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The map label for this gene is rho [H]

Identifier: 103488322

GI number: 103488322

Start: 2996954

End: 2998210

Strand: Direct

Name: rho [H]

Synonym: Sala_2845

Alternate gene names: 103488322

Gene position: 2996954-2998210 (Clockwise)

Preceding gene: 103488321

Following gene: 103488323

Centisome position: 89.59

GC content: 60.62

Gene sequence:

>1257_bases
ATGCACTTGAAAGAACTCAAAACAAAATCGCCCGCGCAGCTCGTCGAAATGGCCGAGGAACTGGGCGTCGAGGGCGCATC
GACGATGCGCAAGCAGGATCTGATGTTCTCGATCCTGAAGGAGCTCGCCGAAGAGGGCGAGGAAATCATCGGGTCGGGGA
CGATCGAGGTACTGCCCGACAGCTTCGGGTTCCTGCGCTCGTCCGAGGCCAATTATCTCGCCGGTCCCGACGACATCTAT
GTCTCGCCGAACCAGGTCCGCAAATATGGCCTGCGTACCGGCGACACCGTCGAGGGCGAGATTCGTGCGCCGCGCGATGG
CGAACGCTATTTCGCGCTCACCAAGCTGATCAGCGTCAATTTCGACGATCCCGACGTCGTGCGCCACCGCGTCAATTTCG
ATAACCTCACACCGCTCTATCCGAACCAGAAGCTGTCGCTCGACACCGTCGATCCGACGGTCAAGGACAAGTCGGCGCGC
GTCATCGACCTTGTCAGCCCGCAGGGCAAGGGGCAGCGCGCGCTGATCGTCGCGCCGCCGCGCACCGGCAAGACGGTGCT
CTTGCAGAATATCGCCAAGGCGATCACCGACAACCACCCGGAAGTTTACCTCATCGTCCTCCTCGTCGACGAACGGCCCG
AGGAAGTCACCGATATGCAGCGCAGCGTGAAGGGCGAGGTCGTTTCCTCCACCTTCGACGAACCCGCGACGCGCCACGTC
CAGGTCGCCGAAATGGTGATCGAAAAGGCCAAGCGTCTCGTCGAGCACAAGAAGGATGTCGTCATCCTGCTCGATTCGAT
CACGCGCCTCGGTCGTGCCTACAACACCGTCGTCCCTTCGTCGGGCAAGGTGCTGACCGGCGGCGTCGACGCCAATGCGC
TGCAGCGGCCGAAGCGTTTCTTCGGCGCCGCGCGCAACATCGAAGAGGGCGGTTCGCTGTCGATCATCGCCACGGCGCTG
ATCGATACCGGCAGCCGCATGGACGAGGTCATCTTCGAAGAGTTCAAGGGCACGGGTAACAGCGAAATCGTGCTCGACCG
CAAGGTTGCCGACAAGCGCATCTTCCCGGCGCTCGACGTCGGCAAGTCGGGCACGCGCAAGGAAGAATTGCTCGTTGAAA
AGGACAAGCTCTCGAAAATGTGGGTGCTGCGCCGCATCCTCATGCAGATGGGCACCGTCGACGCAATGGAGTTCCTGCTC
GACAAGATCAAGGATTCGAAGACAAACGAGGATTTCTTCGATTCGATGAACCAATAG

Upstream 100 bases:

>100_bases
CCGATGCTATCGTGATCGGCCGTCCCCCGGCCGGATAGGCACCCGGCGGTGCAACGAATTAAGTCCGATTATCTCCCGAA
TATTGATCCGGAATCCATCC

Downstream 100 bases:

>100_bases
GCACGGGGCGGGTTAAGTGATCATGCTGGATATTTTGATGCAGGCCGGCGTGCACGCCGGCGGATTTGGCGGACCCGCGG
GCATGTGGGACGCGATCGTC

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 418; Mature: 418

Protein sequence:

>418_residues
MHLKELKTKSPAQLVEMAEELGVEGASTMRKQDLMFSILKELAEEGEEIIGSGTIEVLPDSFGFLRSSEANYLAGPDDIY
VSPNQVRKYGLRTGDTVEGEIRAPRDGERYFALTKLISVNFDDPDVVRHRVNFDNLTPLYPNQKLSLDTVDPTVKDKSAR
VIDLVSPQGKGQRALIVAPPRTGKTVLLQNIAKAITDNHPEVYLIVLLVDERPEEVTDMQRSVKGEVVSSTFDEPATRHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIATAL
IDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPALDVGKSGTRKEELLVEKDKLSKMWVLRRILMQMGTVDAMEFLL
DKIKDSKTNEDFFDSMNQ

Sequences:

>Translated_418_residues
MHLKELKTKSPAQLVEMAEELGVEGASTMRKQDLMFSILKELAEEGEEIIGSGTIEVLPDSFGFLRSSEANYLAGPDDIY
VSPNQVRKYGLRTGDTVEGEIRAPRDGERYFALTKLISVNFDDPDVVRHRVNFDNLTPLYPNQKLSLDTVDPTVKDKSAR
VIDLVSPQGKGQRALIVAPPRTGKTVLLQNIAKAITDNHPEVYLIVLLVDERPEEVTDMQRSVKGEVVSSTFDEPATRHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIATAL
IDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPALDVGKSGTRKEELLVEKDKLSKMWVLRRILMQMGTVDAMEFLL
DKIKDSKTNEDFFDSMNQ
>Mature_418_residues
MHLKELKTKSPAQLVEMAEELGVEGASTMRKQDLMFSILKELAEEGEEIIGSGTIEVLPDSFGFLRSSEANYLAGPDDIY
VSPNQVRKYGLRTGDTVEGEIRAPRDGERYFALTKLISVNFDDPDVVRHRVNFDNLTPLYPNQKLSLDTVDPTVKDKSAR
VIDLVSPQGKGQRALIVAPPRTGKTVLLQNIAKAITDNHPEVYLIVLLVDERPEEVTDMQRSVKGEVVSSTFDEPATRHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFGAARNIEEGGSLSIIATAL
IDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPALDVGKSGTRKEELLVEKDKLSKMWVLRRILMQMGTVDAMEFLL
DKIKDSKTNEDFFDSMNQ

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=418, Percent_Identity=66.0287081339713, Blast_Score=597, Evalue=1e-172,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 46547; Mature: 46547

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLKELKTKSPAQLVEMAEELGVEGASTMRKQDLMFSILKELAEEGEEIIGSGTIEVLPD
CCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
SFGFLRSSEANYLAGPDDIYVSPNQVRKYGLRTGDTVEGEIRAPRDGERYFALTKLISVN
CCCHHCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCEECCCCCCHHEEEEHHEECC
FDDPDVVRHRVNFDNLTPLYPNQKLSLDTVDPTVKDKSARVIDLVSPQGKGQRALIVAPP
CCCHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEECCCCCCCEEEEEECC
RTGKTVLLQNIAKAITDNHPEVYLIVLLVDERPEEVTDMQRSVKGEVVSSTFDEPATRHV
CCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHCCCCHHHHH
QVAEMVIEKAKRLVEHKKDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHH
FGAARNIEEGGSLSIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPALDV
HHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCCCC
GKSGTRKEELLVEKDKLSKMWVLRRILMQMGTVDAMEFLLDKIKDSKTNEDFFDSMNQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHCCC
>Mature Secondary Structure
MHLKELKTKSPAQLVEMAEELGVEGASTMRKQDLMFSILKELAEEGEEIIGSGTIEVLPD
CCHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCC
SFGFLRSSEANYLAGPDDIYVSPNQVRKYGLRTGDTVEGEIRAPRDGERYFALTKLISVN
CCCHHCCCCCCCCCCCCCEEECHHHHHHHCCCCCCCCCCCEECCCCCCHHEEEEHHEECC
FDDPDVVRHRVNFDNLTPLYPNQKLSLDTVDPTVKDKSARVIDLVSPQGKGQRALIVAPP
CCCHHHHHHHCCCCCCCCCCCCCCEEECCCCCCCCCCCCCEEEEECCCCCCCEEEEEECC
RTGKTVLLQNIAKAITDNHPEVYLIVLLVDERPEEVTDMQRSVKGEVVSSTFDEPATRHV
CCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHCCCCHHHHH
QVAEMVIEKAKRLVEHKKDVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHHHH
FGAARNIEEGGSLSIIATALIDTGSRMDEVIFEEFKGTGNSEIVLDRKVADKRIFPALDV
HHHHCCCCCCCCEEEEHHHHHHCCCHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCCCC
GKSGTRKEELLVEKDKLSKMWVLRRILMQMGTVDAMEFLLDKIKDSKTNEDFFDSMNQ
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606169 [H]