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The map label for this gene is pdhC [H]

Identifier: 103486722

GI number: 103486722

Start: 1283738

End: 1285048

Strand: Direct

Name: pdhC [H]

Synonym: Sala_1235

Alternate gene names: 103486722

Gene position: 1283738-1285048 (Clockwise)

Preceding gene: 103486720

Following gene: 103486723

Centisome position: 38.38

GC content: 66.97

Gene sequence:

>1311_bases
ATGCCAATCGAACTCAAGATGCCCGCTCTCTCGCCGACGATGGAGGAGGGCACGCTCGCCAAATGGCTTGTGAAGGAGGG
CGATGAGGTCAAGTCGGGCGACCTGCTCGCCGAGATCGAAACCGACAAGGCGACAATGGAGTTCGAAGCGGTCGATGAGG
GTGTGATCAGCCAGATACTCGTCGCCGAGGGGACCGACGGCGTGAAGGTCGGCACGGTGATCGCGGTGATCGCGGGCGAG
GGGGAGGATGCGGGCGAAGCGAAGGCGACGCCTGCTGCTGCGCCTGCGCCGGTTCCGGCAAAAGACGTCGCCCCAGCGGA
AGCTGGGGCCGCTACCGTCAGTGCACCTCCGCCAGCGGTCCTAGCTTCCGCTGGGACGACGAATGTGGGCGACCGCATCA
AAGCCAGCCCGCTCGCCAGGCGCCTCGCTGCCGAGCAAGGCATCGACCTCAAAAAGCTGACCGGCACCGGCCCCGGCGGC
CGCATCGTCAAGGCCGACCTTGAAGGCGCGCCCACAGGCGCCGCTGCATCCACTGCCGTCGCCCCCGCGCAGGCGGGGGC
CGCTGTCGGCACGGCGCCCGCTGCCGCACCGGAACCGGCCGGCCCGATCCCCGATTTCGGCATCCCGCATGAGGATGAAA
AGCTGTCGGGGATGCGCAAGACGATCGCGCGCCGCCTGAGCCAGTCGATGCAGGACGCGCCGCACATCTACCTCACCGTC
GACATCCGCCTCGACGCGCTGCTCAAGCTCCGCGGCGAGCTTAACGCGAGCCTGGAGAGCCGCGGGGTCAAGCTGAGCGT
CAACGACATGCTGATCAAGGCGCTCGCGGTCGCGCTCGAGCGCGTCCCGCAGTGCAACGTCAGCTTTGGCGGCGACGTGA
TGCGCTTTTACAAGCGCGCCGACATTTCGGTCGCGGTCAGCATCCCCGGCGGCCTCATCACCCCGATCATCACCGATGCG
GGGGCCAAGTCGCTGTCGAAAATCTCGACCGAAATGGCCGAGCTCGCGGGCCGCGCCAAGGAAGGCAAGCTGCAACCGCA
CGAATATCAGGGCGGCACCGCCAGCATCTCGAACATGGGCATGATGGGGATCAAGCAGTTCACCGCGGTGATCAACCCGC
CGCAGGCGATGATCATGGCGATCGGCGCGGGCGAAAAGCGGCCCTATGTCGTCGACGATGCGCTGGCGATCGCGACGGTC
ATGTCGGCGACCGGCAGCTTCGACCACCGCGCGATCGACGGGGCGGACGGGGCGCTCTTGATGAAGACGTTCAAGGAACT
GGTGGAAAGCCCGCTGGGGCTGGTGGCGTAA

Upstream 100 bases:

>100_bases
AAGTTTGGCCCGCTGGACCTTGACCGGCGCACGGCTTATGGCGAGGAAGAATCCGTAGCGGCAGGCACGGCCTGCAAGAC
TGACAGGGACACGCCTAGAC

Downstream 100 bases:

>100_bases
GATGCCCGCGAGGGGCCAAAGCCCCTCTCCCCTTGCGGGAGAGGGGTTGGGGAGTGGGGTCCGCGCCGCGGCAGCGGCGC
ACAAGACTCTCTCCGAAGTT

Product: dihydrolipoamide acetyltransferase, long form

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MPIELKMPALSPTMEEGTLAKWLVKEGDEVKSGDLLAEIETDKATMEFEAVDEGVISQILVAEGTDGVKVGTVIAVIAGE
GEDAGEAKATPAAAPAPVPAKDVAPAEAGAATVSAPPPAVLASAGTTNVGDRIKASPLARRLAAEQGIDLKKLTGTGPGG
RIVKADLEGAPTGAAASTAVAPAQAGAAVGTAPAAAPEPAGPIPDFGIPHEDEKLSGMRKTIARRLSQSMQDAPHIYLTV
DIRLDALLKLRGELNASLESRGVKLSVNDMLIKALAVALERVPQCNVSFGGDVMRFYKRADISVAVSIPGGLITPIITDA
GAKSLSKISTEMAELAGRAKEGKLQPHEYQGGTASISNMGMMGIKQFTAVINPPQAMIMAIGAGEKRPYVVDDALAIATV
MSATGSFDHRAIDGADGALLMKTFKELVESPLGLVA

Sequences:

>Translated_436_residues
MPIELKMPALSPTMEEGTLAKWLVKEGDEVKSGDLLAEIETDKATMEFEAVDEGVISQILVAEGTDGVKVGTVIAVIAGE
GEDAGEAKATPAAAPAPVPAKDVAPAEAGAATVSAPPPAVLASAGTTNVGDRIKASPLARRLAAEQGIDLKKLTGTGPGG
RIVKADLEGAPTGAAASTAVAPAQAGAAVGTAPAAAPEPAGPIPDFGIPHEDEKLSGMRKTIARRLSQSMQDAPHIYLTV
DIRLDALLKLRGELNASLESRGVKLSVNDMLIKALAVALERVPQCNVSFGGDVMRFYKRADISVAVSIPGGLITPIITDA
GAKSLSKISTEMAELAGRAKEGKLQPHEYQGGTASISNMGMMGIKQFTAVINPPQAMIMAIGAGEKRPYVVDDALAIATV
MSATGSFDHRAIDGADGALLMKTFKELVESPLGLVA
>Mature_435_residues
PIELKMPALSPTMEEGTLAKWLVKEGDEVKSGDLLAEIETDKATMEFEAVDEGVISQILVAEGTDGVKVGTVIAVIAGEG
EDAGEAKATPAAAPAPVPAKDVAPAEAGAATVSAPPPAVLASAGTTNVGDRIKASPLARRLAAEQGIDLKKLTGTGPGGR
IVKADLEGAPTGAAASTAVAPAQAGAAVGTAPAAAPEPAGPIPDFGIPHEDEKLSGMRKTIARRLSQSMQDAPHIYLTVD
IRLDALLKLRGELNASLESRGVKLSVNDMLIKALAVALERVPQCNVSFGGDVMRFYKRADISVAVSIPGGLITPIITDAG
AKSLSKISTEMAELAGRAKEGKLQPHEYQGGTASISNMGMMGIKQFTAVINPPQAMIMAIGAGEKRPYVVDDALAIATVM
SATGSFDHRAIDGADGALLMKTFKELVESPLGLVA

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=446, Percent_Identity=42.8251121076233, Blast_Score=323, Evalue=2e-88,
Organism=Homo sapiens, GI203098753, Length=461, Percent_Identity=37.3101952277657, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI203098816, Length=461, Percent_Identity=37.3101952277657, Blast_Score=265, Evalue=4e-71,
Organism=Homo sapiens, GI110671329, Length=441, Percent_Identity=27.2108843537415, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI260898739, Length=159, Percent_Identity=42.1383647798742, Blast_Score=122, Evalue=5e-28,
Organism=Homo sapiens, GI19923748, Length=247, Percent_Identity=30.3643724696356, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786946, Length=434, Percent_Identity=30.184331797235, Blast_Score=178, Evalue=5e-46,
Organism=Escherichia coli, GI1786305, Length=412, Percent_Identity=33.252427184466, Blast_Score=158, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=444, Percent_Identity=43.9189189189189, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=36.6013071895425, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17537937, Length=443, Percent_Identity=27.313769751693, Blast_Score=152, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI25146366, Length=441, Percent_Identity=29.9319727891156, Blast_Score=151, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6324258, Length=452, Percent_Identity=41.8141592920354, Blast_Score=299, Evalue=6e-82,
Organism=Saccharomyces cerevisiae, GI6320352, Length=436, Percent_Identity=26.605504587156, Blast_Score=144, Evalue=3e-35,
Organism=Saccharomyces cerevisiae, GI6321632, Length=174, Percent_Identity=31.6091954022989, Blast_Score=76, Evalue=9e-15,
Organism=Drosophila melanogaster, GI20129315, Length=450, Percent_Identity=38.2222222222222, Blast_Score=264, Evalue=7e-71,
Organism=Drosophila melanogaster, GI24582497, Length=439, Percent_Identity=37.5854214123007, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI18859875, Length=428, Percent_Identity=29.2056074766355, Blast_Score=151, Evalue=8e-37,
Organism=Drosophila melanogaster, GI24645909, Length=224, Percent_Identity=30.8035714285714, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006257
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 44645; Mature: 44514

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIELKMPALSPTMEEGTLAKWLVKEGDEVKSGDLLAEIETDKATMEFEAVDEGVISQIL
CCCEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHH
VAEGTDGVKVGTVIAVIAGEGEDAGEAKATPAAAPAPVPAKDVAPAEAGAATVSAPPPAV
HCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCE
LASAGTTNVGDRIKASPLARRLAAEQGIDLKKLTGTGPGGRIVKADLEGAPTGAAASTAV
EECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCCCCHHHC
APAQAGAAVGTAPAAAPEPAGPIPDFGIPHEDEKLSGMRKTIARRLSQSMQDAPHIYLTV
CCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DIRLDALLKLRGELNASLESRGVKLSVNDMLIKALAVALERVPQCNVSFGGDVMRFYKRA
EEEHHHHHHHHHHHCCCHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
DISVAVSIPGGLITPIITDAGAKSLSKISTEMAELAGRAKEGKLQPHEYQGGTASISNMG
CCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCC
MMGIKQFTAVINPPQAMIMAIGAGEKRPYVVDDALAIATVMSATGSFDHRAIDGADGALL
HHHHHHHHHHCCCCHHEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
MKTFKELVESPLGLVA
HHHHHHHHHCCCCCCC
>Mature Secondary Structure 
PIELKMPALSPTMEEGTLAKWLVKEGDEVKSGDLLAEIETDKATMEFEAVDEGVISQIL
CCEEECCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHH
VAEGTDGVKVGTVIAVIAGEGEDAGEAKATPAAAPAPVPAKDVAPAEAGAATVSAPPPAV
HCCCCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCE
LASAGTTNVGDRIKASPLARRLAAEQGIDLKKLTGTGPGGRIVKADLEGAPTGAAASTAV
EECCCCCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEECCCCCCCCCCHHHC
APAQAGAAVGTAPAAAPEPAGPIPDFGIPHEDEKLSGMRKTIARRLSQSMQDAPHIYLTV
CCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEE
DIRLDALLKLRGELNASLESRGVKLSVNDMLIKALAVALERVPQCNVSFGGDVMRFYKRA
EEEHHHHHHHHHHHCCCHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHC
DISVAVSIPGGLITPIITDAGAKSLSKISTEMAELAGRAKEGKLQPHEYQGGTASISNMG
CCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHCC
MMGIKQFTAVINPPQAMIMAIGAGEKRPYVVDDALAIATVMSATGSFDHRAIDGADGALL
HHHHHHHHHHCCCCHHEEEEECCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCHHH
MKTFKELVESPLGLVA
HHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]