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The map label for this gene is bioD

Identifier: 103486959

GI number: 103486959

Start: 1533673

End: 1534281

Strand: Direct

Name: bioD

Synonym: Sala_1474

Alternate gene names: 103486959

Gene position: 1533673-1534281 (Clockwise)

Preceding gene: 103486958

Following gene: 103486960

Centisome position: 45.85

GC content: 66.01

Gene sequence:

>609_bases
ATGACGCGCTTCGTCGTCACCGGGACAGACACCGGCATCGGAAAAACCATTTTCTCCGCGAGCCTGGCGCGGGCGACGGG
CACACCCTACTGGAAACCCGTCCAGTCGGGGCTCGAAGAGGAAACCGACAGCGAAATTGTCGCGCGGCTCGCCGGGGTTC
CCGTCCGTCCCGAAGCCTATCGCCTCGTCACCCCGGCCTCACCGCACATCGCCGCCGAAATCGACGGCGTCGCCATTGAC
ATCGAAAGGCTGACGCCACCACCGGGCGAATTGATCGTCGAAGGTGCGGGTGGCGCGCTTGTCCCCGTCACCCGCCGGAC
GCTTTACGCCGAGCTTTTCGCGCGCTGGCAAGTGCCCGTGATCGTCTGCGCGCGTACCAGCCTCGGCACGATCAACCACA
GCCTGCTGACGATCGAGGCGCTGAAACGCCGGGACGTACCGATCCACGGCGTCGTATTCATCGGCGATGCGGTCGAGGAC
AGCGAGGCGATCATCGCGGACGTCTCGGGCGTTCGCCGGTTGGGCCGCTTGCCTGTCGTCGCCCCGCTGACGAGCGAAAA
TCTCGCCGCGGCGTTCGGCGCCAACTTCGACATCGCCGATTTCCGCTGA

Upstream 100 bases:

>100_bases
CCGAGGGAACGGCGCGCCTTCGCATCGCGATCACGCTCAATATCGACGAGAGCGACATCGACGATATGGCCGACGCGCTC
GCCGACGCGATGGCCGCGGC

Downstream 100 bases:

>100_bases
ATTGGCCATTTCCGCTTTCGCTGGAATGACGAAGGGATGTAAGGCGCGCCCGACATGGCCAAAAACCCTTCCCCCGTCTG
GCACCCCTTTACCCAGCACG

Product: dithiobiotin synthetase

Products: NA

Alternate protein names: DTB synthetase; DTBS; Dethiobiotin synthase

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MTRFVVTGTDTGIGKTIFSASLARATGTPYWKPVQSGLEEETDSEIVARLAGVPVRPEAYRLVTPASPHIAAEIDGVAID
IERLTPPPGELIVEGAGGALVPVTRRTLYAELFARWQVPVIVCARTSLGTINHSLLTIEALKRRDVPIHGVVFIGDAVED
SEAIIADVSGVRRLGRLPVVAPLTSENLAAAFGANFDIADFR

Sequences:

>Translated_202_residues
MTRFVVTGTDTGIGKTIFSASLARATGTPYWKPVQSGLEEETDSEIVARLAGVPVRPEAYRLVTPASPHIAAEIDGVAID
IERLTPPPGELIVEGAGGALVPVTRRTLYAELFARWQVPVIVCARTSLGTINHSLLTIEALKRRDVPIHGVVFIGDAVED
SEAIIADVSGVRRLGRLPVVAPLTSENLAAAFGANFDIADFR
>Mature_201_residues
TRFVVTGTDTGIGKTIFSASLARATGTPYWKPVQSGLEEETDSEIVARLAGVPVRPEAYRLVTPASPHIAAEIDGVAIDI
ERLTPPPGELIVEGAGGALVPVTRRTLYAELFARWQVPVIVCARTSLGTINHSLLTIEALKRRDVPIHGVVFIGDAVEDS
EAIIADVSGVRRLGRLPVVAPLTSENLAAAFGANFDIADFR

Specific function: Bioconversion of pimelate into dethiobiotin. [C]

COG id: COG0132

COG function: function code H; Dethiobiotin synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dethiobiotin synthetase family

Homologues:

Organism=Escherichia coli, GI1786995, Length=173, Percent_Identity=32.3699421965318, Blast_Score=74, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324385, Length=192, Percent_Identity=35.9375, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOD_SPHAL (Q1GT35)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616520.1
- ProteinModelPortal:   Q1GT35
- SMR:   Q1GT35
- GeneID:   4081246
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1474
- NMPDR:   fig|317655.9.peg.1407
- HOGENOM:   HBG650065
- OMA:   WKPIQSG
- ProtClustDB:   PRK00090
- BioCyc:   SALA317655:SALA_1474-MONOMER
- BRENDA:   6.3.3.3
- HAMAP:   MF_00336
- InterPro:   IPR004472
- PIRSF:   PIRSF006755
- TIGRFAMs:   TIGR00347

Pfam domain/function: NA

EC number: =6.3.3.3

Molecular weight: Translated: 21507; Mature: 21376

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRFVVTGTDTGIGKTIFSASLARATGTPYWKPVQSGLEEETDSEIVARLAGVPVRPEAY
CCEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCE
RLVTPASPHIAAEIDGVAIDIERLTPPPGELIVEGAGGALVPVTRRTLYAELFARWQVPV
EEECCCCCCEEEEECCEEEEEECCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHCCCCCE
IVCARTSLGTINHSLLTIEALKRRDVPIHGVVFIGDAVEDSEAIIADVSGVRRLGRLPVV
EEEECCCCCCCCCCEEEEEHHHHCCCCEEEEEEEECCCCCCCCEEHHHHHHHHHCCCCEE
APLTSENLAAAFGANFDIADFR
EECCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TRFVVTGTDTGIGKTIFSASLARATGTPYWKPVQSGLEEETDSEIVARLAGVPVRPEAY
CEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCHHHHHHHHHCCCCCCCCE
RLVTPASPHIAAEIDGVAIDIERLTPPPGELIVEGAGGALVPVTRRTLYAELFARWQVPV
EEECCCCCCEEEEECCEEEEEECCCCCCCCEEEECCCCEEEHHHHHHHHHHHHHCCCCCE
IVCARTSLGTINHSLLTIEALKRRDVPIHGVVFIGDAVEDSEAIIADVSGVRRLGRLPVV
EEEECCCCCCCCCCEEEEEHHHHCCCCEEEEEEEECCCCCCCCEEHHHHHHHHHCCCCEE
APLTSENLAAAFGANFDIADFR
EECCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA