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The map label for this gene is nadE [H]

Identifier: 197294783

GI number: 197294783

Start: 777905

End: 779671

Strand: Direct

Name: nadE [H]

Synonym: PAa_0763

Alternate gene names: 197294783

Gene position: 777905-779671 (Clockwise)

Preceding gene: 197294782

Following gene: 197294784

Centisome position: 88.4

GC content: 28.69

Gene sequence:

>1767_bases
ATGTATAAAAACGGTTTAATTAAAATTCAATTATCCACTCCTAATTTAAAAATAGGAAATCCACAAGCAAATGCTTTCAG
TATAAATAAAATTCTTCAAGAAAGCAAAGCTCTTTTTATTCTTTTTCCTGAACTTTGTTTAAGTGGTTATACTGCTGGTG
ACCTTTTTTTTGAAACTTTCTTTTTAAAAGAAAATCTAAAGGCACTTGATTGGCTTTTAAAAAATAATACTTTTGAAGGG
GTTTTTATTTTAGGAATGCCTTTAGCTTTGCATGAAGTTTTATTTAATGTTGCAGTTGTTATCCAAAAAGATAAAATTTT
GGGTATAATACCTAAAAAAACCATCCCTAATTATAAAGAATTTATGGAAAAAAGATGGTTTCAATCAGGTAAAACCGTAG
ACAATCAACAAATCACTATTTTAGGTCAAGAAGTTCCTTTTGGCGATATTTTATTTGTAAATTCCAAACATGATATTATT
TTTGGGGTTGAAGTTTGTCAAGATTTATGGACTGTTTTTTCCCCTAGTGATTTAATGGCTTTAAATGGCGCTCATTTAAT
TTTTAATCTTTCTTCATCAACAGAACATGTGGGCAAAATCAATCCTCGCAAAATCGCTATTTTAGACCATTCACGCAAAC
AAATAGGCGGTTATTTTTATGCTAGTAGCGGAATTACTGAAATCACAGAATCAAACTTATTTTCCAACCATAAAATGGCA
GCTTCATTAGGACAAATGATTGGAGAAAAAAACTTATTTAATCAAGATATTAATTTAATCGTTGATCTAGACCTTGATTT
TATTAAATATCAAAGAAGGATTGACACTACTTACGGCGATCAAAGAATTGGCAACACCTTTCCTTTTTTAAAAGCTTTTT
TTCAAACAGAAGAAAGCAGTGATTATCATTTTGAATCCCCTATTAGTCAAACTCCTTTTATTAACCAAGATAGTTTACAA
AATGACTTGGAATTAGCCAATGAAATCCAAAAATTAGCTTTAAAAACGAAATTACAAGATTTAGATACCAAAGTTATTTT
AGAAATAACCGAAAACATAAATGATTTTGTTACCTTCATGGTTCTTTTACAAACTTTTTCCCTTTTGCAAAAACCACTTG
CAGATTTAATTATTATTCTAAATCCCATCTCTTTTCAAGATTTAAGTCATTACCAACTTTTAAAAACTTTTATCCAAGAA
CAAGGGGTTGTTAACTTTCAAGAAAAAGGCTTTTCTAATCAAACCCAATTTTTATCTTTTTTATCTCACAATCAACTTTT
AAAAGAAACAGATTCCCAAACAGCCTTGCTTTTAGAAAATAATAATTTTTCTGAGCTCGCTTTAGGTCAAATGAGTCAAA
GAGGCTATTATGATTATCTTTATAATGTTAATTCAGGGCTACCTCATCTTTTAATCAAAGAATTAATTTTTTATCACTTA
AAAAGCCCTCTTTTAAATCATTTCCAACCTTTAAAATTAATTTATCAACAAAAAGCACAAGCTTTAATGAATACTCAAAT
TATTATTGAAGATTTTATTCTTTTTCACCATCTTAAAAATGGTCATGAGCCTAACAAAATCGCTTGGTTATTGCAAATTG
TTTTTAATTATGATTCATTGCAAAGCCAAACTCTTGTTTTTAACTATTTAAAACGTTTTTTTCAAAGTCAATTTAAAAGA
CAAAATATGTCTCCCGGTCCTAAAATTTTAGAAAATAGTCTTTGTCCTAGAGGCGAATCGCGTTTGCCAGTTGGTTTAAA
AAGGTAA

Upstream 100 bases:

>100_bases
TTAATTTAGTTTCAAGTAAATATATTTTATGTTATAATCAAAAATTAATCTTAATGTTTTGCAACAAAGATAAAACAAAT
ATCAAGAAAGTAGGCAACAA

Downstream 100 bases:

>100_bases
TTGGGCATTCTAATAATTAAAATTTTATTTGTATTTTCAAGTAAAAACAATTACTAACTGATTTAAAAAAAGTTTTTCTT
GAAACCAAGAAATTAGTTAT

Product: NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MYKNGLIKIQLSTPNLKIGNPQANAFSINKILQESKALFILFPELCLSGYTAGDLFFETFFLKENLKALDWLLKNNTFEG
VFILGMPLALHEVLFNVAVVIQKDKILGIIPKKTIPNYKEFMEKRWFQSGKTVDNQQITILGQEVPFGDILFVNSKHDII
FGVEVCQDLWTVFSPSDLMALNGAHLIFNLSSSTEHVGKINPRKIAILDHSRKQIGGYFYASSGITEITESNLFSNHKMA
ASLGQMIGEKNLFNQDINLIVDLDLDFIKYQRRIDTTYGDQRIGNTFPFLKAFFQTEESSDYHFESPISQTPFINQDSLQ
NDLELANEIQKLALKTKLQDLDTKVILEITENINDFVTFMVLLQTFSLLQKPLADLIIILNPISFQDLSHYQLLKTFIQE
QGVVNFQEKGFSNQTQFLSFLSHNQLLKETDSQTALLLENNNFSELALGQMSQRGYYDYLYNVNSGLPHLLIKELIFYHL
KSPLLNHFQPLKLIYQQKAQALMNTQIIIEDFILFHHLKNGHEPNKIAWLLQIVFNYDSLQSQTLVFNYLKRFFQSQFKR
QNMSPGPKILENSLCPRGESRLPVGLKR

Sequences:

>Translated_588_residues
MYKNGLIKIQLSTPNLKIGNPQANAFSINKILQESKALFILFPELCLSGYTAGDLFFETFFLKENLKALDWLLKNNTFEG
VFILGMPLALHEVLFNVAVVIQKDKILGIIPKKTIPNYKEFMEKRWFQSGKTVDNQQITILGQEVPFGDILFVNSKHDII
FGVEVCQDLWTVFSPSDLMALNGAHLIFNLSSSTEHVGKINPRKIAILDHSRKQIGGYFYASSGITEITESNLFSNHKMA
ASLGQMIGEKNLFNQDINLIVDLDLDFIKYQRRIDTTYGDQRIGNTFPFLKAFFQTEESSDYHFESPISQTPFINQDSLQ
NDLELANEIQKLALKTKLQDLDTKVILEITENINDFVTFMVLLQTFSLLQKPLADLIIILNPISFQDLSHYQLLKTFIQE
QGVVNFQEKGFSNQTQFLSFLSHNQLLKETDSQTALLLENNNFSELALGQMSQRGYYDYLYNVNSGLPHLLIKELIFYHL
KSPLLNHFQPLKLIYQQKAQALMNTQIIIEDFILFHHLKNGHEPNKIAWLLQIVFNYDSLQSQTLVFNYLKRFFQSQFKR
QNMSPGPKILENSLCPRGESRLPVGLKR
>Mature_588_residues
MYKNGLIKIQLSTPNLKIGNPQANAFSINKILQESKALFILFPELCLSGYTAGDLFFETFFLKENLKALDWLLKNNTFEG
VFILGMPLALHEVLFNVAVVIQKDKILGIIPKKTIPNYKEFMEKRWFQSGKTVDNQQITILGQEVPFGDILFVNSKHDII
FGVEVCQDLWTVFSPSDLMALNGAHLIFNLSSSTEHVGKINPRKIAILDHSRKQIGGYFYASSGITEITESNLFSNHKMA
ASLGQMIGEKNLFNQDINLIVDLDLDFIKYQRRIDTTYGDQRIGNTFPFLKAFFQTEESSDYHFESPISQTPFINQDSLQ
NDLELANEIQKLALKTKLQDLDTKVILEITENINDFVTFMVLLQTFSLLQKPLADLIIILNPISFQDLSHYQLLKTFIQE
QGVVNFQEKGFSNQTQFLSFLSHNQLLKETDSQTALLLENNNFSELALGQMSQRGYYDYLYNVNSGLPHLLIKELIFYHL
KSPLLNHFQPLKLIYQQKAQALMNTQIIIEDFILFHHLKNGHEPNKIAWLLQIVFNYDSLQSQTLVFNYLKRFFQSQFKR
QNMSPGPKILENSLCPRGESRLPVGLKR

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=294, Percent_Identity=26.530612244898, Blast_Score=90, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17538756, Length=213, Percent_Identity=29.5774647887324, Blast_Score=78, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6321865, Length=266, Percent_Identity=27.8195488721804, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24641841, Length=312, Percent_Identity=25.3205128205128, Blast_Score=79, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24641843, Length=312, Percent_Identity=25.3205128205128, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 67595; Mature: 67595

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKNGLIKIQLSTPNLKIGNPQANAFSINKILQESKALFILFPELCLSGYTAGDLFFETF
CCCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHCCEEEEEEHHHHHCCCCHHHHHHHHH
FLKENLKALDWLLKNNTFEGVFILGMPLALHEVLFNVAVVIQKDKILGIIPKKTIPNYKE
HHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHEEECCCEEEECCCCCCCCHHH
FMEKRWFQSGKTVDNQQITILGQEVPFGDILFVNSKHDIIFGVEVCQDLWTVFSPSDLMA
HHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCCEE
LNGAHLIFNLSSSTEHVGKINPRKIAILDHSRKQIGGYFYASSGITEITESNLFSNHKMA
ECCCEEEEEECCCCCHHCCCCCCEEEEEECCHHHHCCEEEECCCCHHHHHHHHHCCHHHH
ASLGQMIGEKNLFNQDINLIVDLDLDFIKYQRRIDTTYGDQRIGNTFPFLKAFFQTEESS
HHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCC
DYHFESPISQTPFINQDSLQNDLELANEIQKLALKTKLQDLDTKVILEITENINDFVTFM
CCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VLLQTFSLLQKPLADLIIILNPISFQDLSHYQLLKTFIQEQGVVNFQEKGFSNQTQFLSF
HHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHCCCEEHHHCCCCCHHHHHHH
LSHNQLLKETDSQTALLLENNNFSELALGQMSQRGYYDYLYNVNSGLPHLLIKELIFYHL
HHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHH
KSPLLNHFQPLKLIYQQKAQALMNTQIIIEDFILFHHLKNGHEPNKIAWLLQIVFNYDSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCH
QSQTLVFNYLKRFFQSQFKRQNMSPGPKILENSLCPRGESRLPVGLKR
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MYKNGLIKIQLSTPNLKIGNPQANAFSINKILQESKALFILFPELCLSGYTAGDLFFETF
CCCCCEEEEEECCCCEEECCCCCCHHHHHHHHHHCCEEEEEEHHHHHCCCCHHHHHHHHH
FLKENLKALDWLLKNNTFEGVFILGMPLALHEVLFNVAVVIQKDKILGIIPKKTIPNYKE
HHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHEEECCCEEEECCCCCCCCHHH
FMEKRWFQSGKTVDNQQITILGQEVPFGDILFVNSKHDIIFGVEVCQDLWTVFSPSDLMA
HHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEECCCCEEEHHHHHHHHHHHCCCCCCEE
LNGAHLIFNLSSSTEHVGKINPRKIAILDHSRKQIGGYFYASSGITEITESNLFSNHKMA
ECCCEEEEEECCCCCHHCCCCCCEEEEEECCHHHHCCEEEECCCCHHHHHHHHHCCHHHH
ASLGQMIGEKNLFNQDINLIVDLDLDFIKYQRRIDTTYGDQRIGNTFPFLKAFFQTEESS
HHHHHHHCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCC
DYHFESPISQTPFINQDSLQNDLELANEIQKLALKTKLQDLDTKVILEITENINDFVTFM
CCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
VLLQTFSLLQKPLADLIIILNPISFQDLSHYQLLKTFIQEQGVVNFQEKGFSNQTQFLSF
HHHHHHHHHHHHHHHHEEEECCCCCCCCHHHHHHHHHHHHCCCEEHHHCCCCCHHHHHHH
LSHNQLLKETDSQTALLLENNNFSELALGQMSQRGYYDYLYNVNSGLPHLLIKELIFYHL
HHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHH
KSPLLNHFQPLKLIYQQKAQALMNTQIIIEDFILFHHLKNGHEPNKIAWLLQIVFNYDSL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCH
QSQTLVFNYLKRFFQSQFKRQNMSPGPKILENSLCPRGESRLPVGLKR
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]