Click here to switch to the map view.

The map label for this gene is dppA [H]

Identifier: 197294776

GI number: 197294776

Start: 770273

End: 771865

Strand: Direct

Name: dppA [H]

Synonym: PAa_0756

Alternate gene names: 197294776

Gene position: 770273-771865 (Clockwise)

Preceding gene: 197294775

Following gene: 197294777

Centisome position: 87.54

GC content: 27.24

Gene sequence:

>1593_bases
ATGAATTTAGCTAAATTCAAGGCTTTTTGTTCTAAACACAGAAAAGCAATTATTTATACATCATTATTATTTTTGATTAT
TTTTGTTATCACTATAACTGCCATAATAGTGAAGAAAGGTAATAAAAAAGCTTTCGATCCAAATACAGTCAATATAACAA
TGTCAAGCGATGTCAAAGGGTTTGATGTTTGTAATACTTCACATACTTCGACTGCTTACGCAAATTGGACCTTTTCTTTA
ATGCATGATACTTTATTATATAAAAATAAAAAAGGTGAAGTAAAAAGTATGTTGGCAACCCTTGATGAAAAAGACAGTGA
GGGCAAACGATTTACTTTTACTTTAAAAGATAAAATCTTATTTCATAATGGAAAGCTTTTAACAACTGATGATATTGTAT
TTACTTTTGAAAGAGGAAAAGCAAACGAACATGCTCAATTCTTAGAAATTGAAGCTGTTGAAAAAATAGATGCTAAAAAA
TTTATAATTGTTGTCAAAGATGCTAGTCTTTGGAGTAACTTTAAATTTTACAGCTTTTTTAAAGTTTTAAGCAAACAAAC
TATACAAGAAGCGCAAAACCAAGGAGATGAAGCTATTCAAAAAGCTTTACAAGTGGGTACAGGTCCTTATAAATTAGTTG
ATTATAATAAAGATGATAGACTTAAATTTGAATTATTTGATTCTTATTATGATAAAGAACGAATTAAGACAAGCCCTCCA
AAAATTAATTTAAAAATTTCTAAAAACGACGACACTAATTTGCAAGAACTAGAAAACGGCCAAATTGATGCTATCCTTAA
TTATCCTAATTCCAAAATCAAAGACGTTAAACAAAATCTTTCCAATAAAATTAAAATAGTTGAAAACGATTCTGTGGGTT
GTTCGTATATTTATATTAATAAAAAAACGACTTCTTTGGAAGCAAGAAAAGCAATTAGTCAAACAATTGATACTCAAGCA
ATTAAAGATCAATTAGAGTTACCAGGGAAAAAATTAAATAGCTTTTTGCCTACTTCTTTAATAGGTAGCGACCTTGCTTT
AAAATATGATATAAATCTTTCAGAGGCTAAAAATATTGTTAATAATCTAAGTTCTAACCAAAAAAAATTAAAAATAGGGA
TTAGCATAGGGAACCCTTTGGATGTCGCTGGTAAAGTGGTAGAACAATTAAGAAATGTTGGTTTTGAAGTAAATTTAAAT
CAAGAAGATTTTAATTCTTTGACGGAAAATATGATAAAAGAAGATACTGATTATAACATGATTTTTCTTGGTGAAAATCA
TGAATTAGAATACGGCCACAAAGCTTTAACTGATTATTTTTTCTCCAATAATAATGAGAGTAATTTCTGTCATATCGATA
ATAACGATCAAACATCAATTGAAGATAAATTGAAATTAGCTCAAAAAGAATTAAATAAAGTGGAATATATTTCTTTAATT
CAACAAGTAAGCAAATATATCCATCAACAAGTTTATGTAATTCCTTTATATACAAGTCCTTCTTATTGTCTTACTTCCAA
ACACATTGAAAAAGGATTTGAAACAGATGCTTTTTCTAATTTTGAATTTACTACTATTATCAAAAAACAATAG

Upstream 100 bases:

>100_bases
CTAAAAAAAATTTTAATTGATAAAAAAATCTATAAATTTGGCAACAGTTCAAAAATTAATTATATTTAAAGATTTAATCC
AAGAAAGAAAGAAAATTAAA

Downstream 100 bases:

>100_bases
TTAATTTTATGTGTAAATGTTTGTTGGTTGTTAAGGTGGTTTTTAACTTTAACACCAACTTCTTTTGCAATTTTGGCATG
TTTCAAAGACATGCCAAAAA

Product: ABC-type peptide/nickel transport system substrate-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MNLAKFKAFCSKHRKAIIYTSLLFLIIFVITITAIIVKKGNKKAFDPNTVNITMSSDVKGFDVCNTSHTSTAYANWTFSL
MHDTLLYKNKKGEVKSMLATLDEKDSEGKRFTFTLKDKILFHNGKLLTTDDIVFTFERGKANEHAQFLEIEAVEKIDAKK
FIIVVKDASLWSNFKFYSFFKVLSKQTIQEAQNQGDEAIQKALQVGTGPYKLVDYNKDDRLKFELFDSYYDKERIKTSPP
KINLKISKNDDTNLQELENGQIDAILNYPNSKIKDVKQNLSNKIKIVENDSVGCSYIYINKKTTSLEARKAISQTIDTQA
IKDQLELPGKKLNSFLPTSLIGSDLALKYDINLSEAKNIVNNLSSNQKKLKIGISIGNPLDVAGKVVEQLRNVGFEVNLN
QEDFNSLTENMIKEDTDYNMIFLGENHELEYGHKALTDYFFSNNNESNFCHIDNNDQTSIEDKLKLAQKELNKVEYISLI
QQVSKYIHQQVYVIPLYTSPSYCLTSKHIEKGFETDAFSNFEFTTIIKKQ

Sequences:

>Translated_530_residues
MNLAKFKAFCSKHRKAIIYTSLLFLIIFVITITAIIVKKGNKKAFDPNTVNITMSSDVKGFDVCNTSHTSTAYANWTFSL
MHDTLLYKNKKGEVKSMLATLDEKDSEGKRFTFTLKDKILFHNGKLLTTDDIVFTFERGKANEHAQFLEIEAVEKIDAKK
FIIVVKDASLWSNFKFYSFFKVLSKQTIQEAQNQGDEAIQKALQVGTGPYKLVDYNKDDRLKFELFDSYYDKERIKTSPP
KINLKISKNDDTNLQELENGQIDAILNYPNSKIKDVKQNLSNKIKIVENDSVGCSYIYINKKTTSLEARKAISQTIDTQA
IKDQLELPGKKLNSFLPTSLIGSDLALKYDINLSEAKNIVNNLSSNQKKLKIGISIGNPLDVAGKVVEQLRNVGFEVNLN
QEDFNSLTENMIKEDTDYNMIFLGENHELEYGHKALTDYFFSNNNESNFCHIDNNDQTSIEDKLKLAQKELNKVEYISLI
QQVSKYIHQQVYVIPLYTSPSYCLTSKHIEKGFETDAFSNFEFTTIIKKQ
>Mature_530_residues
MNLAKFKAFCSKHRKAIIYTSLLFLIIFVITITAIIVKKGNKKAFDPNTVNITMSSDVKGFDVCNTSHTSTAYANWTFSL
MHDTLLYKNKKGEVKSMLATLDEKDSEGKRFTFTLKDKILFHNGKLLTTDDIVFTFERGKANEHAQFLEIEAVEKIDAKK
FIIVVKDASLWSNFKFYSFFKVLSKQTIQEAQNQGDEAIQKALQVGTGPYKLVDYNKDDRLKFELFDSYYDKERIKTSPP
KINLKISKNDDTNLQELENGQIDAILNYPNSKIKDVKQNLSNKIKIVENDSVGCSYIYINKKTTSLEARKAISQTIDTQA
IKDQLELPGKKLNSFLPTSLIGSDLALKYDINLSEAKNIVNNLSSNQKKLKIGISIGNPLDVAGKVVEQLRNVGFEVNLN
QEDFNSLTENMIKEDTDYNMIFLGENHELEYGHKALTDYFFSNNNESNFCHIDNNDQTSIEDKLKLAQKELNKVEYISLI
QQVSKYIHQQVYVIPLYTSPSYCLTSKHIEKGFETDAFSNFEFTTIIKKQ

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=507, Percent_Identity=20.9072978303748, Blast_Score=76, Evalue=6e-15,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 60551; Mature: 60551

Theoretical pI: Translated: 8.16; Mature: 8.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLAKFKAFCSKHRKAIIYTSLLFLIIFVITITAIIVKKGNKKAFDPNTVNITMSSDVKG
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCC
FDVCNTSHTSTAYANWTFSLMHDTLLYKNKKGEVKSMLATLDEKDSEGKRFTFTLKDKIL
CEEECCCCCCEEEECEEHHHHHHHHHEECCCCHHHHHHHHHCCCCCCCCEEEEEEEEEEE
FHNGKLLTTDDIVFTFERGKANEHAQFLEIEAVEKIDAKKFIIVVKDASLWSNFKFYSFF
EECCEEEEECCEEEEEECCCCCCCHHEEEEHHHHHCCCCEEEEEEECCHHHCCCHHHHHH
KVLSKQTIQEAQNQGDEAIQKALQVGTGPYKLVDYNKDDRLKFELFDSYYDKERIKTSPP
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCEEEEEHHHHHHHHHCCCCCC
KINLKISKNDDTNLQELENGQIDAILNYPNSKIKDVKQNLSNKIKIVENDSVGCSYIYIN
EEEEEEECCCCCCHHHHCCCCEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEE
KKTTSLEARKAISQTIDTQAIKDQLELPGKKLNSFLPTSLIGSDLALKYDINLSEAKNIV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHCCCCEEEEEECCHHHHHHHH
NNLSSNQKKLKIGISIGNPLDVAGKVVEQLRNVGFEVNLNQEDFNSLTENMIKEDTDYNM
HHCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHCCCCCCE
IFLGENHELEYGHKALTDYFFSNNNESNFCHIDNNDQTSIEDKLKLAQKELNKVEYISLI
EEEECCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
QQVSKYIHQQVYVIPLYTSPSYCLTSKHIEKGFETDAFSNFEFTTIIKKQ
HHHHHHHHCEEEEEEEECCCCHHEEHHHHHCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MNLAKFKAFCSKHRKAIIYTSLLFLIIFVITITAIIVKKGNKKAFDPNTVNITMSSDVKG
CCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCC
FDVCNTSHTSTAYANWTFSLMHDTLLYKNKKGEVKSMLATLDEKDSEGKRFTFTLKDKIL
CEEECCCCCCEEEECEEHHHHHHHHHEECCCCHHHHHHHHHCCCCCCCCEEEEEEEEEEE
FHNGKLLTTDDIVFTFERGKANEHAQFLEIEAVEKIDAKKFIIVVKDASLWSNFKFYSFF
EECCEEEEECCEEEEEECCCCCCCHHEEEEHHHHHCCCCEEEEEEECCHHHCCCHHHHHH
KVLSKQTIQEAQNQGDEAIQKALQVGTGPYKLVDYNKDDRLKFELFDSYYDKERIKTSPP
HHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCEEEEEHHHHHHHHHCCCCCC
KINLKISKNDDTNLQELENGQIDAILNYPNSKIKDVKQNLSNKIKIVENDSVGCSYIYIN
EEEEEEECCCCCCHHHHCCCCEEEEEECCCHHHHHHHHHHCCCEEEEECCCCCEEEEEEE
KKTTSLEARKAISQTIDTQAIKDQLELPGKKLNSFLPTSLIGSDLALKYDINLSEAKNIV
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHCCCCEEEEEECCHHHHHHHH
NNLSSNQKKLKIGISIGNPLDVAGKVVEQLRNVGFEVNLNQEDFNSLTENMIKEDTDYNM
HHCCCCCEEEEEEEEECCCHHHHHHHHHHHHHCCEEEEECHHHHHHHHHHHHHCCCCCCE
IFLGENHELEYGHKALTDYFFSNNNESNFCHIDNNDQTSIEDKLKLAQKELNKVEYISLI
EEEECCCCCCCCHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
QQVSKYIHQQVYVIPLYTSPSYCLTSKHIEKGFETDAFSNFEFTTIIKKQ
HHHHHHHHCEEEEEEEECCCCHHEEHHHHHCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]