Click here to switch to the map view.

The map label for this gene is pcbC [H]

Identifier: 103486529

GI number: 103486529

Start: 1073289

End: 1074218

Strand: Reverse

Name: pcbC [H]

Synonym: Sala_1040

Alternate gene names: 103486529

Gene position: 1074218-1073289 (Counterclockwise)

Preceding gene: 103486531

Following gene: 103486527

Centisome position: 32.11

GC content: 63.76

Gene sequence:

>930_bases
ATGACCGCTGCCGTCCCCGTCATTTCCCTGGCCCTTTCCGCCGACCGGTTTGCCCGTGATTTCGGAGGATCGTTCGAACG
CTTCGGCTTTGCGATGATCACCGATCACGGCATTGACCTCACGCTGATCGACGAGGCCTGGACCAAGGCCAAGGCGTTTT
TCGCGCTGCCGGAAGCAGTCAAGCGTGCCTATCATATCCCCGGCGGCGGCGGCGCGCGCGGCTACACACCCTTCGGAACC
GAAATCGCCAAGGGCGCGAAAGAGGTCGACCTGAAAGAGTTCTGGCATGTCGGGCGCGACCTGCCGCCCGGCCACCCGCT
TGCCGCGCAACAGCCGAACAATGTCTGGCCGGCAGAGATCGAAGGGTTTCGCGCCGTTTTTGAGAGGCTGTTTGCCGAGT
TCGACCGGGTGGGCGGCCAGCTTTTGTCGGGCATCGCACGCTATCTCGGCCTCGCCCCCGATTTCTTCGATGACACGGTG
AAGGACGGCAACAGCGTCATGCGCCTGCTTCACTATCCGCCGGTCGAGGCGCCCGCCAAAGGCATCCGCGCCGAAGCGCA
TGAGGACATCAACACCATCACCCTCCTGCTCGGCGCCGAGGAAGCGGGGCTCGAGATTCTCGACAAGGACGGCAGTTGGC
TTCCCGTTTCGCCGCCGCCGGGCGCGATGGCGGTCAATGTCGGCGACATGCTTCAGCGGCTGACGAACAACCGCCTCCCC
TCGACGACGCACCGCGTCCGCAACCCCGACGAAGGCCGCGCCGGCGTCGCGCGCTATTCGATGCCCTTCTTCCTGCATTT
CCGTTCGGACTATCTGATCGAGACGCTGGACAACTGCATCGACGCCGATCACCCCGACCTCTACCCGACACCGATCACCG
CCGACGATTATCTGCAGGAACGCCTGCGCGAAATCGGGCTGAAGAAATAG

Upstream 100 bases:

>100_bases
CGAAACCGCCTTTTTCCCTTTAACCCGCGCGTCGCAGCGCCTAAATCGCGAGTCATGACGCGCCGCCTGGCGCCGCCCCT
TGTCAAATGGATTTCATTGC

Downstream 100 bases:

>100_bases
GCGGTTATTCCCTTAGAGCGCGATAGAGCGGCGTGCTGTCGTCATTGCGAGCGAAGCGAAGCAATGACGCCGAGGGAAGA
ATGAGGTGAAGGCGGGCAAC

Product: 2OG-Fe(II) oxygenase

Products: NA

Alternate protein names: IPNS [H]

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTAAVPVISLALSADRFARDFGGSFERFGFAMITDHGIDLTLIDEAWTKAKAFFALPEAVKRAYHIPGGGGARGYTPFGT
EIAKGAKEVDLKEFWHVGRDLPPGHPLAAQQPNNVWPAEIEGFRAVFERLFAEFDRVGGQLLSGIARYLGLAPDFFDDTV
KDGNSVMRLLHYPPVEAPAKGIRAEAHEDINTITLLLGAEEAGLEILDKDGSWLPVSPPPGAMAVNVGDMLQRLTNNRLP
STTHRVRNPDEGRAGVARYSMPFFLHFRSDYLIETLDNCIDADHPDLYPTPITADDYLQERLREIGLKK

Sequences:

>Translated_309_residues
MTAAVPVISLALSADRFARDFGGSFERFGFAMITDHGIDLTLIDEAWTKAKAFFALPEAVKRAYHIPGGGGARGYTPFGT
EIAKGAKEVDLKEFWHVGRDLPPGHPLAAQQPNNVWPAEIEGFRAVFERLFAEFDRVGGQLLSGIARYLGLAPDFFDDTV
KDGNSVMRLLHYPPVEAPAKGIRAEAHEDINTITLLLGAEEAGLEILDKDGSWLPVSPPPGAMAVNVGDMLQRLTNNRLP
STTHRVRNPDEGRAGVARYSMPFFLHFRSDYLIETLDNCIDADHPDLYPTPITADDYLQERLREIGLKK
>Mature_308_residues
TAAVPVISLALSADRFARDFGGSFERFGFAMITDHGIDLTLIDEAWTKAKAFFALPEAVKRAYHIPGGGGARGYTPFGTE
IAKGAKEVDLKEFWHVGRDLPPGHPLAAQQPNNVWPAEIEGFRAVFERLFAEFDRVGGQLLSGIARYLGLAPDFFDDTVK
DGNSVMRLLHYPPVEAPAKGIRAEAHEDINTITLLLGAEEAGLEILDKDGSWLPVSPPPGAMAVNVGDMLQRLTNNRLPS
TTHRVRNPDEGRAGVARYSMPFFLHFRSDYLIETLDNCIDADHPDLYPTPITADDYLQERLREIGLKK

Specific function: Removes, in the presence of oxygen, 4 hydrogen atoms from delta-L-(alpha-aminoadipyl)-L-cysteinyl-D-valine (ACV) to form the azetidinone and thiazolidine rings of isopenicillin [H]

COG id: COG3491

COG function: function code R; Isopenicillin N synthase and related dioxygenases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe2OG dioxygenase domain [H]

Homologues:

Organism=Drosophila melanogaster, GI85816264, Length=291, Percent_Identity=28.1786941580756, Blast_Score=94, Evalue=9e-20,
Organism=Drosophila melanogaster, GI28572124, Length=290, Percent_Identity=27.2413793103448, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002057
- InterPro:   IPR002283
- InterPro:   IPR005123 [H]

Pfam domain/function: PF03171 2OG-FeII_Oxy [H]

EC number: =1.21.3.1 [H]

Molecular weight: Translated: 34084; Mature: 33953

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAVPVISLALSADRFARDFGGSFERFGFAMITDHGIDLTLIDEAWTKAKAFFALPEAV
CCCCHHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHH
KRAYHIPGGGGARGYTPFGTEIAKGAKEVDLKEFWHVGRDLPPGHPLAAQQPNNVWPAEI
HHHCCCCCCCCCCCCCCCCHHHHCCCHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
EGFRAVFERLFAEFDRVGGQLLSGIARYLGLAPDFFDDTVKDGNSVMRLLHYPPVEAPAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHCCCCCCCHH
GIRAEAHEDINTITLLLGAEEAGLEILDKDGSWLPVSPPPGAMAVNVGDMLQRLTNNRLP
HCHHHHHCCCCEEEEEEECCCCCCEEEECCCCEEECCCCCCCEEECHHHHHHHHHCCCCC
STTHRVRNPDEGRAGVARYSMPFFLHFRSDYLIETLDNCIDADHPDLYPTPITADDYLQE
CHHHHCCCCCCCCCCHHHHCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RLREIGLKK
HHHHCCCCC
>Mature Secondary Structure 
TAAVPVISLALSADRFARDFGGSFERFGFAMITDHGIDLTLIDEAWTKAKAFFALPEAV
CCCHHHHHHHHHHHHHHHHHCCCHHHCCEEEEECCCCCEEEECHHHHHHHHHHHHHHHH
KRAYHIPGGGGARGYTPFGTEIAKGAKEVDLKEFWHVGRDLPPGHPLAAQQPNNVWPAEI
HHHCCCCCCCCCCCCCCCCHHHHCCCHHCCHHHHHHHCCCCCCCCCCCCCCCCCCCCHHH
EGFRAVFERLFAEFDRVGGQLLSGIARYLGLAPDFFDDTVKDGNSVMRLLHYPPVEAPAK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHHHHHHCCCCCCCHH
GIRAEAHEDINTITLLLGAEEAGLEILDKDGSWLPVSPPPGAMAVNVGDMLQRLTNNRLP
HCHHHHHCCCCEEEEEEECCCCCCEEEECCCCEEECCCCCCCEEECHHHHHHHHHCCCCC
STTHRVRNPDEGRAGVARYSMPFFLHFRSDYLIETLDNCIDADHPDLYPTPITADDYLQE
CHHHHCCCCCCCCCCHHHHCCCEEEEECHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
RLREIGLKK
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1901702 [H]