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The map label for this gene is mutM

Identifier: 103488662

GI number: 103488662

Start: 3343577

End: 3344389

Strand: Direct

Name: mutM

Synonym: Sala_3187

Alternate gene names: 103488662

Gene position: 3343577-3344389 (Clockwise)

Preceding gene: 103488653

Following gene: 103488663

Centisome position: 99.95

GC content: 67.16

Gene sequence:

>813_bases
ATGCCCGAACTTCCCGAAGTCGAAACCACCGTGCGCGGCCTTGTCCCCTTTCTCGAAGGACAACGGCTGGCCGCCGTCAC
CACCTTCCGCCCCGACCTTCGCCGCCCCTTTCCCGTTGACCTCGCGCAGCGGCTGACGGGTGCGACCGTGACCAGGCTTT
CGCGCCGCGCCAAATATGGCATCGTCTCGACCGACCGCGACGATCATATGATCTTTCACCTCGGCATGTCGGGACGCTGG
CGGACCGAGGGCGGCGAGCCCGGCAAGCACGACCATCTGCTCCTCGAAACCGGCGCGGGGCACCGGCTCTTTCTCCACGA
CCCGCGGCGCTTCGGCTCGATCGATCTGGTCGCGGGCGATCCGCTGGCCAGCTTTGCGGCCTTCGTGACGCTCGGCCCCG
AACCGCTGTCGGACGATTTCGACGCGGCCTTGCTGGCACGGGCGTTCGCGGCGCGCCGGGCGCCGGTGAAGGCGATGCTG
CTCGACCAGAATGTCGTCGCGGGGCTCGGCAACATCTATGTCTGCGAGGCGCTCAACATGGCGCGCATTTCGCCGCTGAT
GCCCGCCGCCGGTGTGCCCAAGGCCAAACTTGCCGCGCTCGTGTCCGCGATCAGGGCGGTGCTCACCGCCGCGATCGCTG
CTGGCGGATCGACGCTGCGCGATTTTTTGAGCCCCGATGGCGACCTTGGTTATTTCGCAAAGGACTGGCGCGTCTATGGC
CGCGAGGGCGAGGCTTGCGAATGCGGCGGCGCGATCGTTCGCGTCGTGCAGTCGGGTCGCTCGACCTTTTACTGCCGCAA
ATGCCAGCGATGA

Upstream 100 bases:

>100_bases
GCCATCGTTTGCGCGCCTTTTACGGGGGAGGTCATCTGGTGCGTCGGCCTTTAGCCAAGCCTTGCCGCGCAAGCAAATGG
CGCGTAGCGGGGCAAGCCTG

Downstream 100 bases:

>100_bases
GGCGGGATATTCGCCGCAAGACCATTGACCAAATCGCCCTTCATCGCTATGCGCGCACCCTTCGAGCAGGGTCCGGACGT
CCGGAACCGGCTGCATCCGG

Product: formamidopyrimidine-DNA glycosylase

Products: NA

Alternate protein names: Fapy-DNA glycosylase; DNA-(apurinic or apyrimidinic site) lyase mutM; AP lyase mutM

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MPELPEVETTVRGLVPFLEGQRLAAVTTFRPDLRRPFPVDLAQRLTGATVTRLSRRAKYGIVSTDRDDHMIFHLGMSGRW
RTEGGEPGKHDHLLLETGAGHRLFLHDPRRFGSIDLVAGDPLASFAAFVTLGPEPLSDDFDAALLARAFAARRAPVKAML
LDQNVVAGLGNIYVCEALNMARISPLMPAAGVPKAKLAALVSAIRAVLTAAIAAGGSTLRDFLSPDGDLGYFAKDWRVYG
REGEACECGGAIVRVVQSGRSTFYCRKCQR

Sequences:

>Translated_270_residues
MPELPEVETTVRGLVPFLEGQRLAAVTTFRPDLRRPFPVDLAQRLTGATVTRLSRRAKYGIVSTDRDDHMIFHLGMSGRW
RTEGGEPGKHDHLLLETGAGHRLFLHDPRRFGSIDLVAGDPLASFAAFVTLGPEPLSDDFDAALLARAFAARRAPVKAML
LDQNVVAGLGNIYVCEALNMARISPLMPAAGVPKAKLAALVSAIRAVLTAAIAAGGSTLRDFLSPDGDLGYFAKDWRVYG
REGEACECGGAIVRVVQSGRSTFYCRKCQR
>Mature_269_residues
PELPEVETTVRGLVPFLEGQRLAAVTTFRPDLRRPFPVDLAQRLTGATVTRLSRRAKYGIVSTDRDDHMIFHLGMSGRWR
TEGGEPGKHDHLLLETGAGHRLFLHDPRRFGSIDLVAGDPLASFAAFVTLGPEPLSDDFDAALLARAFAARRAPVKAMLL
DQNVVAGLGNIYVCEALNMARISPLMPAAGVPKAKLAALVSAIRAVLTAAIAAGGSTLRDFLSPDGDLGYFAKDWRVYGR
EGEACECGGAIVRVVQSGRSTFYCRKCQR

Specific function: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG). Has AP (apurinic/apyr

COG id: COG0266

COG function: function code L; Formamidopyrimidine-DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FPG-type zinc finger

Homologues:

Organism=Escherichia coli, GI1790066, Length=273, Percent_Identity=42.1245421245421, Blast_Score=194, Evalue=5e-51,
Organism=Escherichia coli, GI1786932, Length=276, Percent_Identity=25.7246376811594, Blast_Score=69, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FPG_SPHAL (Q1GN82)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_618223.1
- ProteinModelPortal:   Q1GN82
- SMR:   Q1GN82
- GeneID:   4082404
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_3187
- NMPDR:   fig|317655.9.peg.3035
- HOGENOM:   HBG690070
- OMA:   RMTGQLL
- ProtClustDB:   PRK01103
- BioCyc:   SALA317655:SALA_3187-MONOMER
- BRENDA:   3.2.2.23
- BRENDA:   4.2.99.18
- HAMAP:   MF_00103
- InterPro:   IPR015886
- InterPro:   IPR015887
- InterPro:   IPR000191
- InterPro:   IPR012319
- InterPro:   IPR020629
- InterPro:   IPR010979
- InterPro:   IPR000214
- InterPro:   IPR010663
- SMART:   SM00898
- TIGRFAMs:   TIGR00577

Pfam domain/function: PF01149 Fapy_DNA_glyco; PF06831 H2TH; PF06827 zf-FPG_IleRS; SSF81624 Form_DNAglyc_cat; SSF46946 Ribosomal_H2TH

EC number: =3.2.2.23; =4.2.99.18

Molecular weight: Translated: 29230; Mature: 29099

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: PS51068 FPG_CAT; PS01242 ZF_FPG_1; PS51066 ZF_FPG_2

Important sites: ACT_SITE 2-2 ACT_SITE 3-3 ACT_SITE 58-58 ACT_SITE 260-260 BINDING 90-90 BINDING 109-109 BINDING 152-152

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPELPEVETTVRGLVPFLEGQRLAAVTTFRPDLRRPFPVDLAQRLTGATVTRLSRRAKYG
CCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
IVSTDRDDHMIFHLGMSGRWRTEGGEPGKHDHLLLETGAGHRLFLHDPRRFGSIDLVAGD
CEECCCCCEEEEEECCCCEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCEEEEECC
PLASFAAFVTLGPEPLSDDFDAALLARAFAARRAPVKAMLLDQNVVAGLGNIYVCEALNM
CHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEEECCCH
ARISPLMPAAGVPKAKLAALVSAIRAVLTAAIAAGGSTLRDFLSPDGDLGYFAKDWRVYG
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEECEEEEC
REGEACECGGAIVRVVQSGRSTFYCRKCQR
CCCCCCHHHHHHHHHHHCCCCEEEHHCCCC
>Mature Secondary Structure 
PELPEVETTVRGLVPFLEGQRLAAVTTFRPDLRRPFPVDLAQRLTGATVTRLSRRAKYG
CCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
IVSTDRDDHMIFHLGMSGRWRTEGGEPGKHDHLLLETGAGHRLFLHDPRRFGSIDLVAGD
CEECCCCCEEEEEECCCCEEECCCCCCCCCCEEEEECCCCCEEEEECCCCCCCEEEEECC
PLASFAAFVTLGPEPLSDDFDAALLARAFAARRAPVKAMLLDQNVVAGLGNIYVCEALNM
CHHHHHHHHEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCEEEEECCCH
ARISPLMPAAGVPKAKLAALVSAIRAVLTAAIAAGGSTLRDFLSPDGDLGYFAKDWRVYG
HHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCEEECEEEEC
REGEACECGGAIVRVVQSGRSTFYCRKCQR
CCCCCCHHHHHHHHHHHCCCCEEEHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA