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The map label for this gene is yafV [C]

Identifier: 107021816

GI number: 107021816

Start: 277033

End: 277860

Strand: Reverse

Name: yafV [C]

Synonym: Bcen_0256

Alternate gene names: 107021816

Gene position: 277860-277033 (Counterclockwise)

Preceding gene: 107021817

Following gene: 107021815

Centisome position: 8.43

GC content: 69.08

Gene sequence:

>828_bases
ATGACCGACCCCGCCCGTTCCGCCACGCCCTTCCAGGTCGCCGCGCTGCAGATGGTGAGCACGCCGGACGTCACCCGCAA
TCTCGCCGAAGCACGCCGCCTGATCGCGGAAGCCGCCGATGAAGGCGCGCAGCTCGTGCTGCTGCCCGAGTATTTCTGCT
TCATGGGCCACCGCGACACCGACAAGCTCGCGCTCGCCGAACCGTACCGGGACGGCCCGATCCAGCAGTTCCTCGCCGAC
GCCGCGCGCCGCCACGGCATCTGGGTGATCGGCGGCACGCTGCCGCTGAAGGCGCCGGAACCCGATCGCGTGCTGAACAC
GACGCTCGTGTTCGATCCGTCCGGCAACGAAGCCGCACGCTACGACAAGATTCACCTGTTCAACTTCGAGAAAGGCGACG
AATCGTTCGACGAGGCGCGCACGATCCGCGCGGGCGACACGGTCGTCGCGTTCGACGCGCCGTTCGGGCAGGTCGGCCTG
TCGGTCTGTTACGATCTCCGCTTTCCGGAGCTGTATCGCCGCATGGGCGACTGCGCGCTGATGGTCGTGCCGTCGGCGTT
TACGTATACGACGGGCCGCGCGCACTGGGAAATGCTGCTGCGCGCGCGGGCCGTCGAGAATCAGTGCTACGTGCTCGCGG
CCGCGCAGGGCGGCAAGCACGAGAACGGCCGGCGCACGTGGGGCCACAGCATGCTGATCGACCCGTGGGGCGAGATCGTC
GCGGTGCGCGACGAGGGCGCCAGCGTCGTGCTCGGCACGCTCGATCCGCAACGCATCGCCGACGTACGCCAGAGCCTGCC
CGCGTGGCGGCACCGCGTGCTGACCTGA

Upstream 100 bases:

>100_bases
AGATGAATCACGGTCCGGCCGATGCCTCGAGCCATTGAGCGCGCATCGACCCGCGCAGCGCGCCGGGCCGCACCCCATTT
ATGACGCAACCGATTCCGCG

Downstream 100 bases:

>100_bases
ATCCGCCGCACCGCTTGAAACGCCGCGCGGCCCACCCATCTGCCCGGCAGCACCCGACAACTTTTTGAGAATCCCGATAT
CCGCATGAACATCATCGAAC

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 274

Protein sequence:

>275_residues
MTDPARSATPFQVAALQMVSTPDVTRNLAEARRLIAEAADEGAQLVLLPEYFCFMGHRDTDKLALAEPYRDGPIQQFLAD
AARRHGIWVIGGTLPLKAPEPDRVLNTTLVFDPSGNEAARYDKIHLFNFEKGDESFDEARTIRAGDTVVAFDAPFGQVGL
SVCYDLRFPELYRRMGDCALMVVPSAFTYTTGRAHWEMLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIV
AVRDEGASVVLGTLDPQRIADVRQSLPAWRHRVLT

Sequences:

>Translated_275_residues
MTDPARSATPFQVAALQMVSTPDVTRNLAEARRLIAEAADEGAQLVLLPEYFCFMGHRDTDKLALAEPYRDGPIQQFLAD
AARRHGIWVIGGTLPLKAPEPDRVLNTTLVFDPSGNEAARYDKIHLFNFEKGDESFDEARTIRAGDTVVAFDAPFGQVGL
SVCYDLRFPELYRRMGDCALMVVPSAFTYTTGRAHWEMLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIV
AVRDEGASVVLGTLDPQRIADVRQSLPAWRHRVLT
>Mature_274_residues
TDPARSATPFQVAALQMVSTPDVTRNLAEARRLIAEAADEGAQLVLLPEYFCFMGHRDTDKLALAEPYRDGPIQQFLADA
ARRHGIWVIGGTLPLKAPEPDRVLNTTLVFDPSGNEAARYDKIHLFNFEKGDESFDEARTIRAGDTVVAFDAPFGQVGLS
VCYDLRFPELYRRMGDCALMVVPSAFTYTTGRAHWEMLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIVA
VRDEGASVVLGTLDPQRIADVRQSLPAWRHRVLT

Specific function: Unknown

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI9910460, Length=268, Percent_Identity=37.3134328358209, Blast_Score=189, Evalue=2e-48,
Organism=Homo sapiens, GI5031947, Length=267, Percent_Identity=37.4531835205993, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI297632348, Length=267, Percent_Identity=37.8277153558052, Blast_Score=182, Evalue=3e-46,
Organism=Homo sapiens, GI297632350, Length=267, Percent_Identity=37.4531835205993, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI297632346, Length=192, Percent_Identity=32.8125, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1786412, Length=230, Percent_Identity=30, Blast_Score=82, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17556280, Length=265, Percent_Identity=40, Blast_Score=197, Evalue=5e-51,
Organism=Saccharomyces cerevisiae, GI6323383, Length=273, Percent_Identity=35.5311355311355, Blast_Score=176, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6322335, Length=296, Percent_Identity=34.1216216216216, Blast_Score=164, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17933642, Length=278, Percent_Identity=35.2517985611511, Blast_Score=177, Evalue=5e-45,
Organism=Drosophila melanogaster, GI21355835, Length=276, Percent_Identity=32.9710144927536, Blast_Score=166, Evalue=1e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010
- InterPro:   IPR001110 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: 3.5.-.- [C]

Molecular weight: Translated: 30583; Mature: 30452

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPARSATPFQVAALQMVSTPDVTRNLAEARRLIAEAADEGAQLVLLPEYFCFMGHRDT
CCCCCCCCCCHHHEEEEHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCC
DKLALAEPYRDGPIQQFLADAARRHGIWVIGGTLPLKAPEPDRVLNTTLVFDPSGNEAAR
CCEEECCCCCCCHHHHHHHHHHHHCCEEEEECEEECCCCCCCCEEEEEEEECCCCCCCCC
YDKIHLFNFEKGDESFDEARTIRAGDTVVAFDAPFGQVGLSVCYDLRFPELYRRMGDCAL
CCEEEEEECCCCCCCHHHHHEEECCCEEEEECCCCHHHHHHHHEECCCHHHHHHHCCEEE
MVVPSAFTYTTGRAHWEMLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIV
EEECCCEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEE
AVRDEGASVVLGTLDPQRIADVRQSLPAWRHRVLT
EEECCCCEEEEECCCHHHHHHHHHHCHHHHHHCCC
>Mature Secondary Structure 
TDPARSATPFQVAALQMVSTPDVTRNLAEARRLIAEAADEGAQLVLLPEYFCFMGHRDT
CCCCCCCCCHHHEEEEHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHCCCCCC
DKLALAEPYRDGPIQQFLADAARRHGIWVIGGTLPLKAPEPDRVLNTTLVFDPSGNEAAR
CCEEECCCCCCCHHHHHHHHHHHHCCEEEEECEEECCCCCCCCEEEEEEEECCCCCCCCC
YDKIHLFNFEKGDESFDEARTIRAGDTVVAFDAPFGQVGLSVCYDLRFPELYRRMGDCAL
CCEEEEEECCCCCCCHHHHHEEECCCEEEEECCCCHHHHHHHHEECCCHHHHHHHCCEEE
MVVPSAFTYTTGRAHWEMLLRARAVENQCYVLAAAQGGKHENGRRTWGHSMLIDPWGEIV
EEECCCEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEECCCCCEE
AVRDEGASVVLGTLDPQRIADVRQSLPAWRHRVLT
EEECCCCEEEEECCCHHHHHHHHHHCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]