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The map label for this gene is topA [H]

Identifier: 197294676

GI number: 197294676

Start: 632050

End: 634017

Strand: Reverse

Name: topA [H]

Synonym: PAa_0634

Alternate gene names: 197294676

Gene position: 634017-632050 (Counterclockwise)

Preceding gene: 197294677

Following gene: 197294674

Centisome position: 72.05

GC content: 27.79

Gene sequence:

>1968_bases
ATGAAAAAAAAAGTTATTATAGTTGAGTCTCCTGCCAAAATTAAAACATTAAGCCGTTTTTTTGATGATAAAGTGGAAAT
TTTATCTTCCAAAGGTCATATTAGAGATTTATCTTTATCAGGAAAAGATAGACTTGGAATTGACATTAAAAATGGTTTTA
TTCCTAAATATGAAATTATTAAAGAAAAAAAAACTTTAGTTGAGTCTTTGATTAAAAAAACCAAAAATAAAGAAATTTTT
TTAGCAACTGACCCCGATAGAGAAGGTGAAGCGATTGCTTGGCATTTATCAAAAGTTTTGAATTTAAATCCTCAAATGGC
TAATAGAATTGTTTTTCGTGAAATCACTAAAGAAGTAGTTTTAAAAGCTTTGCAAAATCCTCGTACAGTTGATGAATTAT
TAGTTTTTTCTCAAGAAACTAGAAGGATGCTTGACCGTATTATCGGATTTAAGTTGTCACGCCTAGTTAGAAGAATTAAA
TCTCAGTCTGCAGGAAGAGTTCAATCGGTTGCTTTAAAATTAATTGTTGATTTAGAAAAAGAAATTAATTCTTTTGTTCC
TGAAGAATACAATTTAATTACTGCTTTTTTTCCATTTTTCCAAGCCGAATACCAAAACCCTAATAACAAAAAAATTAAAT
CTGCAGAAGCTTTAGGCATTGTTGCAAAAATAACCAACAAACCTTTTATAGTCAAAAAAATCAAACAAACAGAAACATTT
TCTAAACCAAAAAAACCATTGATTACTTCGACTTTGCAACAAGAAGCAATTAATAATTTATATATGACTTCTAATCAAAC
AATGAGAGTAGCTCAAAAACTTTATGAAGGGATTGATATTGAAGGAGAATCCATTGGATTAATCACTTATATGCGTACTG
ATTCTCCTAGGTTTTCAGAAGTATTTGTAAAAGATGCTCAAAAATTAATTAAGGAAAAATATGGTCAAAAATATTTAGCA
GTTTTTAAACAAAATCAAAAAAACAAGGCCCAAGATGCTCATGAAGCAATTAAGCCTACGGATTTATCAAAAACACCAGA
AAGTTTAATGTCATATCTTGATAAATATGAATATAAACTTTATGATCTTATTTACAAAAGATCATTGGCAAGTTTGATGA
AACCGGCTATATTTCAAAAAAACCAAGTTTTTTTTGAAGTTGAAAAACATTCTTTTTTAACTGAAGGGTTAATTAAAACA
TTTGATGGTTATCAAAAAATTTTAAAAGACAACCTTCAAGACAAAATTATTCCAACTTTTAATTTAAATGAAAGTTATTT
TCCTGAAAAAATAGAGACTTTTCAAAAGTTTACTACTCCACAAGCTCGATTTTCTGAAGCCACTTTAATTAAAACTTTAG
AAAAACTGAACATTGGAAGGCCTTCCACTTATTCGCAAATTATTTTTACTCTCAAAAAAAGATTTTATGTTGATATGATC
GAAAAACGTTTTCAACCCACAGAACAAGGCATTTTAACCATTCAAAACTTAGAACTTTTTTTTAAGCAATTCCTTGATTA
TCAATATACGGCACAAATGGAGTCCGATTTAGATAAAATTGCTTCAGGATTAGTAGATAATAAACAATTAATGCAAACTT
TTTATACAAATTTTTTGCAACTTTATCAAATAGCAGATCAAAAACTCGAAAAAATAAAACCTATTGAAACCGATCAAAAA
TGTAATTTATGTAATGAAGGTAATTTATTAATTAAAAAAAGTCATTATGGTTCGTTTTTGGGTTGCAGCCGTTTTCCTGA
TTGCAAAAACACTTCTCCTTGGAAAGTTCAAGAAAAAAATAAATCAAAAGATTCCGATCATTCAAACCAAACCGATCAAA
AATGTAATTTATGTAATGAAGGTAATTTATTAATTAAAAAAAGTCGTTATGGTTCGTTTTTGGGTTGCAGCCGTTTTCCT
GATTGCAAAAACATTGTTAAAATAAAAAATAAACCAAATAGCAAATAA

Upstream 100 bases:

>100_bases
GATTAGGAAAAATGAATTTTGATTTAGATATTTTGCCTTCTTTAGATGATTTTTTTCAAAAACAGATGAAATTAAGTTAA
ATACTTTTACGAGGAATTAA

Downstream 100 bases:

>100_bases
TTGTTTAAGTATTCCTTTTTTTAGATAAACTTTATAATTTTAATCACTCAAGTTTAAAATCAGAGATATTTTTCCATATC
TTTTCAGAATAAAAAATTAA

Product: Topoisomerase IA

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 655; Mature: 655

Protein sequence:

>655_residues
MKKKVIIVESPAKIKTLSRFFDDKVEILSSKGHIRDLSLSGKDRLGIDIKNGFIPKYEIIKEKKTLVESLIKKTKNKEIF
LATDPDREGEAIAWHLSKVLNLNPQMANRIVFREITKEVVLKALQNPRTVDELLVFSQETRRMLDRIIGFKLSRLVRRIK
SQSAGRVQSVALKLIVDLEKEINSFVPEEYNLITAFFPFFQAEYQNPNNKKIKSAEALGIVAKITNKPFIVKKIKQTETF
SKPKKPLITSTLQQEAINNLYMTSNQTMRVAQKLYEGIDIEGESIGLITYMRTDSPRFSEVFVKDAQKLIKEKYGQKYLA
VFKQNQKNKAQDAHEAIKPTDLSKTPESLMSYLDKYEYKLYDLIYKRSLASLMKPAIFQKNQVFFEVEKHSFLTEGLIKT
FDGYQKILKDNLQDKIIPTFNLNESYFPEKIETFQKFTTPQARFSEATLIKTLEKLNIGRPSTYSQIIFTLKKRFYVDMI
EKRFQPTEQGILTIQNLELFFKQFLDYQYTAQMESDLDKIASGLVDNKQLMQTFYTNFLQLYQIADQKLEKIKPIETDQK
CNLCNEGNLLIKKSHYGSFLGCSRFPDCKNTSPWKVQEKNKSKDSDHSNQTDQKCNLCNEGNLLIKKSRYGSFLGCSRFP
DCKNIVKIKNKPNSK

Sequences:

>Translated_655_residues
MKKKVIIVESPAKIKTLSRFFDDKVEILSSKGHIRDLSLSGKDRLGIDIKNGFIPKYEIIKEKKTLVESLIKKTKNKEIF
LATDPDREGEAIAWHLSKVLNLNPQMANRIVFREITKEVVLKALQNPRTVDELLVFSQETRRMLDRIIGFKLSRLVRRIK
SQSAGRVQSVALKLIVDLEKEINSFVPEEYNLITAFFPFFQAEYQNPNNKKIKSAEALGIVAKITNKPFIVKKIKQTETF
SKPKKPLITSTLQQEAINNLYMTSNQTMRVAQKLYEGIDIEGESIGLITYMRTDSPRFSEVFVKDAQKLIKEKYGQKYLA
VFKQNQKNKAQDAHEAIKPTDLSKTPESLMSYLDKYEYKLYDLIYKRSLASLMKPAIFQKNQVFFEVEKHSFLTEGLIKT
FDGYQKILKDNLQDKIIPTFNLNESYFPEKIETFQKFTTPQARFSEATLIKTLEKLNIGRPSTYSQIIFTLKKRFYVDMI
EKRFQPTEQGILTIQNLELFFKQFLDYQYTAQMESDLDKIASGLVDNKQLMQTFYTNFLQLYQIADQKLEKIKPIETDQK
CNLCNEGNLLIKKSHYGSFLGCSRFPDCKNTSPWKVQEKNKSKDSDHSNQTDQKCNLCNEGNLLIKKSRYGSFLGCSRFP
DCKNIVKIKNKPNSK
>Mature_655_residues
MKKKVIIVESPAKIKTLSRFFDDKVEILSSKGHIRDLSLSGKDRLGIDIKNGFIPKYEIIKEKKTLVESLIKKTKNKEIF
LATDPDREGEAIAWHLSKVLNLNPQMANRIVFREITKEVVLKALQNPRTVDELLVFSQETRRMLDRIIGFKLSRLVRRIK
SQSAGRVQSVALKLIVDLEKEINSFVPEEYNLITAFFPFFQAEYQNPNNKKIKSAEALGIVAKITNKPFIVKKIKQTETF
SKPKKPLITSTLQQEAINNLYMTSNQTMRVAQKLYEGIDIEGESIGLITYMRTDSPRFSEVFVKDAQKLIKEKYGQKYLA
VFKQNQKNKAQDAHEAIKPTDLSKTPESLMSYLDKYEYKLYDLIYKRSLASLMKPAIFQKNQVFFEVEKHSFLTEGLIKT
FDGYQKILKDNLQDKIIPTFNLNESYFPEKIETFQKFTTPQARFSEATLIKTLEKLNIGRPSTYSQIIFTLKKRFYVDMI
EKRFQPTEQGILTIQNLELFFKQFLDYQYTAQMESDLDKIASGLVDNKQLMQTFYTNFLQLYQIADQKLEKIKPIETDQK
CNLCNEGNLLIKKSHYGSFLGCSRFPDCKNTSPWKVQEKNKSKDSDHSNQTDQKCNLCNEGNLLIKKSRYGSFLGCSRFP
DCKNIVKIKNKPNSK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=580, Percent_Identity=26.7241379310345, Blast_Score=152, Evalue=9e-37,
Organism=Homo sapiens, GI4507635, Length=493, Percent_Identity=22.7180527383367, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI1787529, Length=636, Percent_Identity=35.2201257861635, Blast_Score=351, Evalue=7e-98,
Organism=Escherichia coli, GI1788061, Length=533, Percent_Identity=24.390243902439, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17555378, Length=455, Percent_Identity=25.2747252747253, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24585251, Length=703, Percent_Identity=25.0355618776671, Blast_Score=147, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 75974; Mature: 75974

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKVIIVESPAKIKTLSRFFDDKVEILSSKGHIRDLSLSGKDRLGIDIKNGFIPKYEII
CCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCCCCHHHHH
KEKKTLVESLIKKTKNKEIFLATDPDREGEAIAWHLSKVLNLNPQMANRIVFREITKEVV
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LKALQNPRTVDELLVFSQETRRMLDRIIGFKLSRLVRRIKSQSAGRVQSVALKLIVDLEK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EINSFVPEEYNLITAFFPFFQAEYQNPNNKKIKSAEALGIVAKITNKPFIVKKIKQTETF
HHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SKPKKPLITSTLQQEAINNLYMTSNQTMRVAQKLYEGIDIEGESIGLITYMRTDSPRFSE
CCCCCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHH
VFVKDAQKLIKEKYGQKYLAVFKQNQKNKAQDAHEAIKPTDLSKTPESLMSYLDKYEYKL
HHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
YDLIYKRSLASLMKPAIFQKNQVFFEVEKHSFLTEGLIKTFDGYQKILKDNLQDKIIPTF
HHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCC
NLNESYFPEKIETFQKFTTPQARFSEATLIKTLEKLNIGRPSTYSQIIFTLKKRFYVDMI
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EKRFQPTEQGILTIQNLELFFKQFLDYQYTAQMESDLDKIASGLVDNKQLMQTFYTNFLQ
HHHCCCCHHCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LYQIADQKLEKIKPIETDQKCNLCNEGNLLIKKSHYGSFLGCSRFPDCKNTSPWKVQEKN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCCC
KSKDSDHSNQTDQKCNLCNEGNLLIKKSRYGSFLGCSRFPDCKNIVKIKNKPNSK
CCCCCCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHEECCCCCCC
>Mature Secondary Structure
MKKKVIIVESPAKIKTLSRFFDDKVEILSSKGHIRDLSLSGKDRLGIDIKNGFIPKYEII
CCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCEEECCCCCCHHHHH
KEKKTLVESLIKKTKNKEIFLATDPDREGEAIAWHLSKVLNLNPQMANRIVFREITKEVV
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LKALQNPRTVDELLVFSQETRRMLDRIIGFKLSRLVRRIKSQSAGRVQSVALKLIVDLEK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
EINSFVPEEYNLITAFFPFFQAEYQNPNNKKIKSAEALGIVAKITNKPFIVKKIKQTETF
HHHHHCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
SKPKKPLITSTLQQEAINNLYMTSNQTMRVAQKLYEGIDIEGESIGLITYMRTDSPRFSE
CCCCCCHHHHHHHHHHHHHEEECCCHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCHHHH
VFVKDAQKLIKEKYGQKYLAVFKQNQKNKAQDAHEAIKPTDLSKTPESLMSYLDKYEYKL
HHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
YDLIYKRSLASLMKPAIFQKNQVFFEVEKHSFLTEGLIKTFDGYQKILKDNLQDKIIPTF
HHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCC
NLNESYFPEKIETFQKFTTPQARFSEATLIKTLEKLNIGRPSTYSQIIFTLKKRFYVDMI
CCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
EKRFQPTEQGILTIQNLELFFKQFLDYQYTAQMESDLDKIASGLVDNKQLMQTFYTNFLQ
HHHCCCCHHCCEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LYQIADQKLEKIKPIETDQKCNLCNEGNLLIKKSHYGSFLGCSRFPDCKNTSPWKVQEKN
HHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCEECCCC
KSKDSDHSNQTDQKCNLCNEGNLLIKKSRYGSFLGCSRFPDCKNIVKIKNKPNSK
CCCCCCCCCCCHHHCCCCCCCCEEEEECCCCCCCCCCCCCCCHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA