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The map label for this gene is pdhB [H]

Identifier: 197294721

GI number: 197294721

Start: 696262

End: 697239

Strand: Direct

Name: pdhB [H]

Synonym: PAa_0687

Alternate gene names: 197294721

Gene position: 696262-697239 (Clockwise)

Preceding gene: 197294720

Following gene: 197294722

Centisome position: 79.12

GC content: 36.61

Gene sequence:

>978_bases
ATGGCTTTGATGACTTTATTACAAGCAATTAACCAAACTTTAGATAGTCAATTAAAAAAAGACCCCAATATGGTTGTTTT
TGGTCAAGATGTAGGTAAATTAGGAGGAGTTTTTCGCGTTACTCAAGGATTACAAACTAAATACGGCGAAAATCGTGTTT
TTAACACTCCTATTGCTGAATCAGCTATTATCGGAAGTGCCATTGGAATGGCAATGAACGGCTTAAAGCCAGTTGCAGAA
ATTCAATTTGACGGCTTTATTTTTGTAGGTCTGGAAGATTTATTCGCTCATGCAGCTCGTATGCGTAATCGTAGTCGCGG
TACTCGCAGTGTTCCTATGGTAGTTAGAGTTCCTGTAGGTGGTGGGGTAAAATCTTTAGAACATCACTCTGAAAGTTTAG
AAGTTATTTTAGGTTCTGTTCCTGGATTAAAAGTGGTAATTCCTTCTAACCCTTATGATGCCAAAGGTTTATTAATGGCA
GCTATCAAAGACCCTGACCCGGTTATTTTTATGGAACCTAAAAGAATTTACCGCGGATTTAAACAAGAAGTGCCAGAACA
AGATTATGAAGTTGAAATCGGTAAAGCTAAAATAGTTCAAGAAGGTTCAGATATTACCGTGGTTGCTTGGGGGGCGATGG
TTCCAGAAACTCAATTAGCAATTAAACAAATTAATAATGAAGTTTCAGTTGAACTTATTGATTTAAGAAGCATTAACCCT
ATTGACCGCGAAACTGTTATTGAATCAGTTAAAAAAACAGGTCGTTTTTTGGTAGTTCATGAAGCTTGCAAAACTTATGG
ACCTGCTGGTGAACTAATCACTTTAGTTAATGAAAAAGCTTTTTTACATTTAGAAGCCGCTCCATCAAGAGTTACTGGCA
ACGATATCACAATGCCTTTAGCCAAAGGAGAACATTATCAATTTTTAAGTCCTGAAAAAATAGCTGCTGCTATTCGCAAA
GTAGCTTTAGAAGAATAA

Upstream 100 bases:

>100_bases
GAGAAATTTTTGAATACACTTATGAAAAAATGACTCCTCAATTAGAAGAACAATACCAAGAATGCCAAGATTTTTTCAAT
CAGAAAGAAGGTAAATAATC

Downstream 100 bases:

>100_bases
ACATAACAAGGAGAAAAAATATGTTTGAATTTAAATTTGCTGATGTTGGAGAAGGTATTCATGAAGGAACCATTACAAGA
TGGTTTTTTAAAAAAGGCGA

Product: Pyruvate dehydrogenase E1 comp, beta subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MALMTLLQAINQTLDSQLKKDPNMVVFGQDVGKLGGVFRVTQGLQTKYGENRVFNTPIAESAIIGSAIGMAMNGLKPVAE
IQFDGFIFVGLEDLFAHAARMRNRSRGTRSVPMVVRVPVGGGVKSLEHHSESLEVILGSVPGLKVVIPSNPYDAKGLLMA
AIKDPDPVIFMEPKRIYRGFKQEVPEQDYEVEIGKAKIVQEGSDITVVAWGAMVPETQLAIKQINNEVSVELIDLRSINP
IDRETVIESVKKTGRFLVVHEACKTYGPAGELITLVNEKAFLHLEAAPSRVTGNDITMPLAKGEHYQFLSPEKIAAAIRK
VALEE

Sequences:

>Translated_325_residues
MALMTLLQAINQTLDSQLKKDPNMVVFGQDVGKLGGVFRVTQGLQTKYGENRVFNTPIAESAIIGSAIGMAMNGLKPVAE
IQFDGFIFVGLEDLFAHAARMRNRSRGTRSVPMVVRVPVGGGVKSLEHHSESLEVILGSVPGLKVVIPSNPYDAKGLLMA
AIKDPDPVIFMEPKRIYRGFKQEVPEQDYEVEIGKAKIVQEGSDITVVAWGAMVPETQLAIKQINNEVSVELIDLRSINP
IDRETVIESVKKTGRFLVVHEACKTYGPAGELITLVNEKAFLHLEAAPSRVTGNDITMPLAKGEHYQFLSPEKIAAAIRK
VALEE
>Mature_324_residues
ALMTLLQAINQTLDSQLKKDPNMVVFGQDVGKLGGVFRVTQGLQTKYGENRVFNTPIAESAIIGSAIGMAMNGLKPVAEI
QFDGFIFVGLEDLFAHAARMRNRSRGTRSVPMVVRVPVGGGVKSLEHHSESLEVILGSVPGLKVVIPSNPYDAKGLLMAA
IKDPDPVIFMEPKRIYRGFKQEVPEQDYEVEIGKAKIVQEGSDITVVAWGAMVPETQLAIKQINNEVSVELIDLRSINPI
DRETVIESVKKTGRFLVVHEACKTYGPAGELITLVNEKAFLHLEAAPSRVTGNDITMPLAKGEHYQFLSPEKIAAAIRKV
ALEE

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4557353, Length=299, Percent_Identity=44.8160535117057, Blast_Score=260, Evalue=9e-70,
Organism=Homo sapiens, GI34101272, Length=299, Percent_Identity=44.8160535117057, Blast_Score=260, Evalue=9e-70,
Organism=Homo sapiens, GI156564403, Length=304, Percent_Identity=36.8421052631579, Blast_Score=195, Evalue=4e-50,
Organism=Homo sapiens, GI291084858, Length=304, Percent_Identity=35.1973684210526, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI17506935, Length=324, Percent_Identity=40.7407407407407, Blast_Score=220, Evalue=9e-58,
Organism=Caenorhabditis elegans, GI17538422, Length=323, Percent_Identity=35.6037151702786, Blast_Score=188, Evalue=3e-48,
Organism=Saccharomyces cerevisiae, GI6319698, Length=325, Percent_Identity=37.2307692307692, Blast_Score=206, Evalue=4e-54,
Organism=Drosophila melanogaster, GI160714828, Length=300, Percent_Identity=42.6666666666667, Blast_Score=244, Evalue=8e-65,
Organism=Drosophila melanogaster, GI160714832, Length=300, Percent_Identity=42.6666666666667, Blast_Score=243, Evalue=9e-65,
Organism=Drosophila melanogaster, GI21358145, Length=304, Percent_Identity=36.5131578947368, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24650940, Length=304, Percent_Identity=36.5131578947368, Blast_Score=205, Evalue=3e-53,
Organism=Drosophila melanogaster, GI24650943, Length=83, Percent_Identity=40.9638554216867, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24650945, Length=83, Percent_Identity=40.9638554216867, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: =1.2.4.1 [H]

Molecular weight: Translated: 35513; Mature: 35382

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALMTLLQAINQTLDSQLKKDPNMVVFGQDVGKLGGVFRVTQGLQTKYGENRVFNTPIAE
CHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHCCCCCEECCCCHH
SAIIGSAIGMAMNGLKPVAEIQFDGFIFVGLEDLFAHAARMRNRSRGTRSVPMVVRVPVG
HHHHHHHHHHHHCCCCCCEEEEECCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
GGVKSLEHHSESLEVILGSVPGLKVVIPSNPYDAKGLLMAAIKDPDPVIFMEPKRIYRGF
CCHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCCCEEEEEECCCCCEEEECHHHHHHHH
KQEVPEQDYEVEIGKAKIVQEGSDITVVAWGAMVPETQLAIKQINNEVSVELIDLRSINP
HHHCCCCCCEEEECCEEEEECCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCCC
IDRETVIESVKKTGRFLVVHEACKTYGPAGELITLVNEKAFLHLEAAPSRVTGNDITMPL
CCHHHHHHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCEEEEEECCCCCCCCCCEEEEE
AKGEHYQFLSPEKIAAAIRKVALEE
CCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALMTLLQAINQTLDSQLKKDPNMVVFGQDVGKLGGVFRVTQGLQTKYGENRVFNTPIAE
HHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHCCCCCEECCCCHH
SAIIGSAIGMAMNGLKPVAEIQFDGFIFVGLEDLFAHAARMRNRSRGTRSVPMVVRVPVG
HHHHHHHHHHHHCCCCCCEEEEECCEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEEECC
GGVKSLEHHSESLEVILGSVPGLKVVIPSNPYDAKGLLMAAIKDPDPVIFMEPKRIYRGF
CCHHHHHHHHHHHHEEEECCCCEEEEECCCCCCCCCEEEEEECCCCCEEEECHHHHHHHH
KQEVPEQDYEVEIGKAKIVQEGSDITVVAWGAMVPETQLAIKQINNEVSVELIDLRSINP
HHHCCCCCCEEEECCEEEEECCCCEEEEEECCCCCHHHHHHHHCCCCEEEEEEEECCCCC
IDRETVIESVKKTGRFLVVHEACKTYGPAGELITLVNEKAFLHLEAAPSRVTGNDITMPL
CCHHHHHHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCEEEEEECCCCCCCCCCEEEEE
AKGEHYQFLSPEKIAAAIRKVALEE
CCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1735725 [H]