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The map label for this gene is xthA [H]

Identifier: 103487661

GI number: 103487661

Start: 2290718

End: 2291488

Strand: Reverse

Name: xthA [H]

Synonym: Sala_2180

Alternate gene names: 103487661

Gene position: 2291488-2290718 (Counterclockwise)

Preceding gene: 103487662

Following gene: 103487659

Centisome position: 68.5

GC content: 65.63

Gene sequence:

>771_bases
ATGAAAATCGCGACCTTCAATATCAACGGGATCAAGGCCCGCCTGCCGCGCCTGATCGAATGGCTGGAGGAAAGGCAGCC
CGATGTCGCCTGCCTTCAGGAACTGAAATCGAGCGACGAGACGATGCCGACGAAGGAGATCGAGGCAGCGGGTTACGGCT
TCCTCTATCACGGACAAAAAGGCTTCAACGGCGTCGCGATCCTGGCGAAAGGCGCCGATCCGGTCGAGGTGCAGCGCGGC
CTTGCAGGCGAGGCCGAGGACGAGCAGTCGCGCTATCTGGAAGCCGACGCCCACGGCATCCGCGTCGCGTCCATCTATCT
GCCCAACGGCAATCCGCAGCCGGGGCCGAAGTTCGATTACAAGCTGCGCTGGATGGCGCGGCTGCGCGAACGCGCCAGGA
TGCTCCTCGCCGCCGAAATCCCCACCGTGCTCGCGGGCGATTACAACGTCATCCCGCACGACGACGATGTCTGGGATCCC
CGCGCCATGGCCACCGACGCGCTGATGCAGCCCGAATCGCGCGATGCCTGGTTCCGCCTGCTCGGCGACGGCTGGACCGA
CGCGCTGCGCAGCCGCCATCCGGCGGGCCATGTCTGGACCTATTGGGACTATCAGGCGGGCGCCTGGCAGCGCGACCACG
GGTTCCGCATCGACCATCTGCTTTTGAGTCCGGCGCTCGCCGACCGGCTGGTCGATGCCGGCGTCGACAAGGATCATCGC
GGACGCGAAAAGGCGAGCGATCATGCGCCCACCTGGGTGGTGCTGTCCTGA

Upstream 100 bases:

>100_bases
GCAATGACGAAATTGCGGACGTCAAAGGAATGATCGCCAAACAAGTTCCGGGTGACGAAGGGGCTGTGATCGGCGTCGGC
TTGCTGGCATAGGTGCGGGC

Downstream 100 bases:

>100_bases
CGCCCCGGATCAGAGCGGCTTTTCGTAAATCTGATACACGCGGTTGATGCGGGCATCGATCGCGGCCGCGACCGCGTTCA
TTCCCTGATTGTCGTCGAGC

Product: exodeoxyribonuclease III (xth)

Products: NA

Alternate protein names: EXO III; Exonuclease III [H]

Number of amino acids: Translated: 256; Mature: 256

Protein sequence:

>256_residues
MKIATFNINGIKARLPRLIEWLEERQPDVACLQELKSSDETMPTKEIEAAGYGFLYHGQKGFNGVAILAKGADPVEVQRG
LAGEAEDEQSRYLEADAHGIRVASIYLPNGNPQPGPKFDYKLRWMARLRERARMLLAAEIPTVLAGDYNVIPHDDDVWDP
RAMATDALMQPESRDAWFRLLGDGWTDALRSRHPAGHVWTYWDYQAGAWQRDHGFRIDHLLLSPALADRLVDAGVDKDHR
GREKASDHAPTWVVLS

Sequences:

>Translated_256_residues
MKIATFNINGIKARLPRLIEWLEERQPDVACLQELKSSDETMPTKEIEAAGYGFLYHGQKGFNGVAILAKGADPVEVQRG
LAGEAEDEQSRYLEADAHGIRVASIYLPNGNPQPGPKFDYKLRWMARLRERARMLLAAEIPTVLAGDYNVIPHDDDVWDP
RAMATDALMQPESRDAWFRLLGDGWTDALRSRHPAGHVWTYWDYQAGAWQRDHGFRIDHLLLSPALADRLVDAGVDKDHR
GREKASDHAPTWVVLS
>Mature_256_residues
MKIATFNINGIKARLPRLIEWLEERQPDVACLQELKSSDETMPTKEIEAAGYGFLYHGQKGFNGVAILAKGADPVEVQRG
LAGEAEDEQSRYLEADAHGIRVASIYLPNGNPQPGPKFDYKLRWMARLRERARMLLAAEIPTVLAGDYNVIPHDDDVWDP
RAMATDALMQPESRDAWFRLLGDGWTDALRSRHPAGHVWTYWDYQAGAWQRDHGFRIDHLLLSPALADRLVDAGVDKDHR
GREKASDHAPTWVVLS

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction [H]

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=263, Percent_Identity=28.5171102661597, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI18375503, Length=263, Percent_Identity=28.5171102661597, Blast_Score=120, Evalue=9e-28,
Organism=Homo sapiens, GI18375501, Length=263, Percent_Identity=28.5171102661597, Blast_Score=120, Evalue=9e-28,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=35.5805243445693, Blast_Score=150, Evalue=8e-38,
Organism=Caenorhabditis elegans, GI71989536, Length=257, Percent_Identity=26.0700389105058, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI221330655, Length=264, Percent_Identity=29.1666666666667, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17136678, Length=264, Percent_Identity=29.1666666666667, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 28876; Mature: 28876

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIATFNINGIKARLPRLIEWLEERQPDVACLQELKSSDETMPTKEIEAAGYGFLYHGQK
CEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHCCCEEEEECCC
GFNGVAILAKGADPVEVQRGLAGEAEDEQSRYLEADAHGIRVASIYLPNGNPQPGPKFDY
CCCCEEEEECCCCHHHHHHCCCCCCCCHHHHEEECCCCCEEEEEEEECCCCCCCCCCCCH
KLRWMARLRERARMLLAAEIPTVLAGDYNVIPHDDDVWDPRAMATDALMQPESRDAWFRL
HHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHH
LGDGWTDALRSRHPAGHVWTYWDYQAGAWQRDHGFRIDHLLLSPALADRLVDAGVDKDHR
HCCCHHHHHHCCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
GREKASDHAPTWVVLS
CCHHCCCCCCEEEEEC
>Mature Secondary Structure
MKIATFNINGIKARLPRLIEWLEERQPDVACLQELKSSDETMPTKEIEAAGYGFLYHGQK
CEEEEEECCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHCCCEEEEECCC
GFNGVAILAKGADPVEVQRGLAGEAEDEQSRYLEADAHGIRVASIYLPNGNPQPGPKFDY
CCCCEEEEECCCCHHHHHHCCCCCCCCHHHHEEECCCCCEEEEEEEECCCCCCCCCCCCH
KLRWMARLRERARMLLAAEIPTVLAGDYNVIPHDDDVWDPRAMATDALMQPESRDAWFRL
HHHHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCHHHHH
LGDGWTDALRSRHPAGHVWTYWDYQAGAWQRDHGFRIDHLLLSPALADRLVDAGVDKDHR
HCCCHHHHHHCCCCCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
GREKASDHAPTWVVLS
CCHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]