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The map label for this gene is dnaG [H]

Identifier: 108562437

GI number: 108562437

Start: 11222

End: 12901

Strand: Direct

Name: dnaG [H]

Synonym: HPAG1_0012

Alternate gene names: 108562437

Gene position: 11222-12901 (Clockwise)

Preceding gene: 108562433

Following gene: 108562438

Centisome position: 0.7

GC content: 39.52

Gene sequence:

>1680_bases
ATGATTCTTAAAAGTTCCATTGATCGCCTTTTGCAAACGATAGACATTGTAGAAGTCATTAGCTCTTATGTGGATTTGAG
GAAATCCGGTTCTAGCTACATGGCTTGTTGCCCTTTTCATGAAGAAAGGAGCGCGAGTTTTAGCGTCAATCAAATTAAAG
GGTTTTACCATTGCTTTGGGTGTGGGGCGAGCGGGGATAGCATTAAATTCGTGATGGCGTTTGAAAAACTTTCGTTTGTG
GAAGCGTTAGAGAAATTAGCCCACCGATTCAATATAGTTTTAGAGTATGACAAAGGCGCTTATTACGATCATAAAGAAGA
CTACCACCTTTTAGAAATGGTGAGCTCGTTGTATCAAGAAGAGCTTTTTAACGCCCCGTTTTTTTTGAATTATTTGCAAA
AAAGAGGGCTTAGTCTAGAGAGTATAAGAGCGTTTAAATTAGGCTTATGCACGAATAGAATTGATTACGGCATTGAAAAT
AAAGGCCTAAATAAGGATAAACTCATTGAATTAGGCGTGCTAGGCAAGAGCGATAAAAAGGATAAAACCTATTTGCGCTT
TTTGGATCGCATCATGTTCCCTATTTATAGCCCTAGCGCTCAAGTGGTGGGCTTTGGAGGGCGCACCTTAAAAGAAAAAG
CGGCCAAGTATATCAATTCGCCCCAAAATAAGCTTTTTGATAAATCCAGTTTGCTCTATGGCTATCATTTGGCTAAAGAA
CACATCTATAAACAAAAGCAAGTCATTGTAACAGAGGGGTATTTGGATGTGATTTTATTGCATCAGGCGGGTTTTAAAAA
CGCCATAGCCACGCTTGGGACAGCTTTAACGCCATCGCATTTGCCCTTGCTTAAAAAAGGCGAGCCAGAAATTCTTTTGA
GCTATGATGGGGATAAGGCAGGGCGAAACGCAGCTTATAAAGCGAGCTTGATGTTGGCTAAAGAGCAAAGGAGAGGGGGG
GTGATTTTGTTTGAAAACAACCTAGACCCTGCGGATATGATCGCTAATGGCCAGATTGAAATTTTAAAAGACTTGTTATC
GCACTCCATGGCTTTTATTGAGTTTGTTTTAAGGCGCATGGCGGATTCCTATCTTTTAGACGATCCTTTAGAAAAAGATA
AGGCCCTTAAAGAAATGTTAGGGTTTTTAAAAAACTTTTCCTTGCTTTTACAAAGCGAATACAAGCCCTTAATCGCTGCA
CTTTTGCAAGCGCCTTTGCATGTTTTAGGGGTTAGAGAGCGAGCCTCTTTTCAGCCTTTTTACCCCAAAACAGAAAAACC
TAATCTTGCTCAAAAGTTCGCTCATGTTCCTAACACGATGGGTTTGGAATTTTTAGAAAAATTAGTGATCCGCTATCTTT
TAGAAGACAGAAGCTTGTTGGATTTGGCGGTGGGTTATATCCATAGTGGGGTATTCTTGCATAAAAAACAAGAATTTGAC
GCTTTGTGTCAAGAAAAATTGGACGACCCTAAATTAGTTGCGTTATTATTAGATGCGAATTTACCCCTAAAAAAAGGGGG
GTTTGAAAAGGAATTGCGTTTGTTGATTTTGCGCTATTTTGAGCGGCAACTCAAAGAAATCCCTAAAAGTTCGCTTTCTT
TTAGCGAAAAAATGATCTATTTAAAAAAGGCCCGCCAGGCCATTATGAAATTAAAACAAGGAGAATTAGTCGCCATATGA

Upstream 100 bases:

>100_bases
AGAGACGTTATTAACAAACCTTTTTTAAGATTTTATTTATTGAGTATAATTCATTCAAGCGAGTTACAACAAGAAAGCAA
AATTAAAGAAAGCATGAAAA

Downstream 100 bases:

>100_bases
AAAAGATTAAAGCTTTAGCCCTATTTAGTGGGGGGTTGGATAGTTTGTTGTCTATGAAATTACTCATCGATCAAGGCATT
GAGGTAACCGCTTTGCACTT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MILKSSIDRLLQTIDIVEVISSYVDLRKSGSSYMACCPFHEERSASFSVNQIKGFYHCFGCGASGDSIKFVMAFEKLSFV
EALEKLAHRFNIVLEYDKGAYYDHKEDYHLLEMVSSLYQEELFNAPFFLNYLQKRGLSLESIRAFKLGLCTNRIDYGIEN
KGLNKDKLIELGVLGKSDKKDKTYLRFLDRIMFPIYSPSAQVVGFGGRTLKEKAAKYINSPQNKLFDKSSLLYGYHLAKE
HIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEILLSYDGDKAGRNAAYKASLMLAKEQRRGG
VILFENNLDPADMIANGQIEILKDLLSHSMAFIEFVLRRMADSYLLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIAA
LLQAPLHVLGVRERASFQPFYPKTEKPNLAQKFAHVPNTMGLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSSLSFSEKMIYLKKARQAIMKLKQGELVAI

Sequences:

>Translated_559_residues
MILKSSIDRLLQTIDIVEVISSYVDLRKSGSSYMACCPFHEERSASFSVNQIKGFYHCFGCGASGDSIKFVMAFEKLSFV
EALEKLAHRFNIVLEYDKGAYYDHKEDYHLLEMVSSLYQEELFNAPFFLNYLQKRGLSLESIRAFKLGLCTNRIDYGIEN
KGLNKDKLIELGVLGKSDKKDKTYLRFLDRIMFPIYSPSAQVVGFGGRTLKEKAAKYINSPQNKLFDKSSLLYGYHLAKE
HIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEILLSYDGDKAGRNAAYKASLMLAKEQRRGG
VILFENNLDPADMIANGQIEILKDLLSHSMAFIEFVLRRMADSYLLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIAA
LLQAPLHVLGVRERASFQPFYPKTEKPNLAQKFAHVPNTMGLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSSLSFSEKMIYLKKARQAIMKLKQGELVAI
>Mature_559_residues
MILKSSIDRLLQTIDIVEVISSYVDLRKSGSSYMACCPFHEERSASFSVNQIKGFYHCFGCGASGDSIKFVMAFEKLSFV
EALEKLAHRFNIVLEYDKGAYYDHKEDYHLLEMVSSLYQEELFNAPFFLNYLQKRGLSLESIRAFKLGLCTNRIDYGIEN
KGLNKDKLIELGVLGKSDKKDKTYLRFLDRIMFPIYSPSAQVVGFGGRTLKEKAAKYINSPQNKLFDKSSLLYGYHLAKE
HIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEILLSYDGDKAGRNAAYKASLMLAKEQRRGG
VILFENNLDPADMIANGQIEILKDLLSHSMAFIEFVLRRMADSYLLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIAA
LLQAPLHVLGVRERASFQPFYPKTEKPNLAQKFAHVPNTMGLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSSLSFSEKMIYLKKARQAIMKLKQGELVAI

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=377, Percent_Identity=35.5437665782493, Blast_Score=248, Evalue=9e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 63662; Mature: 63662

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILKSSIDRLLQTIDIVEVISSYVDLRKSGSSYMACCPFHEERSASFSVNQIKGFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHEEE
CGASGDSIKFVMAFEKLSFVEALEKLAHRFNIVLEYDKGAYYDHKEDYHLLEMVSSLYQE
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
ELFNAPFFLNYLQKRGLSLESIRAFKLGLCTNRIDYGIENKGLNKDKLIELGVLGKSDKK
HHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEECCCCCCCC
DKTYLRFLDRIMFPIYSPSAQVVGFGGRTLKEKAAKYINSPQNKLFDKSSLLYGYHLAKE
HHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHH
HIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEILLSYDGDKA
HHHHHHHEEEECCHHHHHEEHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCC
GRNAAYKASLMLAKEQRRGGVILFENNLDPADMIANGQIEILKDLLSHSMAFIEFVLRRM
CCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
ADSYLLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIAALLQAPLHVLGVRERASFQPF
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHCCCCCC
YPKTEKPNLAQKFAHVPNTMGLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
CCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECHHHHH
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSSLSFSEKMIY
HHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKKARQAIMKLKQGELVAI
HHHHHHHHHHHCCCCEEEC
>Mature Secondary Structure
MILKSSIDRLLQTIDIVEVISSYVDLRKSGSSYMACCPFHEERSASFSVNQIKGFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHEEE
CGASGDSIKFVMAFEKLSFVEALEKLAHRFNIVLEYDKGAYYDHKEDYHLLEMVSSLYQE
CCCCCCCEEEEHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
ELFNAPFFLNYLQKRGLSLESIRAFKLGLCTNRIDYGIENKGLNKDKLIELGVLGKSDKK
HHCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHEEEEECCCCCCCC
DKTYLRFLDRIMFPIYSPSAQVVGFGGRTLKEKAAKYINSPQNKLFDKSSLLYGYHLAKE
HHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHH
HIYKQKQVIVTEGYLDVILLHQAGFKNAIATLGTALTPSHLPLLKKGEPEILLSYDGDKA
HHHHHHHEEEECCHHHHHEEHHCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCC
GRNAAYKASLMLAKEQRRGGVILFENNLDPADMIANGQIEILKDLLSHSMAFIEFVLRRM
CCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
ADSYLLDDPLEKDKALKEMLGFLKNFSLLLQSEYKPLIAALLQAPLHVLGVRERASFQPF
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCHHHCCCCCC
YPKTEKPNLAQKFAHVPNTMGLEFLEKLVIRYLLEDRSLLDLAVGYIHSGVFLHKKQEFD
CCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEECHHHHH
ALCQEKLDDPKLVALLLDANLPLKKGGFEKELRLLILRYFERQLKEIPKSSLSFSEKMIY
HHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
LKKARQAIMKLKQGELVAI
HHHHHHHHHHHCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]