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The map label for this gene is xthA [H]

Identifier: 103488148

GI number: 103488148

Start: 2817401

End: 2818195

Strand: Direct

Name: xthA [H]

Synonym: Sala_2671

Alternate gene names: 103488148

Gene position: 2817401-2818195 (Clockwise)

Preceding gene: 103488147

Following gene: 103488149

Centisome position: 84.22

GC content: 63.65

Gene sequence:

>795_bases
ATGACTCGCACCAGCATCGCTTCGTGGAACATCAACAGCGTCCGCGCCCGCATCGGCATTGTCGAGAAGTTCCTTCGCGA
AGAAGCGCCCGACATCCTCTGCCTGCAGGAAACCAAGGTCGAATGCGGCCTGTTCCCGAAAGCGATGTTCGAGGCACTGG
GTTACGGGCACATCGTCACCCATGGCCAGCGGATGCATCACGGGGTCGCGATCGTCAGCCGCCTGCCCCTTGCCGAGGTG
CGCAAATATGACTGGCAGGCGAATGGCGAGGCGCGCCATATCGGCGTCACGCTGCCGTCGGGGGTGCGGCTCGACAATGT
CTATATTCCCGCCGGCGGCGATATTCCTGACCGTGAAATCAATCCCAAGTTCGGCCAGAAGCTCGATTTTCTGGCGCGCA
TGACCGACTGGTCGGGTGGGCTGAACGGAACGCCCACGGTGCTGACCGGCGACTTCAACGTCGCCCCGCTCGCAAGCGAT
GTGTGGAACCACAAGGCGCTGCTCGATGTCGTCAGCCATACGCCGATAGAGGTCGAAACGCTCGCCCGCTTGCAGGCGGC
GTCGGACTGGGTCGACCTCGGGCGCCATTTCATCGCGGCACCGGCGCCGCTTTATACCTGGTGGAGTTACCGCGCGAAGG
ATTGGGAGGCGTCGAACCGCGGCCGCCGCCTCGACCATATGTGGGTGACGCCCGATCTCAAGGACAAGGCGGTCGCGCAC
CGGATCGTCCAGCCGGCGCGGAGCTGGGAGCGGCCATCGGACCATGTCCCGCTCATCACCGAGTTTGCCTTTTGA

Upstream 100 bases:

>100_bases
GTTCCACCGCCCCTGGGACGGGGGTTTAACGTATCCGGTGCTTTTTGCCGCTGGCGCAATCGCATTGCGAACGGCACCAC
GCACCGATAGAGCCACCCGC

Downstream 100 bases:

>100_bases
GCGCGGCGGCACAGGATCGCGGCGCCCGGCGGGCGGCGCGGGCGATCGACGCGCTGCGCCGCGGGTGGCCGCTGCGCGTC
ACCGGCGCCGACGGCTCGCT

Product: exodeoxyribonuclease III (xth)

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MTRTSIASWNINSVRARIGIVEKFLREEAPDILCLQETKVECGLFPKAMFEALGYGHIVTHGQRMHHGVAIVSRLPLAEV
RKYDWQANGEARHIGVTLPSGVRLDNVYIPAGGDIPDREINPKFGQKLDFLARMTDWSGGLNGTPTVLTGDFNVAPLASD
VWNHKALLDVVSHTPIEVETLARLQAASDWVDLGRHFIAAPAPLYTWWSYRAKDWEASNRGRRLDHMWVTPDLKDKAVAH
RIVQPARSWERPSDHVPLITEFAF

Sequences:

>Translated_264_residues
MTRTSIASWNINSVRARIGIVEKFLREEAPDILCLQETKVECGLFPKAMFEALGYGHIVTHGQRMHHGVAIVSRLPLAEV
RKYDWQANGEARHIGVTLPSGVRLDNVYIPAGGDIPDREINPKFGQKLDFLARMTDWSGGLNGTPTVLTGDFNVAPLASD
VWNHKALLDVVSHTPIEVETLARLQAASDWVDLGRHFIAAPAPLYTWWSYRAKDWEASNRGRRLDHMWVTPDLKDKAVAH
RIVQPARSWERPSDHVPLITEFAF
>Mature_263_residues
TRTSIASWNINSVRARIGIVEKFLREEAPDILCLQETKVECGLFPKAMFEALGYGHIVTHGQRMHHGVAIVSRLPLAEVR
KYDWQANGEARHIGVTLPSGVRLDNVYIPAGGDIPDREINPKFGQKLDFLARMTDWSGGLNGTPTVLTGDFNVAPLASDV
WNHKALLDVVSHTPIEVETLARLQAASDWVDLGRHFIAAPAPLYTWWSYRAKDWEASNRGRRLDHMWVTPDLKDKAVAHR
IVQPARSWERPSDHVPLITEFAF

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=267, Percent_Identity=29.9625468164794, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI18375503, Length=267, Percent_Identity=29.9625468164794, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI18375501, Length=267, Percent_Identity=29.9625468164794, Blast_Score=79, Evalue=5e-15,
Organism=Escherichia coli, GI1788046, Length=271, Percent_Identity=31.7343173431734, Blast_Score=107, Evalue=7e-25,
Organism=Drosophila melanogaster, GI221330655, Length=271, Percent_Identity=29.520295202952, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17136678, Length=272, Percent_Identity=29.4117647058824, Blast_Score=65, Evalue=3e-11,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29715; Mature: 29584

Theoretical pI: Translated: 7.41; Mature: 7.41

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTSIASWNINSVRARIGIVEKFLREEAPDILCLQETKVECGLFPKAMFEALGYGHIVT
CCCCCEECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCEEE
HGQRMHHGVAIVSRLPLAEVRKYDWQANGEARHIGVTLPSGVRLDNVYIPAGGDIPDREI
CCHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCC
NPKFGQKLDFLARMTDWSGGLNGTPTVLTGDFNVAPLASDVWNHKALLDVVSHTPIEVET
CCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHH
LARLQAASDWVDLGRHFIAAPAPLYTWWSYRAKDWEASNRGRRLDHMWVTPDLKDKAVAH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHH
RIVQPARSWERPSDHVPLITEFAF
HHHHHHHHCCCCCCCCCEEEECCC
>Mature Secondary Structure 
TRTSIASWNINSVRARIGIVEKFLREEAPDILCLQETKVECGLFPKAMFEALGYGHIVT
CCCCEECCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHCCCCCEEE
HGQRMHHGVAIVSRLPLAEVRKYDWQANGEARHIGVTLPSGVRLDNVYIPAGGDIPDREI
CCHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCCCC
NPKFGQKLDFLARMTDWSGGLNGTPTVLTGDFNVAPLASDVWNHKALLDVVSHTPIEVET
CCHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHH
LARLQAASDWVDLGRHFIAAPAPLYTWWSYRAKDWEASNRGRRLDHMWVTPDLKDKAVAH
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHH
RIVQPARSWERPSDHVPLITEFAF
HHHHHHHHCCCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]