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The map label for this gene is fadE25 [H]

Identifier: 103488553

GI number: 103488553

Start: 3228218

End: 3229399

Strand: Direct

Name: fadE25 [H]

Synonym: Sala_3077

Alternate gene names: 103488553

Gene position: 3228218-3229399 (Clockwise)

Preceding gene: 103488540

Following gene: 103488554

Centisome position: 96.5

GC content: 63.79

Gene sequence:

>1182_bases
ATGAGCGAACTGGACGCGTTCCGCACCGAGGTGCGCGCATGGCTGGCGGCGAATTGCCCCCCCGAAATGCGTGAGCCCGT
GCGCGACGAGGATGATGTCTGCTGGGGCGGGCGCAAATGGGTGTTCAAGAACGACGCCCAGAAGGCGTGGCTCGAAGCCT
GCGTTTCCAAGGGCTATACCGTCCCTGACTGGCCCAAGGCCTATGGCGGCGCGGGCCTCACCCCCGAACGGACCAAGATC
CTCAAACAGGAAATGAAGCGCATCCATGCCCGCTCGCCGCTCGACAGCTTCGGCATCTGGATGCTCGGCCCCGCGCTGCT
CAAATTCGGCACCGAGGAGCAGAAGGTCCATTATCTGAACCAGATCGCGCGCGGCGAAATCCGCTGGTGCCAGGGCTATA
GCGAACCCGGCGCGGGCAGCGATCTCGTCAGCTTGCAGACCTTCGGCGAGGACAAGGGCGATCACTGGGTCGTCAACGGC
TCGAAGATCTGGACCAGCTATGCCGACAAGGCCGACTGGATCTTCTGCCTCGTCCGCACCGACAAGACGAACAAATATCA
GGGCATCACCTTCATGCTTTTCGACATGGAGAGCCCCGGCGTCACCACCAAGCCGATCCTGCTGATCTCCGGCAACTCGC
CCTTCTGCGAGACTTTCTTCGACGATGTGAAGGTGCCCAAGACGCAATATGTGGGCGAGATCAACCGCGGCTGGGACGTC
GCCAAATATCTGCTCGGCCACGAACGCGAGATGATTTCGGGCGGCGGTGCGGGCGGCGACATGGTCAGCGTCGGCGCGCT
CTTCGCGAAATCGCTCGGGCTCAACGACCGCGGCGAACTCGACGACCCCGTCCTGCGCGCCGAAATGGCGGCGTTCGACA
CCGACGTCTTCGCCTATCGCGCGATGGGCGAGCGCTTCATGGACATGTGGAAGACCGGCCGCGCGCATCCGGCCAGCTCG
AACATGATGAAATATGTCGGGACCGAGCTCAACAAGCGTCGGCACGAACTGGTGATGGCGGGCGGCGGCAGCGACGCGCT
CGAATGGGACAGCGCCGAAAGCAGGGGCGGCGCCCGCGCGCGCAGCTGGCTGCGCACCAAGGCCAATTCGATCGAGGGCG
GGACGAGCGAAGTCATGCTCAACGTCATCGCCAAGCGCATCCTGGAATTGCCGGGGGCGTAA

Upstream 100 bases:

>100_bases
ACGCTACGGCGCCGTAGCGGCAGGCGTCGACGCCCAAGTTGACGCTTGCGTAAAGCGGCGAAGCGGACGTAGGTTTGCGC
GCAGAAGAGGAGCCAAAAGG

Downstream 100 bases:

>100_bases
CGACTCGTCGCCCCAGCGAAAGCTGGGGCCGCTATCGGGACAGAGCGGTGTTGGCCGCCAACGATCCCAGCTTTCGCTGG
GATGACGACAAGAGAGATTG

Product: acyl-CoA dehydrogenase-like protein

Products: 2,3-dehydroacyl-CoA; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MSELDAFRTEVRAWLAANCPPEMREPVRDEDDVCWGGRKWVFKNDAQKAWLEACVSKGYTVPDWPKAYGGAGLTPERTKI
LKQEMKRIHARSPLDSFGIWMLGPALLKFGTEEQKVHYLNQIARGEIRWCQGYSEPGAGSDLVSLQTFGEDKGDHWVVNG
SKIWTSYADKADWIFCLVRTDKTNKYQGITFMLFDMESPGVTTKPILLISGNSPFCETFFDDVKVPKTQYVGEINRGWDV
AKYLLGHEREMISGGGAGGDMVSVGALFAKSLGLNDRGELDDPVLRAEMAAFDTDVFAYRAMGERFMDMWKTGRAHPASS
NMMKYVGTELNKRRHELVMAGGGSDALEWDSAESRGGARARSWLRTKANSIEGGTSEVMLNVIAKRILELPGA

Sequences:

>Translated_393_residues
MSELDAFRTEVRAWLAANCPPEMREPVRDEDDVCWGGRKWVFKNDAQKAWLEACVSKGYTVPDWPKAYGGAGLTPERTKI
LKQEMKRIHARSPLDSFGIWMLGPALLKFGTEEQKVHYLNQIARGEIRWCQGYSEPGAGSDLVSLQTFGEDKGDHWVVNG
SKIWTSYADKADWIFCLVRTDKTNKYQGITFMLFDMESPGVTTKPILLISGNSPFCETFFDDVKVPKTQYVGEINRGWDV
AKYLLGHEREMISGGGAGGDMVSVGALFAKSLGLNDRGELDDPVLRAEMAAFDTDVFAYRAMGERFMDMWKTGRAHPASS
NMMKYVGTELNKRRHELVMAGGGSDALEWDSAESRGGARARSWLRTKANSIEGGTSEVMLNVIAKRILELPGA
>Mature_392_residues
SELDAFRTEVRAWLAANCPPEMREPVRDEDDVCWGGRKWVFKNDAQKAWLEACVSKGYTVPDWPKAYGGAGLTPERTKIL
KQEMKRIHARSPLDSFGIWMLGPALLKFGTEEQKVHYLNQIARGEIRWCQGYSEPGAGSDLVSLQTFGEDKGDHWVVNGS
KIWTSYADKADWIFCLVRTDKTNKYQGITFMLFDMESPGVTTKPILLISGNSPFCETFFDDVKVPKTQYVGEINRGWDVA
KYLLGHEREMISGGGAGGDMVSVGALFAKSLGLNDRGELDDPVLRAEMAAFDTDVFAYRAMGERFMDMWKTGRAHPASSN
MMKYVGTELNKRRHELVMAGGGSDALEWDSAESRGGARARSWLRTKANSIEGGTSEVMLNVIAKRILELPGA

Specific function: Catalyzes the reduction of crotonobetainyl-CoA to gamma-butyrobetainyl-CoA. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the acyl-CoA dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI4501859, Length=353, Percent_Identity=26.9121813031161, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI226958412, Length=200, Percent_Identity=33, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI226958414, Length=200, Percent_Identity=33, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI4557233, Length=348, Percent_Identity=27.5862068965517, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI4501857, Length=399, Percent_Identity=22.3057644110276, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI4557231, Length=154, Percent_Identity=30.5194805194805, Blast_Score=81, Evalue=2e-15,
Organism=Homo sapiens, GI187960098, Length=154, Percent_Identity=30.5194805194805, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI4557235, Length=214, Percent_Identity=28.5046728971963, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI76496475, Length=214, Percent_Identity=28.5046728971963, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI21361497, Length=150, Percent_Identity=34, Blast_Score=73, Evalue=5e-13,
Organism=Homo sapiens, GI7656849, Length=245, Percent_Identity=26.530612244898, Blast_Score=73, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17538396, Length=198, Percent_Identity=32.8282828282828, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17508101, Length=403, Percent_Identity=23.0769230769231, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17534899, Length=208, Percent_Identity=30.7692307692308, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17506239, Length=337, Percent_Identity=26.4094955489614, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17569725, Length=208, Percent_Identity=31.25, Blast_Score=92, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17570075, Length=245, Percent_Identity=28.1632653061224, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI32563615, Length=194, Percent_Identity=30.9278350515464, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71990804, Length=201, Percent_Identity=28.8557213930348, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI17505929, Length=179, Percent_Identity=30.1675977653631, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI71982178, Length=199, Percent_Identity=29.1457286432161, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17551932, Length=197, Percent_Identity=28.4263959390863, Blast_Score=81, Evalue=9e-16,
Organism=Drosophila melanogaster, GI24666513, Length=363, Percent_Identity=25.6198347107438, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21356377, Length=204, Percent_Identity=34.3137254901961, Blast_Score=96, Evalue=4e-20,
Organism=Drosophila melanogaster, GI21355753, Length=197, Percent_Identity=34.5177664974619, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24660351, Length=160, Percent_Identity=31.875, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24646207, Length=177, Percent_Identity=31.0734463276836, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281363737, Length=153, Percent_Identity=30.718954248366, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006089
- InterPro:   IPR006092
- InterPro:   IPR006090
- InterPro:   IPR006091
- InterPro:   IPR009075
- InterPro:   IPR013786
- InterPro:   IPR009100 [H]

Pfam domain/function: PF00441 Acyl-CoA_dh_1; PF02770 Acyl-CoA_dh_M; PF02771 Acyl-CoA_dh_N [H]

EC number: 1.3.99.3

Molecular weight: Translated: 43821; Mature: 43690

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELDAFRTEVRAWLAANCPPEMREPVRDEDDVCWGGRKWVFKNDAQKAWLEACVSKGYT
CCHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEECCCEEEEECCHHHHHHHHHHHCCCC
VPDWPKAYGGAGLTPERTKILKQEMKRIHARSPLDSFGIWMLGPALLKFGTEEQKVHYLN
CCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
QIARGEIRWCQGYSEPGAGSDLVSLQTFGEDKGDHWVVNGSKIWTSYADKADWIFCLVRT
HHHCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEECCEEEHHHCCCCCEEEEEEEE
DKTNKYQGITFMLFDMESPGVTTKPILLISGNSPFCETFFDDVKVPKTQYVGEINRGWDV
CCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHCCCCCHHH
AKYLLGHEREMISGGGAGGDMVSVGALFAKSLGLNDRGELDDPVLRAEMAAFDTDVFAYR
HHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AMGERFMDMWKTGRAHPASSNMMKYVGTELNKRRHELVMAGGGSDALEWDSAESRGGARA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCHHH
RSWLRTKANSIEGGTSEVMLNVIAKRILELPGA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SELDAFRTEVRAWLAANCPPEMREPVRDEDDVCWGGRKWVFKNDAQKAWLEACVSKGYT
CHHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCEECCCEEEEECCHHHHHHHHHHHCCCC
VPDWPKAYGGAGLTPERTKILKQEMKRIHARSPLDSFGIWMLGPALLKFGTEEQKVHYLN
CCCCCHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHH
QIARGEIRWCQGYSEPGAGSDLVSLQTFGEDKGDHWVVNGSKIWTSYADKADWIFCLVRT
HHHCCCHHHHCCCCCCCCCCCEEEEEECCCCCCCEEEEECCEEEHHHCCCCCEEEEEEEE
DKTNKYQGITFMLFDMESPGVTTKPILLISGNSPFCETFFDDVKVPKTQYVGEINRGWDV
CCCCCCCCEEEEEEECCCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHCCCCCHHH
AKYLLGHEREMISGGGAGGDMVSVGALFAKSLGLNDRGELDDPVLRAEMAAFDTDVFAYR
HHHHHCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
AMGERFMDMWKTGRAHPASSNMMKYVGTELNKRRHELVMAGGGSDALEWDSAESRGGARA
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCCCCCCCCHHH
RSWLRTKANSIEGGTSEVMLNVIAKRILELPGA
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acyl-CoA; acceptor

Specific reaction: acyl-CoA + acceptor = 2,3-dehydroacyl-CoA + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]