Click here to switch to the map view.

The map label for this gene is dctD [H]

Identifier: 103486943

GI number: 103486943

Start: 1511965

End: 1513302

Strand: Direct

Name: dctD [H]

Synonym: Sala_1458

Alternate gene names: 103486943

Gene position: 1511965-1513302 (Clockwise)

Preceding gene: 103486942

Following gene: 103486951

Centisome position: 45.2

GC content: 67.26

Gene sequence:

>1338_bases
ATGAGTTTCTTCACCGCCGAGCAGACGATCCTTTTCGTCGACGACGATGCCGCACTGCGCGAAGCGAATGCGCAGACGCT
CGACCTTGCGGGCCTTGCGGTCGAAACCTTCGCCGATGCGCAGAGCGTGCTGCCGCGCATCGCCGCTGATTTCGCCGGGA
TCGTCATCACCGACATAAGGATGCCCGGCATGGACGGGCTGGAACTGCGCGCCGCGATCCATGCAATCGACCCCGACATC
CCCGTCGTGCTGATCACCGGGCACGCCGATGTGACGATGGCGGTGCGGGCGCTCCACGACGGGGCGTTCGACTTTCTCGC
CAAACCTTTTGCGGCGGACCATCTGGTCGGCGTCGCGCGCCGCGCGCTCGCGCATCGCGCGCTGATCCTCGACAACCGGC
GCCTCACCGCGGCGGCGGCGGCGATCGACAGTCCACTGATCGGCGAAAGCCCGGCGATGGTCCGTCTGCGCGAAACGATC
GCGCAGCTGGCGCAGGCCGACATCGACGTGCTGATCGAGGGCGAGACGGGGACGGGCAAGGAACTGGTCGCGCATATGCT
CCACCGCCAGAGTCGGCGGAGCGCGGCGTCGCTCGTCGCGGTCAATTGCGCCGCTTTGCCGCACGACCTGGCCGAAGCCG
AACTGTTCGGCAGCAACCTTGTCGACCGCGCGACGGGCAAGCTGGGCCAGGCTGGGCGGATCCGCGGCGCGCATCGCGGG
ACCTTGTTCCTCGACGAGATCGACACGATGCACGCGGCGGTGCAGGCGAAGCTGCTGCGCGTGATCGAGGAGCGCGAGGT
GCAGCCATTGGGGGCGAGTGCGCCCGAACCCGTCGACCTGCGCGTTGTCGCAGCGACCAAGACCGACCTGATGGACGCGG
TGCGTGCGGGGGATTTTCGCGAAGACCTTTATTACCGGCTGCACGTCGTCAAGCTGCGCCTTCCGCCCTTGCGCGAGCGT
CGCTCCGACATCCCGCAGACCTTTGCCTTCTTCCTCGACGAGGCGGCGTCGAAAATGGGGCTGACGGGGTTCCAGATCGA
CGACGCCACGCGTCGGCACCTGGTCGAACATGACTGGCCGGGCAACGTCCGCGAGCTCAAGAACTTCGCCTATAGCGTCG
TGCTCGACCTGCCCTCCGACCCCGCGCGTTCGGCGATGCCGGCAGCCGCGCCGCTTGCCGAGCGGCTGCGCCGGTTCGAG
GCGACGATCATCGAGGATACGCTCGCCCAGACGCGCGGCAATATCGGCGAGACGATGGCGCAGCTCGGCGTGCCGCGAAA
GACGCTTTACGACAAGATGGCCAAGCTGGGGATCGATCCGAAGCGGTTTCGAGGCTGA

Upstream 100 bases:

>100_bases
TCGCGATCGCGAGCGACATCGCCAAAGCGTTCGGCGGTACGCTCTCGATCATCCCCTCACCGCTCGGTGGTGCGGGGTTC
CGCGTGACGCTGAGGCCGGC

Downstream 100 bases:

>100_bases
TCGCAACGGAAGGAGCGACCTGTCAGTGTCGTCATCCCAGCGAAGGCCGGGATGACGATGATATGGGGCGGGCGTCAATC
CGGCTCGCGCTATTTCCCCT

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MSFFTAEQTILFVDDDAALREANAQTLDLAGLAVETFADAQSVLPRIAADFAGIVITDIRMPGMDGLELRAAIHAIDPDI
PVVLITGHADVTMAVRALHDGAFDFLAKPFAADHLVGVARRALAHRALILDNRRLTAAAAAIDSPLIGESPAMVRLRETI
AQLAQADIDVLIEGETGTGKELVAHMLHRQSRRSAASLVAVNCAALPHDLAEAELFGSNLVDRATGKLGQAGRIRGAHRG
TLFLDEIDTMHAAVQAKLLRVIEEREVQPLGASAPEPVDLRVVAATKTDLMDAVRAGDFREDLYYRLHVVKLRLPPLRER
RSDIPQTFAFFLDEAASKMGLTGFQIDDATRRHLVEHDWPGNVRELKNFAYSVVLDLPSDPARSAMPAAAPLAERLRRFE
ATIIEDTLAQTRGNIGETMAQLGVPRKTLYDKMAKLGIDPKRFRG

Sequences:

>Translated_445_residues
MSFFTAEQTILFVDDDAALREANAQTLDLAGLAVETFADAQSVLPRIAADFAGIVITDIRMPGMDGLELRAAIHAIDPDI
PVVLITGHADVTMAVRALHDGAFDFLAKPFAADHLVGVARRALAHRALILDNRRLTAAAAAIDSPLIGESPAMVRLRETI
AQLAQADIDVLIEGETGTGKELVAHMLHRQSRRSAASLVAVNCAALPHDLAEAELFGSNLVDRATGKLGQAGRIRGAHRG
TLFLDEIDTMHAAVQAKLLRVIEEREVQPLGASAPEPVDLRVVAATKTDLMDAVRAGDFREDLYYRLHVVKLRLPPLRER
RSDIPQTFAFFLDEAASKMGLTGFQIDDATRRHLVEHDWPGNVRELKNFAYSVVLDLPSDPARSAMPAAAPLAERLRRFE
ATIIEDTLAQTRGNIGETMAQLGVPRKTLYDKMAKLGIDPKRFRG
>Mature_444_residues
SFFTAEQTILFVDDDAALREANAQTLDLAGLAVETFADAQSVLPRIAADFAGIVITDIRMPGMDGLELRAAIHAIDPDIP
VVLITGHADVTMAVRALHDGAFDFLAKPFAADHLVGVARRALAHRALILDNRRLTAAAAAIDSPLIGESPAMVRLRETIA
QLAQADIDVLIEGETGTGKELVAHMLHRQSRRSAASLVAVNCAALPHDLAEAELFGSNLVDRATGKLGQAGRIRGAHRGT
LFLDEIDTMHAAVQAKLLRVIEEREVQPLGASAPEPVDLRVVAATKTDLMDAVRAGDFREDLYYRLHVVKLRLPPLRERR
SDIPQTFAFFLDEAASKMGLTGFQIDDATRRHLVEHDWPGNVRELKNFAYSVVLDLPSDPARSAMPAAAPLAERLRRFEA
TIIEDTLAQTRGNIGETMAQLGVPRKTLYDKMAKLGIDPKRFRG

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=466, Percent_Identity=37.9828326180258, Blast_Score=287, Evalue=1e-78,
Organism=Escherichia coli, GI1788550, Length=458, Percent_Identity=37.3362445414847, Blast_Score=275, Evalue=3e-75,
Organism=Escherichia coli, GI1790437, Length=445, Percent_Identity=39.5505617977528, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1788905, Length=453, Percent_Identity=36.644591611479, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1789233, Length=249, Percent_Identity=43.3734939759036, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1786524, Length=261, Percent_Identity=42.5287356321839, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1789087, Length=329, Percent_Identity=39.209726443769, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI87082117, Length=322, Percent_Identity=39.7515527950311, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI87082152, Length=241, Percent_Identity=43.1535269709544, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87081872, Length=224, Percent_Identity=42.8571428571429, Blast_Score=162, Evalue=5e-41,
Organism=Escherichia coli, GI1787583, Length=336, Percent_Identity=33.3333333333333, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI87081858, Length=314, Percent_Identity=35.9872611464968, Blast_Score=144, Evalue=8e-36,
Organism=Escherichia coli, GI1789828, Length=235, Percent_Identity=33.1914893617021, Blast_Score=111, Evalue=9e-26,
Organism=Escherichia coli, GI87082012, Length=136, Percent_Identity=31.6176470588235, Blast_Score=62, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 48478; Mature: 48347

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4 ; PS00758 ARGE_DAPE_CPG2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFFTAEQTILFVDDDAALREANAQTLDLAGLAVETFADAQSVLPRIAADFAGIVITDIR
CCCEECCCEEEEECCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
MPGMDGLELRAAIHAIDPDIPVVLITGHADVTMAVRALHDGAFDFLAKPFAADHLVGVAR
CCCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHCCHHHHHHHHHHH
RALAHRALILDNRRLTAAAAAIDSPLIGESPAMVRLRETIAQLAQADIDVLIEGETGTGK
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHH
ELVAHMLHRQSRRSAASLVAVNCAALPHDLAEAELFGSNLVDRATGKLGQAGRIRGAHRG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCC
TLFLDEIDTMHAAVQAKLLRVIEEREVQPLGASAPEPVDLRVVAATKTDLMDAVRAGDFR
CEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCHH
EDLYYRLHVVKLRLPPLRERRSDIPQTFAFFLDEAASKMGLTGFQIDDATRRHLVEHDWP
HHHHHEEEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHCCCCC
GNVRELKNFAYSVVLDLPSDPARSAMPAAAPLAERLRRFEATIIEDTLAQTRGNIGETMA
CCHHHHHHHHEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
QLGVPRKTLYDKMAKLGIDPKRFRG
HHCCCHHHHHHHHHHHCCCHHHCCC
>Mature Secondary Structure 
SFFTAEQTILFVDDDAALREANAQTLDLAGLAVETFADAQSVLPRIAADFAGIVITDIR
CCEECCCEEEEECCCHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEE
MPGMDGLELRAAIHAIDPDIPVVLITGHADVTMAVRALHDGAFDFLAKPFAADHLVGVAR
CCCCCCHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHCCHHHHHHCCHHHHHHHHHHH
RALAHRALILDNRRLTAAAAAIDSPLIGESPAMVRLRETIAQLAQADIDVLIEGETGTGK
HHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCHH
ELVAHMLHRQSRRSAASLVAVNCAALPHDLAEAELFGSNLVDRATGKLGQAGRIRGAHRG
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCCCCCCCCCCCCC
TLFLDEIDTMHAAVQAKLLRVIEEREVQPLGASAPEPVDLRVVAATKTDLMDAVRAGDFR
CEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCHH
EDLYYRLHVVKLRLPPLRERRSDIPQTFAFFLDEAASKMGLTGFQIDDATRRHLVEHDWP
HHHHHEEEEEEEECCCHHHHHHCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHCCCCC
GNVRELKNFAYSVVLDLPSDPARSAMPAAAPLAERLRRFEATIIEDTLAQTRGNIGETMA
CCHHHHHHHHEEEEEECCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
QLGVPRKTLYDKMAKLGIDPKRFRG
HHCCCHHHHHHHHHHHCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]