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The map label for this gene is ycdT [H]

Identifier: 103485521

GI number: 103485521

Start: 17078

End: 18832

Strand: Direct

Name: ycdT [H]

Synonym: Sala_0023

Alternate gene names: 103485521

Gene position: 17078-18832 (Clockwise)

Preceding gene: 103485520

Following gene: 103485524

Centisome position: 0.51

GC content: 69.8

Gene sequence:

>1755_bases
ATGACTGGGGGATGCTCTCCTCTCGCGACGCGCTGCGGTCGGCTCTGGGGCCTGCTTGGCCTCATGCTGCTCACCATGCC
CGCGACACCCGCGCAGGCCCGCGCGCTCGACGTCGAGCGCGACCTGTGCCACGCGGTGAGCGGCACCGCGCTCGGCGACG
ATGCGCTGCCGCGCCTGCGCTTTGCCTGCCGCGGCACCCCCGATGATTATCAGAAGCAAAGCCTGTGGCTCCGCACCACG
CTCGACGAGCGGCCGGTGCAGGGCGGCCTGGCGCTGATGCTGCACAATTCGCGCTTCGACCGGCTGGCGGTCGGCTTTTC
CTATGCCGACGGCACAACACGCTGGCAGCGGGTGCGCAGCGGCGATTTCGGATCGCACTGGCGCGCCGGCGGGCAGATCC
TGTTCGATGCACCCCCGCGCGCTGTGCCGGTAACGGCCGTGACGATGCGTTTCGATGGCCTTGCCGCGCACCAACTTGTC
CGCGCGCGCATCCTCCCGGCGGATGAGGCAGGGGTGCAGGCCTCGGGCATGGCCGCGGCGATCGGCGCTGCGCTGACGCT
GCTGCTCGTCAGCGGCATTTACAGCCTGTCGCTCGCGGTTGCGGTGCGCCGCCAATATCTCGCCTGGCAAGCGGGGTGGT
CGGCGACGATGCTGCTATGGGGCACGCTCTGGTCGCAGGCGCACCTGGCGCTCCTGCCGACGATGGCCGGGACGGCGACG
GCGCAAATCTGCACCTTTCTTGCCTGTCTTGCCATTGCGGTGGCGACGGCGAGCGCCGTCACCGCGGTCGAGCGCGGCCG
GGGCGTGCGGGGCCTCAGGATCGTCGCATTGCTCCTTGGCGTCGGCGTCGGTCTGGCGGGCATCCCGCTCGCGCTGATCC
GCCGCGGCGACCTCAGCCTGCTCGCCGACCTGTTGGGCTTCGCCATCCTCGCCGACCTGATCGTCGTGGCGATCTATCTC
GCGCTGGCGTGGCGGCGCGGATCGGTCGAGGCGCGCGACTTTCTGGCGGCGTGGGGGCTGCCGATGGCGGTGCTGGCGTT
CATCCACATCGTCGACGTCAACAAGGACTTCTGGGGCGGCGGCTCGCAATTCGTCATGCTGGTCGCCGCGACCTGGCAGG
CGCTCTGGCTGGCCGCCGCCGCCACGCGCCGCCTCGCGCAGCTTCGGATCGAGCGCGACCATGCCCGCGATGCCGAGGCA
CGGGCGCAGACGCTGGCGCGCCGCGACCCGCTCACCGGCCTTGCGAACCGGCGCGGCTTCATCGACAGCGTCACCCCGCT
GCTCGACCGTGCACGCGTCGGCAATCTGCCCGCGGCGCTGCTGCTCGTCGATATCGACCGCTTCAAGTCGATCAACGACA
TCCATGGTCACGAGGCGGGCGACGCGGTGCTGTGTACGATCGGACAGCGGATCGAACGCTGGGAAGGGCCGATGTGTACC
GTCGCGCGGCTGGGCGGCGAGGAGTTCGGCCTGCTGACGATCGGCATGGAGGGTATTGTCCTCAGCCGTTTTGCCGAGAG
CATCCGGCGCGGCCTTGCCGACTGCGACCATAGTGTCATCGTCGGCGACCGACCGGTGACGGCCAGTATCGGCGTCGCCG
AAGTGCGTCCGGCGTGCGATTTCAAGCAGCTCTATCGCGTCGCCGACGAAGCACTTTACGACGCCAAGCGCGGCGGCCGC
AACAGGGTCATCGTCCAGCGGTACCGCGACGGACCGCCGCGCGCGCCAAGCCGCGAGATCGTGCTGTTGAAATAG

Upstream 100 bases:

>100_bases
TCGTGTCGCCGTGTCGCTCCGTGTCGGCCGCGACGGCAAATGTTGAAGAACGCCGCGCCATGGTAAAGATTTCCCAAGGA
TTTGGTGGGATAGCGCGGCG

Downstream 100 bases:

>100_bases
CGCGCCGCGATGCTGGCGATCAGAGCGAAGCCGCCTTGTTTTCGACCACCTCGAGCGGTGGCGGCGCCTTGGCCGCCATC
GCGCCATGGCGGCGGTGCGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MTGGCSPLATRCGRLWGLLGLMLLTMPATPAQARALDVERDLCHAVSGTALGDDALPRLRFACRGTPDDYQKQSLWLRTT
LDERPVQGGLALMLHNSRFDRLAVGFSYADGTTRWQRVRSGDFGSHWRAGGQILFDAPPRAVPVTAVTMRFDGLAAHQLV
RARILPADEAGVQASGMAAAIGAALTLLLVSGIYSLSLAVAVRRQYLAWQAGWSATMLLWGTLWSQAHLALLPTMAGTAT
AQICTFLACLAIAVATASAVTAVERGRGVRGLRIVALLLGVGVGLAGIPLALIRRGDLSLLADLLGFAILADLIVVAIYL
ALAWRRGSVEARDFLAAWGLPMAVLAFIHIVDVNKDFWGGGSQFVMLVAATWQALWLAAAATRRLAQLRIERDHARDAEA
RAQTLARRDPLTGLANRRGFIDSVTPLLDRARVGNLPAALLLVDIDRFKSINDIHGHEAGDAVLCTIGQRIERWEGPMCT
VARLGGEEFGLLTIGMEGIVLSRFAESIRRGLADCDHSVIVGDRPVTASIGVAEVRPACDFKQLYRVADEALYDAKRGGR
NRVIVQRYRDGPPRAPSREIVLLK

Sequences:

>Translated_584_residues
MTGGCSPLATRCGRLWGLLGLMLLTMPATPAQARALDVERDLCHAVSGTALGDDALPRLRFACRGTPDDYQKQSLWLRTT
LDERPVQGGLALMLHNSRFDRLAVGFSYADGTTRWQRVRSGDFGSHWRAGGQILFDAPPRAVPVTAVTMRFDGLAAHQLV
RARILPADEAGVQASGMAAAIGAALTLLLVSGIYSLSLAVAVRRQYLAWQAGWSATMLLWGTLWSQAHLALLPTMAGTAT
AQICTFLACLAIAVATASAVTAVERGRGVRGLRIVALLLGVGVGLAGIPLALIRRGDLSLLADLLGFAILADLIVVAIYL
ALAWRRGSVEARDFLAAWGLPMAVLAFIHIVDVNKDFWGGGSQFVMLVAATWQALWLAAAATRRLAQLRIERDHARDAEA
RAQTLARRDPLTGLANRRGFIDSVTPLLDRARVGNLPAALLLVDIDRFKSINDIHGHEAGDAVLCTIGQRIERWEGPMCT
VARLGGEEFGLLTIGMEGIVLSRFAESIRRGLADCDHSVIVGDRPVTASIGVAEVRPACDFKQLYRVADEALYDAKRGGR
NRVIVQRYRDGPPRAPSREIVLLK
>Mature_583_residues
TGGCSPLATRCGRLWGLLGLMLLTMPATPAQARALDVERDLCHAVSGTALGDDALPRLRFACRGTPDDYQKQSLWLRTTL
DERPVQGGLALMLHNSRFDRLAVGFSYADGTTRWQRVRSGDFGSHWRAGGQILFDAPPRAVPVTAVTMRFDGLAAHQLVR
ARILPADEAGVQASGMAAAIGAALTLLLVSGIYSLSLAVAVRRQYLAWQAGWSATMLLWGTLWSQAHLALLPTMAGTATA
QICTFLACLAIAVATASAVTAVERGRGVRGLRIVALLLGVGVGLAGIPLALIRRGDLSLLADLLGFAILADLIVVAIYLA
LAWRRGSVEARDFLAAWGLPMAVLAFIHIVDVNKDFWGGGSQFVMLVAATWQALWLAAAATRRLAQLRIERDHARDAEAR
AQTLARRDPLTGLANRRGFIDSVTPLLDRARVGNLPAALLLVDIDRFKSINDIHGHEAGDAVLCTIGQRIERWEGPMCTV
ARLGGEEFGLLTIGMEGIVLSRFAESIRRGLADCDHSVIVGDRPVTASIGVAEVRPACDFKQLYRVADEALYDAKRGGRN
RVIVQRYRDGPPRAPSREIVLLK

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=191, Percent_Identity=35.6020942408377, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI87081881, Length=166, Percent_Identity=38.5542168674699, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI1787802, Length=169, Percent_Identity=35.5029585798817, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI87081977, Length=197, Percent_Identity=30.9644670050761, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1786584, Length=194, Percent_Identity=30.4123711340206, Blast_Score=87, Evalue=4e-18,
Organism=Escherichia coli, GI1788085, Length=169, Percent_Identity=35.5029585798817, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI145693134, Length=157, Percent_Identity=38.2165605095541, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI87082007, Length=160, Percent_Identity=35, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1787816, Length=165, Percent_Identity=34.5454545454545, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1788956, Length=160, Percent_Identity=31.25, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1788381, Length=163, Percent_Identity=36.1963190184049, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787541, Length=179, Percent_Identity=30.1675977653631, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 62972; Mature: 62841

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTGGCSPLATRCGRLWGLLGLMLLTMPATPAQARALDVERDLCHAVSGTALGDDALPRLR
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
FACRGTPDDYQKQSLWLRTTLDERPVQGGLALMLHNSRFDRLAVGFSYADGTTRWQRVRS
HHCCCCCCHHHHHHEEEEECCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHC
GDFGSHWRAGGQILFDAPPRAVPVTAVTMRFDGLAAHQLVRARILPADEAGVQASGMAAA
CCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHH
IGAALTLLLVSGIYSLSLAVAVRRQYLAWQAGWSATMLLWGTLWSQAHLALLPTMAGTAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCHHH
AQICTFLACLAIAVATASAVTAVERGRGVRGLRIVALLLGVGVGLAGIPLALIRRGDLSL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHH
LADLLGFAILADLIVVAIYLALAWRRGSVEARDFLAAWGLPMAVLAFIHIVDVNKDFWGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHEECCCCCCCC
GSQFVMLVAATWQALWLAAAATRRLAQLRIERDHARDAEARAQTLARRDPLTGLANRRGF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHCCCC
IDSVTPLLDRARVGNLPAALLLVDIDRFKSINDIHGHEAGDAVLCTIGQRIERWEGPMCT
HHHHHHHHHHHHCCCCCHHHEEEEHHHHCCCCHHCCCCCCCEEEEHHHHHHHHHCCCEEE
VARLGGEEFGLLTIGMEGIVLSRFAESIRRGLADCDHSVIVGDRPVTASIGVAEVRPACD
HHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEECCCHHHCCCCCC
FKQLYRVADEALYDAKRGGRNRVIVQRYRDGPPRAPSREIVLLK
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEC
>Mature Secondary Structure 
TGGCSPLATRCGRLWGLLGLMLLTMPATPAQARALDVERDLCHAVSGTALGDDALPRLR
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
FACRGTPDDYQKQSLWLRTTLDERPVQGGLALMLHNSRFDRLAVGFSYADGTTRWQRVRS
HHCCCCCCHHHHHHEEEEECCCCCCCCCCEEEEEECCCCCEEEEEEEECCCCHHHHHHHC
GDFGSHWRAGGQILFDAPPRAVPVTAVTMRFDGLAAHQLVRARILPADEAGVQASGMAAA
CCCCCCCCCCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCHHHH
IGAALTLLLVSGIYSLSLAVAVRRQYLAWQAGWSATMLLWGTLWSQAHLALLPTMAGTAT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCHHH
AQICTFLACLAIAVATASAVTAVERGRGVRGLRIVALLLGVGVGLAGIPLALIRRGDLSL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHH
LADLLGFAILADLIVVAIYLALAWRRGSVEARDFLAAWGLPMAVLAFIHIVDVNKDFWGG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHEECCCCCCCC
GSQFVMLVAATWQALWLAAAATRRLAQLRIERDHARDAEARAQTLARRDPLTGLANRRGF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHCCCC
IDSVTPLLDRARVGNLPAALLLVDIDRFKSINDIHGHEAGDAVLCTIGQRIERWEGPMCT
HHHHHHHHHHHHCCCCCHHHEEEEHHHHCCCCHHCCCCCCCEEEEHHHHHHHHHCCCEEE
VARLGGEEFGLLTIGMEGIVLSRFAESIRRGLADCDHSVIVGDRPVTASIGVAEVRPACD
HHHCCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCEECCCHHHCCCCCC
FKQLYRVADEALYDAKRGGRNRVIVQRYRDGPPRAPSREIVLLK
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]