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The map label for this gene is pcnB [H]

Identifier: 197294745

GI number: 197294745

Start: 729634

End: 730872

Strand: Reverse

Name: pcnB [H]

Synonym: PAa_0711

Alternate gene names: 197294745

Gene position: 730872-729634 (Counterclockwise)

Preceding gene: 197294746

Following gene: 197294744

Centisome position: 83.06

GC content: 25.5

Gene sequence:

>1239_bases
ATGAAAAATTTATCATCTCTTGGAAAAATTAAAAAAGCACAAAAAATTATTCAAATATTTAAACAAAAAGGATTTGAAGC
CTTTATTGTAGGTGGCGCTGTGAGAGATTATTTATTAAATATCCCATTCAACCAAGATGTTGATATTACAACAAATGCTG
TGCCACAAGATATTAATGCTATTTTTCAAAGCCAAAAACGAGCTTATTACGGATGCATTAAAATTATTTTTGAAGAAGAA
AGTTTTGAAATCACCACTTACAGAGAAGAAGATATTTATTTAAATCACCGTCATCCTAACAAAATCGTTTTTATTCAGGA
AGTTAAAAAAGATGTTATTAGAAGAGATTTTACTATTAATGCTTTATTAATGGATGAAAAGGGACAAATTTTTGATTGGA
CCGAAGGAAAATCAGATTTAACAAAAAAAATACTTCGCACGATTCAAAATCCTTTTATAAGTTTTGAAAAAGATGCCTTA
AGAATAATGAGAGCTTTTTATTTTCAAGCTAAATTAAATTTTCAAATAGAAAAACAAACGCAAATTGCTTTAACGAAACA
TTCACATTTATTAACCCAAATATCTTCTTCAAGAATTTATGAATATTTACAAAAAATAATTACTTATCCTCATTGGCAAG
CGAGTTTTAAAATGATGATTGAAACAAAGGCTCATTTATTTTTAAAACCCATTACAAAAGCCATTATTTTTTTTGCTTCT
TTGAAAGATGAAGAATTTACAAACAAAAACTTAGAAGAAAATATTTTTTGGAGCATTGCATTTGTTTTTAACAAAAATAT
TTTTTCCCTTTTCCCTATTAATTCTAAAAAAAAGAAACAATGCCAAACCTTAATCATTTTAAGTAATCATATTTTCACAA
ATTTAAAATTGAATTTATTCACACATCAACTTTCCAATTGTTTGTTAGCTTATAAAATCAATTACCTTTTATCTCCTCAA
AAATCAAAAATTTTTTTGATTGAAAATTTAGACAATATCAAACAAACATATAAAAATCTCCCTTTGAAAAACTTTCAAGA
ATTAAAAATGGATTGGCGCCAAATAGCCCCTCATCGAATCCCAGAAATAAAACAAAAAGTAATTCAAGCTGTTTTAGAAA
AAAAAATAACTAACGAATTAGAAGAATTAAAAGCATTTGTTTTCAATCACAAACATAATGAAAAAGTGGATGCTTGGCAT
AAAAAAGAAAAAAAGAGAAAAAAATATGACAAACAATAA

Upstream 100 bases:

>100_bases
TATTTTATTATAAAGACCATCTTTTAATGGTCTTTTTTTATTAATTAAAAACATTATTTGGATGGAGCACGATCAACCAA
TTTACAAAAGTAATAAAAAT

Downstream 100 bases:

>100_bases
TATTATTATAAAACAAAAAGATCAAATAAATCATTTAGTAGGAAAAGTAACGAGTATTAATCAAGGAAATCATTTTTATA
ATGTCAATTTGCAATTAACT

Product: Poly(A) polymerase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MKNLSSLGKIKKAQKIIQIFKQKGFEAFIVGGAVRDYLLNIPFNQDVDITTNAVPQDINAIFQSQKRAYYGCIKIIFEEE
SFEITTYREEDIYLNHRHPNKIVFIQEVKKDVIRRDFTINALLMDEKGQIFDWTEGKSDLTKKILRTIQNPFISFEKDAL
RIMRAFYFQAKLNFQIEKQTQIALTKHSHLLTQISSSRIYEYLQKIITYPHWQASFKMMIETKAHLFLKPITKAIIFFAS
LKDEEFTNKNLEENIFWSIAFVFNKNIFSLFPINSKKKKQCQTLIILSNHIFTNLKLNLFTHQLSNCLLAYKINYLLSPQ
KSKIFLIENLDNIKQTYKNLPLKNFQELKMDWRQIAPHRIPEIKQKVIQAVLEKKITNELEELKAFVFNHKHNEKVDAWH
KKEKKRKKYDKQ

Sequences:

>Translated_412_residues
MKNLSSLGKIKKAQKIIQIFKQKGFEAFIVGGAVRDYLLNIPFNQDVDITTNAVPQDINAIFQSQKRAYYGCIKIIFEEE
SFEITTYREEDIYLNHRHPNKIVFIQEVKKDVIRRDFTINALLMDEKGQIFDWTEGKSDLTKKILRTIQNPFISFEKDAL
RIMRAFYFQAKLNFQIEKQTQIALTKHSHLLTQISSSRIYEYLQKIITYPHWQASFKMMIETKAHLFLKPITKAIIFFAS
LKDEEFTNKNLEENIFWSIAFVFNKNIFSLFPINSKKKKQCQTLIILSNHIFTNLKLNLFTHQLSNCLLAYKINYLLSPQ
KSKIFLIENLDNIKQTYKNLPLKNFQELKMDWRQIAPHRIPEIKQKVIQAVLEKKITNELEELKAFVFNHKHNEKVDAWH
KKEKKRKKYDKQ
>Mature_412_residues
MKNLSSLGKIKKAQKIIQIFKQKGFEAFIVGGAVRDYLLNIPFNQDVDITTNAVPQDINAIFQSQKRAYYGCIKIIFEEE
SFEITTYREEDIYLNHRHPNKIVFIQEVKKDVIRRDFTINALLMDEKGQIFDWTEGKSDLTKKILRTIQNPFISFEKDAL
RIMRAFYFQAKLNFQIEKQTQIALTKHSHLLTQISSSRIYEYLQKIITYPHWQASFKMMIETKAHLFLKPITKAIIFFAS
LKDEEFTNKNLEENIFWSIAFVFNKNIFSLFPINSKKKKQCQTLIILSNHIFTNLKLNLFTHQLSNCLLAYKINYLLSPQ
KSKIFLIENLDNIKQTYKNLPLKNFQELKMDWRQIAPHRIPEIKQKVIQAVLEKKITNELEELKAFVFNHKHNEKVDAWH
KKEKKRKKYDKQ

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=208, Percent_Identity=33.1730769230769, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI87081691, Length=228, Percent_Identity=29.3859649122807, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789436, Length=219, Percent_Identity=27.8538812785388, Blast_Score=78, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71995920, Length=217, Percent_Identity=30.4147465437788, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI281360112, Length=203, Percent_Identity=31.5270935960591, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21357337, Length=203, Percent_Identity=31.5270935960591, Blast_Score=100, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 48981; Mature: 48981

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLSSLGKIKKAQKIIQIFKQKGFEAFIVGGAVRDYLLNIPFNQDVDITTNAVPQDINA
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHEECCCCCCCCCCCCCCCHHHHH
IFQSQKRAYYGCIKIIFEEESFEITTYREEDIYLNHRHPNKIVFIQEVKKDVIRRDFTIN
HHHHHHHHHEEEEEEEEECCCEEEEEEECCCEEEECCCCCEEEEHHHHHHHHHHCCCEEE
ALLMDEKGQIFDWTEGKSDLTKKILRTIQNPFISFEKDALRIMRAFYFQAKLNFQIEKQT
EEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEEECCC
QIALTKHSHLLTQISSSRIYEYLQKIITYPHWQASFKMMIETKAHLFLKPITKAIIFFAS
EEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEHHHHHHHHHHHHHHHHHHHH
LKDEEFTNKNLEENIFWSIAFVFNKNIFSLFPINSKKKKQCQTLIILSNHIFTNLKLNLF
CCCCCCCCCCCCCHHHEEEEEEECCCEEEEECCCCCHHHHHHEEEEEECCCEEEEEHHHH
THQLSNCLLAYKINYLLSPQKSKIFLIENLDNIKQTYKNLPLKNFQELKMDWRQIAPHRI
HHHHHHHHEEEEEHHHCCCCCCEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
PEIKQKVIQAVLEKKITNELEELKAFVFNHKHNEKVDAWHKKEKKRKKYDKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKNLSSLGKIKKAQKIIQIFKQKGFEAFIVGGAVRDYLLNIPFNQDVDITTNAVPQDINA
CCCHHHHHHHHHHHHHHHHHHHCCCEEEEECHHHHHHHEECCCCCCCCCCCCCCCHHHHH
IFQSQKRAYYGCIKIIFEEESFEITTYREEDIYLNHRHPNKIVFIQEVKKDVIRRDFTIN
HHHHHHHHHEEEEEEEEECCCEEEEEEECCCEEEECCCCCEEEEHHHHHHHHHHCCCEEE
ALLMDEKGQIFDWTEGKSDLTKKILRTIQNPFISFEKDALRIMRAFYFQAKLNFQIEKQT
EEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHEEEEEEECCC
QIALTKHSHLLTQISSSRIYEYLQKIITYPHWQASFKMMIETKAHLFLKPITKAIIFFAS
EEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEEHHHHHHHHHHHHHHHHHHHH
LKDEEFTNKNLEENIFWSIAFVFNKNIFSLFPINSKKKKQCQTLIILSNHIFTNLKLNLF
CCCCCCCCCCCCCHHHEEEEEEECCCEEEEECCCCCHHHHHHEEEEEECCCEEEEEHHHH
THQLSNCLLAYKINYLLSPQKSKIFLIENLDNIKQTYKNLPLKNFQELKMDWRQIAPHRI
HHHHHHHHEEEEEHHHCCCCCCEEEEEECHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCC
PEIKQKVIQAVLEKKITNELEELKAFVFNHKHNEKVDAWHKKEKKRKKYDKQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA