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The map label for this gene is bcr [H]

Identifier: 103487866

GI number: 103487866

Start: 2514466

End: 2515710

Strand: Reverse

Name: bcr [H]

Synonym: Sala_2385

Alternate gene names: 103487866

Gene position: 2515710-2514466 (Counterclockwise)

Preceding gene: 103487867

Following gene: 103487860

Centisome position: 75.2

GC content: 65.22

Gene sequence:

>1245_bases
ATGCACAAGGCGACCGACTCTCCCCGACGGAGCCTGCCCGGCGACCGTGAAATGGTTGTGATGATGGCGATGGTGATGGC
GCTCAACGCGCTCGCCATCGATTCGATGTTGCCCGCGCTCCCTGCGATCGGCGAAGCGCTGGGGGTCGCCGTCGCCAACG
ACCGCCAATATGTCATTTCCATCTATCTGCTCGGCATCGGCTTCGGATCGCTGATCTATGGTCCGCTCTCCGACCGCTTC
GGGCGCAAGGGTGTGCTTGTGCCTGCGCTCTTCGCCTATCTCGCGCTCTCGATCGGCTGCGGACTGGCGACGAGTTTTGA
AATGCTGCTCGCGCTGCGGTTCGTGCACGGGCTGGTCAGCGCCGCGCTCGGCGTGATCGTCGTCGCGGTGATCCGCGACC
TGTTCGTGGGCGATGCGATGGCGAAGCGTTTGTCGCTGATCTTCCTCGTCTTCATGATCGTCCCGATCATCGCCCCCACG
CTCGGCGCCGGGGTGGCCGCGCTCGCCGGCTGGCGGGCGATCTTCGTCGTGCTCGCCGTAATGTCGGTCGCGATGCTGCT
CTGGCTCCGCCGCCTTCCCGAAACGCTCGATGCGGCCGACGTGCGCCCGCTCGACCTCAAGACGATGGCGGCAGGCTGGG
CCACGGTGACGCGGCATCGCCGCGCCGCGGGCTATATGATCGCGTCGGGCACGATGCAGGGCGCGCTCTATGGCTATCTG
AACAGCAGCGAGCAGATCATCGGCGAGGTGTTCGACGCGCGCAGCCTGTTTCCACTGATCTTTGCCTGCGTCGCCATCGG
CATCGCGATCGCCAATTTTTCGAACGCCGCGATCGTCGAACGCTTCGGTGCGCGGCGCGTCTCGCAGTCGGCGGTCTTCG
CCTTCATGGCGACCTCGATCCTCCAGATCGTCGCGGCGCTGAGCGGCGCCGAGACCTTGGGGATGTTCACCGCGCTGATG
ATGGTCAATGTCGGCCTGATCGGCTTTATCGGCAGCAATTTCGGGTCGATCGCGATGGAGGATTTCGGCCATATGGCGGG
CGTTGCCTCCTCCTATCAGAGCTTTGCCAAGACGCTGCTCGCGGCGGTGGTCGGCGCGGCCATCGGCCAGCAATATGACG
GTACGACGCTGCCGCTCGCTTATGCTTTTCTGATGTGTGCGATCGTCGGGCTGGCGCTGGTGCTGTGGGCCGAGCGCGGC
AGGCTGTTCACCCGGCCGGGCACGGCGCCGAAAAGCCCCTTCTGA

Upstream 100 bases:

>100_bases
GGTCGCTTTCGGACATCGCCCCGCGCACCCCTTCAATTGCATAGCTTTTCCTCGTCGAGGGGCTATCCAAAGCGGCGGCG
CGGGCGCATAGGCTGCTGCG

Downstream 100 bases:

>100_bases
CATTGCGGGACTGTCGACTTCGGGGTGAGATCCGGTCTTCCATTACCGTCATCCCGGCCTTCGCGGGCATGACGATGACG
TGGACATCCGCAACCGCCCT

Product: Bcr/CflA subfamily drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MHKATDSPRRSLPGDREMVVMMAMVMALNALAIDSMLPALPAIGEALGVAVANDRQYVISIYLLGIGFGSLIYGPLSDRF
GRKGVLVPALFAYLALSIGCGLATSFEMLLALRFVHGLVSAALGVIVVAVIRDLFVGDAMAKRLSLIFLVFMIVPIIAPT
LGAGVAALAGWRAIFVVLAVMSVAMLLWLRRLPETLDAADVRPLDLKTMAAGWATVTRHRRAAGYMIASGTMQGALYGYL
NSSEQIIGEVFDARSLFPLIFACVAIGIAIANFSNAAIVERFGARRVSQSAVFAFMATSILQIVAALSGAETLGMFTALM
MVNVGLIGFIGSNFGSIAMEDFGHMAGVASSYQSFAKTLLAAVVGAAIGQQYDGTTLPLAYAFLMCAIVGLALVLWAERG
RLFTRPGTAPKSPF

Sequences:

>Translated_414_residues
MHKATDSPRRSLPGDREMVVMMAMVMALNALAIDSMLPALPAIGEALGVAVANDRQYVISIYLLGIGFGSLIYGPLSDRF
GRKGVLVPALFAYLALSIGCGLATSFEMLLALRFVHGLVSAALGVIVVAVIRDLFVGDAMAKRLSLIFLVFMIVPIIAPT
LGAGVAALAGWRAIFVVLAVMSVAMLLWLRRLPETLDAADVRPLDLKTMAAGWATVTRHRRAAGYMIASGTMQGALYGYL
NSSEQIIGEVFDARSLFPLIFACVAIGIAIANFSNAAIVERFGARRVSQSAVFAFMATSILQIVAALSGAETLGMFTALM
MVNVGLIGFIGSNFGSIAMEDFGHMAGVASSYQSFAKTLLAAVVGAAIGQQYDGTTLPLAYAFLMCAIVGLALVLWAERG
RLFTRPGTAPKSPF
>Mature_414_residues
MHKATDSPRRSLPGDREMVVMMAMVMALNALAIDSMLPALPAIGEALGVAVANDRQYVISIYLLGIGFGSLIYGPLSDRF
GRKGVLVPALFAYLALSIGCGLATSFEMLLALRFVHGLVSAALGVIVVAVIRDLFVGDAMAKRLSLIFLVFMIVPIIAPT
LGAGVAALAGWRAIFVVLAVMSVAMLLWLRRLPETLDAADVRPLDLKTMAAGWATVTRHRRAAGYMIASGTMQGALYGYL
NSSEQIIGEVFDARSLFPLIFACVAIGIAIANFSNAAIVERFGARRVSQSAVFAFMATSILQIVAALSGAETLGMFTALM
MVNVGLIGFIGSNFGSIAMEDFGHMAGVASSYQSFAKTLLAAVVGAAIGQQYDGTTLPLAYAFLMCAIVGLALVLWAERG
RLFTRPGTAPKSPF

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=390, Percent_Identity=30, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI1790146, Length=397, Percent_Identity=27.455919395466, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI48994889, Length=346, Percent_Identity=24.8554913294798, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1787065, Length=234, Percent_Identity=25.6410256410256, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319517, Length=147, Percent_Identity=29.2517006802721, Blast_Score=67, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43733; Mature: 43733

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKATDSPRRSLPGDREMVVMMAMVMALNALAIDSMLPALPAIGEALGVAVANDRQYVIS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHH
IYLLGIGFGSLIYGPLSDRFGRKGVLVPALFAYLALSIGCGLATSFEMLLALRFVHGLVS
HHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AALGVIVVAVIRDLFVGDAMAKRLSLIFLVFMIVPIIAPTLGAGVAALAGWRAIFVVLAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSVAMLLWLRRLPETLDAADVRPLDLKTMAAGWATVTRHRRAAGYMIASGTMQGALYGYL
HHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHC
NSSEQIIGEVFDARSLFPLIFACVAIGIAIANFSNAAIVERFGARRVSQSAVFAFMATSI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LQIVAALSGAETLGMFTALMMVNVGLIGFIGSNFGSIAMEDFGHMAGVASSYQSFAKTLL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AAVVGAAIGQQYDGTTLPLAYAFLMCAIVGLALVLWAERGRLFTRPGTAPKSPF
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC
>Mature Secondary Structure
MHKATDSPRRSLPGDREMVVMMAMVMALNALAIDSMLPALPAIGEALGVAVANDRQYVIS
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHHH
IYLLGIGFGSLIYGPLSDRFGRKGVLVPALFAYLALSIGCGLATSFEMLLALRFVHGLVS
HHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AALGVIVVAVIRDLFVGDAMAKRLSLIFLVFMIVPIIAPTLGAGVAALAGWRAIFVVLAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MSVAMLLWLRRLPETLDAADVRPLDLKTMAAGWATVTRHRRAAGYMIASGTMQGALYGYL
HHHHHHHHHHHCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEECCCHHHHHHHHC
NSSEQIIGEVFDARSLFPLIFACVAIGIAIANFSNAAIVERFGARRVSQSAVFAFMATSI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LQIVAALSGAETLGMFTALMMVNVGLIGFIGSNFGSIAMEDFGHMAGVASSYQSFAKTLL
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AAVVGAAIGQQYDGTTLPLAYAFLMCAIVGLALVLWAERGRLFTRPGTAPKSPF
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]