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The map label for this gene is pyrF [H]

Identifier: 108562430

GI number: 108562430

Start: 4032

End: 4715

Strand: Direct

Name: pyrF [H]

Synonym: HPAG1_0005

Alternate gene names: 108562430

Gene position: 4032-4715 (Clockwise)

Preceding gene: NA

Following gene: 108562431

Centisome position: 0.25

GC content: 40.06

Gene sequence:

>684_bases
ATGCAATTATGTGTCGCATTGGATTTAGAAAAAAAAGAGGACAATCTTTCTTTATTGCAAGAATTAAAGGGCTTAGATTT
ATGGGCTAAGGTGGGGCTTAGATCTTTTATAAGAGATGGGGCTGTTTTTTTAGATGAAATCAGAAAGATTGATGGGAATT
TTAAGATTTTTTTGGATTTGAAGCTCTATGATATTCCTTATACGATGGCAAATGCCGCGCTAGAATGCGCGAAATTAGAA
ATTGATATGCTCACCGTGCATTTGAGCAGCGCTAAAAGCGCGCTAACCATTTTAATGCAACGCCTGAACGCTCTTAAAAA
ACGCCCCTTGATTATGGGCGTGAGCGCTTTAACCAGCTTTAGTGAAGAGGAATTTTTGATGGTGTATAACGCCCCTTTAA
AAACGCAAGCGATCACATTAAGCGCTATGGGTAAAGAGAGCGGGATTGATGGGGTGGTGTGTTCGGTGTTTGAAAGCTTA
GCGATTAAAGAGGCTTTAGGTCAAGGCTTTTTGACTTTAACCCCTGGCATAAGGCTGGATAAAAACGATAAAGAAGATCA
AGAAAGGGTGGCGAACGCTAAAGAGGCTAAACAAAATTTAAGCGATTTTATCGTGGTAGGCCGCCCCATTTATCATGCTA
AAGAGCCTAGAGAAGTGGTTTTAGAGCTTTTAAAGGATTGTTAA

Upstream 100 bases:

>100_bases
TTGATTAAAATTCGTTTTGTTTTTAAAACGCTTATCATAGCTAAAAATCTTGCATTTCTTTTTGTTATAATGGCGTTATT
TTACACTTTAAAGGGCTTTC

Downstream 100 bases:

>100_bases
ATGCGGGTGTTAGAAACGATTGCTGCTTTAAGAGAATATCGTAAAAATTTGGAAGAAAGCGTGGGGTTTGTGCCGACTAT
GGGGGCTTTACACAAAGGGC

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MQLCVALDLEKKEDNLSLLQELKGLDLWAKVGLRSFIRDGAVFLDEIRKIDGNFKIFLDLKLYDIPYTMANAALECAKLE
IDMLTVHLSSAKSALTILMQRLNALKKRPLIMGVSALTSFSEEEFLMVYNAPLKTQAITLSAMGKESGIDGVVCSVFESL
AIKEALGQGFLTLTPGIRLDKNDKEDQERVANAKEAKQNLSDFIVVGRPIYHAKEPREVVLELLKDC

Sequences:

>Translated_227_residues
MQLCVALDLEKKEDNLSLLQELKGLDLWAKVGLRSFIRDGAVFLDEIRKIDGNFKIFLDLKLYDIPYTMANAALECAKLE
IDMLTVHLSSAKSALTILMQRLNALKKRPLIMGVSALTSFSEEEFLMVYNAPLKTQAITLSAMGKESGIDGVVCSVFESL
AIKEALGQGFLTLTPGIRLDKNDKEDQERVANAKEAKQNLSDFIVVGRPIYHAKEPREVVLELLKDC
>Mature_227_residues
MQLCVALDLEKKEDNLSLLQELKGLDLWAKVGLRSFIRDGAVFLDEIRKIDGNFKIFLDLKLYDIPYTMANAALECAKLE
IDMLTVHLSSAKSALTILMQRLNALKKRPLIMGVSALTSFSEEEFLMVYNAPLKTQAITLSAMGKESGIDGVVCSVFESL
AIKEALGQGFLTLTPGIRLDKNDKEDQERVANAKEAKQNLSDFIVVGRPIYHAKEPREVVLELLKDC

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP) [H]

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787537, Length=221, Percent_Identity=33.4841628959276, Blast_Score=112, Evalue=2e-26,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00215 OMPdecase [H]

EC number: =4.1.1.23 [H]

Molecular weight: Translated: 25292; Mature: 25292

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00156 OMPDECASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLCVALDLEKKEDNLSLLQELKGLDLWAKVGLRSFIRDGAVFLDEIRKIDGNFKIFLDL
CCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEE
KLYDIPYTMANAALECAKLEIDMLTVHLSSAKSALTILMQRLNALKKRPLIMGVSALTSF
EEEECCHHHHHHHHHHHHEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCC
SEEEFLMVYNAPLKTQAITLSAMGKESGIDGVVCSVFESLAIKEALGQGFLTLTPGIRLD
CCCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCEEEC
KNDKEDQERVANAKEAKQNLSDFIVVGRPIYHAKEPREVVLELLKDC
CCCCHHHHHHHHHHHHHHHHHHEEEECCCHHCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQLCVALDLEKKEDNLSLLQELKGLDLWAKVGLRSFIRDGAVFLDEIRKIDGNFKIFLDL
CCEEEEECCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEE
KLYDIPYTMANAALECAKLEIDMLTVHLSSAKSALTILMQRLNALKKRPLIMGVSALTSF
EEEECCHHHHHHHHHHHHEEEEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHCC
SEEEFLMVYNAPLKTQAITLSAMGKESGIDGVVCSVFESLAIKEALGQGFLTLTPGIRLD
CCCCEEEEECCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCEEEC
KNDKEDQERVANAKEAKQNLSDFIVVGRPIYHAKEPREVVLELLKDC
CCCCHHHHHHHHHHHHHHHHHHEEEECCCHHCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA