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The map label for this gene is atsB [H]

Identifier: 103487168

GI number: 103487168

Start: 1770770

End: 1772152

Strand: Reverse

Name: atsB [H]

Synonym: Sala_1683

Alternate gene names: 103487168

Gene position: 1772152-1770770 (Counterclockwise)

Preceding gene: 103487169

Following gene: 103487167

Centisome position: 52.98

GC content: 71.01

Gene sequence:

>1383_bases
GTGCCGCTGGAAGCCGCGCCGACGGACGGGCTGCCCGCCGATGTGCGGCAGGCCCTGCGCGCAAAGCCCGCCTTGCGTCC
GGCGGGATGCGACGGCGCGGCGGCGCTTGCGGCGCTGGGGGAGACGCAGCGGCGCCTGCGCACGCGGCTGGCGTCCGCCG
AGGCGCGGGCGTTCGATCAGCTGATCCACCTCGGCGCGGCACCCGCCAGCGGACTGGCGATGGTTCCCGCAGCGGCGCTC
GGCTTGACCGACCCGGCCTTTGCGGCGGCCGAACCAGCCGATCGCGCCGCGCCGCCGACCGGTCTCGGCAGTCTGGTGCT
GATCGCGAAGCTGACGCGCCATTGCAATTTGCGCTGCACCTATTGCACCGACTGGCGCGAAGGGCCGGGCAACCGGATGA
CCGCCGCGACGCAGGCGTTGATGGTGCGCCGTGTCGCCGAAGACCGGTCGGTCGCGACGGCGCGCTTCATTTGGCACGGC
GGCGAACCGAGTCTGCTCGGTGTGCGCAGGATGCTGGGCTTTCTGTGGTTGCAGGCGCGGCTGCGGCGACCGGGGCAGGA
TTTTACCAATTCGATCCAGACCAATGCCGCCGCGCTGCCCGACCCGGTGGTCGCGCTGTGGCGGCTGTTCGATTTCAAGG
TCAGTGTCAGCCTCGACGGCCCGCGCGCCATCCACGACCGCCAGCGGCGCACGGCACGCGGCAGCGCGTCGTTCGATGCG
GTGATCGACGGGATCGCCCGGCTGCGCGAGGCGGGGGTGTTCGCCGGCGTGCTGATCGTCGTGACACCGGAGGTTGCCGC
CTGCCCGGTCGAGAAGCTGGTCGGCGAGTTGGTCGACGCGGGCATCCATGCAGCAGGGTTCCTGCCAGTGCGGCCCGATG
CGGGTACGGGCGAGGGACCCTATCTGCCGCCCGCCGCCTTCGCCGATTTCCTGATCCGCCTGTTCGACCTGCTCCGCACG
CGCCCCGACCTGCGTTTCGATGTCCGCGAGATCGACGCGCTGCACGCCGCGTGGCAGCGGCAGGCGACGGGTTTCTGCGA
ACTCAATGGTCCCTGCCTTGGCGGTTATTTCGGGATCGAGCCCGACGGCACGGTGCGCCACTGCGACAAGTTTCTGGGGT
CGGCGGACCACGCGCTCGGCAATCTGGCCGAACGCAGCTTCGCCGACATCCGTGCAAGCGCGGAGATGACCGCGCTGAAG
CAGCGCGAGGCGGAACGCTGGCTGCGGCTGCGGACGTGCCGCTGGTACGGCCATTGCAAAGGCTGGTGCCCGCACGAAAG
CCACATTGCGGCGCGGCGCGGCGCGGCGGGGCGCTGTTGCGGGCTGGCGCCGGTCTTCGATCATTTCGAGACGCTTGCAG
GCGCCGAAAGGCAGCGGGCGTGA

Upstream 100 bases:

>100_bases
ACGGCTGGCGACCACGCTGCGGCCGGAGGAGGATGCCGTCGCGGTGCTGCTGCTAACGCGCGCGCGCGCGCTTCTGTCCT
TGCTGCGAACCGGCGCGTCG

Downstream 100 bases:

>100_bases
GCGCCGCCGTGGTCGCCTGCCACGCCTGCGCCGACGCGCGCCGCGCCTCGCTCGGCGCGGCCGCCGCGCCGCGTCGCGCG
TTGCTCGCGCTGCCGGGATG

Product: radical SAM family protein

Products: NA

Alternate protein names: AnSME; Arylsulfatase-activating protein; Ser-type sulfatase-activating enzyme [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MPLEAAPTDGLPADVRQALRAKPALRPAGCDGAAALAALGETQRRLRTRLASAEARAFDQLIHLGAAPASGLAMVPAAAL
GLTDPAFAAAEPADRAAPPTGLGSLVLIAKLTRHCNLRCTYCTDWREGPGNRMTAATQALMVRRVAEDRSVATARFIWHG
GEPSLLGVRRMLGFLWLQARLRRPGQDFTNSIQTNAAALPDPVVALWRLFDFKVSVSLDGPRAIHDRQRRTARGSASFDA
VIDGIARLREAGVFAGVLIVVTPEVAACPVEKLVGELVDAGIHAAGFLPVRPDAGTGEGPYLPPAAFADFLIRLFDLLRT
RPDLRFDVREIDALHAAWQRQATGFCELNGPCLGGYFGIEPDGTVRHCDKFLGSADHALGNLAERSFADIRASAEMTALK
QREAERWLRLRTCRWYGHCKGWCPHESHIAARRGAAGRCCGLAPVFDHFETLAGAERQRA

Sequences:

>Translated_460_residues
MPLEAAPTDGLPADVRQALRAKPALRPAGCDGAAALAALGETQRRLRTRLASAEARAFDQLIHLGAAPASGLAMVPAAAL
GLTDPAFAAAEPADRAAPPTGLGSLVLIAKLTRHCNLRCTYCTDWREGPGNRMTAATQALMVRRVAEDRSVATARFIWHG
GEPSLLGVRRMLGFLWLQARLRRPGQDFTNSIQTNAAALPDPVVALWRLFDFKVSVSLDGPRAIHDRQRRTARGSASFDA
VIDGIARLREAGVFAGVLIVVTPEVAACPVEKLVGELVDAGIHAAGFLPVRPDAGTGEGPYLPPAAFADFLIRLFDLLRT
RPDLRFDVREIDALHAAWQRQATGFCELNGPCLGGYFGIEPDGTVRHCDKFLGSADHALGNLAERSFADIRASAEMTALK
QREAERWLRLRTCRWYGHCKGWCPHESHIAARRGAAGRCCGLAPVFDHFETLAGAERQRA
>Mature_459_residues
PLEAAPTDGLPADVRQALRAKPALRPAGCDGAAALAALGETQRRLRTRLASAEARAFDQLIHLGAAPASGLAMVPAAALG
LTDPAFAAAEPADRAAPPTGLGSLVLIAKLTRHCNLRCTYCTDWREGPGNRMTAATQALMVRRVAEDRSVATARFIWHGG
EPSLLGVRRMLGFLWLQARLRRPGQDFTNSIQTNAAALPDPVVALWRLFDFKVSVSLDGPRAIHDRQRRTARGSASFDAV
IDGIARLREAGVFAGVLIVVTPEVAACPVEKLVGELVDAGIHAAGFLPVRPDAGTGEGPYLPPAAFADFLIRLFDLLRTR
PDLRFDVREIDALHAAWQRQATGFCELNGPCLGGYFGIEPDGTVRHCDKFLGSADHALGNLAERSFADIRASAEMTALKQ
REAERWLRLRTCRWYGHCKGWCPHESHIAARRGAAGRCCGLAPVFDHFETLAGAERQRA

Specific function: Involved in 'Ser-type' sulfatase maturation under anaerobic conditions. Catalyzes the post-translational modification of serine ('Ser-72' in the arylsulfatase AtsA) into 3-oxoalanine (also known as C(alpha)-formylglycine (FGly)), by a free radical chemica

COG id: COG0641

COG function: function code R; Arylsulfatase regulator (Fe-S oxidoreductase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Anaerobic sulfatase-maturating enzyme family [H]

Homologues:

Organism=Escherichia coli, GI48994973, Length=365, Percent_Identity=27.6712328767123, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI87081923, Length=355, Percent_Identity=23.943661971831, Blast_Score=99, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 49632; Mature: 49501

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS01305 MOAA_NIFB_PQQE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLEAAPTDGLPADVRQALRAKPALRPAGCDGAAALAALGETQRRLRTRLASAEARAFDQ
CCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIHLGAAPASGLAMVPAAALGLTDPAFAAAEPADRAAPPTGLGSLVLIAKLTRHCNLRCT
HHHHCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEE
YCTDWREGPGNRMTAATQALMVRRVAEDRSVATARFIWHGGEPSLLGVRRMLGFLWLQAR
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHH
LRRPGQDFTNSIQTNAAALPDPVVALWRLFDFKVSVSLDGPRAIHDRQRRTARGSASFDA
HCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCCHHHH
VIDGIARLREAGVFAGVLIVVTPEVAACPVEKLVGELVDAGIHAAGFLPVRPDAGTGEGP
HHHHHHHHHHHHHHHCEEEEECCCHHHCCHHHHHHHHHHCCCHHCCCEEECCCCCCCCCC
YLPPAAFADFLIRLFDLLRTRPDLRFDVREIDALHAAWQRQATGFCELNGPCLGGYFGIE
CCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCEEEECCCEECCCCCCC
PDGTVRHCDKFLGSADHALGNLAERSFADIRASAEMTALKQREAERWLRLRTCRWYGHCK
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GWCPHESHIAARRGAAGRCCGLAPVFDHFETLAGAERQRA
CCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHCCC
>Mature Secondary Structure 
PLEAAPTDGLPADVRQALRAKPALRPAGCDGAAALAALGETQRRLRTRLASAEARAFDQ
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIHLGAAPASGLAMVPAAALGLTDPAFAAAEPADRAAPPTGLGSLVLIAKLTRHCNLRCT
HHHHCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEE
YCTDWREGPGNRMTAATQALMVRRVAEDRSVATARFIWHGGEPSLLGVRRMLGFLWLQAR
EECCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHH
LRRPGQDFTNSIQTNAAALPDPVVALWRLFDFKVSVSLDGPRAIHDRQRRTARGSASFDA
HCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHEEEEEECCCCHHHHHHHHHHHCCCCHHHH
VIDGIARLREAGVFAGVLIVVTPEVAACPVEKLVGELVDAGIHAAGFLPVRPDAGTGEGP
HHHHHHHHHHHHHHHCEEEEECCCHHHCCHHHHHHHHHHCCCHHCCCEEECCCCCCCCCC
YLPPAAFADFLIRLFDLLRTRPDLRFDVREIDALHAAWQRQATGFCELNGPCLGGYFGIE
CCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHCCCEEEECCCEECCCCCCC
PDGTVRHCDKFLGSADHALGNLAERSFADIRASAEMTALKQREAERWLRLRTCRWYGHCK
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GWCPHESHIAARRGAAGRCCGLAPVFDHFETLAGAERQRA
CCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2180918 [H]