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The map label for this gene is pgk

Identifier: 103486804

GI number: 103486804

Start: 1375616

End: 1376812

Strand: Reverse

Name: pgk

Synonym: Sala_1318

Alternate gene names: 103486804

Gene position: 1376812-1375616 (Counterclockwise)

Preceding gene: 103486805

Following gene: 103486803

Centisome position: 41.16

GC content: 66.83

Gene sequence:

>1197_bases
ATGACCGCGTTCAAGACCCTCGACGATATCGGTGACGTCACCGGCAAGCGCGTCCTCGTGCGCGTCGATCTCAACGTCCC
GATGATCGATGGCGCGGTCAGCGACGCGACGCGCATCCAGGCGGCGATGCCGACGATCCGCGAATTGTCGGACAAGGGCG
CGATTGTTCTGCTGCTCGCGCATTTCGGGCGGCCGAAGGGGGCGAAGAACCCGACGCAGTCATTGAGCCTCGTCATGGGC
GGCGTCGAGGATGTGCTGGGCCATTCGGTGATGTTCATTCCCGAAGCGGTCGGCGAGGGCGCGAAGGCGGGCGTTGCCGT
GCTCGCGCCGGGCGATGTCGCGGTGCTCGAGAACACGCGCTTCTATCCCGGCGAGGAGCAGAACGACCCGGCCTTTGCCG
ATGCGCTCGCTGAAATCGGCGACCTGTATGTCAATGACGCCTTTTCGGCCGCGCACCGCGCGCATGGCTCGACCGAAGGC
ATCGCCCGGCGGCTTCCGGCCTATGCAGGGCGTGCGATGGAGGCCGAGCTGAAGGCGCTTGACGCCGCGCTCGGCAATCC
GGTGCAGCCGGTCGCGGCGGTCGTCGGCGGCGCCAAGGTGTCGAGCAAGATCGATGTGCTGAGGAACCTCGTCGGAAAGG
TCGATCACCTCATCATCGGCGGAGGCATGGCGAACACCTTTCTTGCCGCGCGCGGCGTCGACGTCGGCAGGTCGCTGTGC
GAGCATGAGCTGGTCGATACCGCCAATGCGATTTTCGACGCCGCCGACGCCGCGGGCTGCACCATCCATCTGCCCTATGA
CGTCGTGGTGGCAAAAGAGTTTGCGCCGAACCCGCCGACGCGCACCGTCAACGTCCATGAGGTCGCCGCCGACGAGATGA
TCCTCGACGTCGGGCCCGCCGCGATCGAAGCCCTCGCGGACGTACTCAAAAACTGTCGCACGCTGGTGTGGAACGGTCCG
CTCGGCGCGTTCGAGACGCCACCCTTCGACACCGCGACCGTTGCGCTGGCCAGGACCGCTGCCGCGTTGACGCGCGAGGG
ATCGCTCACCTCGGTCGCGGGCGGTGGCGATACCGTCGCGGCGCTCAATCATGCGGGGGTGGCCGGCGACTTCACCTTTG
TTTCGACCGCCGGCGGAGCCTTCCTCGAATGGATGGAAGGCAAGCCGCTGCCGGGCGTGGACGCCCTCAAAGCCTGA

Upstream 100 bases:

>100_bases
CGACGCTATCAGATGGCGGGATGGTGGCGGGTGACGAAAGCGCAGGAGATGGGCCCCCGCCTTCGCGGGGGCACAGCGTC
GGGCAATGGAGTATAAACGG

Downstream 100 bases:

>100_bases
GTTTATCGCGCAATTGCATCCCGCGCGGCGGGACGCTATGCGCGCGGCCACATACGTATGCAATCAGTCGGAGACCTTAT
GACCACCGCCAATGCCGAGA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MTAFKTLDDIGDVTGKRVLVRVDLNVPMIDGAVSDATRIQAAMPTIRELSDKGAIVLLLAHFGRPKGAKNPTQSLSLVMG
GVEDVLGHSVMFIPEAVGEGAKAGVAVLAPGDVAVLENTRFYPGEEQNDPAFADALAEIGDLYVNDAFSAAHRAHGSTEG
IARRLPAYAGRAMEAELKALDAALGNPVQPVAAVVGGAKVSSKIDVLRNLVGKVDHLIIGGGMANTFLAARGVDVGRSLC
EHELVDTANAIFDAADAAGCTIHLPYDVVVAKEFAPNPPTRTVNVHEVAADEMILDVGPAAIEALADVLKNCRTLVWNGP
LGAFETPPFDTATVALARTAAALTREGSLTSVAGGGDTVAALNHAGVAGDFTFVSTAGGAFLEWMEGKPLPGVDALKA

Sequences:

>Translated_398_residues
MTAFKTLDDIGDVTGKRVLVRVDLNVPMIDGAVSDATRIQAAMPTIRELSDKGAIVLLLAHFGRPKGAKNPTQSLSLVMG
GVEDVLGHSVMFIPEAVGEGAKAGVAVLAPGDVAVLENTRFYPGEEQNDPAFADALAEIGDLYVNDAFSAAHRAHGSTEG
IARRLPAYAGRAMEAELKALDAALGNPVQPVAAVVGGAKVSSKIDVLRNLVGKVDHLIIGGGMANTFLAARGVDVGRSLC
EHELVDTANAIFDAADAAGCTIHLPYDVVVAKEFAPNPPTRTVNVHEVAADEMILDVGPAAIEALADVLKNCRTLVWNGP
LGAFETPPFDTATVALARTAAALTREGSLTSVAGGGDTVAALNHAGVAGDFTFVSTAGGAFLEWMEGKPLPGVDALKA
>Mature_397_residues
TAFKTLDDIGDVTGKRVLVRVDLNVPMIDGAVSDATRIQAAMPTIRELSDKGAIVLLLAHFGRPKGAKNPTQSLSLVMGG
VEDVLGHSVMFIPEAVGEGAKAGVAVLAPGDVAVLENTRFYPGEEQNDPAFADALAEIGDLYVNDAFSAAHRAHGSTEGI
ARRLPAYAGRAMEAELKALDAALGNPVQPVAAVVGGAKVSSKIDVLRNLVGKVDHLIIGGGMANTFLAARGVDVGRSLCE
HELVDTANAIFDAADAAGCTIHLPYDVVVAKEFAPNPPTRTVNVHEVAADEMILDVGPAAIEALADVLKNCRTLVWNGPL
GAFETPPFDTATVALARTAAALTREGSLTSVAGGGDTVAALNHAGVAGDFTFVSTAGGAFLEWMEGKPLPGVDALKA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family

Homologues:

Organism=Homo sapiens, GI31543397, Length=411, Percent_Identity=40.8759124087591, Blast_Score=261, Evalue=6e-70,
Organism=Homo sapiens, GI4505763, Length=411, Percent_Identity=40.1459854014599, Blast_Score=258, Evalue=7e-69,
Organism=Escherichia coli, GI1789294, Length=388, Percent_Identity=44.5876288659794, Blast_Score=301, Evalue=4e-83,
Organism=Caenorhabditis elegans, GI17508823, Length=403, Percent_Identity=42.1836228287841, Blast_Score=291, Evalue=3e-79,
Organism=Saccharomyces cerevisiae, GI6319857, Length=400, Percent_Identity=38.25, Blast_Score=243, Evalue=4e-65,
Organism=Drosophila melanogaster, GI17136394, Length=405, Percent_Identity=40.4938271604938, Blast_Score=262, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24581203, Length=404, Percent_Identity=39.1089108910891, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): PGK_SPHAL (Q1GTJ0)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616365.1
- ProteinModelPortal:   Q1GTJ0
- SMR:   Q1GTJ0
- GeneID:   4082242
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_1318
- NMPDR:   fig|317655.9.peg.1253
- HOGENOM:   HBG453500
- OMA:   DCIGDEV
- ProtClustDB:   PRK00073
- BioCyc:   SALA317655:SALA_1318-MONOMER
- GO:   GO:0005737
- GO:   GO:0006096
- HAMAP:   MF_00145
- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824
- Gene3D:   G3DSA:3.40.50.1270
- Gene3D:   G3DSA:3.40.50.1260
- PANTHER:   PTHR11406
- PIRSF:   PIRSF000724
- PRINTS:   PR00477

Pfam domain/function: PF00162 PGK; SSF53748 PGK

EC number: =2.7.2.3

Molecular weight: Translated: 40996; Mature: 40865

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: BINDING 38-38 BINDING 120-120 BINDING 153-153 BINDING 203-203 BINDING 325-325

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAFKTLDDIGDVTGKRVLVRVDLNVPMIDGAVSDATRIQAAMPTIRELSDKGAIVLLLA
CCCHHHHHHHHCCCCCEEEEEEECCCCEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
HFGRPKGAKNPTQSLSLVMGGVEDVLGHSVMFIPEAVGEGAKAGVAVLAPGDVAVLENTR
CCCCCCCCCCHHHHHHHHHCCHHHHHCCCEEEEEHHHCCCCCCCEEEECCCCEEEEECCC
FYPGEEQNDPAFADALAEIGDLYVNDAFSAAHRAHGSTEGIARRLPAYAGRAMEAELKAL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHH
DAALGNPVQPVAAVVGGAKVSSKIDVLRNLVGKVDHLIIGGGMANTFLAARGVDVGRSLC
HHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHCCCHHHHHHH
EHELVDTANAIFDAADAAGCTIHLPYDVVVAKEFAPNPPTRTVNVHEVAADEMILDVGPA
HHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCCCCCCCCCEEEHHHHHHHHHEEECCHH
AIEALADVLKNCRTLVWNGPLGAFETPPFDTATVALARTAAALTREGSLTSVAGGGDTVA
HHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEE
ALNHAGVAGDFTFVSTAGGAFLEWMEGKPLPGVDALKA
EHHCCCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHCC
>Mature Secondary Structure 
TAFKTLDDIGDVTGKRVLVRVDLNVPMIDGAVSDATRIQAAMPTIRELSDKGAIVLLLA
CCHHHHHHHHCCCCCEEEEEEECCCCEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEE
HFGRPKGAKNPTQSLSLVMGGVEDVLGHSVMFIPEAVGEGAKAGVAVLAPGDVAVLENTR
CCCCCCCCCCHHHHHHHHHCCHHHHHCCCEEEEEHHHCCCCCCCEEEECCCCEEEEECCC
FYPGEEQNDPAFADALAEIGDLYVNDAFSAAHRAHGSTEGIARRLPAYAGRAMEAELKAL
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHH
DAALGNPVQPVAAVVGGAKVSSKIDVLRNLVGKVDHLIIGGGMANTFLAARGVDVGRSLC
HHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHEEEECCCHHHHHHHHCCCHHHHHHH
EHELVDTANAIFDAADAAGCTIHLPYDVVVAKEFAPNPPTRTVNVHEVAADEMILDVGPA
HHHHHHHHHHHHHCCCCCCCEEECCCEEEEECCCCCCCCCCEEEHHHHHHHHHEEECCHH
AIEALADVLKNCRTLVWNGPLGAFETPPFDTATVALARTAAALTREGSLTSVAGGGDTVA
HHHHHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCCCEE
ALNHAGVAGDFTFVSTAGGAFLEWMEGKPLPGVDALKA
EHHCCCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA