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The map label for this gene is polA [H]

Identifier: 197294838

GI number: 197294838

Start: 850502

End: 852532

Strand: Reverse

Name: polA [H]

Synonym: PAa_0824

Alternate gene names: 197294838

Gene position: 852532-850502 (Counterclockwise)

Preceding gene: 197294839

Following gene: 197294837

Centisome position: 96.88

GC content: 27.42

Gene sequence:

>2031_bases
ATGCAACATTTAGTTTTGGTGGATGGCAATTCGCTTGTTTTTAGAGCATATCATGCAACCGCTAATAACGGAAAGAAACT
TTTGCAAAATAAAAAAGGTGTTTATACTAACGCCCTTTTATCTTTTATTAAAATGTTTGAAAAAATTTTAGAACAAACAA
AAAATTATATATTAGTAGCCTTTGATACGCATCATCCAACCAAAAGGCATCAAATGTATGACAATTATAAAAAAGGTAGA
CCAGAAACACCTCAAGAATTAATTGATCAAATACCTTTAATTAAAAAATATTTAACTTTAAAAGGGGTTAAGCATCATTT
TCAAGCCGAATTTGAAGCAGACGATATTATTGGTACTTTAGCCAAAATGGCCAGCAACAAAAACATAAAAGTTTCTATTT
ATTCAAGCGACCAAGATTTATTGCAATTAGTAGATACAAACACAAGCGTTTATTTAATTAAAAAAGGATTAAAACTGATT
AAACATTATAATCCCAAAACTTTATTAGAAGAATACGGTTTGAAAGAATATCAAATGATCGATTATAAAGCTTTAATGGG
TGATTCTTCCGATAATATTAAAGGTGTTGCAGGAATAGGTCCTAAAACAGCCAAAAATTTGTTGCAAACTTATGATAATT
TAGATAATTTACTTTCTCATTTAGATGCGATTAAACCAAGTTTAAAAGAAAAAATTAATAATTGTATGGAAGATTTAAAA
CTATCTAAAATTTTGACAACGATTGATACCAAAGTTCCATTGCCTTTCGAATTGGAACAAACAGAAATAAAACATATGTC
ATCTTTTGAACTACAAGATTTTTATCAAGAGATGGATTTTAAAAGGTTAAAAATAACCCCCAACACTTCTTTGAAACCAG
ATTTAACTCAAAATTTTATTTACCAAAATATTGACCAAAACCAAGCCTTAGAACATGTAATTCAAACAATTACCGAAGAG
CAAACATGGTCTTTGTATTTTGAATTTGACCTTCATTACAAAAGTATTTTAGGAATTGGTTTTTCTTGCGGCAGCAATCA
TAATTTTTTCCTTGATCCTGATATTGCTTTGGCGAATCAATTTTTTATTTCTTATCTCAAAAATCCTGCTTTTTCTAAAA
ATGTTTTTAATACTTATCAAACACAGTTGTTTTTAAAACATAAAGGAATAATAATTGAAGGAATTTGTTTTGATTTATTG
CCAGCAGCCTATCTTTTATTTCCTTTGGAAAGCGATAATTTAATTGATCTTTTAACGAAAACAGATCTTTTAGGAGATTA
TGAACTCAATCATGTTAATTTAGAAAAAAAGACTGCCATCAAAGCTTTTTTATTTCACCAAAGCAAAGAGCACGTTTTAA
AACTACTTCAAACAAATAACCAATTATTTTTGTTTCATGAAATAGAATTACCTTTAGCATCTGTTTTTGCTAAATTAGAA
AATTGCCAAACATCATTGCCGTATTATCTTTTTAATAACAAACCTTTTTTAAACGAATTCCCTAAAGTTACTTTAAAAAC
ATTAGCTTTTTTAGGTAATGTTAAAAAAATCAAAGCTTTTTTTGCTTCTAAAAACCAAGTAATGAAAGAACAACAAAAAA
TAACTTTTTTCGGCCTTGACACAACCAATTACCGTCAAGATTTTTCATTGCCCAACTATTTTGATGATATGATTAACGAT
ATCGCTCAAGAATTGAATATATCTCTTTCTAATGCTAATATAATTATTAATCAATATTTAACTGTTGATGGCGATATAAC
TTGTTTCCTTGATAAGTTATTGCAACAAATCAAAGAACATCAATATTTAGAAACTTTTTGGAAAAGAAAAATATTTTGTT
TTTCTTTGCCTTTTTTAGACAAAAAGAAACATCCTTTATCTCTTTTGTTTTTATTTTTGCAAGCAAATATTTTAGACCTT
CAAAAAATCGCTTTTTTACAATTGTTTCGTCATTTGAAACCAATTAATCAATTAAAGCAAAACATACCTTTAATTGCCAA
AGCTTGCAACCCCTTTTTAGAGATTTTTTAA

Upstream 100 bases:

>100_bases
TAATGAAGAAACAAATTCACATTCAAATAACAATTCAAAGAAAGTTTTTTCAACTTTCTTTTTTTCATAATCCCCCTCGA
ATAACAAAAAGGAGAAAAAG

Downstream 100 bases:

>100_bases
GGCAATTTTGATTTTGGAAGATTTTTAAGTGCTAAAAACAACTGCCAACGGTCTTTTATTTAAATTTTTTTCAAAAATAA
TTGTTGATAAGTTATCTTAA

Product: DNA polymerase I

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 676; Mature: 676

Protein sequence:

>676_residues
MQHLVLVDGNSLVFRAYHATANNGKKLLQNKKGVYTNALLSFIKMFEKILEQTKNYILVAFDTHHPTKRHQMYDNYKKGR
PETPQELIDQIPLIKKYLTLKGVKHHFQAEFEADDIIGTLAKMASNKNIKVSIYSSDQDLLQLVDTNTSVYLIKKGLKLI
KHYNPKTLLEEYGLKEYQMIDYKALMGDSSDNIKGVAGIGPKTAKNLLQTYDNLDNLLSHLDAIKPSLKEKINNCMEDLK
LSKILTTIDTKVPLPFELEQTEIKHMSSFELQDFYQEMDFKRLKITPNTSLKPDLTQNFIYQNIDQNQALEHVIQTITEE
QTWSLYFEFDLHYKSILGIGFSCGSNHNFFLDPDIALANQFFISYLKNPAFSKNVFNTYQTQLFLKHKGIIIEGICFDLL
PAAYLLFPLESDNLIDLLTKTDLLGDYELNHVNLEKKTAIKAFLFHQSKEHVLKLLQTNNQLFLFHEIELPLASVFAKLE
NCQTSLPYYLFNNKPFLNEFPKVTLKTLAFLGNVKKIKAFFASKNQVMKEQQKITFFGLDTTNYRQDFSLPNYFDDMIND
IAQELNISLSNANIIINQYLTVDGDITCFLDKLLQQIKEHQYLETFWKRKIFCFSLPFLDKKKHPLSLLFLFLQANILDL
QKIAFLQLFRHLKPINQLKQNIPLIAKACNPFLEIF

Sequences:

>Translated_676_residues
MQHLVLVDGNSLVFRAYHATANNGKKLLQNKKGVYTNALLSFIKMFEKILEQTKNYILVAFDTHHPTKRHQMYDNYKKGR
PETPQELIDQIPLIKKYLTLKGVKHHFQAEFEADDIIGTLAKMASNKNIKVSIYSSDQDLLQLVDTNTSVYLIKKGLKLI
KHYNPKTLLEEYGLKEYQMIDYKALMGDSSDNIKGVAGIGPKTAKNLLQTYDNLDNLLSHLDAIKPSLKEKINNCMEDLK
LSKILTTIDTKVPLPFELEQTEIKHMSSFELQDFYQEMDFKRLKITPNTSLKPDLTQNFIYQNIDQNQALEHVIQTITEE
QTWSLYFEFDLHYKSILGIGFSCGSNHNFFLDPDIALANQFFISYLKNPAFSKNVFNTYQTQLFLKHKGIIIEGICFDLL
PAAYLLFPLESDNLIDLLTKTDLLGDYELNHVNLEKKTAIKAFLFHQSKEHVLKLLQTNNQLFLFHEIELPLASVFAKLE
NCQTSLPYYLFNNKPFLNEFPKVTLKTLAFLGNVKKIKAFFASKNQVMKEQQKITFFGLDTTNYRQDFSLPNYFDDMIND
IAQELNISLSNANIIINQYLTVDGDITCFLDKLLQQIKEHQYLETFWKRKIFCFSLPFLDKKKHPLSLLFLFLQANILDL
QKIAFLQLFRHLKPINQLKQNIPLIAKACNPFLEIF
>Mature_676_residues
MQHLVLVDGNSLVFRAYHATANNGKKLLQNKKGVYTNALLSFIKMFEKILEQTKNYILVAFDTHHPTKRHQMYDNYKKGR
PETPQELIDQIPLIKKYLTLKGVKHHFQAEFEADDIIGTLAKMASNKNIKVSIYSSDQDLLQLVDTNTSVYLIKKGLKLI
KHYNPKTLLEEYGLKEYQMIDYKALMGDSSDNIKGVAGIGPKTAKNLLQTYDNLDNLLSHLDAIKPSLKEKINNCMEDLK
LSKILTTIDTKVPLPFELEQTEIKHMSSFELQDFYQEMDFKRLKITPNTSLKPDLTQNFIYQNIDQNQALEHVIQTITEE
QTWSLYFEFDLHYKSILGIGFSCGSNHNFFLDPDIALANQFFISYLKNPAFSKNVFNTYQTQLFLKHKGIIIEGICFDLL
PAAYLLFPLESDNLIDLLTKTDLLGDYELNHVNLEKKTAIKAFLFHQSKEHVLKLLQTNNQLFLFHEIELPLASVFAKLE
NCQTSLPYYLFNNKPFLNEFPKVTLKTLAFLGNVKKIKAFFASKNQVMKEQQKITFFGLDTTNYRQDFSLPNYFDDMIND
IAQELNISLSNANIIINQYLTVDGDITCFLDKLLQQIKEHQYLETFWKRKIFCFSLPFLDKKKHPLSLLFLFLQANILDL
QKIAFLQLFRHLKPINQLKQNIPLIAKACNPFLEIF

Specific function: In addition to polymerase activity, this DNA polymerase exhibits 3' to 5' and 5' to 3' exonuclease activity [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=557, Percent_Identity=27.2890484739677, Blast_Score=149, Evalue=6e-37,
Organism=Escherichia coli, GI226510970, Length=262, Percent_Identity=26.7175572519084, Blast_Score=103, Evalue=5e-23,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR018320
- InterPro:   IPR002298
- InterPro:   IPR008918
- InterPro:   IPR003583
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 78452; Mature: 78452

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHLVLVDGNSLVFRAYHATANNGKKLLQNKKGVYTNALLSFIKMFEKILEQTKNYILVA
CCEEEEECCCEEEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
FDTHHPTKRHQMYDNYKKGRPETPQELIDQIPLIKKYLTLKGVKHHFQAEFEADDIIGTL
EECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AKMASNKNIKVSIYSSDQDLLQLVDTNTSVYLIKKGLKLIKHYNPKTLLEEYGLKEYQMI
HHHHCCCCEEEEEECCCHHHHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHHCCCCCEEEE
DYKALMGDSSDNIKGVAGIGPKTAKNLLQTYDNLDNLLSHLDAIKPSLKEKINNCMEDLK
HHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LSKILTTIDTKVPLPFELEQTEIKHMSSFELQDFYQEMDFKRLKITPNTSLKPDLTQNFI
HHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCCCCCHHHHHH
YQNIDQNQALEHVIQTITEEQTWSLYFEFDLHYKSILGIGFSCGSNHNFFLDPDIALANQ
HHCCCHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHCCEECCCCCCEEECCCHHHHHH
FFISYLKNPAFSKNVFNTYQTQLFLKHKGIIIEGICFDLLPAAYLLFPLESDNLIDLLTK
HHHHHHCCCCCCHHHHHHHHHHEEEEECCEEEEHHHHHHHHHHHHEEECCCCCHHHHHHH
TDLLGDYELNHVNLEKKTAIKAFLFHQSKEHVLKLLQTNNQLFLFHEIELPLASVFAKLE
HHCCCCCCCCEECCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
NCQTSLPYYLFNNKPFLNEFPKVTLKTLAFLGNVKKIKAFFASKNQVMKEQQKITFFGLD
HHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEECC
TTNYRQDFSLPNYFDDMINDIAQELNISLSNANIIINQYLTVDGDITCFLDKLLQQIKEH
CCCCCCCCCCCHHHHHHHHHHHHHHCEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHHH
QYLETFWKRKIFCFSLPFLDKKKHPLSLLFLFLQANILDLQKIAFLQLFRHLKPINQLKQ
HHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHH
NIPLIAKACNPFLEIF
CCCHHHHHCCHHHHCC
>Mature Secondary Structure
MQHLVLVDGNSLVFRAYHATANNGKKLLQNKKGVYTNALLSFIKMFEKILEQTKNYILVA
CCEEEEECCCEEEEEEEECCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEE
FDTHHPTKRHQMYDNYKKGRPETPQELIDQIPLIKKYLTLKGVKHHFQAEFEADDIIGTL
EECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
AKMASNKNIKVSIYSSDQDLLQLVDTNTSVYLIKKGLKLIKHYNPKTLLEEYGLKEYQMI
HHHHCCCCEEEEEECCCHHHHHHHCCCCEEEEHHHHHHHHHHCCHHHHHHHCCCCCEEEE
DYKALMGDSSDNIKGVAGIGPKTAKNLLQTYDNLDNLLSHLDAIKPSLKEKINNCMEDLK
HHHHHCCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
LSKILTTIDTKVPLPFELEQTEIKHMSSFELQDFYQEMDFKRLKITPNTSLKPDLTQNFI
HHHHHHHHCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCEEEEECCCCCCCCCHHHHHH
YQNIDQNQALEHVIQTITEEQTWSLYFEFDLHYKSILGIGFSCGSNHNFFLDPDIALANQ
HHCCCHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHCCEECCCCCCEEECCCHHHHHH
FFISYLKNPAFSKNVFNTYQTQLFLKHKGIIIEGICFDLLPAAYLLFPLESDNLIDLLTK
HHHHHHCCCCCCHHHHHHHHHHEEEEECCEEEEHHHHHHHHHHHHEEECCCCCHHHHHHH
TDLLGDYELNHVNLEKKTAIKAFLFHQSKEHVLKLLQTNNQLFLFHEIELPLASVFAKLE
HHCCCCCCCCEECCHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHH
NCQTSLPYYLFNNKPFLNEFPKVTLKTLAFLGNVKKIKAFFASKNQVMKEQQKITFFGLD
HHHCCCCEEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEEECC
TTNYRQDFSLPNYFDDMINDIAQELNISLSNANIIINQYLTVDGDITCFLDKLLQQIKEH
CCCCCCCCCCCHHHHHHHHHHHHHHCEEECCCEEEEEEEEEECCCHHHHHHHHHHHHHHH
QYLETFWKRKIFCFSLPFLDKKKHPLSLLFLFLQANILDLQKIAFLQLFRHLKPINQLKQ
HHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCHHHHHHH
NIPLIAKACNPFLEIF
CCCHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377 [H]