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The map label for this gene is ysgA [H]

Identifier: 103487766

GI number: 103487766

Start: 2408501

End: 2409193

Strand: Reverse

Name: ysgA [H]

Synonym: Sala_2285

Alternate gene names: 103487766

Gene position: 2409193-2408501 (Counterclockwise)

Preceding gene: 103487774

Following gene: 103487764

Centisome position: 72.02

GC content: 64.5

Gene sequence:

>693_bases
ATGAGAGGGAAATCCATGGGTGAGATGATCCGGATGACGATGGACGATGGCGCCGCCGTCGCTGTCTATCACGCGCAGCC
CGTGGGAGAGCGCCGCGGCGGGCTGGTGCTGGTGCAGGAAATCTTCGGCGTCACCGACCATATCCGCGAGCTTTGCGACG
AATATGCCGCCGACGGTTACGAGGCGCTCGCCCCCGCGCTCTTCGACCGCGAACATCCGGGGTTCGAAAGCGATTATTCG
GGCCCGCAGTTCGAGCGCGCGGTGCAGCTGGCGCGCGAGCTGCACCCATTCGAGCAGAGTTTGAAGGATGCCCAGACGTG
CATCGACGCATTGAAAGGCAAAGGGCCGGTGTTCATCACCGGCTATTGCTATGGCGGGTCGGTCGCGTGGCGGATGGCGC
AGATCAGCCCCGACCTTGCGGCGGCATCCGCCTATTATGGCAGCCTCGTGCCGACGATGTTCAGCGACGAGCCGCCGCGC
TGCGCGACCATCGCCCATTTCGGCCGTTTCGACGGCGGCATCCCGATGGAAGGCGTCGAGGCGCTGATCGCGAAGAACCA
CCCGACCGCGCAAATCTTTGTCTATGAGGCGGGGCACGGGTTCAACAGCGACCGGCGCAAGGATTATCACGAACCGAGCG
CGGATCTGGCGCGCGAACGCACGCTGATGCTGTTCAGGGCATGCGGCGGGTAG

Upstream 100 bases:

>100_bases
GGCAGGCTCCAGGCTTGTCGAAGGGCCGCCCTTCCTTCAAGGATTGAAAGAAAGAAGACCGCTGCTTCGACAACCCCGGA
TCGAGTCCGGGGACGAACCG

Downstream 100 bases:

>100_bases
TGGCGGCTGCGTCTTTCAAGGCGTCGCGGGCGACAGGACGTCATTCACCCGCCAACGCACCCTCCACCGCCGCACCCGAT
GCGCGCTGGCAGCGATAGAC

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MRGKSMGEMIRMTMDDGAAVAVYHAQPVGERRGGLVLVQEIFGVTDHIRELCDEYAADGYEALAPALFDREHPGFESDYS
GPQFERAVQLARELHPFEQSLKDAQTCIDALKGKGPVFITGYCYGGSVAWRMAQISPDLAAASAYYGSLVPTMFSDEPPR
CATIAHFGRFDGGIPMEGVEALIAKNHPTAQIFVYEAGHGFNSDRRKDYHEPSADLARERTLMLFRACGG

Sequences:

>Translated_230_residues
MRGKSMGEMIRMTMDDGAAVAVYHAQPVGERRGGLVLVQEIFGVTDHIRELCDEYAADGYEALAPALFDREHPGFESDYS
GPQFERAVQLARELHPFEQSLKDAQTCIDALKGKGPVFITGYCYGGSVAWRMAQISPDLAAASAYYGSLVPTMFSDEPPR
CATIAHFGRFDGGIPMEGVEALIAKNHPTAQIFVYEAGHGFNSDRRKDYHEPSADLARERTLMLFRACGG
>Mature_230_residues
MRGKSMGEMIRMTMDDGAAVAVYHAQPVGERRGGLVLVQEIFGVTDHIRELCDEYAADGYEALAPALFDREHPGFESDYS
GPQFERAVQLARELHPFEQSLKDAQTCIDALKGKGPVFITGYCYGGSVAWRMAQISPDLAAASAYYGSLVPTMFSDEPPR
CATIAHFGRFDGGIPMEGVEALIAKNHPTAQIFVYEAGHGFNSDRRKDYHEPSADLARERTLMLFRACGG

Specific function: Unknown

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=241, Percent_Identity=35.6846473029046, Blast_Score=109, Evalue=1e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 25246; Mature: 25246

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGKSMGEMIRMTMDDGAAVAVYHAQPVGERRGGLVLVQEIFGVTDHIRELCDEYAADGY
CCCCHHHHHHHHEECCCCEEEEEECCCCCCCCCCEEEHHHHHCHHHHHHHHHHHHHCCCH
EALAPALFDREHPGFESDYSGPQFERAVQLARELHPFEQSLKDAQTCIDALKGKGPVFIT
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEE
GYCYGGSVAWRMAQISPDLAAASAYYGSLVPTMFSDEPPRCATIAHFGRFDGGIPMEGVE
EEEECCHHHHHHHHCCCCHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHCCCCCCCHHHHH
ALIAKNHPTAQIFVYEAGHGFNSDRRKDYHEPSADLARERTLMLFRACGG
HHHHCCCCCEEEEEEECCCCCCCCHHHCCCCCCHHHHHHHHHHHHEECCC
>Mature Secondary Structure
MRGKSMGEMIRMTMDDGAAVAVYHAQPVGERRGGLVLVQEIFGVTDHIRELCDEYAADGY
CCCCHHHHHHHHEECCCCEEEEEECCCCCCCCCCEEEHHHHHCHHHHHHHHHHHHHCCCH
EALAPALFDREHPGFESDYSGPQFERAVQLARELHPFEQSLKDAQTCIDALKGKGPVFIT
HHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEE
GYCYGGSVAWRMAQISPDLAAASAYYGSLVPTMFSDEPPRCATIAHFGRFDGGIPMEGVE
EEEECCHHHHHHHHCCCCHHHHHHHHHHHCHHHHCCCCCCHHHHHHHHCCCCCCCHHHHH
ALIAKNHPTAQIFVYEAGHGFNSDRRKDYHEPSADLARERTLMLFRACGG
HHHHCCCCCEEEEEEECCCCCCCCHHHCCCCCCHHHHHHHHHHHHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]