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The map label for this gene is degA [H]

Identifier: 103487147

GI number: 103487147

Start: 1747394

End: 1748407

Strand: Reverse

Name: degA [H]

Synonym: Sala_1662

Alternate gene names: 103487147

Gene position: 1748407-1747394 (Counterclockwise)

Preceding gene: 103487148

Following gene: 103487146

Centisome position: 52.27

GC content: 63.81

Gene sequence:

>1014_bases
ATGAGCGAGCGGGCCAATATCCGGGACGTCGCCGCCAAGGCGGGTGTGGCGGTCAAGACAGTGAGCCGCGTGCTCAACGG
TCATCCCTATGTCAGCGAAGAGACGCGGCGCCGCGTCGAGACGGCGATGGCGCAACTCGATTTCCGCCCAAGCGTTGCGG
CGCGCATCCTGTCGGGAGCGAAATCGAACCAGATTGCGCTGATCTATGACAATCACAGCCCCTATTACATGTTCCAGATC
CAGACGGGTTGCTGGCAACTTTGCAAGGAACGTGGCATCCGCCTGCTGGCGCAGCCGGTCGATGTGGCGGATCCCGGCGT
CGGGGACCAGGTGCGCGGGCTTGTCACCGAAACGCATGTCGACGGCATCATTCTGTCCTCTCCCGTGACCGATTGCGAAG
CAGTGCTCGCAGCGCTCGAGACGCTCGATATCCCGTTCGTTCGCATTTCACCGGGGACAAACCATTCGCTGACCAGCTCG
GTGTTCATGGACGACGTACAGGCGGCGGACGACATGACGACGCATCTGATCAACGTCGGCCATCGGCGGATCGGGTTCGT
CAAGGGCCATCCAAACCACCGCGCCTCCGATGACCGCCTGTACGGATATCGCCGGGCGCTCGATCGCGTCGGCATCGCGT
TTGAGCCCGGCCTCGTCTGTGACGGCGAGTTCGATTTCGACAGCGGGGTGGCGGCGGGACGCTTCCTGCTCGGCCTCGAC
AATCCACCGACCGCGATCTTTGCGGCGAATGACGATATGGCCGCCGGCGTCCTCGCGGTCGCGCATGACCGCGGCATCGA
GCTGCCTTCGGAGCTTTCGGTCGCGGGTTTCGACGACACGACCCTGTCGCGAACGGTGTGGCCACCGCTCACCACCATTC
ATCAGCCGATGGCCGAACTGGCGCGCACCGCGACGCGAATACTGATCGAGGGTGGCGACATCAGCCATCACCGCCTTCCC
CACACCCTTGTCGAGCGCGCTTCGGTCGCGCCACCTGCCAGGAATCCTCTATGA

Upstream 100 bases:

>100_bases
TTCACCAGATCCCCGCGCCCATTGGCATGCCACCGGTATTCTTCCGGCCCGACAGTGGCTTTGCGCCGCTGTTCGGCCGA
CTGGCGGAGATATGGAAACA

Downstream 100 bases:

>100_bases
GCTTGTTGCCCGTCCCGCCTGCATCGCGTCCGCTCGGAAACAGCGGCATGTCGGTTTCGTCCATCGCCTGGGGTATGTGG
CGGTTTGCCGAGGGTGGACG

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Degradation activator [H]

Number of amino acids: Translated: 337; Mature: 336

Protein sequence:

>337_residues
MSERANIRDVAAKAGVAVKTVSRVLNGHPYVSEETRRRVETAMAQLDFRPSVAARILSGAKSNQIALIYDNHSPYYMFQI
QTGCWQLCKERGIRLLAQPVDVADPGVGDQVRGLVTETHVDGIILSSPVTDCEAVLAALETLDIPFVRISPGTNHSLTSS
VFMDDVQAADDMTTHLINVGHRRIGFVKGHPNHRASDDRLYGYRRALDRVGIAFEPGLVCDGEFDFDSGVAAGRFLLGLD
NPPTAIFAANDDMAAGVLAVAHDRGIELPSELSVAGFDDTTLSRTVWPPLTTIHQPMAELARTATRILIEGGDISHHRLP
HTLVERASVAPPARNPL

Sequences:

>Translated_337_residues
MSERANIRDVAAKAGVAVKTVSRVLNGHPYVSEETRRRVETAMAQLDFRPSVAARILSGAKSNQIALIYDNHSPYYMFQI
QTGCWQLCKERGIRLLAQPVDVADPGVGDQVRGLVTETHVDGIILSSPVTDCEAVLAALETLDIPFVRISPGTNHSLTSS
VFMDDVQAADDMTTHLINVGHRRIGFVKGHPNHRASDDRLYGYRRALDRVGIAFEPGLVCDGEFDFDSGVAAGRFLLGLD
NPPTAIFAANDDMAAGVLAVAHDRGIELPSELSVAGFDDTTLSRTVWPPLTTIHQPMAELARTATRILIEGGDISHHRLP
HTLVERASVAPPARNPL
>Mature_336_residues
SERANIRDVAAKAGVAVKTVSRVLNGHPYVSEETRRRVETAMAQLDFRPSVAARILSGAKSNQIALIYDNHSPYYMFQIQ
TGCWQLCKERGIRLLAQPVDVADPGVGDQVRGLVTETHVDGIILSSPVTDCEAVLAALETLDIPFVRISPGTNHSLTSSV
FMDDVQAADDMTTHLINVGHRRIGFVKGHPNHRASDDRLYGYRRALDRVGIAFEPGLVCDGEFDFDSGVAAGRFLLGLDN
PPTAIFAANDDMAAGVLAVAHDRGIELPSELSVAGFDDTTLSRTVWPPLTTIHQPMAELARTATRILIEGGDISHHRLPH
TLVERASVAPPARNPL

Specific function: Involved in the control of degradation of B.subtilis amidophosphoribosyltransferase (purF). Probably activates the gene for a degradative protease [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=316, Percent_Identity=31.9620253164557, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1789068, Length=332, Percent_Identity=29.5180722891566, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=31.8181818181818, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI1788474, Length=346, Percent_Identity=33.2369942196532, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1787948, Length=336, Percent_Identity=30.3571428571429, Blast_Score=138, Evalue=7e-34,
Organism=Escherichia coli, GI1789202, Length=340, Percent_Identity=33.8235294117647, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1786540, Length=336, Percent_Identity=28.5714285714286, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1789456, Length=305, Percent_Identity=29.5081967213115, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787580, Length=311, Percent_Identity=27.6527331189711, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790715, Length=322, Percent_Identity=24.223602484472, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI48994940, Length=299, Percent_Identity=24.7491638795987, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787906, Length=316, Percent_Identity=26.8987341772152, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790689, Length=317, Percent_Identity=26.1829652996845, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36566; Mature: 36435

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSERANIRDVAAKAGVAVKTVSRVLNGHPYVSEETRRRVETAMAQLDFRPSVAARILSGA
CCCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC
KSNQIALIYDNHSPYYMFQIQTGCWQLCKERGIRLLAQPVDVADPGVGDQVRGLVTETHV
CCCEEEEEEECCCCEEEEEECHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHCC
DGIILSSPVTDCEAVLAALETLDIPFVRISPGTNHSLTSSVFMDDVQAADDMTTHLINVG
CCEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
HRRIGFVKGHPNHRASDDRLYGYRRALDRVGIAFEPGLVCDGEFDFDSGVAAGRFLLGLD
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEECCCEEECCCCCCCCCCCCCEEEEECC
NPPTAIFAANDDMAAGVLAVAHDRGIELPSELSVAGFDDTTLSRTVWPPLTTIHQPMAEL
CCCCEEEECCCCCHHHEEEEECCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHH
ARTATRILIEGGDISHHRLPHTLVERASVAPPARNPL
HHHHHEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SERANIRDVAAKAGVAVKTVSRVLNGHPYVSEETRRRVETAMAQLDFRPSVAARILSGA
CCCCCHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCC
KSNQIALIYDNHSPYYMFQIQTGCWQLCKERGIRLLAQPVDVADPGVGDQVRGLVTETHV
CCCEEEEEEECCCCEEEEEECHHHHHHHHHCCCEEEECCCCCCCCCCCHHHHHHHHHHCC
DGIILSSPVTDCEAVLAALETLDIPFVRISPGTNHSLTSSVFMDDVQAADDMTTHLINVG
CCEEEECCCHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
HRRIGFVKGHPNHRASDDRLYGYRRALDRVGIAFEPGLVCDGEFDFDSGVAAGRFLLGLD
CCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCEECCCEEECCCCCCCCCCCCCEEEEECC
NPPTAIFAANDDMAAGVLAVAHDRGIELPSELSVAGFDDTTLSRTVWPPLTTIHQPMAEL
CCCCEEEECCCCCHHHEEEEECCCCCCCCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHH
ARTATRILIEGGDISHHRLPHTLVERASVAPPARNPL
HHHHHEEEEECCCCCCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8407808; 9353932; 9384377 [H]