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The map label for this gene is carA [H]

Identifier: 103486472

GI number: 103486472

Start: 1008171

End: 1009442

Strand: Direct

Name: carA [H]

Synonym: Sala_0983

Alternate gene names: 103486472

Gene position: 1008171-1009442 (Clockwise)

Preceding gene: 103486462

Following gene: 103486473

Centisome position: 30.14

GC content: 66.75

Gene sequence:

>1272_bases
ATGGCACCAGCCAACCCATCAGTCGCGCCCAAAAGCCAGCCGTCCGGCGCCACCGGCGTCCTCGTCCTTGCCGACGGCAC
CATCCTGTGGGGCGTCGGTTATGGCGCGAGCGGGGCCGCGGCAGGCGAGATTTGTTTCAACACGTCGATGACCGGCTATC
AGGAAATCCTGACCGACCCCAGCTACGCCGGGCAGATCATCACCTTCACCTTTCCGCACATCGGCAATGTCGGCGCGAAC
CCGGAGGATATGGAGCGCAGCGTCCACGGTGCGCTCGGCGCGATCACGCGCGAGCTGCCGACGGCGCCCAGCAATTTTCG
AAGCGTGCAGACCCTGCCCGAATGGATGACGGAGCAGGGGGTGATCGGGCTGGCGGGCATCGACACGCGCGCGCTGACGC
GGCGCATCCGCGATGCAGGCGCGCCGAACGGGGTGATCGCGCACAGCCCGGACGCCAAGTTTGACATTGACGCGCTGCTG
GCGATGGCGCGGGGTTGGCCGGGGCTGGAGGGGCTGGACCTCGCGAAGACCGTCAGCCGGACCGATACGGGTGAGTGGAC
CGGCGGTATTTGGGCTCTCGGTAAGGGTTATCAAACCCCGTTCGCATCGAGCGAAGTCGAGATGCCCATCGACGGCGCAC
GACCTCATGGTGTCTCGACTTCGCTCGACACGAACGGGAATGGGGCGACGGCTCAAGCCCGCCCCCATGTCGTCGCGATC
GACTATGGCGCAAAGGACAATATCTTCCGGAGCCTCGTGAAGGCCGGCGCGCGCGTCACCGTGGTGCCCGCGACCGCGAG
CCTCGACGAGGTGCTGGCGCTGGAGCCCGCGGGGGTGTTCCTGTCGAACGGCCCCGGCGATCCGGCGGCGACGGGCGACT
ATGCGGTGCCGGTGATCCAGGGGCTGCTCGAACGCAATGTCCCGATCTTCGGTATCTGCCTCGGCCACCAGCTGCTCGCG
CTCGCCGCGGGCGCCCGGACGGTGAAGATGCACCAGGGTCACCGCGGCGCGAACCATCCGGTCCAGCGCGTCGGCGGCGA
TTTCGCCGACGGCGTGGTCGAGATCACCAGCATGAACCACGGCTTCGCGGTCGACGCCTCGACCTTGCCCGCGGGCGTGG
TCGAAACGCACAAGAGCCTGTTCGACGGATCGAACTGCGGCATCGCGATCACGGGCAAAAATGCGTTCAGCGTACAATAT
CACCCCGAGGCGAGCCCGGGGCCGCAGGACAGTTTCTATCTGTTCGAGAAGTTTGTGGGCGGGCTTCGTTAA

Upstream 100 bases:

>100_bases
GGCCCTCTCTAGCGGGAAGATTCCAAATCCGATAGCTTGTGAATCACTTTTTTGGCGCCTAATTGGTCGGCCGTTTAGCC
CCCCGTCCGGAGCATGTTAA

Downstream 100 bases:

>100_bases
TGAGTTCAATTGGCTCACCTTCCTCCTTGCCCGCTGGGGAGGGTTCGGCTGAGGAGCCGGATGCCTTGCCTCCCCGCAAT
TCAATCGTGACCCAAGCTGG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MAPANPSVAPKSQPSGATGVLVLADGTILWGVGYGASGAAAGEICFNTSMTGYQEILTDPSYAGQIITFTFPHIGNVGAN
PEDMERSVHGALGAITRELPTAPSNFRSVQTLPEWMTEQGVIGLAGIDTRALTRRIRDAGAPNGVIAHSPDAKFDIDALL
AMARGWPGLEGLDLAKTVSRTDTGEWTGGIWALGKGYQTPFASSEVEMPIDGARPHGVSTSLDTNGNGATAQARPHVVAI
DYGAKDNIFRSLVKAGARVTVVPATASLDEVLALEPAGVFLSNGPGDPAATGDYAVPVIQGLLERNVPIFGICLGHQLLA
LAAGARTVKMHQGHRGANHPVQRVGGDFADGVVEITSMNHGFAVDASTLPAGVVETHKSLFDGSNCGIAITGKNAFSVQY
HPEASPGPQDSFYLFEKFVGGLR

Sequences:

>Translated_423_residues
MAPANPSVAPKSQPSGATGVLVLADGTILWGVGYGASGAAAGEICFNTSMTGYQEILTDPSYAGQIITFTFPHIGNVGAN
PEDMERSVHGALGAITRELPTAPSNFRSVQTLPEWMTEQGVIGLAGIDTRALTRRIRDAGAPNGVIAHSPDAKFDIDALL
AMARGWPGLEGLDLAKTVSRTDTGEWTGGIWALGKGYQTPFASSEVEMPIDGARPHGVSTSLDTNGNGATAQARPHVVAI
DYGAKDNIFRSLVKAGARVTVVPATASLDEVLALEPAGVFLSNGPGDPAATGDYAVPVIQGLLERNVPIFGICLGHQLLA
LAAGARTVKMHQGHRGANHPVQRVGGDFADGVVEITSMNHGFAVDASTLPAGVVETHKSLFDGSNCGIAITGKNAFSVQY
HPEASPGPQDSFYLFEKFVGGLR
>Mature_422_residues
APANPSVAPKSQPSGATGVLVLADGTILWGVGYGASGAAAGEICFNTSMTGYQEILTDPSYAGQIITFTFPHIGNVGANP
EDMERSVHGALGAITRELPTAPSNFRSVQTLPEWMTEQGVIGLAGIDTRALTRRIRDAGAPNGVIAHSPDAKFDIDALLA
MARGWPGLEGLDLAKTVSRTDTGEWTGGIWALGKGYQTPFASSEVEMPIDGARPHGVSTSLDTNGNGATAQARPHVVAID
YGAKDNIFRSLVKAGARVTVVPATASLDEVLALEPAGVFLSNGPGDPAATGDYAVPVIQGLLERNVPIFGICLGHQLLAL
AAGARTVKMHQGHRGANHPVQRVGGDFADGVVEITSMNHGFAVDASTLPAGVVETHKSLFDGSNCGIAITGKNAFSVQYH
PEASPGPQDSFYLFEKFVGGLR

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=418, Percent_Identity=36.8421052631579, Blast_Score=215, Evalue=6e-56,
Organism=Homo sapiens, GI21361331, Length=425, Percent_Identity=32, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI169790915, Length=425, Percent_Identity=32, Blast_Score=178, Evalue=8e-45,
Organism=Escherichia coli, GI1786215, Length=404, Percent_Identity=51.2376237623762, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI193204318, Length=419, Percent_Identity=35.0835322195704, Blast_Score=207, Evalue=1e-53,
Organism=Saccharomyces cerevisiae, GI6322331, Length=437, Percent_Identity=33.1807780320366, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6324878, Length=196, Percent_Identity=44.8979591836735, Blast_Score=146, Evalue=7e-36,
Organism=Drosophila melanogaster, GI45555749, Length=421, Percent_Identity=34.4418052256532, Blast_Score=208, Evalue=5e-54,
Organism=Drosophila melanogaster, GI24642586, Length=421, Percent_Identity=34.4418052256532, Blast_Score=207, Evalue=9e-54,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 43886; Mature: 43755

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPANPSVAPKSQPSGATGVLVLADGTILWGVGYGASGAAAGEICFNTSMTGYQEILTDP
CCCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCEEECCCCCHHHHHHCCC
SYAGQIITFTFPHIGNVGANPEDMERSVHGALGAITRELPTAPSNFRSVQTLPEWMTEQG
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
VIGLAGIDTRALTRRIRDAGAPNGVIAHSPDAKFDIDALLAMARGWPGLEGLDLAKTVSR
CEEEECCCHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHC
TDTGEWTGGIWALGKGYQTPFASSEVEMPIDGARPHGVSTSLDTNGNGATAQARPHVVAI
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
DYGAKDNIFRSLVKAGARVTVVPATASLDEVLALEPAGVFLSNGPGDPAATGDYAVPVIQ
ECCCCHHHHHHHHHCCCEEEEEECCCCCCHHHEECCCCEEEECCCCCCCCCCCCHHHHHH
GLLERNVPIFGICLGHQLLALAAGARTVKMHQGHRGANHPVQRVGGDFADGVVEITSMNH
HHHHCCCCEEEEEHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCCCHHCCEEEEEECCC
GFAVDASTLPAGVVETHKSLFDGSNCGIAITGKNAFSVQYHPEASPGPQDSFYLFEKFVG
CEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHC
GLR
CCC
>Mature Secondary Structure 
APANPSVAPKSQPSGATGVLVLADGTILWGVGYGASGAAAGEICFNTSMTGYQEILTDP
CCCCCCCCCCCCCCCCCEEEEEECCEEEEEECCCCCCCCCCCEEECCCCCHHHHHHCCC
SYAGQIITFTFPHIGNVGANPEDMERSVHGALGAITRELPTAPSNFRSVQTLPEWMTEQG
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
VIGLAGIDTRALTRRIRDAGAPNGVIAHSPDAKFDIDALLAMARGWPGLEGLDLAKTVSR
CEEEECCCHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHC
TDTGEWTGGIWALGKGYQTPFASSEVEMPIDGARPHGVSTSLDTNGNGATAQARPHVVAI
CCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
DYGAKDNIFRSLVKAGARVTVVPATASLDEVLALEPAGVFLSNGPGDPAATGDYAVPVIQ
ECCCCHHHHHHHHHCCCEEEEEECCCCCCHHHEECCCCEEEECCCCCCCCCCCCHHHHHH
GLLERNVPIFGICLGHQLLALAAGARTVKMHQGHRGANHPVQRVGGDFADGVVEITSMNH
HHHHCCCCEEEEEHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHCCCHHCCEEEEEECCC
GFAVDASTLPAGVVETHKSLFDGSNCGIAITGKNAFSVQYHPEASPGPQDSFYLFEKFVG
CEEEECCCCCHHHHHHHHHHHCCCCCCEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHC
GLR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA