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The map label for this gene is rluE [H]

Identifier: 103487783

GI number: 103487783

Start: 2429035

End: 2429592

Strand: Reverse

Name: rluE [H]

Synonym: Sala_2302

Alternate gene names: 103487783

Gene position: 2429592-2429035 (Counterclockwise)

Preceding gene: 103487784

Following gene: 103487779

Centisome position: 72.63

GC content: 70.79

Gene sequence:

>558_bases
TTGCCCCGCCTGATCCTGTTCAACAAGCCGTACGGCGTGCTGTCGCAATTCACCGATCGCGGCGGCGCGGACGGGACCGG
CGGCGGGCGCGCGACGCTGTCCGACTATATCGACGTGCCGGGCGTCTATCCCGCCGGGCGGCTGGACAAGGACAGCGAAG
GCCTGCTCGTCCTGACCGACGACGGCGCGCTCCAGGCGCGGATATCGTCGCCGAAGCACAAGATGCCCAAAACCTATCTG
GCGCAGGTCGAGGGCGAGCCCGATGACGCCGCGCTGGATGCGCTCGCCCGCGGCGTCACGCTGAACGACGGCCCGACCCG
CCCCGCGACCGTCCGCCGCATCGGGGCGCCAAAGCTGTGGGACCGCGACCCGCCGGTGCGATACCGCAAGAGCGTGCCCG
ACTCATGGCTCGAACTCACCATCACCGAAGGCCGCAACCGCCAGGTGCGGCGGATGACGGCGGCGGTCGGCCATCCGACG
CTGCGGCTGGTGCGCTGGCGGATCGGGGGGTGGGAGATCGGGGATTTGGCGGTGGGGGCGTGGCGGGAGGTGAAATAG

Upstream 100 bases:

>100_bases
GAAGTCGAGACACGCGAGGGCAGGCGCGTTCCAGGCGTGTCTCGACTTCGCTCGACACGAATGGTTACAGTTGGGTTTCT
TGATAGCCGAGGAGGCCCGG

Downstream 100 bases:

>100_bases
CCGCTGCCTAGCAGCTGTCGGCGGCCAGAAAATTTTTTGGGCGTAGATTAACAAGAAGGCGACGTTCGCGGCTATGGCGA
CTGGTCGGCGGTCAGGGCGC

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MPRLILFNKPYGVLSQFTDRGGADGTGGGRATLSDYIDVPGVYPAGRLDKDSEGLLVLTDDGALQARISSPKHKMPKTYL
AQVEGEPDDAALDALARGVTLNDGPTRPATVRRIGAPKLWDRDPPVRYRKSVPDSWLELTITEGRNRQVRRMTAAVGHPT
LRLVRWRIGGWEIGDLAVGAWREVK

Sequences:

>Translated_185_residues
MPRLILFNKPYGVLSQFTDRGGADGTGGGRATLSDYIDVPGVYPAGRLDKDSEGLLVLTDDGALQARISSPKHKMPKTYL
AQVEGEPDDAALDALARGVTLNDGPTRPATVRRIGAPKLWDRDPPVRYRKSVPDSWLELTITEGRNRQVRRMTAAVGHPT
LRLVRWRIGGWEIGDLAVGAWREVK
>Mature_184_residues
PRLILFNKPYGVLSQFTDRGGADGTGGGRATLSDYIDVPGVYPAGRLDKDSEGLLVLTDDGALQARISSPKHKMPKTYLA
QVEGEPDDAALDALARGVTLNDGPTRPATVRRIGAPKLWDRDPPVRYRKSVPDSWLELTITEGRNRQVRRMTAAVGHPTL
RLVRWRIGGWEIGDLAVGAWREVK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=183, Percent_Identity=59.5628415300546, Blast_Score=211, Evalue=2e-56,
Organism=Escherichia coli, GI1788510, Length=187, Percent_Identity=36.3636363636364, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1787524, Length=174, Percent_Identity=35.0574712643678, Blast_Score=86, Evalue=1e-18,
Organism=Escherichia coli, GI1790453, Length=182, Percent_Identity=30.2197802197802, Blast_Score=74, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 20324; Mature: 20193

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRLILFNKPYGVLSQFTDRGGADGTGGGRATLSDYIDVPGVYPAGRLDKDSEGLLVLTD
CCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEC
DGALQARISSPKHKMPKTYLAQVEGEPDDAALDALARGVTLNDGPTRPATVRRIGAPKLW
CCCEEEEECCCCCCCCHHEEEEECCCCCHHHHHHHHCCCEECCCCCCCHHEEECCCCCCC
DRDPPVRYRKSVPDSWLELTITEGRNRQVRRMTAAVGHPTLRLVRWRIGGWEIGDLAVGA
CCCCCCHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCEEECCEEECC
WREVK
EECCC
>Mature Secondary Structure 
PRLILFNKPYGVLSQFTDRGGADGTGGGRATLSDYIDVPGVYPAGRLDKDSEGLLVLTD
CCEEEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCEEEEEC
DGALQARISSPKHKMPKTYLAQVEGEPDDAALDALARGVTLNDGPTRPATVRRIGAPKLW
CCCEEEEECCCCCCCCHHEEEEECCCCCHHHHHHHHCCCEECCCCCCCHHEEECCCCCCC
DRDPPVRYRKSVPDSWLELTITEGRNRQVRRMTAAVGHPTLRLVRWRIGGWEIGDLAVGA
CCCCCCHHHHCCCCCEEEEEEECCCCCHHHHHHHHHCCCEEEEEEEECCCEEECCEEECC
WREVK
EECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]