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The map label for this gene is livK [C]

Identifier: 107022242

GI number: 107022242

Start: 732482

End: 733867

Strand: Reverse

Name: livK [C]

Synonym: Bcen_0686

Alternate gene names: 107022242

Gene position: 733867-732482 (Counterclockwise)

Preceding gene: 107022243

Following gene: 107022240

Centisome position: 22.28

GC content: 64.86

Gene sequence:

>1386_bases
ATGCGCAAATCCGCATCGAATGCGCGACAAATTTCCAAGACCCGCGCATATCGGCGACCTATATATCGAAGCAATCGGGG
CTCTTCGCGGAGCCTGACGGGAGATGTTTCCGAGGATCACGATGCCGACCTCCCCGCCCGATTCCGAGAAGCGGTCGAAC
CGCCCGCCCGTCGCACGCGTCCGGCCCGCCCAGGCGCGCGCGGTACCCACTCGCCGGTGTCGAACAAGAGGAACAACATG
AAATTCCGTCATTCCCTGCTGTCGGTGTCGATTGCATCCGCGCTTGCCCTTCTGTCGCAACAGGCTGCCCAGGCCGCCGA
CACAACCGACGTGAAGGTCGGCTTCGCCGCGCCGCTCACGGGCGTGAACGCCGGCTACGGCAAGGACCTGCAAAACGGCG
TGCAGCTCGCGCTCGACGATGCAGCCGCGCAGAAAGTGCAGATCGCCGGCAAGCCTGCCCGCTTCAATCTCGTCGTGCAG
GACGACCAGGCCGACCCGCGCATCGGCGTGCAGGCCGCGCAGGCACTGGTCGACCAGAACGTGTCGGTCGTGGTCGGTCA
CTTCAATTCGGGGACGACGATTCCGGCGTCGGTGGTCTACGACAAGGCCGGCATTCCGGTGATCGACCCGGCCGCGACCA
ACCCGACGCTCACGTCGCGCGGGCTCGCGAACATGTTCATGGTGATCGCGACCGATGGCCAGAATGCCGGCAACGCGGGC
AAGTACGCGGTCGAGGTGACGAAGGCGAAGCGCATCGCGATCATCGACGACCGCACCGCGTTCGGCCAGGGCGAAGCCGA
CGAATTCGAGAAGGCCGTGAAGGCCGCCGGCGGCAACCTCATCGGTCGCGAGTTCACCAGCAACCAGGCGGTCGATTTCC
GCGCGCAGATCACGAGCCTGAAGGCCAAGAATCCCGACCTGATCTTCTTCGGCGGCCTCGATTCGCTCGCCGCCAACTTC
ATCAAGCAAATGCGTCAACTCGGGCTGAACGCGCAATTCGTCGGCGGCGGCGGCGTGAAGGACAACGAGTTCATCAAGAT
CGCGGGGCCGGCCGCCGAAGGCGCGATGGCGTGGGAATACGGTCAGCCGCTCGACGAGCTGCCGCAGGGGAAGGATTTCG
AGGCGCGCTTCAAGAAGCGCTTCGGCGTCGACGTGCTGTCGTATGCGCAGTTCGGCTATGACGCGGCGTGGGCGGCGATC
AAGGCGATGCAGGCGGCCGGTTCGACCGATCCGAAGGCCTATCGCCCGGCGCTGAAGAAAATCGACTTCCCAGGCGTGAC
CGGCCGCATCTCGTTCGCGAACGACGGCTCGCTGAAGAGCGGGATGTCGACGCTGTACCAGGTGAAGAACGGCGCGTGGA
AGACGATCGTGACGAAGGGAGGCTGA

Upstream 100 bases:

>100_bases
GCCGCGCCGCGTTTTTGCCCGCACCGCCCCTCTTCTCTGCGCCCGTTGCGTTCGCGACGGGAAACTCCCGATACCGGCCG
CCCGCGCACGCGCGGAAGCT

Downstream 100 bases:

>100_bases
TCGGCGAGAGGGGCGGCCGGCGGCGGGAACGCCGTCTGGCCGCGGAGATCGAGACGATTGCGCCGATCTCGTATGACGAC
TGATGTTAAAACAATGCCAG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRKSASNARQISKTRAYRRPIYRSNRGSSRSLTGDVSEDHDADLPARFREAVEPPARRTRPARPGARGTHSPVSNKRNNM
KFRHSLLSVSIASALALLSQQAAQAADTTDVKVGFAAPLTGVNAGYGKDLQNGVQLALDDAAAQKVQIAGKPARFNLVVQ
DDQADPRIGVQAAQALVDQNVSVVVGHFNSGTTIPASVVYDKAGIPVIDPAATNPTLTSRGLANMFMVIATDGQNAGNAG
KYAVEVTKAKRIAIIDDRTAFGQGEADEFEKAVKAAGGNLIGREFTSNQAVDFRAQITSLKAKNPDLIFFGGLDSLAANF
IKQMRQLGLNAQFVGGGGVKDNEFIKIAGPAAEGAMAWEYGQPLDELPQGKDFEARFKKRFGVDVLSYAQFGYDAAWAAI
KAMQAAGSTDPKAYRPALKKIDFPGVTGRISFANDGSLKSGMSTLYQVKNGAWKTIVTKGG

Sequences:

>Translated_461_residues
MRKSASNARQISKTRAYRRPIYRSNRGSSRSLTGDVSEDHDADLPARFREAVEPPARRTRPARPGARGTHSPVSNKRNNM
KFRHSLLSVSIASALALLSQQAAQAADTTDVKVGFAAPLTGVNAGYGKDLQNGVQLALDDAAAQKVQIAGKPARFNLVVQ
DDQADPRIGVQAAQALVDQNVSVVVGHFNSGTTIPASVVYDKAGIPVIDPAATNPTLTSRGLANMFMVIATDGQNAGNAG
KYAVEVTKAKRIAIIDDRTAFGQGEADEFEKAVKAAGGNLIGREFTSNQAVDFRAQITSLKAKNPDLIFFGGLDSLAANF
IKQMRQLGLNAQFVGGGGVKDNEFIKIAGPAAEGAMAWEYGQPLDELPQGKDFEARFKKRFGVDVLSYAQFGYDAAWAAI
KAMQAAGSTDPKAYRPALKKIDFPGVTGRISFANDGSLKSGMSTLYQVKNGAWKTIVTKGG
>Mature_461_residues
MRKSASNARQISKTRAYRRPIYRSNRGSSRSLTGDVSEDHDADLPARFREAVEPPARRTRPARPGARGTHSPVSNKRNNM
KFRHSLLSVSIASALALLSQQAAQAADTTDVKVGFAAPLTGVNAGYGKDLQNGVQLALDDAAAQKVQIAGKPARFNLVVQ
DDQADPRIGVQAAQALVDQNVSVVVGHFNSGTTIPASVVYDKAGIPVIDPAATNPTLTSRGLANMFMVIATDGQNAGNAG
KYAVEVTKAKRIAIIDDRTAFGQGEADEFEKAVKAAGGNLIGREFTSNQAVDFRAQITSLKAKNPDLIFFGGLDSLAANF
IKQMRQLGLNAQFVGGGGVKDNEFIKIAGPAAEGAMAWEYGQPLDELPQGKDFEARFKKRFGVDVLSYAQFGYDAAWAAI
KAMQAAGSTDPKAYRPALKKIDFPGVTGRISFANDGSLKSGMSTLYQVKNGAWKTIVTKGG

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=349, Percent_Identity=32.378223495702, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI48994941, Length=355, Percent_Identity=30.1408450704225, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 49064; Mature: 49064

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSASNARQISKTRAYRRPIYRSNRGSSRSLTGDVSEDHDADLPARFREAVEPPARRTR
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHCC
PARPGARGTHSPVSNKRNNMKFRHSLLSVSIASALALLSQQAAQAADTTDVKVGFAAPLT
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GVNAGYGKDLQNGVQLALDDAAAQKVQIAGKPARFNLVVQDDQADPRIGVQAAQALVDQN
CCCCCCCCCHHHCCEEEECHHHHHEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHCCC
VSVVVGHFNSGTTIPASVVYDKAGIPVIDPAATNPTLTSRGLANMFMVIATDGQNAGNAG
CEEEEEECCCCCCCCHHEEECCCCCCEECCCCCCCCCHHCCCCEEEEEEEECCCCCCCCC
KYAVEVTKAKRIAIIDDRTAFGQGEADEFEKAVKAAGGNLIGREFTSNQAVDFRAQITSL
CEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCEEHHHHHHHH
KAKNPDLIFFGGLDSLAANFIKQMRQLGLNAQFVGGGGVKDNEFIKIAGPAAEGAMAWEY
CCCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCEEECC
GQPLDELPQGKDFEARFKKRFGVDVLSYAQFGYDAAWAAIKAMQAAGSTDPKAYRPALKK
CCCHHHCCCCCCHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IDFPGVTGRISFANDGSLKSGMSTLYQVKNGAWKTIVTKGG
CCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCC
>Mature Secondary Structure
MRKSASNARQISKTRAYRRPIYRSNRGSSRSLTGDVSEDHDADLPARFREAVEPPARRTR
CCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHCC
PARPGARGTHSPVSNKRNNMKFRHSLLSVSIASALALLSQQAAQAADTTDVKVGFAAPLT
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCC
GVNAGYGKDLQNGVQLALDDAAAQKVQIAGKPARFNLVVQDDQADPRIGVQAAQALVDQN
CCCCCCCCCHHHCCEEEECHHHHHEEEECCCCEEEEEEEECCCCCCCHHHHHHHHHHCCC
VSVVVGHFNSGTTIPASVVYDKAGIPVIDPAATNPTLTSRGLANMFMVIATDGQNAGNAG
CEEEEEECCCCCCCCHHEEECCCCCCEECCCCCCCCCHHCCCCEEEEEEEECCCCCCCCC
KYAVEVTKAKRIAIIDDRTAFGQGEADEFEKAVKAAGGNLIGREFTSNQAVDFRAQITSL
CEEEEEECCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCEEHHHHHHHH
KAKNPDLIFFGGLDSLAANFIKQMRQLGLNAQFVGGGGVKDNEFIKIAGPAAEGAMAWEY
CCCCCCEEEECCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEECCCCCCCEEECC
GQPLDELPQGKDFEARFKKRFGVDVLSYAQFGYDAAWAAIKAMQAAGSTDPKAYRPALKK
CCCHHHCCCCCCHHHHHHHHHCCHHHHHHHHCHHHHHHHHHHHHHCCCCCCHHHHHHHHH
IDFPGVTGRISFANDGSLKSGMSTLYQVKNGAWKTIVTKGG
CCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA