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The map label for this gene is alkJ [H]

Identifier: 103487812

GI number: 103487812

Start: 2459232

End: 2460818

Strand: Reverse

Name: alkJ [H]

Synonym: Sala_2331

Alternate gene names: 103487812

Gene position: 2460818-2459232 (Counterclockwise)

Preceding gene: 103487813

Following gene: 103487810

Centisome position: 73.56

GC content: 66.6

Gene sequence:

>1587_bases
ATGGATCAGTTCGACATCATCGTCATCGGCGGCGGCAGCGCGGGGAGCGCGGCGGCCGGGCGGCTCGCCGAGGACGGGGC
GCGCACCGTCTGTTTGGTCGAAGCGGGCGGGACGAACGACATCGTGCGGGTGAAGACACCGGGTTTCATGCCCTTCATCC
CCAAATCGTCGAACTGGCGATATGACACCGTGCCGCAACAGGGACTGAACGGCCGCATCGGATATCAGCCGCGCGGGCGC
GGGCTGGGCGGGTCGAGCGCGATCAACGCGATGGTCTATATCCGGGGGCACGCCTTCGATTACGACCAGTGGGCGGCGCT
GGGCGCGACCGGCTGGAGCTATGCCGACGTGCTGCCTTATTTCAAGCGCAGCGAGGGCAATGAGCGCGGCGGTGACGAGT
TTCACGGCGGGGACGGGCCGCTGAATGTGATGGACCAGCGCTGGCCCAATGTGACGAGTCGACGCTTCGTCGAGAGCGCG
ACGGCGCTGCAATTGCCGCGCACTGCTGATTTCAACGGCCCTGACAATGAAGGCTTCGGCCTCTATCAGGTGACGCAGAA
AGGCGGCGAGCGCTGGTCGGCGGCGCGCGCCTATGTCGAGCCGCTGCGCGGGCGATCGAACTTCGACATCCGCACCGGCG
CGCTGGTCGAGAAGATTTTGATCGAGGAGGGGCGCGCGGTCGGTGTCACGATCCGCTGCGGGCGCCGCCGCGAGACGCTG
CGCGCACGGGGTGGGGTCGTGTTGTCGGCGGGGGCGTTCGGCAGTCCGCAGATATTGATGCTGTCGGGGATCGGGCCCGG
CGCGCATTTGCAGGAGATGGGGATTGCCGTCGCGCGCGACCATGCCGGGGTCGGCGACAATCTGCAGGACCATATCGATT
ATGTGTCGAGCTGGGAAACGCGCTCGACCGATCCCTTCGGCGACAGTTTCGGTGGCACCTGGCGGATGGTGAAGGCGATC
GTCGAGCATCGCCGCCGCCGGACGGGGATCATGACGACCTGTTTCGCCGAAGCGGGGGGATTCTGGAAATCGCGCCCCGA
CCTGCCTGCGCCCGACGTGCAGTATCATTTCGTGCCCGCGATGCTCGAGGATCATGGCCGCACCAAGGTCAAGGGGCACG
GCTTTTCGTGCCACGCCTGCGTGCTGCGGCCTGAAAGCAGAGGCACGGTGCGGCTGGCGTCCTCCGATGCCGCGGCGGCA
CCGACGATCGACCCCGGTTTTTTGACCGACGAGCGCGACATGGCGACGCTTCGCGCCGGGGTGCGGATGATGCACCGCAT
CGTCGCGGCGCCGCCGCTCGCCGATTATGCGGGGGTCGACCGCCATCCGGTGAACCTCGATGACGATGCCGCGCTCGACG
CGCTGATCCGCAGCCGCGCCGACACCGTCTATCATCCCGTCGGCACGTGCCGGATGGGCAGCGATGCCGATGCGGTGGTC
GATCCGACACTGAAGCTCAACGGCATCGACGGGCTGTGGGTTGCCGATGCGAGCATCATGCCACGACTGGTCAGCGGCAA
CACCAACGCGCCGAGCATCATGATCGGCGAAAGGGCAGCGGATTTCGTGAAGGCGGCTTTGAGTTAA

Upstream 100 bases:

>100_bases
CCTTCGCGGGGGCGACGTATAGGCTTGCCACGCCGCTTAATGACATTCATGTAAGTCGCGATTGACCACCGAACCGGTGG
CAAGGGAGAGCGGGCGCATC

Downstream 100 bases:

>100_bases
TTTGTGTCGTCATCCCGGCGAAGGCCGGGATGACGACGGTTATAAACAGGCTTCCGCCAACACGCGCCCGCGCCGCGTCA
GATCGTATCGGCTCGCCGGG

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 528

Protein sequence:

>528_residues
MDQFDIIVIGGGSAGSAAAGRLAEDGARTVCLVEAGGTNDIVRVKTPGFMPFIPKSSNWRYDTVPQQGLNGRIGYQPRGR
GLGGSSAINAMVYIRGHAFDYDQWAALGATGWSYADVLPYFKRSEGNERGGDEFHGGDGPLNVMDQRWPNVTSRRFVESA
TALQLPRTADFNGPDNEGFGLYQVTQKGGERWSAARAYVEPLRGRSNFDIRTGALVEKILIEEGRAVGVTIRCGRRRETL
RARGGVVLSAGAFGSPQILMLSGIGPGAHLQEMGIAVARDHAGVGDNLQDHIDYVSSWETRSTDPFGDSFGGTWRMVKAI
VEHRRRRTGIMTTCFAEAGGFWKSRPDLPAPDVQYHFVPAMLEDHGRTKVKGHGFSCHACVLRPESRGTVRLASSDAAAA
PTIDPGFLTDERDMATLRAGVRMMHRIVAAPPLADYAGVDRHPVNLDDDAALDALIRSRADTVYHPVGTCRMGSDADAVV
DPTLKLNGIDGLWVADASIMPRLVSGNTNAPSIMIGERAADFVKAALS

Sequences:

>Translated_528_residues
MDQFDIIVIGGGSAGSAAAGRLAEDGARTVCLVEAGGTNDIVRVKTPGFMPFIPKSSNWRYDTVPQQGLNGRIGYQPRGR
GLGGSSAINAMVYIRGHAFDYDQWAALGATGWSYADVLPYFKRSEGNERGGDEFHGGDGPLNVMDQRWPNVTSRRFVESA
TALQLPRTADFNGPDNEGFGLYQVTQKGGERWSAARAYVEPLRGRSNFDIRTGALVEKILIEEGRAVGVTIRCGRRRETL
RARGGVVLSAGAFGSPQILMLSGIGPGAHLQEMGIAVARDHAGVGDNLQDHIDYVSSWETRSTDPFGDSFGGTWRMVKAI
VEHRRRRTGIMTTCFAEAGGFWKSRPDLPAPDVQYHFVPAMLEDHGRTKVKGHGFSCHACVLRPESRGTVRLASSDAAAA
PTIDPGFLTDERDMATLRAGVRMMHRIVAAPPLADYAGVDRHPVNLDDDAALDALIRSRADTVYHPVGTCRMGSDADAVV
DPTLKLNGIDGLWVADASIMPRLVSGNTNAPSIMIGERAADFVKAALS
>Mature_528_residues
MDQFDIIVIGGGSAGSAAAGRLAEDGARTVCLVEAGGTNDIVRVKTPGFMPFIPKSSNWRYDTVPQQGLNGRIGYQPRGR
GLGGSSAINAMVYIRGHAFDYDQWAALGATGWSYADVLPYFKRSEGNERGGDEFHGGDGPLNVMDQRWPNVTSRRFVESA
TALQLPRTADFNGPDNEGFGLYQVTQKGGERWSAARAYVEPLRGRSNFDIRTGALVEKILIEEGRAVGVTIRCGRRRETL
RARGGVVLSAGAFGSPQILMLSGIGPGAHLQEMGIAVARDHAGVGDNLQDHIDYVSSWETRSTDPFGDSFGGTWRMVKAI
VEHRRRRTGIMTTCFAEAGGFWKSRPDLPAPDVQYHFVPAMLEDHGRTKVKGHGFSCHACVLRPESRGTVRLASSDAAAA
PTIDPGFLTDERDMATLRAGVRMMHRIVAAPPLADYAGVDRHPVNLDDDAALDALIRSRADTVYHPVGTCRMGSDADAVV
DPTLKLNGIDGLWVADASIMPRLVSGNTNAPSIMIGERAADFVKAALS

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=536, Percent_Identity=41.2313432835821, Blast_Score=395, Evalue=1e-110,
Organism=Escherichia coli, GI1786503, Length=533, Percent_Identity=39.9624765478424, Blast_Score=367, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17532301, Length=545, Percent_Identity=37.4311926605505, Blast_Score=370, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24642055, Length=585, Percent_Identity=35.042735042735, Blast_Score=306, Evalue=2e-83,
Organism=Drosophila melanogaster, GI24642042, Length=567, Percent_Identity=35.8024691358025, Blast_Score=305, Evalue=6e-83,
Organism=Drosophila melanogaster, GI45549471, Length=565, Percent_Identity=34.5132743362832, Blast_Score=302, Evalue=5e-82,
Organism=Drosophila melanogaster, GI45551458, Length=565, Percent_Identity=34.5132743362832, Blast_Score=301, Evalue=5e-82,
Organism=Drosophila melanogaster, GI24642059, Length=585, Percent_Identity=35.3846153846154, Blast_Score=287, Evalue=1e-77,
Organism=Drosophila melanogaster, GI24642039, Length=567, Percent_Identity=35.4497354497354, Blast_Score=285, Evalue=6e-77,
Organism=Drosophila melanogaster, GI17137792, Length=559, Percent_Identity=35.778175313059, Blast_Score=285, Evalue=6e-77,
Organism=Drosophila melanogaster, GI24642048, Length=583, Percent_Identity=34.4768439108062, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24642035, Length=583, Percent_Identity=34.3053173241852, Blast_Score=271, Evalue=9e-73,
Organism=Drosophila melanogaster, GI18859995, Length=565, Percent_Identity=34.3362831858407, Blast_Score=263, Evalue=2e-70,
Organism=Drosophila melanogaster, GI24642037, Length=574, Percent_Identity=33.6236933797909, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24650267, Length=573, Percent_Identity=31.064572425829, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI18859993, Length=578, Percent_Identity=33.5640138408304, Blast_Score=244, Evalue=1e-64,
Organism=Drosophila melanogaster, GI24642051, Length=561, Percent_Identity=31.5508021390374, Blast_Score=241, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24645930, Length=573, Percent_Identity=27.9232111692845, Blast_Score=150, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24642057, Length=592, Percent_Identity=27.1959459459459, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 56867; Mature: 56867

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS00623 GMC_OXRED_1 ; PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQFDIIVIGGGSAGSAAAGRLAEDGARTVCLVEAGGTNDIVRVKTPGFMPFIPKSSNWR
CCCEEEEEEECCCCCCHHHCCHHCCCCCEEEEEECCCCCCEEEEECCCCCEECCCCCCCC
YDTVPQQGLNGRIGYQPRGRGLGGSSAINAMVYIRGHAFDYDQWAALGATGWSYADVLPY
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHCCCCCCHHHHHHH
FKRSEGNERGGDEFHGGDGPLNVMDQRWPNVTSRRFVESATALQLPRTADFNGPDNEGFG
HHCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
LYQVTQKGGERWSAARAYVEPLRGRSNFDIRTGALVEKILIEEGRAVGVTIRCGRRRETL
EEEEEHHCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCEEEEEEECCCCHHHH
RARGGVVLSAGAFGSPQILMLSGIGPGAHLQEMGIAVARDHAGVGDNLQDHIDYVSSWET
HHCCCEEEECCCCCCCCEEEEECCCCCCHHHHHCCEEEECCCCCCCCHHHHHHHHHCCCC
RSTDPFGDSFGGTWRMVKAIVEHRRRRTGIMTTCFAEAGGFWKSRPDLPAPDVQYHFVPA
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCCCCCCCCEEEEEHH
MLEDHGRTKVKGHGFSCHACVLRPESRGTVRLASSDAAAAPTIDPGFLTDERDMATLRAG
HHHCCCCCEEECCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
VRMMHRIVAAPPLADYAGVDRHPVNLDDDAALDALIRSRADTVYHPVGTCRMGSDADAVV
HHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCCEE
DPTLKLNGIDGLWVADASIMPRLVSGNTNAPSIMIGERAADFVKAALS
CCCEEECCCCCEEEECHHHCCCEECCCCCCCEEEECCHHHHHHHHHCC
>Mature Secondary Structure
MDQFDIIVIGGGSAGSAAAGRLAEDGARTVCLVEAGGTNDIVRVKTPGFMPFIPKSSNWR
CCCEEEEEEECCCCCCHHHCCHHCCCCCEEEEEECCCCCCEEEEECCCCCEECCCCCCCC
YDTVPQQGLNGRIGYQPRGRGLGGSSAINAMVYIRGHAFDYDQWAALGATGWSYADVLPY
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHHHHCCCCCCHHHHHHH
FKRSEGNERGGDEFHGGDGPLNVMDQRWPNVTSRRFVESATALQLPRTADFNGPDNEGFG
HHCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCE
LYQVTQKGGERWSAARAYVEPLRGRSNFDIRTGALVEKILIEEGRAVGVTIRCGRRRETL
EEEEEHHCCHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCEEEEEEECCCCHHHH
RARGGVVLSAGAFGSPQILMLSGIGPGAHLQEMGIAVARDHAGVGDNLQDHIDYVSSWET
HHCCCEEEECCCCCCCCEEEEECCCCCCHHHHHCCEEEECCCCCCCCHHHHHHHHHCCCC
RSTDPFGDSFGGTWRMVKAIVEHRRRRTGIMTTCFAEAGGFWKSRPDLPAPDVQYHFVPA
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHCCCCCCCCCCCCCCCEEEEEHH
MLEDHGRTKVKGHGFSCHACVLRPESRGTVRLASSDAAAAPTIDPGFLTDERDMATLRAG
HHHCCCCCEEECCCCEEEEEEECCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHH
VRMMHRIVAAPPLADYAGVDRHPVNLDDDAALDALIRSRADTVYHPVGTCRMGSDADAVV
HHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCCCCEE
DPTLKLNGIDGLWVADASIMPRLVSGNTNAPSIMIGERAADFVKAALS
CCCEEECCCCCEEEECHHHCCCEECCCCCCCEEEECCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]