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The map label for this gene is greA

Identifier: 103486475

GI number: 103486475

Start: 1013324

End: 1013800

Strand: Direct

Name: greA

Synonym: Sala_0986

Alternate gene names: 103486475

Gene position: 1013324-1013800 (Clockwise)

Preceding gene: 103486474

Following gene: 103486476

Centisome position: 30.29

GC content: 61.22

Gene sequence:

>477_bases
ATGGCAAGCGTTGAAAAGGTGCCGATGCTGGCAGAAGGCTATGAGAAGCTGACCGCGCAACTTGCGGCGCTGAAAGCCGA
GCGACCGCTGATCGTCGATGCGATCGAGGAAGCGCGCGCGCATGGCGACCTTTCGGAAAATGCCGAATATCACGCCGCCA
AGGAACGGCAGGGCCAGGTCGAAGCGACGATCGGCGACCTTGAGGACAAATTGTCGCGCGCGCAGGTCATCGACCCGACG
ACGCTGTCGGGCGACCGCATCGTCTTCGGCGCGACGGTCACGCTGACCGACGAGGACGACAAGCCGGTCAAATATCAGAT
TGTCGGGCAGGCCGAAGCCGACGCCAAGGCGGGCAAGATCAGCTATAATTCGCCGCTCGGCCGTGCGCTGATCGGCCGTC
GCGTCGACGACGAGATCGAAGTGACCGTGCCGTCGGGCGACAAATATTATCTGGTGACCAAGATCGAATTCATCTGA

Upstream 100 bases:

>100_bases
TTGACGCACCGCTCCACCTCCCCACATCGACGGAAACGGCGGCTCAGCCGCCCGGCGCGCAACCGCCGGGGCGGGATTAT
TGACGAAGGACAACAGGATA

Downstream 100 bases:

>100_bases
TGGGTTGCCGGCGATGAAGCCGCAGGACGCGCCGCTGGTCACGGGCTATGCGCTCGCCTGCGTCATCCTGTTCGTCCTGC
TCTGGATCACGGGCTTTCAG

Product: transcription elongation factor GreA

Products: NA

Alternate protein names: Transcript cleavage factor greA

Number of amino acids: Translated: 158; Mature: 157

Protein sequence:

>158_residues
MASVEKVPMLAEGYEKLTAQLAALKAERPLIVDAIEEARAHGDLSENAEYHAAKERQGQVEATIGDLEDKLSRAQVIDPT
TLSGDRIVFGATVTLTDEDDKPVKYQIVGQAEADAKAGKISYNSPLGRALIGRRVDDEIEVTVPSGDKYYLVTKIEFI

Sequences:

>Translated_158_residues
MASVEKVPMLAEGYEKLTAQLAALKAERPLIVDAIEEARAHGDLSENAEYHAAKERQGQVEATIGDLEDKLSRAQVIDPT
TLSGDRIVFGATVTLTDEDDKPVKYQIVGQAEADAKAGKISYNSPLGRALIGRRVDDEIEVTVPSGDKYYLVTKIEFI
>Mature_157_residues
ASVEKVPMLAEGYEKLTAQLAALKAERPLIVDAIEEARAHGDLSENAEYHAAKERQGQVEATIGDLEDKLSRAQVIDPTT
LSGDRIVFGATVTLTDEDDKPVKYQIVGQAEADAKAGKISYNSPLGRALIGRRVDDEIEVTVPSGDKYYLVTKIEFI

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family

Homologues:

Organism=Escherichia coli, GI87082225, Length=158, Percent_Identity=49.3670886075949, Blast_Score=140, Evalue=3e-35,
Organism=Escherichia coli, GI87082261, Length=155, Percent_Identity=32.258064516129, Blast_Score=86, Evalue=1e-18,

Paralogues:

None

Copy number: 259 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): GREA_SPHAL (Q1GUG9)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_616036.1
- ProteinModelPortal:   Q1GUG9
- SMR:   Q1GUG9
- GeneID:   4081674
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0986
- NMPDR:   fig|317655.9.peg.925
- HOGENOM:   HBG753387
- OMA:   HNEGRIA
- ProtClustDB:   PRK00226
- BioCyc:   SALA317655:SALA_0986-MONOMER
- GO:   GO:0006350
- HAMAP:   MF_00105
- InterPro:   IPR018151
- InterPro:   IPR006359
- InterPro:   IPR001437
- InterPro:   IPR022691
- Gene3D:   G3DSA:1.10.287.180
- Gene3D:   G3DSA:3.10.50.30
- TIGRFAMs:   TIGR01462

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N; SSF46557 GreA_GreB

EC number: NA

Molecular weight: Translated: 17203; Mature: 17071

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS00829 GREAB_1; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASVEKVPMLAEGYEKLTAQLAALKAERPLIVDAIEEARAHGDLSENAEYHAAKERQGQV
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCHHHHHCCCCEE
EATIGDLEDKLSRAQVIDPTTLSGDRIVFGATVTLTDEDDKPVKYQIVGQAEADAKAGKI
EEEHHHHHHHHHHCEECCCCCCCCCEEEEEEEEEEECCCCCCEEEEEEECCCCCCCCCEE
SYNSPLGRALIGRRVDDEIEVTVPSGDKYYLVTKIEFI
ECCCCHHHHHHCCCCCCCEEEEECCCCCEEEEEEEEEC
>Mature Secondary Structure 
ASVEKVPMLAEGYEKLTAQLAALKAERPLIVDAIEEARAHGDLSENAEYHAAKERQGQV
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHCCCCCCCCCHHHHHCCCCEE
EATIGDLEDKLSRAQVIDPTTLSGDRIVFGATVTLTDEDDKPVKYQIVGQAEADAKAGKI
EEEHHHHHHHHHHCEECCCCCCCCCEEEEEEEEEEECCCCCCEEEEEEECCCCCCCCCEE
SYNSPLGRALIGRRVDDEIEVTVPSGDKYYLVTKIEFI
ECCCCHHHHHHCCCCCCCEEEEECCCCCEEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA