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The map label for this gene is purR [H]

Identifier: 107023073

GI number: 107023073

Start: 1691421

End: 1692587

Strand: Reverse

Name: purR [H]

Synonym: Bcen_1521

Alternate gene names: 107023073

Gene position: 1692587-1691421 (Counterclockwise)

Preceding gene: 107023074

Following gene: 107023069

Centisome position: 51.38

GC content: 67.87

Gene sequence:

>1167_bases
GTGAAGGGTTCGGCGTGTATCCCTGTAAAATGCGGCATTGCCAGCCCACAGGCGGATGCCTTCCCATCGATGAAGCGTGC
CGTATCCATGAAGGACGTCGCATTGGCCGCCGGCGTCTCTCAGCCCGCGGTCTCCTATGCGTACAACAAGCCGTCGGAAA
TCTCCGAGGCGCAGCGCGAGCATATTTTCGCCGTGGCGAAAAAACTCGGCTATCCCGGGCCCAACGTCCGCGGGCGCAGC
CTGCGCTCCGGGCGGGTCGGTGCCGTCGGCCTGATGATCATGGACGAGTTGCCTTACGCATTCGCCGATCCGGCGACCAC
CTGCCTGCTGCGCGGCATCGGTGAAATCAGCGAGCTCGCGAACGTCGCGCTCGCGCTCTTCCCGCTACAGAAGGATCTGG
CGAATCATGCCGGCGACGCCGCGAACGGCAGCCTCGCGGTACGCGGGTTCGTCGACGGCCTGATCGCGTACTCGCTGCCG
GACCACTATCCGGTCATCGAGGTCGCGCGCCGGCAGGGCATCCCGGTCGTGATCGTGGACGCACCGATCATCGACAAGGT
GCCGTTCGTCGGGATCGACGATCTGCAATCCGCCATCGTCCAGATGCGGCATCTGGTCGAGCTCGGTCACCGGCGGATCG
GCATCGTGGCCGACCGGCTGCGTCCGGACGGCGTCAGGGGCATCGTCAGCAGGCAGCGCTTCCAGGAGGCCACGGAAAAC
GTCGTGCGCGCCCGCATCGACGGCTATCTGCGCGCGGCCGAAGCGGTGGGGCTCGGCTTCGACGACCTGTTGATCGTCGA
GGCCGGCGGCTTCGATCGTGCATCGGGCATCTGGGCGGCCGAACAGATGCTGCAGCATACGGGCCTCACGGCAGTGGCGG
CGACGTCGGACGTGATGGCGCTCGCGCTCGTGCAGGCGGCGCGTGAACGCGGGGTGGCGGTGCCCGATCGGCTGTCCGTG
GCGGGGTTCGACGATATACCGGAAGCAGCCGGCGCAGGGCTCACGACCATCCGCCAGCCGCTGATCGAGAAAGGCCGCCA
GGCCGCGCAACTGCTCAGCGAACTGCTGAGTGGCCCCGCCGCCCGCAAGCCGCGCGTGCCCAGGGTGCTCCTGCCGACGG
AGCTGGTGGTGCGATCGACGACGCAGGCGCCGGCCGCGGCAAGATGA

Upstream 100 bases:

>100_bases
GCGGACGACGATTGACCGAGCGCTCGGCCGGCGACGTCGTCGCGGCGTCGAGCGGCGCGCTTCATGCAAGCGTGATGGCA
TTGCTGGCCGGCTCGAATGC

Downstream 100 bases:

>100_bases
GCGCGGCCGGCGGATCGCGGCGCAGGGGCAAGCGTCGCTTCAGCGACCGACGAACTGCCTGGCCACACGAAGCGCATTCG
CGGCGATCGTGAGCGCCGGA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MKGSACIPVKCGIASPQADAFPSMKRAVSMKDVALAAGVSQPAVSYAYNKPSEISEAQREHIFAVAKKLGYPGPNVRGRS
LRSGRVGAVGLMIMDELPYAFADPATTCLLRGIGEISELANVALALFPLQKDLANHAGDAANGSLAVRGFVDGLIAYSLP
DHYPVIEVARRQGIPVVIVDAPIIDKVPFVGIDDLQSAIVQMRHLVELGHRRIGIVADRLRPDGVRGIVSRQRFQEATEN
VVRARIDGYLRAAEAVGLGFDDLLIVEAGGFDRASGIWAAEQMLQHTGLTAVAATSDVMALALVQAARERGVAVPDRLSV
AGFDDIPEAAGAGLTTIRQPLIEKGRQAAQLLSELLSGPAARKPRVPRVLLPTELVVRSTTQAPAAAR

Sequences:

>Translated_388_residues
MKGSACIPVKCGIASPQADAFPSMKRAVSMKDVALAAGVSQPAVSYAYNKPSEISEAQREHIFAVAKKLGYPGPNVRGRS
LRSGRVGAVGLMIMDELPYAFADPATTCLLRGIGEISELANVALALFPLQKDLANHAGDAANGSLAVRGFVDGLIAYSLP
DHYPVIEVARRQGIPVVIVDAPIIDKVPFVGIDDLQSAIVQMRHLVELGHRRIGIVADRLRPDGVRGIVSRQRFQEATEN
VVRARIDGYLRAAEAVGLGFDDLLIVEAGGFDRASGIWAAEQMLQHTGLTAVAATSDVMALALVQAARERGVAVPDRLSV
AGFDDIPEAAGAGLTTIRQPLIEKGRQAAQLLSELLSGPAARKPRVPRVLLPTELVVRSTTQAPAAAR
>Mature_388_residues
MKGSACIPVKCGIASPQADAFPSMKRAVSMKDVALAAGVSQPAVSYAYNKPSEISEAQREHIFAVAKKLGYPGPNVRGRS
LRSGRVGAVGLMIMDELPYAFADPATTCLLRGIGEISELANVALALFPLQKDLANHAGDAANGSLAVRGFVDGLIAYSLP
DHYPVIEVARRQGIPVVIVDAPIIDKVPFVGIDDLQSAIVQMRHLVELGHRRIGIVADRLRPDGVRGIVSRQRFQEATEN
VVRARIDGYLRAAEAVGLGFDDLLIVEAGGFDRASGIWAAEQMLQHTGLTAVAATSDVMALALVQAARERGVAVPDRLSV
AGFDDIPEAAGAGLTTIRQPLIEKGRQAAQLLSELLSGPAARKPRVPRVLLPTELVVRSTTQAPAAAR

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=372, Percent_Identity=30.1075268817204, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1790194, Length=347, Percent_Identity=28.8184438040346, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787948, Length=342, Percent_Identity=28.6549707602339, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787580, Length=358, Percent_Identity=25.4189944134078, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789202, Length=369, Percent_Identity=26.0162601626016, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786540, Length=373, Percent_Identity=26.5415549597855, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1787906, Length=325, Percent_Identity=27.0769230769231, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 41074; Mature: 41074

Theoretical pI: Translated: 8.79; Mature: 8.79

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGSACIPVKCGIASPQADAFPSMKRAVSMKDVALAAGVSQPAVSYAYNKPSEISEAQRE
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHH
HIFAVAKKLGYPGPNVRGRSLRSGRVGAVGLMIMDELPYAFADPATTCLLRGIGEISELA
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHH
NVALALFPLQKDLANHAGDAANGSLAVRGFVDGLIAYSLPDHYPVIEVARRQGIPVVIVD
HHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEC
APIIDKVPFVGIDDLQSAIVQMRHLVELGHRRIGIVADRLRPDGVRGIVSRQRFQEATEN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHH
VVRARIDGYLRAAEAVGLGFDDLLIVEAGGFDRASGIWAAEQMLQHTGLTAVAATSDVMA
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
LALVQAARERGVAVPDRLSVAGFDDIPEAAGAGLTTIRQPLIEKGRQAAQLLSELLSGPA
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ARKPRVPRVLLPTELVVRSTTQAPAAAR
CCCCCCCEEECCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKGSACIPVKCGIASPQADAFPSMKRAVSMKDVALAAGVSQPAVSYAYNKPSEISEAQRE
CCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCHHHHHHHHH
HIFAVAKKLGYPGPNVRGRSLRSGRVGAVGLMIMDELPYAFADPATTCLLRGIGEISELA
HHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHCCHHHHHHHHHHHHHHHHH
NVALALFPLQKDLANHAGDAANGSLAVRGFVDGLIAYSLPDHYPVIEVARRQGIPVVIVD
HHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEC
APIIDKVPFVGIDDLQSAIVQMRHLVELGHRRIGIVADRLRPDGVRGIVSRQRFQEATEN
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHHHCCCCCHHHHHHHHHHHHHHHHH
VVRARIDGYLRAAEAVGLGFDDLLIVEAGGFDRASGIWAAEQMLQHTGLTAVAATSDVMA
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHH
LALVQAARERGVAVPDRLSVAGFDDIPEAAGAGLTTIRQPLIEKGRQAAQLLSELLSGPA
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCC
ARKPRVPRVLLPTELVVRSTTQAPAAAR
CCCCCCCEEECCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA