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The map label for this gene is hemC

Identifier: 103488353

GI number: 103488353

Start: 3027464

End: 3028411

Strand: Direct

Name: hemC

Synonym: Sala_2876

Alternate gene names: 103488353

Gene position: 3027464-3028411 (Clockwise)

Preceding gene: 103488349

Following gene: 103488354

Centisome position: 90.5

GC content: 72.47

Gene sequence:

>948_bases
ATGAACGAGCTTGCGACCCCCGACAACCCGCTTCGCATCGGCACGCGCGCCTCGCCGCTGGCGATGGCGCAGGCGCATAT
GGCGGCGGCCGCTCTGATCGCGGCGCACGGGCTCGCCATGGCGGCGCTCGAAATCGTGCCGATGACCGCGACGGGCGACC
GGATTCAGGATCGCGCGCTCGCCGAGGTTGGCGGCAAGGCGCTGTGGACGCGCGAGCTTGACGCCGCGCTCGATGCGGGG
ACGATCGACGTCGCCGTCCATTCGCTCAAGGATGTCGAAACGCTGCGCGACGCGCGCTTTTTCCTGGGCGCGATGCTCGA
GCGCGCCGATCCGCGCGACCGGCTGGTGGTGCGCGAGGGGATTGCCGCGCAAAAGATTTCCGAGCTGCCGCACGGCGCCC
GGCTGGGGACGAGCAGCCCGCGGCGCGCGGCGCAGGTGCGGCGGCTGCGCCCCGATCTCGACACGGTGTTGCTGCGCGGC
AATGTCGCGACGCGGCTCGCCAAGCTCGCCGCGGGCGAGGCCGACGCGACCCTGCTCGCCGCGGCGGGGCTCGAACGGCT
GGGGATGCACGACATCGGCGCTGTGCAGGGCACGGAACTGCTGCTGCCGGCGGCGTCGCAGGGCGCGATCGGCATCGAAT
GCCGCGCCGACGACGCGGCAACCATCGCGCTGCTCGCCGCGATCGACCATGCGCCGACGCATCGCGCCGTCGCCGCCGAA
CGCGCCCTGCTCGCGGTGCTCGGCGGCGATTGCCGCTCGCCGGTCGCGGCCTATGCGGAGTGGCAGGCGGACGGCGTTCT
CCGCCTCGATGCAGAGATTTTCTCCGAAGATGGCGCCGATCATGTCGCGGGGCATGTCATCGTCACCGACGCCGGGGTCG
TCGCGGCGCTGGGGCATCGGCTGCTGGCCGAAGCGCCGCCGAGCATCCGGCGGTTGTTCGCGACATGA

Upstream 100 bases:

>100_bases
CGTCGCAGCTCGATTCGAGGCCGAGGATCAGGGTCATATTGTCGCGCGTTCCTTTACCCGCCCCTTTATCGGAAGGGCAA
GCCGCGCTAGCAGCAGCCGG

Downstream 100 bases:

>100_bases
GCGACGGCGCGCCGCTGATCGTCACGCGCCCCGAACCGGGCAATGCGGCGACGGTCGCGCGTGCGCGTGCGCTGGGTTTT
GATGTTCATGCGATGCCGCT

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MNELATPDNPLRIGTRASPLAMAQAHMAAAALIAAHGLAMAALEIVPMTATGDRIQDRALAEVGGKALWTRELDAALDAG
TIDVAVHSLKDVETLRDARFFLGAMLERADPRDRLVVREGIAAQKISELPHGARLGTSSPRRAAQVRRLRPDLDTVLLRG
NVATRLAKLAAGEADATLLAAAGLERLGMHDIGAVQGTELLLPAASQGAIGIECRADDAATIALLAAIDHAPTHRAVAAE
RALLAVLGGDCRSPVAAYAEWQADGVLRLDAEIFSEDGADHVAGHVIVTDAGVVAALGHRLLAEAPPSIRRLFAT

Sequences:

>Translated_315_residues
MNELATPDNPLRIGTRASPLAMAQAHMAAAALIAAHGLAMAALEIVPMTATGDRIQDRALAEVGGKALWTRELDAALDAG
TIDVAVHSLKDVETLRDARFFLGAMLERADPRDRLVVREGIAAQKISELPHGARLGTSSPRRAAQVRRLRPDLDTVLLRG
NVATRLAKLAAGEADATLLAAAGLERLGMHDIGAVQGTELLLPAASQGAIGIECRADDAATIALLAAIDHAPTHRAVAAE
RALLAVLGGDCRSPVAAYAEWQADGVLRLDAEIFSEDGADHVAGHVIVTDAGVVAALGHRLLAEAPPSIRRLFAT
>Mature_315_residues
MNELATPDNPLRIGTRASPLAMAQAHMAAAALIAAHGLAMAALEIVPMTATGDRIQDRALAEVGGKALWTRELDAALDAG
TIDVAVHSLKDVETLRDARFFLGAMLERADPRDRLVVREGIAAQKISELPHGARLGTSSPRRAAQVRRLRPDLDTVLLRG
NVATRLAKLAAGEADATLLAAAGLERLGMHDIGAVQGTELLLPAASQGAIGIECRADDAATIALLAAIDHAPTHRAVAAE
RALLAVLGGDCRSPVAAYAEWQADGVLRLDAEIFSEDGADHVAGHVIVTDAGVVAALGHRLLAEAPPSIRRLFAT

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI66933009, Length=275, Percent_Identity=40, Blast_Score=180, Evalue=1e-45,
Organism=Homo sapiens, GI20149500, Length=275, Percent_Identity=40, Blast_Score=180, Evalue=1e-45,
Organism=Escherichia coli, GI48994974, Length=280, Percent_Identity=47.1428571428571, Blast_Score=207, Evalue=7e-55,
Organism=Saccharomyces cerevisiae, GI6319996, Length=261, Percent_Identity=34.8659003831418, Blast_Score=142, Evalue=5e-35,
Organism=Drosophila melanogaster, GI20130425, Length=287, Percent_Identity=39.0243902439024, Blast_Score=180, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_SPHAL (Q1GP41)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_617914.1
- ProteinModelPortal:   Q1GP41
- SMR:   Q1GP41
- GeneID:   4080669
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_2876
- NMPDR:   fig|317655.9.peg.2738
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- BioCyc:   SALA317655:SALA_2876-MONOMER
- BRENDA:   2.5.1.61
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 32849; Mature: 32849

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELATPDNPLRIGTRASPLAMAQAHMAAAALIAAHGLAMAALEIVPMTATGDRIQDRAL
CCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHH
AEVGGKALWTRELDAALDAGTIDVAVHSLKDVETLRDARFFLGAMLERADPRDRLVVREG
HHHCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
IAAQKISELPHGARLGTSSPRRAAQVRRLRPDLDTVLLRGNVATRLAKLAAGEADATLLA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHEEECCHHHHHHHHHHCCCCCHHHHH
AAGLERLGMHDIGAVQGTELLLPAASQGAIGIECRADDAATIALLAAIDHAPTHRAVAAE
HHHHHHCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCCCHHHHHHH
RALLAVLGGDCRSPVAAYAEWQADGVLRLDAEIFSEDGADHVAGHVIVTDAGVVAALGHR
HHHHHHHCCCCCCCHHHHHHHCCCCEEEECHHHHCCCCCCHHHCEEEEECCHHHHHHHHH
LLAEAPPSIRRLFAT
HHHHCCHHHHHHHCC
>Mature Secondary Structure
MNELATPDNPLRIGTRASPLAMAQAHMAAAALIAAHGLAMAALEIVPMTATGDRIQDRAL
CCCCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHH
AEVGGKALWTRELDAALDAGTIDVAVHSLKDVETLRDARFFLGAMLERADPRDRLVVREG
HHHCCCCEEHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHC
IAAQKISELPHGARLGTSSPRRAAQVRRLRPDLDTVLLRGNVATRLAKLAAGEADATLLA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCHHHEEECCHHHHHHHHHHCCCCCHHHHH
AAGLERLGMHDIGAVQGTELLLPAASQGAIGIECRADDAATIALLAAIDHAPTHRAVAAE
HHHHHHCCCCCCCCCCCCEEEEECCCCCCEEEEECCCCHHHHHHHHHHCCCCCHHHHHHH
RALLAVLGGDCRSPVAAYAEWQADGVLRLDAEIFSEDGADHVAGHVIVTDAGVVAALGHR
HHHHHHHCCCCCCCHHHHHHHCCCCEEEECHHHHCCCCCCHHHCEEEEECCHHHHHHHHH
LLAEAPPSIRRLFAT
HHHHCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA