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The map label for this gene is mmsB [H]

Identifier: 103488532

GI number: 103488532

Start: 3206592

End: 3207467

Strand: Direct

Name: mmsB [H]

Synonym: Sala_3056

Alternate gene names: 103488532

Gene position: 3206592-3207467 (Clockwise)

Preceding gene: 103488531

Following gene: 103488533

Centisome position: 95.86

GC content: 66.55

Gene sequence:

>876_bases
ATGAAAATCGCCTTTATCGGACTCGGCAACATGGGCGGCGGGATGGCCGCGAACCTTGCGAAGGCGGGGCATGACGTGCG
CGCTTACGACCTCAGCGAAGAGGCGTTGACGCGCGCAGCCGATGCGGGCTGTTCGCGCGCGGCATCGGCGGCCGAAGCGG
TGACCGGCGCCGAGGCCGTCGTTACCATGCTCCCCGCGGGCACGCATGTCGCGGGTGTTTATGAAAACGACGTCTTTCCG
AACGCTGCGCCGGGCACGCTGCTCCTCGACTGCTCGACGATCGACGTCGCGACCGCGCGCTCGAACATCGAGGCCGCGAC
CGCCAAGGGCCTCGTTGCGGTCGATGCGCCGGTGTCGGGCGGCATCGCCGCCGCCAATGCCGGCACGCTGACCTTCATGG
TCGGCGGCACCGAAGAAGGCTTTGCGCGCGCGCAGCCGATCCTTGCCAAAATGGGCAAGGCGGTGATCCACGCGGGCGAT
GCGGGCGCGGGGCAGGCGGCGAAGATCTGCAACAACATGCTGCTCGGCGCGTCGATGGTCGCAACGTGCGAGACGCTCGC
GCTCGCGCAGAAACTCGGGCTCGATCCGCAAAAATTCTTCGACATCGCTAGCGTCTCGTCGGGGCAATGCTGGTCGCTGA
CCAGCTATGCGCCGCTCCCCGGCGTCGGTCCGGTCACGCCCGCCGACAATGATTACAAGGGCGGGTTTGCCGCGGCGCTG
ATGCTCAAGGATCTGCGTCTCGCGATGGAAGCGGCGGCGAGCGTCAACGCCGACGTGCCGATGGGGACGAAAGCGCGCGA
GCTATACGAAGCCTTTGTCGAAGCCGACAAGGACGGTCGCGACTTTTCGGCGATCATCCGGACATTGCAGGGCTGA

Upstream 100 bases:

>100_bases
GCAGAGCTGCACGTCGTACCCCTCTCCAACTAGGGCTAGGCGCTTCGCGCCAATCCTTCATATCTTCTCCCCTGAAGGGG
AGAGGCTAACGGAGAGACAA

Downstream 100 bases:

>100_bases
TGACCCTCGACTTTGCGGGGCCTCCGGCGCTAAGGCCGCGCGCGAATCAAGAAAAGACAGGATTTCTCCCATGCGTATCG
ACCTCATCCCGGCGGGCGAC

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 291; Mature: 291

Protein sequence:

>291_residues
MKIAFIGLGNMGGGMAANLAKAGHDVRAYDLSEEALTRAADAGCSRAASAAEAVTGAEAVVTMLPAGTHVAGVYENDVFP
NAAPGTLLLDCSTIDVATARSNIEAATAKGLVAVDAPVSGGIAAANAGTLTFMVGGTEEGFARAQPILAKMGKAVIHAGD
AGAGQAAKICNNMLLGASMVATCETLALAQKLGLDPQKFFDIASVSSGQCWSLTSYAPLPGVGPVTPADNDYKGGFAAAL
MLKDLRLAMEAAASVNADVPMGTKARELYEAFVEADKDGRDFSAIIRTLQG

Sequences:

>Translated_291_residues
MKIAFIGLGNMGGGMAANLAKAGHDVRAYDLSEEALTRAADAGCSRAASAAEAVTGAEAVVTMLPAGTHVAGVYENDVFP
NAAPGTLLLDCSTIDVATARSNIEAATAKGLVAVDAPVSGGIAAANAGTLTFMVGGTEEGFARAQPILAKMGKAVIHAGD
AGAGQAAKICNNMLLGASMVATCETLALAQKLGLDPQKFFDIASVSSGQCWSLTSYAPLPGVGPVTPADNDYKGGFAAAL
MLKDLRLAMEAAASVNADVPMGTKARELYEAFVEADKDGRDFSAIIRTLQG
>Mature_291_residues
MKIAFIGLGNMGGGMAANLAKAGHDVRAYDLSEEALTRAADAGCSRAASAAEAVTGAEAVVTMLPAGTHVAGVYENDVFP
NAAPGTLLLDCSTIDVATARSNIEAATAKGLVAVDAPVSGGIAAANAGTLTFMVGGTEEGFARAQPILAKMGKAVIHAGD
AGAGQAAKICNNMLLGASMVATCETLALAQKLGLDPQKFFDIASVSSGQCWSLTSYAPLPGVGPVTPADNDYKGGFAAAL
MLKDLRLAMEAAASVNADVPMGTKARELYEAFVEADKDGRDFSAIIRTLQG

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=290, Percent_Identity=42.7586206896552, Blast_Score=236, Evalue=1e-62,
Organism=Homo sapiens, GI40556376, Length=288, Percent_Identity=27.7777777777778, Blast_Score=101, Evalue=9e-22,
Organism=Escherichia coli, GI145693186, Length=274, Percent_Identity=35.4014598540146, Blast_Score=149, Evalue=2e-37,
Organism=Escherichia coli, GI1790315, Length=295, Percent_Identity=35.2542372881356, Blast_Score=143, Evalue=1e-35,
Organism=Escherichia coli, GI1786719, Length=295, Percent_Identity=29.8305084745763, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1789092, Length=295, Percent_Identity=27.7966101694915, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI17557316, Length=290, Percent_Identity=41.3793103448276, Blast_Score=219, Evalue=9e-58,
Organism=Drosophila melanogaster, GI24655230, Length=288, Percent_Identity=40.625, Blast_Score=225, Evalue=3e-59,
Organism=Drosophila melanogaster, GI19922568, Length=288, Percent_Identity=40.625, Blast_Score=225, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 29357; Mature: 29357

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAFIGLGNMGGGMAANLAKAGHDVRAYDLSEEALTRAADAGCSRAASAAEAVTGAEAV
CEEEEEECCCCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHH
VTMLPAGTHVAGVYENDVFPNAAPGTLLLDCSTIDVATARSNIEAATAKGLVAVDAPVSG
HHCCCCCCEEEEEECCCCCCCCCCCEEEEECCCEEEHHHHCCCHHHHHCCEEEECCCCCC
GIAAANAGTLTFMVGGTEEGFARAQPILAKMGKAVIHAGDAGAGQAAKICNNMLLGASMV
CEEECCCCEEEEEECCCCCCHHHHHHHHHHHCHHEEECCCCCCCHHHHHHHHHHHHHHHH
ATCETLALAQKLGLDPQKFFDIASVSSGQCWSLTSYAPLPGVGPVTPADNDYKGGFAAAL
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
MLKDLRLAMEAAASVNADVPMGTKARELYEAFVEADKDGRDFSAIIRTLQG
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC
>Mature Secondary Structure
MKIAFIGLGNMGGGMAANLAKAGHDVRAYDLSEEALTRAADAGCSRAASAAEAVTGAEAV
CEEEEEECCCCCCCHHHHHHHCCCCEEEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHH
VTMLPAGTHVAGVYENDVFPNAAPGTLLLDCSTIDVATARSNIEAATAKGLVAVDAPVSG
HHCCCCCCEEEEEECCCCCCCCCCCEEEEECCCEEEHHHHCCCHHHHHCCEEEECCCCCC
GIAAANAGTLTFMVGGTEEGFARAQPILAKMGKAVIHAGDAGAGQAAKICNNMLLGASMV
CEEECCCCEEEEEECCCCCCHHHHHHHHHHHCHHEEECCCCCCCHHHHHHHHHHHHHHHH
ATCETLALAQKLGLDPQKFFDIASVSSGQCWSLTSYAPLPGVGPVTPADNDYKGGFAAAL
HHHHHHHHHHHHCCCHHHHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
MLKDLRLAMEAAASVNADVPMGTKARELYEAFVEADKDGRDFSAIIRTLQG
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339433; 10984043 [H]