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The map label for this gene is rimO

Identifier: 103486211

GI number: 103486211

Start: 724305

End: 725672

Strand: Reverse

Name: rimO

Synonym: Sala_0718

Alternate gene names: 103486211

Gene position: 725672-724305 (Counterclockwise)

Preceding gene: 103486213

Following gene: 103486208

Centisome position: 21.69

GC content: 65.94

Gene sequence:

>1368_bases
ATGACAACCAAGATTCTTCCTTCCCAGCCGAAGGTCGGCATGGTGTCGCTCGGCTGCCCCAAGGCGCTCGTCGACAGCGA
ACGGATTCTGACCAAGCTGCGCGCCGATGGCTATGGCCTTTCGCCCGACTATGCGGGCGCCGATGTCGTGCTCGTCAACA
CCTGCGGCTTCCTCGACTCGGCGAAGGAAGAAAGCCTTGAGGCGATCGGCGAGGCGATGGCCGAAAACGGCCGCGTCATC
GTCACCGGCTGCATGGGCAAGGAGGCCGACGTCATCCGCGCGCGATTCCCGAACGTGCTCGCGGTCACCGGCGCGCATCA
GTACGAACAGGTCGTCGAAGCCGTCCACGACGCCGCGCCGCCGACGCAGGGCCCGTTCGTCGACCTCGTTCCCGAGGGCG
GGCTGAAACTCACCCCGCGCCACTACAGCTATCTGAAGATCTCCGAAGGCTGCAACCACAGCTGCGCCTTCTGCATCATC
CCAGATCTGCGCGGCAAACTCGTCAGCCGCCGTATCGACGCGGTGCTGCGCGAGGCTGAAAAGCTCGTCGCCGCGGGGAC
GAAGGAATTGCTCGTCATCAGCCAGGACACGTCGGCTTACGGCGTCGATATCCGCCACGACCCGCGCCAGTGGCACGGCC
GCGAAATCCGCGCGCACATGACCGACCTCGCGCGCGAACTCGGGCAGCTTCGGACCAGCGAAGGCCGCGCGCCGTGGGTG
CGCCTCCACTATGTCTATCCCTACCCGCACGTCGATGCGGTCATCCCGCTGATGGCCGAGGGACTGCTGACGCCCTATCT
CGACATCCCCTTCCAGCACGCGAGCCCCAGCGTCCTCAAGCGCATGAAGCGCCCCGCGAACGAAGCGAAGGTGCTCGAAC
GGCTGAAGAGCTGGCGCGCCATCGCCCCCGACATCGCGATCCGTTCGAGCTTCGTCGTCGGCTTCCCCGGCGAGACCGAA
GCCGATTTCCAGTATCTCCTCGACTGGCTCGAAGAAGCGCAGCTCGACCGCGTCGGCGCCTTCCGCTTCGAACCCGTCGC
CGGCGCGCAGGCCAACGCCCTGCCCGAGCCGGTCCCCGACGAGGTCAAGGAAGAACGCTATCAGCGCATCATGGCGAAAA
CCGCCGCGATCAGCGCCGCGAAGCTTGAAGCCAAGATCGGCCGCACGCTACCGGTGATCATCGACGAAGTCGGCGAGGCG
GACGAAGACGGTAGCATCGGCGCGACCGGCCGGTCACAGGCCGACGCCCCCGAAATCGACGGCCACGTCTATCTGCGCGA
CGTCGCGGCGACACTGAAAGCCGGCGACATCGTCGATGTCGAAATCGAGGACGCCGACGAGCATGACCTGTTCGGCGTGG
TAACCTGA

Upstream 100 bases:

>100_bases
AGTCCAGATTTGCCATTTGTCCTCCCCTTTTGCCACGCAACATGCTCCGTCTCGCCGCGCGTGACAAGTGGCGCGAAATC
GACTATGCGCGCCGCCGATC

Downstream 100 bases:

>100_bases
ACGCAGACCCGCCGAAAATCGCTGTTCTGCCTGGCGGGGGTCGCATAGCCCTGTGGAATGGCGCCTGCACGCTCCACGAT
ACAGCGAGTTCAGAGTGAAG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTTKILPSQPKVGMVSLGCPKALVDSERILTKLRADGYGLSPDYAGADVVLVNTCGFLDSAKEESLEAIGEAMAENGRVI
VTGCMGKEADVIRARFPNVLAVTGAHQYEQVVEAVHDAAPPTQGPFVDLVPEGGLKLTPRHYSYLKISEGCNHSCAFCII
PDLRGKLVSRRIDAVLREAEKLVAAGTKELLVISQDTSAYGVDIRHDPRQWHGREIRAHMTDLARELGQLRTSEGRAPWV
RLHYVYPYPHVDAVIPLMAEGLLTPYLDIPFQHASPSVLKRMKRPANEAKVLERLKSWRAIAPDIAIRSSFVVGFPGETE
ADFQYLLDWLEEAQLDRVGAFRFEPVAGAQANALPEPVPDEVKEERYQRIMAKTAAISAAKLEAKIGRTLPVIIDEVGEA
DEDGSIGATGRSQADAPEIDGHVYLRDVAATLKAGDIVDVEIEDADEHDLFGVVT

Sequences:

>Translated_455_residues
MTTKILPSQPKVGMVSLGCPKALVDSERILTKLRADGYGLSPDYAGADVVLVNTCGFLDSAKEESLEAIGEAMAENGRVI
VTGCMGKEADVIRARFPNVLAVTGAHQYEQVVEAVHDAAPPTQGPFVDLVPEGGLKLTPRHYSYLKISEGCNHSCAFCII
PDLRGKLVSRRIDAVLREAEKLVAAGTKELLVISQDTSAYGVDIRHDPRQWHGREIRAHMTDLARELGQLRTSEGRAPWV
RLHYVYPYPHVDAVIPLMAEGLLTPYLDIPFQHASPSVLKRMKRPANEAKVLERLKSWRAIAPDIAIRSSFVVGFPGETE
ADFQYLLDWLEEAQLDRVGAFRFEPVAGAQANALPEPVPDEVKEERYQRIMAKTAAISAAKLEAKIGRTLPVIIDEVGEA
DEDGSIGATGRSQADAPEIDGHVYLRDVAATLKAGDIVDVEIEDADEHDLFGVVT
>Mature_454_residues
TTKILPSQPKVGMVSLGCPKALVDSERILTKLRADGYGLSPDYAGADVVLVNTCGFLDSAKEESLEAIGEAMAENGRVIV
TGCMGKEADVIRARFPNVLAVTGAHQYEQVVEAVHDAAPPTQGPFVDLVPEGGLKLTPRHYSYLKISEGCNHSCAFCIIP
DLRGKLVSRRIDAVLREAEKLVAAGTKELLVISQDTSAYGVDIRHDPRQWHGREIRAHMTDLARELGQLRTSEGRAPWVR
LHYVYPYPHVDAVIPLMAEGLLTPYLDIPFQHASPSVLKRMKRPANEAKVLERLKSWRAIAPDIAIRSSFVVGFPGETEA
DFQYLLDWLEEAQLDRVGAFRFEPVAGAQANALPEPVPDEVKEERYQRIMAKTAAISAAKLEAKIGRTLPVIIDEVGEAD
EDGSIGATGRSQADAPEIDGHVYLRDVAATLKAGDIVDVEIEDADEHDLFGVVT

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=408, Percent_Identity=26.7156862745098, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI28872784, Length=492, Percent_Identity=24.1869918699187, Blast_Score=116, Evalue=6e-26,
Organism=Homo sapiens, GI28872782, Length=492, Percent_Identity=24.1869918699187, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1787057, Length=451, Percent_Identity=60.9756097560976, Blast_Score=552, Evalue=1e-158,
Organism=Escherichia coli, GI1786882, Length=468, Percent_Identity=26.2820512820513, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17553146, Length=469, Percent_Identity=23.8805970149254, Blast_Score=108, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI71996771, Length=324, Percent_Identity=24.0740740740741, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19922432, Length=411, Percent_Identity=25.0608272506083, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356207, Length=488, Percent_Identity=23.3606557377049, Blast_Score=108, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_SPHAL (Q1GV83)

Other databases:

- EMBL:   CP000356
- RefSeq:   YP_615772.1
- ProteinModelPortal:   Q1GV83
- SMR:   Q1GV83
- GeneID:   4081807
- GenomeReviews:   CP000356_GR
- KEGG:   sal:Sala_0718
- NMPDR:   fig|317655.9.peg.666
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- ProtClustDB:   PRK14862
- BioCyc:   SALA317655:SALA_0718-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49699; Mature: 49568

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTKILPSQPKVGMVSLGCPKALVDSERILTKLRADGYGLSPDYAGADVVLVNTCGFLDS
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCH
AKEESLEAIGEAMAENGRVIVTGCMGKEADVIRARFPNVLAVTGAHQYEQVVEAVHDAAP
HHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCEEEECCCHHHHHHHHHHHHCCC
PTQGPFVDLVPEGGLKLTPRHYSYLKISEGCNHSCAFCIIPDLRGKLVSRRIDAVLREAE
CCCCCEEEEECCCCCEECCCCCEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
KLVAAGTKELLVISQDTSAYGVDIRHDPRQWHGREIRAHMTDLARELGQLRTSEGRAPWV
HHHHCCCCEEEEEECCCCEEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEE
RLHYVYPYPHVDAVIPLMAEGLLTPYLDIPFQHASPSVLKRMKRPANEAKVLERLKSWRA
EEEEECCCCCHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
IAPDIAIRSSFVVGFPGETEADFQYLLDWLEEAQLDRVGAFRFEPVAGAQANALPEPVPD
CCCCCEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCH
EVKEERYQRIMAKTAAISAAKLEAKIGRTLPVIIDEVGEADEDGSIGATGRSQADAPEID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GHVYLRDVAATLKAGDIVDVEIEDADEHDLFGVVT
CEEEEEHHHHHHCCCCEEEEEECCCCCCCEEEECC
>Mature Secondary Structure 
TTKILPSQPKVGMVSLGCPKALVDSERILTKLRADGYGLSPDYAGADVVLVNTCGFLDS
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCCCCH
AKEESLEAIGEAMAENGRVIVTGCMGKEADVIRARFPNVLAVTGAHQYEQVVEAVHDAAP
HHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHCCCCEEEECCCHHHHHHHHHHHHCCC
PTQGPFVDLVPEGGLKLTPRHYSYLKISEGCNHSCAFCIIPDLRGKLVSRRIDAVLREAE
CCCCCEEEEECCCCCEECCCCCEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHH
KLVAAGTKELLVISQDTSAYGVDIRHDPRQWHGREIRAHMTDLARELGQLRTSEGRAPWV
HHHHCCCCEEEEEECCCCEEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCEE
RLHYVYPYPHVDAVIPLMAEGLLTPYLDIPFQHASPSVLKRMKRPANEAKVLERLKSWRA
EEEEECCCCCHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHH
IAPDIAIRSSFVVGFPGETEADFQYLLDWLEEAQLDRVGAFRFEPVAGAQANALPEPVPD
CCCCCEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCH
EVKEERYQRIMAKTAAISAAKLEAKIGRTLPVIIDEVGEADEDGSIGATGRSQADAPEID
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
GHVYLRDVAATLKAGDIVDVEIEDADEHDLFGVVT
CEEEEEHHHHHHCCCCEEEEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA