCCCTTT
AGCGAACGATGAGGCTGAGATTGATGCCTTGAGTTTCATTTTTGATTTCAAGCTCACACACAATCGCGCTATCATTCACA
GGATTGATGACAGCATCGCACAATGCATAGACAACGCGATTCGCCCCATGCGTTTGGCTAGGGGGTTTGCCCCACTTTAT
CTCACTTTGCCTAAACGCTCTAATGGTTCGCAAAAAAAGATTTTAGCGCTTGGAGCGGAGCAAAAAGGGCATTTTAGCTT
ACTAGATAGTGAAACTTCTACCTTGTTACTCTCGCCTTTTTGTGGGGATTTGAGCGTTTTAGAAAATGAAAAACACTTTA
AAGAAACTTTGAATTTTTTCTTAAAAACCTATGATTTCAAGCCCACGCTCTTAGCTTGCGACAAGCATAAAAACTACACC
ACCACTAAAATGGCTTTTGATTTTAATACGCCCTTATTGCAAGTCCAGCACCACCATGCCCACTTTTTAGCGAGTATCTT
AGACGCATTGTTACAAGATCCGCATTTAAACCACCCTTTTATAGGTATTGTTTGGGATGGGAGTGGGGCTTATGAAAATA
AGATTTATGGGGCGGAGTGTTTTGTGGGGGATTTGGAACGCATTGAAGAGATGGCCAGGTTTGAAGAATTTCTGCTTTTA
GGGGGGCAAAAAGCGATCAAAGAGCCTAAACGCTTGGTTTTAGAAATCGCTTTAAAACACCAACTCAACAAGCTTTTAAA
ACGCGTTCAAAAGCATTTTAAAGAAGACGAATTAGAAATTTTCCAACAAATGCATGACAAAAAAATTCAAAGCATCGCCA
CCAATTCCATAGGGCGTTTGTTTGATATAGTAGCGTTTAGTTTGGATTTAACAGGAACGATTAGTTTTGAAGCCGAGAGT
GGGCAGGTTTTAGAAAATCTAGCCTTACAAAGCGATGAGATCGCCTTTTACCCTTTTACAATCAAAAACAGCGTGGTGTG
TTTAAAAGATTTTTATCAAGCGTTTGAAAAGGATTTGGGCGTTTTAGAACCTAAACATATCGCTAAGAAATTTTTTAACA
GCCTAGTAGAAATCATTACCGCTTTGATTGCGCCTTTTAAAGAGCATGTGGTGGTGTGCAGTGGGGGCGTGTTTTGCAAC
CAATTATTGTGCGAACAATTAGCCAAACGATTGAGGGGGCTAAAAAGGCAGTATTTTTTCCACAAGCATTTCCCCCCTAA
CGATAGCAGTATCCCTATCGGTCAAGCCTTAATGGCGTATTTCAACCCTACAATCATTAAAAAAGGATAA

Upstream 100 bases:

>100_bases
GCAATAACGCTTTAATCGTCCCCACTTACAACGACCCTAAAGACGCGCTCATTTTAGAAACCTTAAAACAACACACGCCC
TTAGAAGTGATAGGGGTTGA

Downstream 100 bases:

>100_bases
AAATGGATAGCGTAACTCTAGCATGCGGGAATGGGGGGAAAGAAACAAACGCTTTAATTGAGCGGATCTTTATGCCCTAT
TTAAAAGAATTTATCGTTGC

Product: transcriptional regulator

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 769; Mature: 769

Protein sequence:

>769_residues
MQYLNQTAWKLALRNDATLLNKITLFGVVQGVGMRPFIYTLAQKLELVGFVRNTQAALEIILPAHQTESFLNALKKGLPP
LALVEKITISPYDKALHFNGFRILESKNHPLNLLSQIPKDLGVCKDCLHEIRDKNSPYFHYAFNSCAKCGARYSLLNALP
YDRENSALKPFKLCEFCASIYQDPTNKRFHIQGISCKKCGIVLNYKRFKNDDALLECAKDIQKGKIIALKGLGGFALLCD
ARNFQTIERLRLLKNRPLKPFALMFKDLNSVKQHAFLNALECESLSSISAPILLARKKPDTQLAPNIAKNSSFYGVILPY
TPLHALLLDLLDFPIVFTSANFSSLPLANDEAEIDALSFIFDFKLTHNRAIIHRIDDSIAQCIDNAIRPMRLARGFAPLY
LTLPKRSNGSQKKILALGAEQKGHFSLLDSETSTLLLSPFCGDLSVLENEKHFKETLNFFLKTYDFKPTLLACDKHKNYT
TTKMAFDFNTPLLQVQHHHAHFLASILDALLQDPHLNHPFIGIVWDGSGAYENKIYGAECFVGDLERIEEMARFEEFLLL
GGQKAIKEPKRLVLEIALKHQLNKLLKRVQKHFKEDELEIFQQMHDKKIQSIATNSIGRLFDIVAFSLDLTGTISFEAES
GQVLENLALQSDEIAFYPFTIKNSVVCLKDFYQAFEKDLGVLEPKHIAKKFFNSLVEIITALIAPFKEHVVVCSGGVFCN
QLLCEQLAKRLRGLKRQYFFHKHFPPNDSSIPIGQALMAYFNPTIIKKG

Sequences:

>Translated_769_residues
MQYLNQTAWKLALRNDATLLNKITLFGVVQGVGMRPFIYTLAQKLELVGFVRNTQAALEIILPAHQTESFLNALKKGLPP
LALVEKITISPYDKALHFNGFRILESKNHPLNLLSQIPKDLGVCKDCLHEIRDKNSPYFHYAFNSCAKCGARYSLLNALP
YDRENSALKPFKLCEFCASIYQDPTNKRFHIQGISCKKCGIVLNYKRFKNDDALLECAKDIQKGKIIALKGLGGFALLCD
ARNFQTIERLRLLKNRPLKPFALMFKDLNSVKQHAFLNALECESLSSISAPILLARKKPDTQLAPNIAKNSSFYGVILPY
TPLHALLLDLLDFPIVFTSANFSSLPLANDEAEIDALSFIFDFKLTHNRAIIHRIDDSIAQCIDNAIRPMRLARGFAPLY
LTLPKRSNGSQKKILALGAEQKGHFSLLDSETSTLLLSPFCGDLSVLENEKHFKETLNFFLKTYDFKPTLLACDKHKNYT
TTKMAFDFNTPLLQVQHHHAHFLASILDALLQDPHLNHPFIGIVWDGSGAYENKIYGAECFVGDLERIEEMARFEEFLLL
GGQKAIKEPKRLVLEIALKHQLNKLLKRVQKHFKEDELEIFQQMHDKKIQSIATNSIGRLFDIVAFSLDLTGTISFEAES
GQVLENLALQSDEIAFYPFTIKNSVVCLKDFYQAFEKDLGVLEPKHIAKKFFNSLVEIITALIAPFKEHVVVCSGGVFCN
QLLCEQLAKRLRGLKRQYFFHKHFPPNDSSIPIGQALMAYFNPTIIKKG
>Mature_769_residues
MQYLNQTAWKLALRNDATLLNKITLFGVVQGVGMRPFIYTLAQKLELVGFVRNTQAALEIILPAHQTESFLNALKKGLPP
LALVEKITISPYDKALHFNGFRILESKNHPLNLLSQIPKDLGVCKDCLHEIRDKNSPYFHYAFNSCAKCGARYSLLNALP
YDRENSALKPFKLCEFCASIYQDPTNKRFHIQGISCKKCGIVLNYKRFKNDDALLECAKDIQKGKIIALKGLGGFALLCD
ARNFQTIERLRLLKNRPLKPFALMFKDLNSVKQHAFLNALECESLSSISAPILLARKKPDTQLAPNIAKNSSFYGVILPY
TPLHALLLDLLDFPIVFTSANFSSLPLANDEAEIDALSFIFDFKLTHNRAIIHRIDDSIAQCIDNAIRPMRLARGFAPLY
LTLPKRSNGSQKKILALGAEQKGHFSLLDSETSTLLLSPFCGDLSVLENEKHFKETLNFFLKTYDFKPTLLACDKHKNYT
TTKMAFDFNTPLLQVQHHHAHFLASILDALLQDPHLNHPFIGIVWDGSGAYENKIYGAECFVGDLERIEEMARFEEFLLL
GGQKAIKEPKRLVLEIALKHQLNKLLKRVQKHFKEDELEIFQQMHDKKIQSIATNSIGRLFDIVAFSLDLTGTISFEAES
GQVLENLALQSDEIAFYPFTIKNSVVCLKDFYQAFEKDLGVLEPKHIAKKFFNSLVEIITALIAPFKEHVVVCSGGVFCN
QLLCEQLAKRLRGLKRQYFFHKHFPPNDSSIPIGQALMAYFNPTIIKKG

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=756, Percent_Identity=32.1428571428571, Blast_Score=301, Evalue=1e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 87012; Mature: 87012

Theoretical pI: Translated: 8.82; Mature: 8.82

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQYLNQTAWKLALRNDATLLNKITLFGVVQGVGMRPFIYTLAQKLELVGFVRNTQAALEI
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHE
ILPAHQTESFLNALKKGLPPLALVEKITISPYDKALHFNGFRILESKNHPLNLLSQIPKD
EECCHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHEEECCEEEEECCCCCHHHHHHCHHH
LGVCKDCLHEIRDKNSPYFHYAFNSCAKCGARYSLLNALPYDRENSALKPFKLCEFCASI
HHHHHHHHHHHHCCCCCEEEEHHHHHHHHCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
YQDPTNKRFHIQGISCKKCGIVLNYKRFKNDDALLECAKDIQKGKIIALKGLGGFALLCD
HCCCCCCEEEEECCCCCCCCEEEEHHHHCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEC
ARNFQTIERLRLLKNRPLKPFALMFKDLNSVKQHAFLNALECESLSSISAPILLARKKPD
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
TQLAPNIAKNSSFYGVILPYTPLHALLLDLLDFPIVFTSANFSSLPLANDEAEIDALSFI
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHH
FDFKLTHNRAIIHRIDDSIAQCIDNAIRPMRLARGFAPLYLTLPKRSNGSQKKILALGAE
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECCC
QKGHFSLLDSETSTLLLSPFCGDLSVLENEKHFKETLNFFLKTYDFKPTLLACDKHKNYT
CCCCEEEECCCCCEEEECCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCE
TTKMAFDFNTPLLQVQHHHAHFLASILDALLQDPHLNHPFIGIVWDGSGAYENKIYGAEC
EEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCHH
FVGDLERIEEMARFEEFLLLGGQKAIKEPKRLVLEIALKHQLNKLLKRVQKHFKEDELEI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FQQMHDKKIQSIATNSIGRLFDIVAFSLDLTGTISFEAESGQVLENLALQSDEIAFYPFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECEEEEEEECCCCHHHHHHHCCCCCEEEEEEE
IKNSVVCLKDFYQAFEKDLGVLEPKHIAKKFFNSLVEIITALIAPFKEHVVVCSGGVFCN
ECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHCEEEECCCHHHH
QLLCEQLAKRLRGLKRQYFFHKHFPPNDSSIPIGQALMAYFNPTIIKKG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECC
>Mature Secondary Structure
MQYLNQTAWKLALRNDATLLNKITLFGVVQGVGMRPFIYTLAQKLELVGFVRNTQAALEI
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCHHHHHHE
ILPAHQTESFLNALKKGLPPLALVEKITISPYDKALHFNGFRILESKNHPLNLLSQIPKD
EECCHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHEEECCEEEEECCCCCHHHHHHCHHH
LGVCKDCLHEIRDKNSPYFHYAFNSCAKCGARYSLLNALPYDRENSALKPFKLCEFCASI
HHHHHHHHHHHHCCCCCEEEEHHHHHHHHCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
YQDPTNKRFHIQGISCKKCGIVLNYKRFKNDDALLECAKDIQKGKIIALKGLGGFALLCD
HCCCCCCEEEEECCCCCCCCEEEEHHHHCCCHHHHHHHHHHCCCCEEEEECCCCEEEEEC
ARNFQTIERLRLLKNRPLKPFALMFKDLNSVKQHAFLNALECESLSSISAPILLARKKPD
CCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCC
TQLAPNIAKNSSFYGVILPYTPLHALLLDLLDFPIVFTSANFSSLPLANDEAEIDALSFI
CCCCCCCCCCCCEEEEECCCCHHHHHHHHHHCCCEEEECCCCCCCCCCCCCHHHHHHHHH
FDFKLTHNRAIIHRIDDSIAQCIDNAIRPMRLARGFAPLYLTLPKRSNGSQKKILALGAE
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEEECCC
QKGHFSLLDSETSTLLLSPFCGDLSVLENEKHFKETLNFFLKTYDFKPTLLACDKHKNYT
CCCCEEEECCCCCEEEECCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCE
TTKMAFDFNTPLLQVQHHHAHFLASILDALLQDPHLNHPFIGIVWDGSGAYENKIYGAEC
EEEEEEECCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCHH
FVGDLERIEEMARFEEFLLLGGQKAIKEPKRLVLEIALKHQLNKLLKRVQKHFKEDELEI
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
FQQMHDKKIQSIATNSIGRLFDIVAFSLDLTGTISFEAESGQVLENLALQSDEIAFYPFT
HHHHHHHHHHHHHHHHHHHHHHHHHHHEECEEEEEEECCCCHHHHHHHCCCCCEEEEEEE
IKNSVVCLKDFYQAFEKDLGVLEPKHIAKKFFNSLVEIITALIAPFKEHVVVCSGGVFCN
ECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHCEEEECCCHHHH
QLLCEQLAKRLRGLKRQYFFHKHFPPNDSSIPIGQALMAYFNPTIIKKG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]