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The map label for this gene is dfrA [H]

Identifier: 107022160

GI number: 107022160

Start: 649319

End: 649879

Strand: Reverse

Name: dfrA [H]

Synonym: Bcen_0603

Alternate gene names: 107022160

Gene position: 649879-649319 (Counterclockwise)

Preceding gene: 107022161

Following gene: 107022158

Centisome position: 19.73

GC content: 67.2

Gene sequence:

>561_bases
GTGCGCGCCACCGTTCCGGTAAAATCGGCCCCATTCCGTTTCCCCTCTTCCCCGACCTTCATGACGACGTTGACCCTGAT
CGTCGCGCGTGCCCGCAACGGCATCATCGGCCGCGACAACCAGTTGCCCTGGAAACTTCCCGAGGATCTCGCGTTCTTCA
AGCGCACGACGATGGGCGCGCCGATCGTGATGGGCCGCAAGACCCACGAGTCGATCGGCCGGCCGCTGCCGGGCCGCCGC
AACATCGTCGTCACGCGCGACGCCACGCGCCGTTTCGAAGGCTGCGACACGGTCACGTCGCTCGCCGATGCGCTGGCGCT
CGCCGCGCGCGACGGGGTGCCCGAGGCATTCCTGATCGGCGGTGCACAACTCTATGCGGAAGGTCTCATGCTCGCCGACA
AGCTGATCGTCACCGAGATCGACGCCGACTTCGACGGCGGCGCATCGTTCCCGGCGCCCGATGCCGCACACTGGCAGGAA
GTCTCGCGCGACGCGCATCAGGCTGCCGCGCCCAACACGTTCGGCTATGCGTTCGTCGTCTACACGCGCAAGCGCGACTG
A

Upstream 100 bases:

>100_bases
TTTTTATTCGGACGGTCGTCGCGACGCGAGGCATCACGCGTCGCGACGCACGGCGCATCGCACAAGAAGCGCCGACGCCC
ATGTGGATGCGGGAAATCCG

Downstream 100 bases:

>100_bases
CGACTTCTTCGCACGATGAAAAAAGCCCGACCGGCTCGCGCCGGTCGGGCTTTGTCGTTTCCTGGCGGCCTGCGCCGCGT
GCGGCTTACTGCCCTGCGAT

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MRATVPVKSAPFRFPSSPTFMTTLTLIVARARNGIIGRDNQLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRR
NIVVTRDATRRFEGCDTVTSLADALALAARDGVPEAFLIGGAQLYAEGLMLADKLIVTEIDADFDGGASFPAPDAAHWQE
VSRDAHQAAAPNTFGYAFVVYTRKRD

Sequences:

>Translated_186_residues
MRATVPVKSAPFRFPSSPTFMTTLTLIVARARNGIIGRDNQLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRR
NIVVTRDATRRFEGCDTVTSLADALALAARDGVPEAFLIGGAQLYAEGLMLADKLIVTEIDADFDGGASFPAPDAAHWQE
VSRDAHQAAAPNTFGYAFVVYTRKRD
>Mature_186_residues
MRATVPVKSAPFRFPSSPTFMTTLTLIVARARNGIIGRDNQLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRR
NIVVTRDATRRFEGCDTVTSLADALALAARDGVPEAFLIGGAQLYAEGLMLADKLIVTEIDADFDGGASFPAPDAAHWQE
VSRDAHQAAAPNTFGYAFVVYTRKRD

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=163, Percent_Identity=41.1042944785276, Blast_Score=131, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17505899, Length=183, Percent_Identity=28.9617486338798, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 20290; Mature: 20290

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRATVPVKSAPFRFPSSPTFMTTLTLIVARARNGIIGRDNQLPWKLPEDLAFFKRTTMGA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCC
PIVMGRKTHESIGRPLPGRRNIVVTRDATRRFEGCDTVTSLADALALAARDGVPEAFLIG
CEEECCCCHHHHCCCCCCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEC
GAQLYAEGLMLADKLIVTEIDADFDGGASFPAPDAAHWQEVSRDAHQAAAPNTFGYAFVV
CHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
YTRKRD
EEECCC
>Mature Secondary Structure
MRATVPVKSAPFRFPSSPTFMTTLTLIVARARNGIIGRDNQLPWKLPEDLAFFKRTTMGA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCC
PIVMGRKTHESIGRPLPGRRNIVVTRDATRRFEGCDTVTSLADALALAARDGVPEAFLIG
CEEECCCCHHHHCCCCCCCCCEEEECCHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEC
GAQLYAEGLMLADKLIVTEIDADFDGGASFPAPDAAHWQEVSRDAHQAAAPNTFGYAFVV
CHHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEE
YTRKRD
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]