Click here to switch to the map view.

The map label for this gene is gmk

Identifier: 103488630

GI number: 103488630

Start: 3303763

End: 3304425

Strand: Direct

Name: gmk

Synonym: Sala_3155

Alternate gene names: 103488630

Gene position: 3303763-3304425 (Clockwise)

Preceding gene: 103488628

Following gene: 103488635

Centisome position: 98.76

GC content: 65.31

Gene sequence:

>663_bases
ATGGCAGATAGCGTCCACCTCAACCGCCGCGGCGTGTTGTTCGTCCTTTCCTCGCCGTCGGGCGCGGGCAAGACCACCAT
TTCGCGCATGATGCTGGAGGAGGACAAGGACATTGCCCTTTCGGTCTCCGCGACCACGCGACCGCCGCGGCCGGGCGAGA
TCGATGGCGTCCATTATCATTTCGTCGACACCGACACCTTCAAGAAAATGGCCGCCGACGGGGCCTTCCTCGAATGGGCG
CATGTGTTCGGCCACCGCTACGGCACGCCGCGCGCGCCGGTTGAGGAATTGCTCGCCGCGGGCAAGGACGTGCTGTTCGA
CATCGACTGGCAGGGCGCACAGCAACTCTATCAGGAGGCCGGGCCCGACGTCGTGCGCGTTTTCGTGCTGCCGCCGACGA
TGGAGGAACTCGAACGCCGCCTGCGCGCGCGGGGGACCGACAGCGACGAGGTGATCGCCGCGCGCATGGCGCGCGCCGCG
AACGAGATCAGCCACTGGGACGGTTATGACTATGTGCTGATCAACGACCATGTCGGCGAATGTTATGGCGAGGTGATGGC
GATCCTGCGCGCCGAACGGCTGAAGCGCCGCCGCCAGATCGGGCTGATCGGTTTCGCGCGCGACCTGATCCGATCGGTGC
CTGATGCGGACACGAAGCTTTGA

Upstream 100 bases:

>100_bases
CGGCGACCAGCCAGCCCGTTCGCGAAATCCCCCCGACCATCATGCCCCCATGTCCGCGCTTGCCGCTTGACCGGCGTGCC
CTCCCGCGCAATTAGCGCGC

Downstream 100 bases:

>100_bases
TTTGTGATTCCCTCCCCCTCTATGGGGGAGGCAGCGAAACTGGCCTCGCCATCGTCGCCTCCAGCTCCGCGACCATCGCG
GCGCGCAGCGGTTTCGCCGC

Product: guanylate kinase

Products: NA

Alternate protein names: GMP kinase

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MADSVHLNRRGVLFVLSSPSGAGKTTISRMMLEEDKDIALSVSATTRPPRPGEIDGVHYHFVDTDTFKKMAADGAFLEWA
HVFGHRYGTPRAPVEELLAAGKDVLFDIDWQGAQQLYQEAGPDVVRVFVLPPTMEELERRLRARGTDSDEVIAARMARAA
NEISHWDGYDYVLINDHVGECYGEVMAILRAERLKRRRQIGLIGFARDLIRSVPDADTKL

Sequences:

>Translated_220_residues
MADSVHLNRRGVLFVLSSPSGAGKTTISRMMLEEDKDIALSVSATTRPPRPGEIDGVHYHFVDTDTFKKMAADGAFLEWA
HVFGHRYGTPRAPVEELLAAGKDVLFDIDWQGAQQLYQEAGPDVVRVFVLPPTMEELERRLRARGTDSDEVIAARMARAA
NEISHWDGYDYVLINDHVGECYGEVMAILRAERLKRRRQIGLIGFARDLIRSVPDADTKL
>Mature_219_residues
ADSVHLNRRGVLFVLSSPSGAGKTTISRMMLEEDKDIALSVSATTRPPRPGEIDGVHYHFVDTDTFKKMAADGAFLEWAH
VFGHRYGTPRAPVEELLAAGKDVLFDIDWQGAQQLYQEAGPDVVRVFVLPPTMEELERRLRARGTDSDEVIAARMARAAN
EISHWDGYDYVLINDHVGECYGEVMAILRAERLKRRRQIGLIGFARDLIRSVPDADTKL

Specific function: Essential for recycling GMP and indirectly, cGMP

COG id: COG0194

COG function: function code F; Guanylate kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 guanylate kinase-like domain

Homologues:

Organism=Homo sapiens, GI226874833, Length=193, Percent_Identity=37.3056994818653, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI4504221, Length=193, Percent_Identity=37.3056994818653, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI226874831, Length=193, Percent_Identity=37.3056994818653, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI14780902, Length=190, Percent_Identity=25.2631578947368, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1790080, Length=192, Percent_Identity=51.0416666666667, Blast_Score=201, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI193203132, Length=181, Percent_Identity=37.0165745856354, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17508835, Length=181, Percent_Identity=37.0165745856354, Blast_Score=124, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI17557440, Length=182, Percent_Identity=28.021978021978, Blast_Score=70, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71990720, Length=86, Percent_Identity=44.1860465116279, Blast_Score=68, Evalue=5e-12,
Organism=Saccharomyces cerevisiae, GI6320662, Length=182, Percent_Identity=36.8131868131868, Blast_Score=138, Evalue=8e-34,
Organism=Drosophila melanogaster, GI21356141, Length=166, Percent_Identity=36.7469879518072, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): KGUA_SPHAL (Q1GNB4)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_618191.1
- ProteinModelPortal:   Q1GNB4
- SMR:   Q1GNB4
- GeneID:   4082491
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_3155
- NMPDR:   fig|317655.9.peg.3003
- HOGENOM:   HBG671982
- OMA:   DIDWQGT
- ProtClustDB:   PRK00300
- BioCyc:   SALA317655:SALA_3155-MONOMER
- BRENDA:   2.7.4.8
- GO:   GO:0005737
- HAMAP:   MF_00328
- InterPro:   IPR008144
- InterPro:   IPR008145
- InterPro:   IPR020590
- InterPro:   IPR017665
- SMART:   SM00072
- TIGRFAMs:   TIGR03263

Pfam domain/function: PF00625 Guanylate_kin

EC number: =2.7.4.8

Molecular weight: Translated: 24635; Mature: 24504

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS00856 GUANYLATE_KINASE_1; PS50052 GUANYLATE_KINASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSVHLNRRGVLFVLSSPSGAGKTTISRMMLEEDKDIALSVSATTRPPRPGEIDGVHYH
CCCCEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEE
FVDTDTFKKMAADGAFLEWAHVFGHRYGTPRAPVEELLAAGKDVLFDIDWQGAQQLYQEA
EECHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHC
GPDVVRVFVLPPTMEELERRLRARGTDSDEVIAARMARAANEISHWDGYDYVLINDHVGE
CCCEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHH
CYGEVMAILRAERLKRRRQIGLIGFARDLIRSVPDADTKL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ADSVHLNRRGVLFVLSSPSGAGKTTISRMMLEEDKDIALSVSATTRPPRPGEIDGVHYH
CCCEEECCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCEEEE
FVDTDTFKKMAADGAFLEWAHVFGHRYGTPRAPVEELLAAGKDVLFDIDWQGAQQLYQEA
EECHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCEEEECCCHHHHHHHHHC
GPDVVRVFVLPPTMEELERRLRARGTDSDEVIAARMARAANEISHWDGYDYVLINDHVGE
CCCEEEEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHH
CYGEVMAILRAERLKRRRQIGLIGFARDLIRSVPDADTKL
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA