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The map label for this gene is yogA [H]

Identifier: 103487201

GI number: 103487201

Start: 1806504

End: 1807481

Strand: Reverse

Name: yogA [H]

Synonym: Sala_1716

Alternate gene names: 103487201

Gene position: 1807481-1806504 (Counterclockwise)

Preceding gene: 103487204

Following gene: 103487200

Centisome position: 54.03

GC content: 71.37

Gene sequence:

>978_bases
ATGCGAGCCTTGCAGGTGCGCGAACTATTGCCCGATCATGCGGGCACGGGGCTGGCCGATCTCCCGGTTCCGCTCGCCGG
GCCGGGGGAGGTACGCGTGCGGGTGCGCGCGGCGGGGGTGAACTTTCCCGACCTGTTGATGACGCGCGGCGCTTATCAGC
TCAAACCGGCGCTGCCGTTCGTTCCGGGCCTGGAGTTTGCGGGCGAGGTCGATGCGGTGGGCGAGGGGGTGAAAAGCTGG
TCGGTGGGCGATGCGGTCGTGGGCGGCAACCGCTTCGGGGCGATGGCTGAATATACGGTGGTGCCCGCGGCGTCGCTGCG
CCCCAGGCCCGCGGCGCTCGGCTGGGACGCGGCGGCCGCCTATCCGGTTGCTTATCTCACCGCCTATGTCGCGCTGGTCC
GCGGGGCGCGGATCGAACCGGGCGATTGGGTGCTGGTCCACGGTGCGGCGGGCGGGGTGGGGCTCGCGACCGTCGATCTG
GCGAAGGCGCTCGGCGCGCGGGTGATCGCGGCGGCGAGCCGCGCGGACAAGCGCGCGGCGGTCGAGCGGCTTTACGCGCC
CGATGCCGTGATCGACGGCGCGCCGGGCTTTCGCGAGACGGTGAAGGCGCTGACCGGCGGGCAGGGCGCCGATGTCATCG
TTGATCCGGTCGGCGGCGATATATTCGACGAATCGACGCGCTGCATCGCCTTTGGCGGGCGCTTGCTCGTCGTCGGCTTT
GCGTCGGGGCGCATCCCCGAACTGCCCGCCAACATGCCGCTGATCAAGGGATTTTCGGTCGTCGGCGTGCGCGCGGGCGA
ATATGGCCGCCGCTTTCCCGAGCGCGGCGCGGAGAATGTCGCGGCGATCGACGCGCTTGCGGCGGCGGGGACGATCCGCC
CGCATGTCCATGCGACGCTCGACCTGGCCGACTGGCGCGAGGGGTTTGCGATGCTGGAACGACGCGAGGCGGTCGGCACG
GTCGTGCTGCGGCCGTGA

Upstream 100 bases:

>100_bases
CGCAGGTCGGGGATTGGGATCTGGGCATGATTCGCTCCTGTTGGTTCGGTTGAGCGGAGCAGCGAACCATATAGGAACAA
TGTAGGAAAGGATTTTCGCC

Downstream 100 bases:

>100_bases
CGGGATGCGCTGCCGCCAAAGGACGGCGGCGAATGGCCGCGATGCCGAAGAAATTGGTCGGGACGAGAGGATTCGAACCT
CCGACCCCCACACCCCCAGT

Product: zinc-binding alcohol dehydrogenase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MRALQVRELLPDHAGTGLADLPVPLAGPGEVRVRVRAAGVNFPDLLMTRGAYQLKPALPFVPGLEFAGEVDAVGEGVKSW
SVGDAVVGGNRFGAMAEYTVVPAASLRPRPAALGWDAAAAYPVAYLTAYVALVRGARIEPGDWVLVHGAAGGVGLATVDL
AKALGARVIAAASRADKRAAVERLYAPDAVIDGAPGFRETVKALTGGQGADVIVDPVGGDIFDESTRCIAFGGRLLVVGF
ASGRIPELPANMPLIKGFSVVGVRAGEYGRRFPERGAENVAAIDALAAAGTIRPHVHATLDLADWREGFAMLERREAVGT
VVLRP

Sequences:

>Translated_325_residues
MRALQVRELLPDHAGTGLADLPVPLAGPGEVRVRVRAAGVNFPDLLMTRGAYQLKPALPFVPGLEFAGEVDAVGEGVKSW
SVGDAVVGGNRFGAMAEYTVVPAASLRPRPAALGWDAAAAYPVAYLTAYVALVRGARIEPGDWVLVHGAAGGVGLATVDL
AKALGARVIAAASRADKRAAVERLYAPDAVIDGAPGFRETVKALTGGQGADVIVDPVGGDIFDESTRCIAFGGRLLVVGF
ASGRIPELPANMPLIKGFSVVGVRAGEYGRRFPERGAENVAAIDALAAAGTIRPHVHATLDLADWREGFAMLERREAVGT
VVLRP
>Mature_325_residues
MRALQVRELLPDHAGTGLADLPVPLAGPGEVRVRVRAAGVNFPDLLMTRGAYQLKPALPFVPGLEFAGEVDAVGEGVKSW
SVGDAVVGGNRFGAMAEYTVVPAASLRPRPAALGWDAAAAYPVAYLTAYVALVRGARIEPGDWVLVHGAAGGVGLATVDL
AKALGARVIAAASRADKRAAVERLYAPDAVIDGAPGFRETVKALTGGQGADVIVDPVGGDIFDESTRCIAFGGRLLVVGF
ASGRIPELPANMPLIKGFSVVGVRAGEYGRRFPERGAENVAAIDALAAAGTIRPHVHATLDLADWREGFAMLERREAVGT
VVLRP

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI18379349, Length=311, Percent_Identity=29.903536977492, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI194239674, Length=264, Percent_Identity=31.0606060606061, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI13236495, Length=264, Percent_Identity=31.0606060606061, Blast_Score=126, Evalue=2e-29,
Organism=Homo sapiens, GI22538446, Length=311, Percent_Identity=27.6527331189711, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI22538444, Length=311, Percent_Identity=27.6527331189711, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI24308257, Length=322, Percent_Identity=27.0186335403727, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI194239676, Length=182, Percent_Identity=34.6153846153846, Blast_Score=106, Evalue=3e-23,
Organism=Homo sapiens, GI28557745, Length=285, Percent_Identity=35.0877192982456, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI41872631, Length=314, Percent_Identity=28.0254777070064, Blast_Score=95, Evalue=8e-20,
Organism=Homo sapiens, GI67078404, Length=316, Percent_Identity=25.9493670886076, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI47519420, Length=332, Percent_Identity=28.3132530120482, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI67078406, Length=279, Percent_Identity=25.8064516129032, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1790485, Length=301, Percent_Identity=31.8936877076412, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1787863, Length=353, Percent_Identity=25.7790368271955, Blast_Score=62, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI17507255, Length=179, Percent_Identity=40.7821229050279, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71988145, Length=181, Percent_Identity=27.0718232044199, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71987554, Length=184, Percent_Identity=31.5217391304348, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17536829, Length=272, Percent_Identity=26.8382352941176, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6319520, Length=333, Percent_Identity=29.4294294294294, Blast_Score=108, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319621, Length=340, Percent_Identity=28.2352941176471, Blast_Score=86, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6323961, Length=299, Percent_Identity=27.4247491638796, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324486, Length=292, Percent_Identity=27.7397260273973, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24581345, Length=237, Percent_Identity=27.4261603375527, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI221330659, Length=216, Percent_Identity=28.2407407407407, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI19920632, Length=216, Percent_Identity=28.2407407407407, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 33723; Mature: 33723

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00300 SRP54

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRALQVRELLPDHAGTGLADLPVPLAGPGEVRVRVRAAGVNFPDLLMTRGAYQLKPALPF
CCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCEEECCCCCC
VPGLEFAGEVDAVGEGVKSWSVGDAVVGGNRFGAMAEYTVVPAASLRPRPAALGWDAAAA
CCCCCCCCCHHHHHCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHH
YPVAYLTAYVALVRGARIEPGDWVLVHGAAGGVGLATVDLAKALGARVIAAASRADKRAA
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VERLYAPDAVIDGAPGFRETVKALTGGQGADVIVDPVGGDIFDESTRCIAFGGRLLVVGF
HHHHHCCCCEECCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCEEEEEEE
ASGRIPELPANMPLIKGFSVVGVRAGEYGRRFPERGAENVAAIDALAAAGTIRPHVHATL
CCCCCCCCCCCCCCCCCCEEEEEECCHHHHHCHHCCCCHHHHHHHHHHCCCCCCCEEEEE
DLADWREGFAMLERREAVGTVVLRP
EHHHHHHHHHHHHHHHHHCEEEECC
>Mature Secondary Structure
MRALQVRELLPDHAGTGLADLPVPLAGPGEVRVRVRAAGVNFPDLLMTRGAYQLKPALPF
CCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCHHHHHHCCCEEECCCCCC
VPGLEFAGEVDAVGEGVKSWSVGDAVVGGNRFGAMAEYTVVPAASLRPRPAALGWDAAAA
CCCCCCCCCHHHHHCCCCCCCCCCEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHH
YPVAYLTAYVALVRGARIEPGDWVLVHGAAGGVGLATVDLAKALGARVIAAASRADKRAA
HHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
VERLYAPDAVIDGAPGFRETVKALTGGQGADVIVDPVGGDIFDESTRCIAFGGRLLVVGF
HHHHHCCCCEECCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCEEEEECCEEEEEEE
ASGRIPELPANMPLIKGFSVVGVRAGEYGRRFPERGAENVAAIDALAAAGTIRPHVHATL
CCCCCCCCCCCCCCCCCCEEEEEECCHHHHHCHHCCCCHHHHHHHHHHCCCCCCCEEEEE
DLADWREGFAMLERREAVGTVVLRP
EHHHHHHHHHHHHHHHHHCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]