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The map label for this gene is wcaJ [H]

Identifier: 103487071

GI number: 103487071

Start: 1662430

End: 1662993

Strand: Reverse

Name: wcaJ [H]

Synonym: Sala_1586

Alternate gene names: 103487071

Gene position: 1662993-1662430 (Counterclockwise)

Preceding gene: 103487072

Following gene: 103487070

Centisome position: 49.71

GC content: 58.16

Gene sequence:

>564_bases
ATGATAAAAAGAGTATTCGACCTGATGCTCGCTTCGCTTACTGTCATTCTGCTGGCCCCGTTGATGCTTTGCGTGGCGCT
TGCGATCCGTCTGGATTCGGCGGGGCCAGCGGTGTTCTGGTCGCAGCGTTTTGGCCGTGACGGGCGATTGTTCCTGATGC
CGAAGTTTCGGACGATGCACATCGACACCCCGCTGCTTCCGACACATTTGCTTCGGGACGGCGCGAGCCGGGTGACGCGC
CTTGGGCGCTTTTTGCGGCGCACCAGCCTTGATGAGTTGCCGCAGCTTTTCAGTGTTATCGCCGGAGACATGAGCCTGGT
GGGGCCCCGGCCAGCGCTATTCAACCAAGATGATCTGATGCGTTTGAGGGCGGAATGCGGCGTTACCGCGCTTCGGCCTG
GAATAACCGGCTGGGCGCAGATCAACGGCCGCGACGAACTTACGATCTCAGAGAAAGTGACGTTGGAGCGTGAATATCTG
GCTCGAATGAGCCTCGCCTTCGACGTAAAGATCCTGATCCTTACGATAGTGCGGGTTCTGGATCGCCGCAGCATTTCGCA
TTGA

Upstream 100 bases:

>100_bases
GAAACGCGGCATTCGATGGTGTGCGTTTTACCCGCCTGTTCGGGTGGGCGCCGGAAATCCCGTTCGACAGGGCCATTCTG
ATGACGACGGGAACGGACGA

Downstream 100 bases:

>100_bases
TGTTTGCACTTGAAGATTTGGATCTCCAATCTTTTCATCCCGCGCTGAGGTCTGGACGTGTTTGAACGATTCTTCACTGA
TGCTTCGACCCAAATGATCA

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MIKRVFDLMLASLTVILLAPLMLCVALAIRLDSAGPAVFWSQRFGRDGRLFLMPKFRTMHIDTPLLPTHLLRDGASRVTR
LGRFLRRTSLDELPQLFSVIAGDMSLVGPRPALFNQDDLMRLRAECGVTALRPGITGWAQINGRDELTISEKVTLEREYL
ARMSLAFDVKILILTIVRVLDRRSISH

Sequences:

>Translated_187_residues
MIKRVFDLMLASLTVILLAPLMLCVALAIRLDSAGPAVFWSQRFGRDGRLFLMPKFRTMHIDTPLLPTHLLRDGASRVTR
LGRFLRRTSLDELPQLFSVIAGDMSLVGPRPALFNQDDLMRLRAECGVTALRPGITGWAQINGRDELTISEKVTLEREYL
ARMSLAFDVKILILTIVRVLDRRSISH
>Mature_187_residues
MIKRVFDLMLASLTVILLAPLMLCVALAIRLDSAGPAVFWSQRFGRDGRLFLMPKFRTMHIDTPLLPTHLLRDGASRVTR
LGRFLRRTSLDELPQLFSVIAGDMSLVGPRPALFNQDDLMRLRAECGVTALRPGITGWAQINGRDELTISEKVTLEREYL
ARMSLAFDVKILILTIVRVLDRRSISH

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=186, Percent_Identity=41.9354838709677, Blast_Score=144, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 21127; Mature: 21127

Theoretical pI: Translated: 11.24; Mature: 11.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKRVFDLMLASLTVILLAPLMLCVALAIRLDSAGPAVFWSQRFGRDGRLFLMPKFRTMH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCEEEE
IDTPLLPTHLLRDGASRVTRLGRFLRRTSLDELPQLFSVIAGDMSLVGPRPALFNQDDLM
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHH
RLRAECGVTALRPGITGWAQINGRDELTISEKVTLEREYLARMSLAFDVKILILTIVRVL
HHHHHCCCHHHCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRRSISH
HHHCCCC
>Mature Secondary Structure
MIKRVFDLMLASLTVILLAPLMLCVALAIRLDSAGPAVFWSQRFGRDGRLFLMPKFRTMH
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCEEEEECCEEEE
IDTPLLPTHLLRDGASRVTRLGRFLRRTSLDELPQLFSVIAGDMSLVGPRPALFNQDDLM
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHHCCCCCCCCCHHHHH
RLRAECGVTALRPGITGWAQINGRDELTISEKVTLEREYLARMSLAFDVKILILTIVRVL
HHHHHCCCHHHCCCCCCEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DRRSISH
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]