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The map label for this gene is gyrB [H]

Identifier: 103488449

GI number: 103488449

Start: 3115117

End: 3117693

Strand: Direct

Name: gyrB [H]

Synonym: Sala_2972

Alternate gene names: 103488449

Gene position: 3115117-3117693 (Clockwise)

Preceding gene: 103488447

Following gene: 103488450

Centisome position: 93.12

GC content: 65.23

Gene sequence:

>2577_bases
ATGACAGACACCCCCGAGAACAGCGCGCCCGAAGGCGCCGCAAAACAGCCCAACGCCAACGCCTATGGCGCCGATTCGAT
CAAGGTATTGAAGGGGCTCGATGCCGTGCGCAAACGGCCGGGCATGTATATCGGCGACACCGACGACGGGTCGGGGCTGC
ATCATATGGTGTTCGAGGTCAGCGACAATGCGATCGACGAGGCGCTTGCCGGTCATTGCGACCTCATACTCATCACATTG
AACAGCGACGGGTCGGTCAGCGTCGAGGATAATGGCCGCGGCATCCCCACCGACATCCACGCCGAAGAGGGCGTGTCGGC
CGCCGAAGTCATCATGACCCAGCTCCACGCGGGCGGGAAGTTCGAGAATACCAGCGACGACAATGCCTACAAGGTGTCGG
GCGGCCTCCACGGCGTCGGCGTCAGCGTCGTCAACGCCCTGTCCGAATGGCTCGAACTCACCATCTGGCGCGACGGGCAG
GAACATTGGATGCGGTTCGAGCATGGCGACGCCGTCGCCCCGCTCCGCGTCAACGGCCCCGCCCCGGCGGGCAAAAAGGG
CACGCGCGTCACCTTCCAGGCGTCGACCGACACGTTCAAGAATGTCACCGAGTTCGATTTCGAAAAGCTCGAGCATCGCT
ACCGCGAGCTGGCGTTCCTCAATTCTGGGGTGCGGATCAAGCTCGTCGACGCGCGCCACGCCGAACATGTCAGCCACGAC
CTCTTCTATGAGGGCGGCATCGCGGCGTTCGTCAAATATCTCGACCGCAACAAGACCCCGCTGCTCCCCGATCCGATCGC
GATCAGCAGCGAGCGCGACGGCATCGGCATCGACGTCGCATTGGAATGGAACGACAGCTATTATGAAAATGTCCTCTGCT
TCACGAACAATATCCCGCAGCGCGACGGCGGCACGCACCTCGCCGCCTTCCGCGCCGCGCTGACGCGCACCTTGAACGGC
TATGGCGAAAAGTCGGGACTGCTGAAGAAGGAGAAGGTGTCGCTCACCGGCGAGGATATGCGCGAAGGGCTGACCGCGAT
CGTCTCGGTCAAGCTGCCCGACCCCAAGTTCAGCTCGCAGACCAAGGACAAGCTCGTCTCCTCCGAAGTCCGCCAGCCGC
TCGAAAGCCTGATGGCCGACCGGATGACCGAATGGCTCGAGGAAAATCCTGCCTATGCGAAGGCGGTGATCCAGAAGGTC
GTCGATGCCGCCGCGGCGCGCGAGGCCGCGAAAAAGGCGCGCGAGCTCACCCGGCGCAAGGGCGCGATGGACATCGCCTC
GCTCCCCGGCAAGCTCGCCGACTGCCAGGAACGCGACCCCGCGAAATGCGAGCTTTTCCTTGTCGAGGGTGACTCGGCAG
GCGGCAGTGCGAAGCAGGGCCGCGACCGGCATGTGCAGGCGATCCTGCCCTTGAAGGGCAAGATCCTGAACGTCGAGCGC
GCGCGTTTCGACCGCATCATTTCGTCGAAGGAGGTCGGTACGCTGATCCAGGCGCTCGGCACCGGCATCCGCGACGAGTT
CAACATTGAAAAACTGCGCTATCACAAGATTGTGATCATGACCGACGCCGACGTCGACGGCGCGCATATCCGCACGCTTC
TGCTCACCTTCTTCTACCGCCAGATGCCCGAAATCATCGAGAAGGGGCATCTCTACATCGCGCAGCCGCCGCTCTATAAG
GTCGCGAAGGGGCGGAGCGAGGTTTACCTGAAAGACGACAGCGCGCTCGAAAACTACCTCGTCGACGGCGGGATCGATGC
GCTGATGCTCGAAACCACCGGCGGCGCGCGGTCGGGCGCCGATTTGCGCGGACTGATCGAACATGGGCGCCGTTTGCGCG
CGCTGATGCGCTATGTCCCGCGCGGCCATAATTATGGGCTGGTCGAGGCGCTGGCGCTGAACGGCGCCCTCGATCCGGCG
CTCGACAGCGCGGGCCGCACCGCCGCCGCGGTCCGCGCCGCCGAATGGCTCAACGCCGCCGAGCGCGCGCTGACCGGCGG
CGCCGAGGCGAAATGGACGATCACGGCCGCGGGCGGCGGCTACACGCTCGAAAAGCGCTGGCGCGGGGTCAGCGACCATC
ATGCGATCGACGCGGCCTTCCTCGCGAGCCAGGAGGCGCGTCGGCTGCACACGCTTGCCGCGGAACAGGCCGAAACCTAT
GCGCACCCGGCGCGGCTGGTAAAGGGCAGCAGCGCCGCGGCGCTCGCCGCCGACGCCGCCGCGCTCGCCGCGACCGAGGC
AGCCGAGGACGATGCCGAAGCGAGCGACGGCGACCTGCCCGCCGCGACCGCCCGCAAGGTCACGCCGATCACGCGTCCGT
CCGAACTGCTCGAGGCGATCTTCGCGCACAGCCGCAAGGGCCTCGCGATCAGCCGCTACAAGGGGCTGGGCGAGATGAAT
GCCGAACAGCTTTGGGAAACGACGCTCGATCCCTCGAACCGTTCGCTGCTGCGCGTCGAGGCCGAACAGGCCGACATTGC
GCACGAAATCTTCGAACGATTGATGGGCGACGAGGTCGAACCGCGCCGCGATTTCATCCAGACCAACGCGCTGTCGGTGG
CGAATCTCGACGTCTGA

Upstream 100 bases:

>100_bases
AAACTAGCGGTCGCCGCGCGGGGGTGCAACGCCCGTCGCGCGAAGGCCGAGAGCGGCATTTTCGCTTGGGTTTTATGCGC
GCCGACCCTATATAATCGAC

Downstream 100 bases:

>100_bases
ACGCACATCCGGCCGCAAATAGGGTTCGATTGGACGCGCGGCGGCTTGCCTCGGCACGCCGCCGCTGCTTTGGGCGCTTC
CTCTGACAAGAGGATGTGTC

Product: DNA gyrase, B subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 858; Mature: 857

Protein sequence:

>858_residues
MTDTPENSAPEGAAKQPNANAYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVSDNAIDEALAGHCDLILITL
NSDGSVSVEDNGRGIPTDIHAEEGVSAAEVIMTQLHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLELTIWRDGQ
EHWMRFEHGDAVAPLRVNGPAPAGKKGTRVTFQASTDTFKNVTEFDFEKLEHRYRELAFLNSGVRIKLVDARHAEHVSHD
LFYEGGIAAFVKYLDRNKTPLLPDPIAISSERDGIGIDVALEWNDSYYENVLCFTNNIPQRDGGTHLAAFRAALTRTLNG
YGEKSGLLKKEKVSLTGEDMREGLTAIVSVKLPDPKFSSQTKDKLVSSEVRQPLESLMADRMTEWLEENPAYAKAVIQKV
VDAAAAREAAKKARELTRRKGAMDIASLPGKLADCQERDPAKCELFLVEGDSAGGSAKQGRDRHVQAILPLKGKILNVER
ARFDRIISSKEVGTLIQALGTGIRDEFNIEKLRYHKIVIMTDADVDGAHIRTLLLTFFYRQMPEIIEKGHLYIAQPPLYK
VAKGRSEVYLKDDSALENYLVDGGIDALMLETTGGARSGADLRGLIEHGRRLRALMRYVPRGHNYGLVEALALNGALDPA
LDSAGRTAAAVRAAEWLNAAERALTGGAEAKWTITAAGGGYTLEKRWRGVSDHHAIDAAFLASQEARRLHTLAAEQAETY
AHPARLVKGSSAAALAADAAALAATEAAEDDAEASDGDLPAATARKVTPITRPSELLEAIFAHSRKGLAISRYKGLGEMN
AEQLWETTLDPSNRSLLRVEAEQADIAHEIFERLMGDEVEPRRDFIQTNALSVANLDV

Sequences:

>Translated_858_residues
MTDTPENSAPEGAAKQPNANAYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVSDNAIDEALAGHCDLILITL
NSDGSVSVEDNGRGIPTDIHAEEGVSAAEVIMTQLHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLELTIWRDGQ
EHWMRFEHGDAVAPLRVNGPAPAGKKGTRVTFQASTDTFKNVTEFDFEKLEHRYRELAFLNSGVRIKLVDARHAEHVSHD
LFYEGGIAAFVKYLDRNKTPLLPDPIAISSERDGIGIDVALEWNDSYYENVLCFTNNIPQRDGGTHLAAFRAALTRTLNG
YGEKSGLLKKEKVSLTGEDMREGLTAIVSVKLPDPKFSSQTKDKLVSSEVRQPLESLMADRMTEWLEENPAYAKAVIQKV
VDAAAAREAAKKARELTRRKGAMDIASLPGKLADCQERDPAKCELFLVEGDSAGGSAKQGRDRHVQAILPLKGKILNVER
ARFDRIISSKEVGTLIQALGTGIRDEFNIEKLRYHKIVIMTDADVDGAHIRTLLLTFFYRQMPEIIEKGHLYIAQPPLYK
VAKGRSEVYLKDDSALENYLVDGGIDALMLETTGGARSGADLRGLIEHGRRLRALMRYVPRGHNYGLVEALALNGALDPA
LDSAGRTAAAVRAAEWLNAAERALTGGAEAKWTITAAGGGYTLEKRWRGVSDHHAIDAAFLASQEARRLHTLAAEQAETY
AHPARLVKGSSAAALAADAAALAATEAAEDDAEASDGDLPAATARKVTPITRPSELLEAIFAHSRKGLAISRYKGLGEMN
AEQLWETTLDPSNRSLLRVEAEQADIAHEIFERLMGDEVEPRRDFIQTNALSVANLDV
>Mature_857_residues
TDTPENSAPEGAAKQPNANAYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEVSDNAIDEALAGHCDLILITLN
SDGSVSVEDNGRGIPTDIHAEEGVSAAEVIMTQLHAGGKFENTSDDNAYKVSGGLHGVGVSVVNALSEWLELTIWRDGQE
HWMRFEHGDAVAPLRVNGPAPAGKKGTRVTFQASTDTFKNVTEFDFEKLEHRYRELAFLNSGVRIKLVDARHAEHVSHDL
FYEGGIAAFVKYLDRNKTPLLPDPIAISSERDGIGIDVALEWNDSYYENVLCFTNNIPQRDGGTHLAAFRAALTRTLNGY
GEKSGLLKKEKVSLTGEDMREGLTAIVSVKLPDPKFSSQTKDKLVSSEVRQPLESLMADRMTEWLEENPAYAKAVIQKVV
DAAAAREAAKKARELTRRKGAMDIASLPGKLADCQERDPAKCELFLVEGDSAGGSAKQGRDRHVQAILPLKGKILNVERA
RFDRIISSKEVGTLIQALGTGIRDEFNIEKLRYHKIVIMTDADVDGAHIRTLLLTFFYRQMPEIIEKGHLYIAQPPLYKV
AKGRSEVYLKDDSALENYLVDGGIDALMLETTGGARSGADLRGLIEHGRRLRALMRYVPRGHNYGLVEALALNGALDPAL
DSAGRTAAAVRAAEWLNAAERALTGGAEAKWTITAAGGGYTLEKRWRGVSDHHAIDAAFLASQEARRLHTLAAEQAETYA
HPARLVKGSSAAALAADAAALAATEAAEDDAEASDGDLPAATARKVTPITRPSELLEAIFAHSRKGLAISRYKGLGEMNA
EQLWETTLDPSNRSLLRVEAEQADIAHEIFERLMGDEVEPRRDFIQTNALSVANLDV

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=567, Percent_Identity=24.1622574955908, Blast_Score=129, Evalue=2e-29,
Organism=Homo sapiens, GI19913406, Length=569, Percent_Identity=25.1318101933216, Blast_Score=125, Evalue=2e-28,
Organism=Escherichia coli, GI48994957, Length=852, Percent_Identity=55.9859154929578, Blast_Score=879, Evalue=0.0,
Organism=Escherichia coli, GI1789408, Length=554, Percent_Identity=40.6137184115523, Blast_Score=371, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI17535065, Length=551, Percent_Identity=25.4083484573503, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI212645845, Length=515, Percent_Identity=24.4660194174757, Blast_Score=115, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI212645657, Length=173, Percent_Identity=30.635838150289, Blast_Score=79, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6324241, Length=598, Percent_Identity=25.0836120401338, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI17136538, Length=545, Percent_Identity=26.2385321100917, Blast_Score=130, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 93329; Mature: 93198

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTPENSAPEGAAKQPNANAYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEV
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEEEE
SDNAIDEALAGHCDLILITLNSDGSVSVEDNGRGIPTDIHAEEGVSAAEVIMTQLHAGGK
CCHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCC
FENTSDDNAYKVSGGLHGVGVSVVNALSEWLELTIWRDGQEHWMRFEHGDAVAPLRVNGP
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHEEEEEECCHHHHHEECCCCEEEEEEECCC
APAGKKGTRVTFQASTDTFKNVTEFDFEKLEHRYRELAFLNSGVRIKLVDARHAEHVSHD
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHH
LFYEGGIAAFVKYLDRNKTPLLPDPIAISSERDGIGIDVALEWNDSYYENVLCFTNNIPQ
HHHCCCHHHHHHHHHCCCCCCCCCCCEECCCCCCCEEEEEEECCCCHHCCEEEEECCCCC
RDGGTHLAAFRAALTRTLNGYGEKSGLLKKEKVSLTGEDMREGLTAIVSVKLPDPKFSSQ
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHCCCEEEEEEECCCCCCCCH
TKDKLVSSEVRQPLESLMADRMTEWLEENPAYAKAVIQKVVDAAAAREAAKKARELTRRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAMDIASLPGKLADCQERDPAKCELFLVEGDSAGGSAKQGRDRHVQAILPLKGKILNVER
CCCCHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEHHH
ARFDRIISSKEVGTLIQALGTGIRDEFNIEKLRYHKIVIMTDADVDGAHIRTLLLTFFYR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHH
QMPEIIEKGHLYIAQPPLYKVAKGRSEVYLKDDSALENYLVDGGIDALMLETTGGARSGA
HHHHHHHCCCEEEECCCHHHHHCCCCEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCC
DLRGLIEHGRRLRALMRYVPRGHNYGLVEALALNGALDPALDSAGRTAAAVRAAEWLNAA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHH
ERALTGGAEAKWTITAAGGGYTLEKRWRGVSDHHAIDAAFLASQEARRLHTLAAEQAETY
HHHHCCCCCCEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHPARLVKGSSAAALAADAAALAATEAAEDDAEASDGDLPAATARKVTPITRPSELLEAI
HCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
FAHSRKGLAISRYKGLGEMNAEQLWETTLDPSNRSLLRVEAEQADIAHEIFERLMGDEVE
HHCCCCCCEEHHHCCCCCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCC
PRRDFIQTNALSVANLDV
CHHHHHHHCCEEEEECCC
>Mature Secondary Structure 
TDTPENSAPEGAAKQPNANAYGADSIKVLKGLDAVRKRPGMYIGDTDDGSGLHHMVFEV
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEECCCCCCCCCEEEEEEE
SDNAIDEALAGHCDLILITLNSDGSVSVEDNGRGIPTDIHAEEGVSAAEVIMTQLHAGGK
CCHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCHHCCCHHHHHHHHHHHCCCC
FENTSDDNAYKVSGGLHGVGVSVVNALSEWLELTIWRDGQEHWMRFEHGDAVAPLRVNGP
CCCCCCCCCEEECCCCCHHHHHHHHHHHHHHEEEEEECCHHHHHEECCCCEEEEEEECCC
APAGKKGTRVTFQASTDTFKNVTEFDFEKLEHRYRELAFLNSGVRIKLVDARHAEHVSHD
CCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHH
LFYEGGIAAFVKYLDRNKTPLLPDPIAISSERDGIGIDVALEWNDSYYENVLCFTNNIPQ
HHHCCCHHHHHHHHHCCCCCCCCCCCEECCCCCCCEEEEEEECCCCHHCCEEEEECCCCC
RDGGTHLAAFRAALTRTLNGYGEKSGLLKKEKVSLTGEDMREGLTAIVSVKLPDPKFSSQ
CCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHCCCCHHHHHCCCEEEEEEECCCCCCCCH
TKDKLVSSEVRQPLESLMADRMTEWLEENPAYAKAVIQKVVDAAAAREAAKKARELTRRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GAMDIASLPGKLADCQERDPAKCELFLVEGDSAGGSAKQGRDRHVQAILPLKGKILNVER
CCCCHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEHHH
ARFDRIISSKEVGTLIQALGTGIRDEFNIEKLRYHKIVIMTDADVDGAHIRTLLLTFFYR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHH
QMPEIIEKGHLYIAQPPLYKVAKGRSEVYLKDDSALENYLVDGGIDALMLETTGGARSGA
HHHHHHHCCCEEEECCCHHHHHCCCCEEEECCCHHHHHHHHCCCCCEEEEEECCCCCCCC
DLRGLIEHGRRLRALMRYVPRGHNYGLVEALALNGALDPALDSAGRTAAAVRAAEWLNAA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHH
ERALTGGAEAKWTITAAGGGYTLEKRWRGVSDHHAIDAAFLASQEARRLHTLAAEQAETY
HHHHCCCCCCEEEEEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHPARLVKGSSAAALAADAAALAATEAAEDDAEASDGDLPAATARKVTPITRPSELLEAI
HCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
FAHSRKGLAISRYKGLGEMNAEQLWETTLDPSNRSLLRVEAEQADIAHEIFERLMGDEVE
HHCCCCCCEEHHHCCCCCCCHHHHHHHCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCC
PRRDFIQTNALSVANLDV
CHHHHHHHCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8226640 [H]