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The map label for this gene is fumB [H]

Identifier: 103485869

GI number: 103485869

Start: 386090

End: 387613

Strand: Reverse

Name: fumB [H]

Synonym: Sala_0374

Alternate gene names: 103485869

Gene position: 387613-386090 (Counterclockwise)

Preceding gene: 103485875

Following gene: 103485867

Centisome position: 11.59

GC content: 64.57

Gene sequence:

>1524_bases
ATGAACACCGTCATCATTCGCGAAGACGATCTGATCGAAACCATCGCCGACGCGCTTCAGTACATCAGCTATTTTCATCC
GATGGACTATATTCGCGCGCTTGCCGCCGCCTATGAGCGCGAAGTGTCGCCCGCCGCCAAGGACGCGATGGCGCAGATAT
TGTCGAACAGCCGCATGTGCGCCGAGGGGCACCGACCGATCTGTCAGGACACCGGCATCGTCACCGTCTTCATCAAATGG
GGCATGGACTGCCGCCTCGATTCGCCCCGCAGCCTGCAACAGGTCGTCGACGAAGGCGTGCGCCGCGCCTATCTCCATCC
CGAAAACAAGCTGCGCGCCTCGATCCTCGCCGACCCGGCGTTCAGCCGCGTCAATACGAAGGACAATACGCCGTCGGTGC
TCAATGTCGAGATGGTCCCCGGCAACAAGGTCGTGATCGACGTCGCCGCAAAGGGCGGCGGCAGCGAAAACAAGTCGAAG
TTCAAGATGATGAACCCGTCGGATTCGATCGTCGACTGGGTGCTCGAAATGGTGCCGCAGATGGGCGCCGGCTGGTGCCC
GCCGGGGATGCTCGGCATCGGTATCGGCGGAACCGCCGAAAAGGCGATGCTGCTCGCCAAACAGTCGCTGATGGACCCGA
TCGACATGACCGAGCTGCTCGCGCGCGGCCCGAGCAATCCGACCGAGGAACTGCGGATCGAACTGTATGAAAAGGTCAAT
GCGCTCGGCATCGGCGCGCAGGGGCTGGGCGGGCTTTCGACCGTGCTCGACGTCAAGATCGCCGACTGGCCGACCCATGC
CGCCTCGAAGCCGGTCGCGATGATTCCGAACTGCGCCGCGACCCGCCACGCGCACATCACCCTCGACGGCAGCGGCCCGG
CCTATCTCGAACCGCCGAAACTCGCCGATTACCCGCAGATCGACTGGAAGCCCGACACCACGGCGAAGCGCGTCGATCTC
GACCATCTCACCCCCGAGATCGTCGCGAGCTGGAAACAGGGCGACCGCCTGCTCTTGAGCGGCAAGATGCTCACCGGCCG
CGACGCCGCGCACAAGCGCATCGCCGACATGCTCGCGAAGGGCGAGCAATTGCCCGTCGAGTTCAGGGGCCGCGTCATCT
ATTATGTCGGCCCGGTCGACCCCGTCGGCGAGGAAATCGTCGGCCCCGCCGGCCCGACGACCGCGACGCGCATGGACAAG
TTCATGGACATGATGCTGGGTCAGGGCCTGCTCGCCTGCGTCGGCAAGGCCGAACGCGGCCCCGCCGCGACGCAATCGAT
CGCGAAGCACAAGAGCGCGTACCTTATGGCGGTCGGCGGTGCCGCCTACCTTGTCGCGCGCGCGATCAAGGGCAGCAAGG
TGGTGGGCTTCGCCGACCTCGGCATGGAGGCGATCTACGAGTTCGAGGTGCAGGACTTCCCCGTCACCGTCGCGGTCGAC
AGCGAAGGCCAGAACGTCCACGTCAACGCGCCGAAGCTGTGGCAAAAGCGGATCAAGGATGAGGGGTTGCTGGCGGGGGT
GTAA

Upstream 100 bases:

>100_bases
CCTGATTTTGGGTTTTCCCTGTCGCGGCAAATGGCTTCCCACCCCGCCCCGCCGGGGCTAGGGAAGCGCCGAATCCCATT
AAAAGGGCGAAAGGCTGCAC

Downstream 100 bases:

>100_bases
GGCCTTCGCGCTCGGCGCGCATGCGTTCCATGAATAGCGCGACGTCCATCGCGTCATCGCCGGGCTCGCGCGGGGCGTAT
TTGGCAAAGGCGGCATAGGC

Product: tartrate/fumarate subfamily Fe-S type hydro-lyase alpha subunit

Products: NA

Alternate protein names: Fumarase [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MNTVIIREDDLIETIADALQYISYFHPMDYIRALAAAYEREVSPAAKDAMAQILSNSRMCAEGHRPICQDTGIVTVFIKW
GMDCRLDSPRSLQQVVDEGVRRAYLHPENKLRASILADPAFSRVNTKDNTPSVLNVEMVPGNKVVIDVAAKGGGSENKSK
FKMMNPSDSIVDWVLEMVPQMGAGWCPPGMLGIGIGGTAEKAMLLAKQSLMDPIDMTELLARGPSNPTEELRIELYEKVN
ALGIGAQGLGGLSTVLDVKIADWPTHAASKPVAMIPNCAATRHAHITLDGSGPAYLEPPKLADYPQIDWKPDTTAKRVDL
DHLTPEIVASWKQGDRLLLSGKMLTGRDAAHKRIADMLAKGEQLPVEFRGRVIYYVGPVDPVGEEIVGPAGPTTATRMDK
FMDMMLGQGLLACVGKAERGPAATQSIAKHKSAYLMAVGGAAYLVARAIKGSKVVGFADLGMEAIYEFEVQDFPVTVAVD
SEGQNVHVNAPKLWQKRIKDEGLLAGV

Sequences:

>Translated_507_residues
MNTVIIREDDLIETIADALQYISYFHPMDYIRALAAAYEREVSPAAKDAMAQILSNSRMCAEGHRPICQDTGIVTVFIKW
GMDCRLDSPRSLQQVVDEGVRRAYLHPENKLRASILADPAFSRVNTKDNTPSVLNVEMVPGNKVVIDVAAKGGGSENKSK
FKMMNPSDSIVDWVLEMVPQMGAGWCPPGMLGIGIGGTAEKAMLLAKQSLMDPIDMTELLARGPSNPTEELRIELYEKVN
ALGIGAQGLGGLSTVLDVKIADWPTHAASKPVAMIPNCAATRHAHITLDGSGPAYLEPPKLADYPQIDWKPDTTAKRVDL
DHLTPEIVASWKQGDRLLLSGKMLTGRDAAHKRIADMLAKGEQLPVEFRGRVIYYVGPVDPVGEEIVGPAGPTTATRMDK
FMDMMLGQGLLACVGKAERGPAATQSIAKHKSAYLMAVGGAAYLVARAIKGSKVVGFADLGMEAIYEFEVQDFPVTVAVD
SEGQNVHVNAPKLWQKRIKDEGLLAGV
>Mature_507_residues
MNTVIIREDDLIETIADALQYISYFHPMDYIRALAAAYEREVSPAAKDAMAQILSNSRMCAEGHRPICQDTGIVTVFIKW
GMDCRLDSPRSLQQVVDEGVRRAYLHPENKLRASILADPAFSRVNTKDNTPSVLNVEMVPGNKVVIDVAAKGGGSENKSK
FKMMNPSDSIVDWVLEMVPQMGAGWCPPGMLGIGIGGTAEKAMLLAKQSLMDPIDMTELLARGPSNPTEELRIELYEKVN
ALGIGAQGLGGLSTVLDVKIADWPTHAASKPVAMIPNCAATRHAHITLDGSGPAYLEPPKLADYPQIDWKPDTTAKRVDL
DHLTPEIVASWKQGDRLLLSGKMLTGRDAAHKRIADMLAKGEQLPVEFRGRVIYYVGPVDPVGEEIVGPAGPTTATRMDK
FMDMMLGQGLLACVGKAERGPAATQSIAKHKSAYLMAVGGAAYLVARAIKGSKVVGFADLGMEAIYEFEVQDFPVTVAVD
SEGQNVHVNAPKLWQKRIKDEGLLAGV

Specific function: It functions in the generation of fumarate for use as an anaerobic electron acceptor [H]

COG id: COG1951

COG function: function code C; Tartrate dehydratase alpha subunit/Fumarate hydratase class I, N-terminal domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I fumarase family [H]

Homologues:

Organism=Escherichia coli, GI1790564, Length=479, Percent_Identity=32.1503131524008, Blast_Score=204, Evalue=1e-53,
Organism=Escherichia coli, GI1787897, Length=491, Percent_Identity=31.9755600814664, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1789442, Length=294, Percent_Identity=30.2721088435374, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI1789443, Length=170, Percent_Identity=31.1764705882353, Blast_Score=81, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004646
- InterPro:   IPR004647
- InterPro:   IPR011167
- InterPro:   IPR020557 [H]

Pfam domain/function: PF05681 Fumerase; PF05683 Fumerase_C [H]

EC number: =4.2.1.2 [H]

Molecular weight: Translated: 54854; Mature: 54854

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTVIIREDDLIETIADALQYISYFHPMDYIRALAAAYEREVSPAAKDAMAQILSNSRMC
CCEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
AEGHRPICQDTGIVTVFIKWGMDCRLDSPRSLQQVVDEGVRRAYLHPENKLRASILADPA
CCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCH
FSRVNTKDNTPSVLNVEMVPGNKVVIDVAAKGGGSENKSKFKMMNPSDSIVDWVLEMVPQ
HHHCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHH
MGAGWCPPGMLGIGIGGTAEKAMLLAKQSLMDPIDMTELLARGPSNPTEELRIELYEKVN
HCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHH
ALGIGAQGLGGLSTVLDVKIADWPTHAASKPVAMIPNCAATRHAHITLDGSGPAYLEPPK
HHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEECCCCCCCCEEEEEECCCCCCCCCCCC
LADYPQIDWKPDTTAKRVDLDHLTPEIVASWKQGDRLLLSGKMLTGRDAAHKRIADMLAK
CCCCCCCCCCCCCCHHCCCHHHCCHHHHHHHCCCCEEEEECCEECCCHHHHHHHHHHHHC
GEQLPVEFRGRVIYYVGPVDPVGEEIVGPAGPTTATRMDKFMDMMLGQGLLACVGKAERG
CCCCCEEECCEEEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCC
PAATQSIAKHKSAYLMAVGGAAYLVARAIKGSKVVGFADLGMEAIYEFEVQDFPVTVAVD
CHHHHHHHHHCCEEEEEECHHHHHHHHHHCCCEEEEEHHHCHHHHEEEEECCCEEEEEEC
SEGQNVHVNAPKLWQKRIKDEGLLAGV
CCCCEEEECCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNTVIIREDDLIETIADALQYISYFHPMDYIRALAAAYEREVSPAAKDAMAQILSNSRMC
CCEEEECCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCC
AEGHRPICQDTGIVTVFIKWGMDCRLDSPRSLQQVVDEGVRRAYLHPENKLRASILADPA
CCCCCCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCH
FSRVNTKDNTPSVLNVEMVPGNKVVIDVAAKGGGSENKSKFKMMNPSDSIVDWVLEMVPQ
HHHCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCCCCEEEEECCCHHHHHHHHHHHHH
MGAGWCPPGMLGIGIGGTAEKAMLLAKQSLMDPIDMTELLARGPSNPTEELRIELYEKVN
HCCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHH
ALGIGAQGLGGLSTVLDVKIADWPTHAASKPVAMIPNCAATRHAHITLDGSGPAYLEPPK
HHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCEECCCCCCCCEEEEEECCCCCCCCCCCC
LADYPQIDWKPDTTAKRVDLDHLTPEIVASWKQGDRLLLSGKMLTGRDAAHKRIADMLAK
CCCCCCCCCCCCCCHHCCCHHHCCHHHHHHHCCCCEEEEECCEECCCHHHHHHHHHHHHC
GEQLPVEFRGRVIYYVGPVDPVGEEIVGPAGPTTATRMDKFMDMMLGQGLLACVGKAERG
CCCCCEEECCEEEEEECCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHCCCCCC
PAATQSIAKHKSAYLMAVGGAAYLVARAIKGSKVVGFADLGMEAIYEFEVQDFPVTVAVD
CHHHHHHHHHCCEEEEEECHHHHHHHHHHCCCEEEEEHHHCHHHHEEEEECCCEEEEEEC
SEGQNVHVNAPKLWQKRIKDEGLLAGV
CCCCEEEECCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2656658; 7610040; 9278503 [H]