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The map label for this gene is pmbA [H]

Identifier: 103486062

GI number: 103486062

Start: 581603

End: 582946

Strand: Reverse

Name: pmbA [H]

Synonym: Sala_0569

Alternate gene names: 103486062

Gene position: 582946-581603 (Counterclockwise)

Preceding gene: 103486063

Following gene: 103486061

Centisome position: 17.43

GC content: 70.54

Gene sequence:

>1344_bases
ATGCTCTCCGTTTCCCAAGCCCTCGACCGCGCGCAGATGCTGTGCGACGCCGCAACCAAGGCGGGCGCCGACGCCGCCGA
CGCGCTTTATTATTGCAACGCCGCAACATCGGTGACGATGCGCCTGGGCGCGCTCGAAGATGTCGAGCGATCCGAGGGGC
AGGATATTTCGCTGCGCGTCTTTGTCGGGCAGCGCAGCGCCAGCGTCTCGACCGCCGACATGGATGCGGGCGAGCTGGCC
AGGCTCGTCGAACGCTGCGTTGCAATGGCGCGCGAAGCACCCGAAGACCCCTATGCAGGCCTCGCGCCCGCCGACCTGCT
GTTCAGGGGCGCGGTGCCCGACCTCGACCTCGACGACGGCAGCGAGGCCGATCCTGCGGCGCTGCGCGAAGCCGCGCTGG
CGGTCGAAGACGCCGCGCGTGCGGTCGCGGGCGTGACCAACAGCGAAGGCGGCAGCGCGAGCCACAGCCGCACGCGCTTC
GCCCTCGCGACGAGCCACGGCTTTGCGGGCGGCTATGGATCGAGCGGCCACAGCCTGTCGGCGAGCGTGATCGCGGGCGA
AGGCGCCGCGATGCAGCGCGACTATGCCTGGCACAGCGCGCACCACCTGACCGACCTCGAAAGCGCCGCCGACATCGGCG
CGCGCGCGGGGGCCCGCGCCGTCGCGCGGCTGGAACCGGGCAAGGCGCCGAACGGCAAGCTGCCGGTCTTTTTCGACCCG
CGCGTCAGCGGCGGGATCGTCGGCCATCTGCTCGCCGCGATCGCCGGCCCCGCAATCGCCCGCGGGACGAGCTTCCTGAT
CGGCAAGGAAAAGGACATGCTGTTCGACAGCAGCATTATCATCCGCGACGAACCGCGCCGGCCACGCGGCCTGCGCAGCC
GCCCCTTCGACGGCGAAGGCCTGCCGACCGCGGCGCGCGACATCGTGGCGGACGGCAGGATCACCGGCTGGCTGCTCGAC
AGCGCATCGGCACGCCAGCTCGGCCTGACGCCGACGGGCCATGCGAGCCGCGGCGGTGGCGCGTCGGGGGTCGCGGCGAG
CAATCTTCATCTGGCGGCCGGGGCGCAAAGCCCCGCCGAACTGATGGCGGGCGTCGCCGAGGGCATCTATGTCACCGAAC
TGATCGGGCATGGCGTCAATCCGGTGACGGGTGACTATAGCCGCGGTGCGTCGGGCTTCATGATCCGCGGCGGCGCGATT
GCCGAACCGATCGCCGAGTTCACCGTCGCGGGCAATCTGGTCGACATGTTTGCGGCGCTGATCCCCGCCGACGACCTTAT
ATTCCGCCATGCCACCAACGCCCCGACGCTGCGCATCGACGGCATGACCGTCGCGTCGAGCTGA

Upstream 100 bases:

>100_bases
TCAGCGGCGCGATGGCCGCGACCGAGGCGATCGCCGCCATCGCGGGCTGAGTTTCCCGCCATCTTTTGATTTTTGTTCGC
GCGCCGTATAGGGCGCGGCG

Downstream 100 bases:

>100_bases
GACCGTCGTGCCCGGCCGCAACCTCGAAGCGGTGATCGCCGCGACGCGCGAGGTCGGCGACATGGCGATGGCGCGCTGGC
GCGGCGAAGGCAAGGCGGTC

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MLSVSQALDRAQMLCDAATKAGADAADALYYCNAATSVTMRLGALEDVERSEGQDISLRVFVGQRSASVSTADMDAGELA
RLVERCVAMAREAPEDPYAGLAPADLLFRGAVPDLDLDDGSEADPAALREAALAVEDAARAVAGVTNSEGGSASHSRTRF
ALATSHGFAGGYGSSGHSLSASVIAGEGAAMQRDYAWHSAHHLTDLESAADIGARAGARAVARLEPGKAPNGKLPVFFDP
RVSGGIVGHLLAAIAGPAIARGTSFLIGKEKDMLFDSSIIIRDEPRRPRGLRSRPFDGEGLPTAARDIVADGRITGWLLD
SASARQLGLTPTGHASRGGGASGVAASNLHLAAGAQSPAELMAGVAEGIYVTELIGHGVNPVTGDYSRGASGFMIRGGAI
AEPIAEFTVAGNLVDMFAALIPADDLIFRHATNAPTLRIDGMTVASS

Sequences:

>Translated_447_residues
MLSVSQALDRAQMLCDAATKAGADAADALYYCNAATSVTMRLGALEDVERSEGQDISLRVFVGQRSASVSTADMDAGELA
RLVERCVAMAREAPEDPYAGLAPADLLFRGAVPDLDLDDGSEADPAALREAALAVEDAARAVAGVTNSEGGSASHSRTRF
ALATSHGFAGGYGSSGHSLSASVIAGEGAAMQRDYAWHSAHHLTDLESAADIGARAGARAVARLEPGKAPNGKLPVFFDP
RVSGGIVGHLLAAIAGPAIARGTSFLIGKEKDMLFDSSIIIRDEPRRPRGLRSRPFDGEGLPTAARDIVADGRITGWLLD
SASARQLGLTPTGHASRGGGASGVAASNLHLAAGAQSPAELMAGVAEGIYVTELIGHGVNPVTGDYSRGASGFMIRGGAI
AEPIAEFTVAGNLVDMFAALIPADDLIFRHATNAPTLRIDGMTVASS
>Mature_447_residues
MLSVSQALDRAQMLCDAATKAGADAADALYYCNAATSVTMRLGALEDVERSEGQDISLRVFVGQRSASVSTADMDAGELA
RLVERCVAMAREAPEDPYAGLAPADLLFRGAVPDLDLDDGSEADPAALREAALAVEDAARAVAGVTNSEGGSASHSRTRF
ALATSHGFAGGYGSSGHSLSASVIAGEGAAMQRDYAWHSAHHLTDLESAADIGARAGARAVARLEPGKAPNGKLPVFFDP
RVSGGIVGHLLAAIAGPAIARGTSFLIGKEKDMLFDSSIIIRDEPRRPRGLRSRPFDGEGLPTAARDIVADGRITGWLLD
SASARQLGLTPTGHASRGGGASGVAASNLHLAAGAQSPAELMAGVAEGIYVTELIGHGVNPVTGDYSRGASGFMIRGGAI
AEPIAEFTVAGNLVDMFAALIPADDLIFRHATNAPTLRIDGMTVASS

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=453, Percent_Identity=36.4238410596026, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1789640, Length=263, Percent_Identity=25.0950570342205, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 45825; Mature: 45825

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSVSQALDRAQMLCDAATKAGADAADALYYCNAATSVTMRLGALEDVERSEGQDISLRV
CCCHHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCEEHHHHCCHHHHHHCCCCCEEEEE
FVGQRSASVSTADMDAGELARLVERCVAMAREAPEDPYAGLAPADLLFRGAVPDLDLDDG
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
SEADPAALREAALAVEDAARAVAGVTNSEGGSASHSRTRFALATSHGFAGGYGSSGHSLS
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEE
ASVIAGEGAAMQRDYAWHSAHHLTDLESAADIGARAGARAVARLEPGKAPNGKLPVFFDP
EEEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECC
RVSGGIVGHLLAAIAGPAIARGTSFLIGKEKDMLFDSSIIIRDEPRRPRGLRSRPFDGEG
CCCCHHHHHHHHHHHCCHHHCCCEEEEECCCCCEECCEEEEECCCCCCCCCCCCCCCCCC
LPTAARDIVADGRITGWLLDSASARQLGLTPTGHASRGGGASGVAASNLHLAAGAQSPAE
CCHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCCCCCCCCCCEECCEEEECCCCCHHH
LMAGVAEGIYVTELIGHGVNPVTGDYSRGASGFMIRGGAIAEPIAEFTVAGNLVDMFAAL
HHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
IPADDLIFRHATNAPTLRIDGMTVASS
CCCHHHHEEECCCCCEEEECCEEECCC
>Mature Secondary Structure
MLSVSQALDRAQMLCDAATKAGADAADALYYCNAATSVTMRLGALEDVERSEGQDISLRV
CCCHHHHHHHHHHHHHHHHHCCCCHHHHEEEECCCCEEHHHHCCHHHHHHCCCCCEEEEE
FVGQRSASVSTADMDAGELARLVERCVAMAREAPEDPYAGLAPADLLFRGAVPDLDLDDG
EEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
SEADPAALREAALAVEDAARAVAGVTNSEGGSASHSRTRFALATSHGFAGGYGSSGHSLS
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEE
ASVIAGEGAAMQRDYAWHSAHHLTDLESAADIGARAGARAVARLEPGKAPNGKLPVFFDP
EEEEECCCCHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEECC
RVSGGIVGHLLAAIAGPAIARGTSFLIGKEKDMLFDSSIIIRDEPRRPRGLRSRPFDGEG
CCCCHHHHHHHHHHHCCHHHCCCEEEEECCCCCEECCEEEEECCCCCCCCCCCCCCCCCC
LPTAARDIVADGRITGWLLDSASARQLGLTPTGHASRGGGASGVAASNLHLAAGAQSPAE
CCHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCCCCCCCCCCEECCEEEECCCCCHHH
LMAGVAEGIYVTELIGHGVNPVTGDYSRGASGFMIRGGAIAEPIAEFTVAGNLVDMFAAL
HHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
IPADDLIFRHATNAPTLRIDGMTVASS
CCCHHHHEEECCCCCEEEECCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]