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The map label for this gene is rfaF [H]

Identifier: 107022933

GI number: 107022933

Start: 1518365

End: 1519453

Strand: Reverse

Name: rfaF [H]

Synonym: Bcen_1381

Alternate gene names: 107022933

Gene position: 1519453-1518365 (Counterclockwise)

Preceding gene: 107022934

Following gene: 107022932

Centisome position: 46.12

GC content: 72.08

Gene sequence:

>1089_bases
ATGAAGCTCGATCCCGGCGTCGATTCCGACGGCTCGCGCGGCCCGAAGCGGATCGCGGTGTTCCGCGCGTTGCAGCTCGG
CGACATGCTGTGCGCGGTGCCCGCGCTGCGCGCGCTGCGACGCGGCGAACCCGACGCGCGCATCACGCTGATCGGCCTGC
CGTGGGCGCGCGAATTCGCATCGCGCTTCTCGGACTACATCGACAGCTTCATCGCATTCCCCGGCGCGCCGGGTCTCGGC
GAACAGCCCGAACCCGACGCGGCCACGCGCGAGGCATTCATCGCCGAATGCCGCTCGCGCGACTTCGATCTCGCGATCCA
GCTGCACGGCAGCGGCGCACACACGAACGCGATCGTTGCGTCGCTCGGCGCGACGCGCACGGCCGGCTTCGTGCCGGCCG
ACGGCGGCACGGCCGCCCTCGACTGCGCGCTCGTGTGGCGCGACGACGAACCCGAAGTGACGCGCTACCTGGCGCTCACG
CGCAAGCTCGGCTACGCGGACTGGGGCGACTACCTCGAATTCCCGCTCGGCGGCCTCGACTACGCGCTGTGGCACGTGCT
CTGCGACGAGCACTCGCTCGACCCCGGCCGTTACGTGATCGTCCATCCCGGCGCGCGGATGGCGTCGCGACGCTGGCCGG
TCGAACGCTTCGCGCACGCCGCGCGGCAGCTCGCCGACGACGGCTGGCAGATCGTGCTGACCGGCACGCGCACGGAACTG
GCGCTGGCGAGCGCGTTCGCCGAACACCTCGGGCGGCCGTGCGTGAACCTGTGCGGCCGCACGCCGCTCGGCGCGCTGGC
CGCGCTGATCGCCCGCGCGCGGCTGCTGTTGTGCAACGACACGGGCGTATCGCACGTCGCGGCGGCGCTCGGCACGCCGA
GCATCGTCGTCGCGTGCGGCAGCGATACGGCCCGCTGGGCGCCGCTCGACACCGAGCGTCATCATGTGCTCGCGAACTAC
CCGCCGTGCCGGCCGTGCATGTTCGATACGTGCCCGTACGGGCACGAGTGCGCGACGGCGATCGGCGTCGGCGACGTGAT
GGACCGGGCGGGCGAGCTGCTCGCGGAGGAGAGACGTCATGTCACGTAA

Upstream 100 bases:

>100_bases
TGTCGCATGTGGCCGAGATGATCGTCACGTCCATCGCGATTCCGCCCGTGTCGCTGTACTGGCGCATGCGCGGCGCGCTT
CACTTCAGAGTGTTATTCCT

Downstream 100 bases:

>100_bases
GCGGTTGCGCGTGCTCACCTGGCACGTGCATGGCAACTACCTGTATTACCTGTCGCAGGCGCCGCACGATTTCTACATCG
TGACGAAACCGCAGCATCCG

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MKLDPGVDSDGSRGPKRIAVFRALQLGDMLCAVPALRALRRGEPDARITLIGLPWAREFASRFSDYIDSFIAFPGAPGLG
EQPEPDAATREAFIAECRSRDFDLAIQLHGSGAHTNAIVASLGATRTAGFVPADGGTAALDCALVWRDDEPEVTRYLALT
RKLGYADWGDYLEFPLGGLDYALWHVLCDEHSLDPGRYVIVHPGARMASRRWPVERFAHAARQLADDGWQIVLTGTRTEL
ALASAFAEHLGRPCVNLCGRTPLGALAALIARARLLLCNDTGVSHVAAALGTPSIVVACGSDTARWAPLDTERHHVLANY
PPCRPCMFDTCPYGHECATAIGVGDVMDRAGELLAEERRHVT

Sequences:

>Translated_362_residues
MKLDPGVDSDGSRGPKRIAVFRALQLGDMLCAVPALRALRRGEPDARITLIGLPWAREFASRFSDYIDSFIAFPGAPGLG
EQPEPDAATREAFIAECRSRDFDLAIQLHGSGAHTNAIVASLGATRTAGFVPADGGTAALDCALVWRDDEPEVTRYLALT
RKLGYADWGDYLEFPLGGLDYALWHVLCDEHSLDPGRYVIVHPGARMASRRWPVERFAHAARQLADDGWQIVLTGTRTEL
ALASAFAEHLGRPCVNLCGRTPLGALAALIARARLLLCNDTGVSHVAAALGTPSIVVACGSDTARWAPLDTERHHVLANY
PPCRPCMFDTCPYGHECATAIGVGDVMDRAGELLAEERRHVT
>Mature_362_residues
MKLDPGVDSDGSRGPKRIAVFRALQLGDMLCAVPALRALRRGEPDARITLIGLPWAREFASRFSDYIDSFIAFPGAPGLG
EQPEPDAATREAFIAECRSRDFDLAIQLHGSGAHTNAIVASLGATRTAGFVPADGGTAALDCALVWRDDEPEVTRYLALT
RKLGYADWGDYLEFPLGGLDYALWHVLCDEHSLDPGRYVIVHPGARMASRRWPVERFAHAARQLADDGWQIVLTGTRTEL
ALASAFAEHLGRPCVNLCGRTPLGALAALIARARLLLCNDTGVSHVAAALGTPSIVVACGSDTARWAPLDTERHHVLANY
PPCRPCMFDTCPYGHECATAIGVGDVMDRAGELLAEERRHVT

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=365, Percent_Identity=26.8493150684932, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 39103; Mature: 39103

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLDPGVDSDGSRGPKRIAVFRALQLGDMLCAVPALRALRRGEPDARITLIGLPWAREFA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHH
SRFSDYIDSFIAFPGAPGLGEQPEPDAATREAFIAECRSRDFDLAIQLHGSGAHTNAIVA
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
SLGATRTAGFVPADGGTAALDCALVWRDDEPEVTRYLALTRKLGYADWGDYLEFPLGGLD
HHCCCCCCCEEECCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHCCCCHHH
YALWHVLCDEHSLDPGRYVIVHPGARMASRRWPVERFAHAARQLADDGWQIVLTGTRTEL
HHHHHHHHCCCCCCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCHHH
ALASAFAEHLGRPCVNLCGRTPLGALAALIARARLLLCNDTGVSHVAAALGTPSIVVACG
HHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCEEEEEEC
SDTARWAPLDTERHHVLANYPPCRPCMFDTCPYGHECATAIGVGDVMDRAGELLAEERRH
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
VT
CC
>Mature Secondary Structure
MKLDPGVDSDGSRGPKRIAVFRALQLGDMLCAVPALRALRRGEPDARITLIGLPWAREFA
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHH
SRFSDYIDSFIAFPGAPGLGEQPEPDAATREAFIAECRSRDFDLAIQLHGSGAHTNAIVA
HHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
SLGATRTAGFVPADGGTAALDCALVWRDDEPEVTRYLALTRKLGYADWGDYLEFPLGGLD
HHCCCCCCCEEECCCCCEEEEEEEEEECCCHHHHHHHHHHHHCCCCCCCHHHHCCCCHHH
YALWHVLCDEHSLDPGRYVIVHPGARMASRRWPVERFAHAARQLADDGWQIVLTGTRTEL
HHHHHHHHCCCCCCCCCEEEECCCCHHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCHHH
ALASAFAEHLGRPCVNLCGRTPLGALAALIARARLLLCNDTGVSHVAAALGTPSIVVACG
HHHHHHHHHHCCHHHHHCCCCCHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCEEEEEEC
SDTARWAPLDTERHHVLANYPPCRPCMFDTCPYGHECATAIGVGDVMDRAGELLAEERRH
CCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
VT
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]