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The map label for this gene is infB [H]

Identifier: 197294652

GI number: 197294652

Start: 602491

End: 604362

Strand: Direct

Name: infB [H]

Synonym: PAa_0608

Alternate gene names: 197294652

Gene position: 602491-604362 (Clockwise)

Preceding gene: 197294651

Following gene: 197294653

Centisome position: 68.47

GC content: 30.5

Gene sequence:

>1872_bases
ATGACCCCTGATAAAAATAAAAATAAACTGGAAGTTGATAAAAATAAAGAACAATTAGATCCTCAAAAAAATTCCAAAAA
AATGGTTCATAAGACTTATTTTTTCAACCCTCAAGATAAAGTTTTTCAAATAGCTCAATCTTTTGAGGTTTCTAGTGCTT
CTTTAATTCAAAAATTATTGCAATTAGGAATTAAAACAGACGTTAATCAAACTTTAAATAAAGAAACAATTAAAATTTTG
GCCAATACTTGTAACGTAGAATTTTTAGAAAAACCCGAAGAAAATAATGTATCAAAAAATCAAGAACATAAAGAAAAAAA
TCCTGTAGTTAAAAAAGAATCTAGAACAAAAGAAACAAAAACCAATTTAGAAAAAATACCTCCAATCGTAGTAATTATGG
GCCATGTTGATCATGGAAAAACCACTTTATTAGATGCAATTCGCAAAACTCGCGTTGTTGATCAAGAATTTGGAGGAATC
ACTCAACACATTGGCGCATATCAGGTGGATTATCAAAATAATAAAATAACTTTCATTGATACCCCAGGTCATGAAGCTTT
TGGTAAAATGAGAGCTAGAGGAGCTCAAATAACAAATATTTGTATTTTAGTTGTTGCCGTTGATGATTGTGTAAAACCTC
AAACGATTGAAGCTTTGAAACACGCCAAAAAAGCCCAAATACCTATTATTGTTGCTCTCAATAAATCAGATAAACCCAAT
AATAATAGTAAGCAAATTATGACAGAGTTGTCTAGTTTTGATTTATTGCCTGAAGAATGGGGCGGGCAAACCCCTTATAT
ACCTATTTCAGCCCTCAAAAAAGAAGGACTTGATAAATTATTAGAAACCATTTTGCTTTTAAGTGAAATTCAAGATCTAA
AAGCAGATTTGCAAAAAAAAGGCCAAGGAGCAGTTCTTGAGGCTAGTCTAGATAAGTCTTTAGGACCTGTTGCCACTTTT
ATGGTTAATTACGGCAATTTAAAAATAAGCGATGTGGTAGTTGTGGGTCATACTTATGGCAAAATTCGTTTTATGGAAGA
TGAAAACAAAAAGCCATTAAAAAAAGTTTTGCCTTCTCAACCCGTCAGAGTTGCAGGGCTAAAAGACGTTCCTAAAGCAG
GCGACCTTTTCTACGTTGTTGCTAACGAAAAAGAAGCTCGAAAAATTATTTTAGAAGCAAAATCCGAGCAACAAGAAAAC
TTATCTAAAGCTTTACCATCGCTAGACTTAGAAAATATTTTACAAAACCTTGAAGAAGAAAAACCTAAAGAACTTAACAT
TATTTTAAAAACAGATACTCAAGGCAGTTTAGAAGCTATTTGCGGGATGATTGAAAAAATTAATGTTTCTGACTTGAAAG
TTAATCTTTTACGCACTTCCGTTGGAACTGTTACTGAAAAAGATGTTATTTTAGCTGAATCCTCGCAATCCCTTTTAATC
GGTTTTAACGTTAAACCTTCATCTTCTATTTTAAAATTAGCTCAAAAGCAAAAAATTCAAATCGTTGTTCATAATATTAT
TTATCGTATTATTGAAGATATTGAAAAAAGATTAAAACAAATGGTTAAACCTGTTTTTGAAGAAGTTTTTACCGGACAAG
CAGAAGTAAGAAAAATCTTTCATATTAGTAAGATTGGCAATATTGCTGGTTGTTATGTCACTCAAGGAATTGTTAACAAT
AATGATTTTGCTAAAGTAATCCGAGATGGCGAAGTTATCTTTAAAGGCAAAATTAACTCCTTAAAACGTTTAAAAGAAAA
CATTAAATCTTCTAAACAAGGTTATGAATGTGGTATTTTGCTTGATAATTTTAATGATTTTATAATTAATGATATAATAG
AAACATCTAAATTAGACAAAATGGAGAATTGA

Upstream 100 bases:

>100_bases
TAAAATTTGTTTTATCAAAAAATAACTTAAAAAAAGAAGCTGATAATTTAAATAATTAAATAATTAATATTTTTCATGTA
AATAAAAAAGGAGGGATTAA

Downstream 100 bases:

>100_bases
AAATGACTGCAACAACAGCTGAAAGAATGGCAAGCGCTATTAAAAAAGAGTTAGTTTTAATTAATACAATTATTCAAGAC
GAACAAATAGGTTATATTAA

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 623; Mature: 622

Protein sequence:

>623_residues
MTPDKNKNKLEVDKNKEQLDPQKNSKKMVHKTYFFNPQDKVFQIAQSFEVSSASLIQKLLQLGIKTDVNQTLNKETIKIL
ANTCNVEFLEKPEENNVSKNQEHKEKNPVVKKESRTKETKTNLEKIPPIVVIMGHVDHGKTTLLDAIRKTRVVDQEFGGI
TQHIGAYQVDYQNNKITFIDTPGHEAFGKMRARGAQITNICILVVAVDDCVKPQTIEALKHAKKAQIPIIVALNKSDKPN
NNSKQIMTELSSFDLLPEEWGGQTPYIPISALKKEGLDKLLETILLLSEIQDLKADLQKKGQGAVLEASLDKSLGPVATF
MVNYGNLKISDVVVVGHTYGKIRFMEDENKKPLKKVLPSQPVRVAGLKDVPKAGDLFYVVANEKEARKIILEAKSEQQEN
LSKALPSLDLENILQNLEEEKPKELNIILKTDTQGSLEAICGMIEKINVSDLKVNLLRTSVGTVTEKDVILAESSQSLLI
GFNVKPSSSILKLAQKQKIQIVVHNIIYRIIEDIEKRLKQMVKPVFEEVFTGQAEVRKIFHISKIGNIAGCYVTQGIVNN
NDFAKVIRDGEVIFKGKINSLKRLKENIKSSKQGYECGILLDNFNDFIINDIIETSKLDKMEN

Sequences:

>Translated_623_residues
MTPDKNKNKLEVDKNKEQLDPQKNSKKMVHKTYFFNPQDKVFQIAQSFEVSSASLIQKLLQLGIKTDVNQTLNKETIKIL
ANTCNVEFLEKPEENNVSKNQEHKEKNPVVKKESRTKETKTNLEKIPPIVVIMGHVDHGKTTLLDAIRKTRVVDQEFGGI
TQHIGAYQVDYQNNKITFIDTPGHEAFGKMRARGAQITNICILVVAVDDCVKPQTIEALKHAKKAQIPIIVALNKSDKPN
NNSKQIMTELSSFDLLPEEWGGQTPYIPISALKKEGLDKLLETILLLSEIQDLKADLQKKGQGAVLEASLDKSLGPVATF
MVNYGNLKISDVVVVGHTYGKIRFMEDENKKPLKKVLPSQPVRVAGLKDVPKAGDLFYVVANEKEARKIILEAKSEQQEN
LSKALPSLDLENILQNLEEEKPKELNIILKTDTQGSLEAICGMIEKINVSDLKVNLLRTSVGTVTEKDVILAESSQSLLI
GFNVKPSSSILKLAQKQKIQIVVHNIIYRIIEDIEKRLKQMVKPVFEEVFTGQAEVRKIFHISKIGNIAGCYVTQGIVNN
NDFAKVIRDGEVIFKGKINSLKRLKENIKSSKQGYECGILLDNFNDFIINDIIETSKLDKMEN
>Mature_622_residues
TPDKNKNKLEVDKNKEQLDPQKNSKKMVHKTYFFNPQDKVFQIAQSFEVSSASLIQKLLQLGIKTDVNQTLNKETIKILA
NTCNVEFLEKPEENNVSKNQEHKEKNPVVKKESRTKETKTNLEKIPPIVVIMGHVDHGKTTLLDAIRKTRVVDQEFGGIT
QHIGAYQVDYQNNKITFIDTPGHEAFGKMRARGAQITNICILVVAVDDCVKPQTIEALKHAKKAQIPIIVALNKSDKPNN
NSKQIMTELSSFDLLPEEWGGQTPYIPISALKKEGLDKLLETILLLSEIQDLKADLQKKGQGAVLEASLDKSLGPVATFM
VNYGNLKISDVVVVGHTYGKIRFMEDENKKPLKKVLPSQPVRVAGLKDVPKAGDLFYVVANEKEARKIILEAKSEQQENL
SKALPSLDLENILQNLEEEKPKELNIILKTDTQGSLEAICGMIEKINVSDLKVNLLRTSVGTVTEKDVILAESSQSLLIG
FNVKPSSSILKLAQKQKIQIVVHNIIYRIIEDIEKRLKQMVKPVFEEVFTGQAEVRKIFHISKIGNIAGCYVTQGIVNNN
DFAKVIRDGEVIFKGKINSLKRLKENIKSSKQGYECGILLDNFNDFIINDIIETSKLDKMEN

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=529, Percent_Identity=39.8865784499055, Blast_Score=359, Evalue=5e-99,
Organism=Homo sapiens, GI53729337, Length=529, Percent_Identity=39.8865784499055, Blast_Score=359, Evalue=5e-99,
Organism=Homo sapiens, GI84043963, Length=473, Percent_Identity=27.6955602536998, Blast_Score=115, Evalue=9e-26,
Organism=Escherichia coli, GI1789559, Length=575, Percent_Identity=41.0434782608696, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI2367247, Length=208, Percent_Identity=32.2115384615385, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI71994658, Length=525, Percent_Identity=36.7619047619048, Blast_Score=316, Evalue=2e-86,
Organism=Caenorhabditis elegans, GI212656558, Length=558, Percent_Identity=26.3440860215054, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=369, Percent_Identity=23.8482384823848, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6324550, Length=521, Percent_Identity=39.3474088291747, Blast_Score=330, Evalue=5e-91,
Organism=Saccharomyces cerevisiae, GI6319282, Length=464, Percent_Identity=25.4310344827586, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI28572034, Length=597, Percent_Identity=34.6733668341709, Blast_Score=321, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24656849, Length=577, Percent_Identity=25.3032928942808, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI160714833, Length=104, Percent_Identity=51.9230769230769, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 69922; Mature: 69791

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPDKNKNKLEVDKNKEQLDPQKNSKKMVHKTYFFNPQDKVFQIAQSFEVSSASLIQKLL
CCCCCCCCCEEECCCHHHCCCCCCCHHHHHHHEEECCHHHHHHHHHHCCCCHHHHHHHHH
QLGIKTDVNQTLNKETIKILANTCNVEFLEKPEENNVSKNQEHKEKNPVVKKESRTKETK
HHCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHCCCCCCCHHHHHHHH
TNLEKIPPIVVIMGHVDHGKTTLLDAIRKTRVVDQEFGGITQHIGAYQVDYQNNKITFID
HHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCEEEEEE
TPGHEAFGKMRARGAQITNICILVVAVDDCVKPQTIEALKHAKKAQIPIIVALNKSDKPN
CCCHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
NNSKQIMTELSSFDLLPEEWGGQTPYIPISALKKEGLDKLLETILLLSEIQDLKADLQKK
CCHHHHHHHHHHCCCCHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GQGAVLEASLDKSLGPVATFMVNYGNLKISDVVVVGHTYGKIRFMEDENKKPLKKVLPSQ
CCCCEEEECCCCCCCHHHHHEEECCCEEEEEEEEEECCCCEEEEECCCCCCHHHHHCCCC
PVRVAGLKDVPKAGDLFYVVANEKEARKIILEAKSEQQENLSKALPSLDLENILQNLEEE
CEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
KPKELNIILKTDTQGSLEAICGMIEKINVSDLKVNLLRTSVGTVTEKDVILAESSQSLLI
CCCEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEHHCCCCCCCCCEEEEECCCCEEE
GFNVKPSSSILKLAQKQKIQIVVHNIIYRIIEDIEKRLKQMVKPVFEEVFTGQAEVRKIF
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HISKIGNIAGCYVTQGIVNNNDFAKVIRDGEVIFKGKINSLKRLKENIKSSKQGYECGIL
HHHHHCCCHHHHHCCCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEEE
LDNFNDFIINDIIETSKLDKMEN
EECCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TPDKNKNKLEVDKNKEQLDPQKNSKKMVHKTYFFNPQDKVFQIAQSFEVSSASLIQKLL
CCCCCCCCEEECCCHHHCCCCCCCHHHHHHHEEECCHHHHHHHHHHCCCCHHHHHHHHH
QLGIKTDVNQTLNKETIKILANTCNVEFLEKPEENNVSKNQEHKEKNPVVKKESRTKETK
HHCCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHCCCCCCCHHHHHHHH
TNLEKIPPIVVIMGHVDHGKTTLLDAIRKTRVVDQEFGGITQHIGAYQVDYQNNKITFID
HHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCEEEEECCCEEEEEE
TPGHEAFGKMRARGAQITNICILVVAVDDCVKPQTIEALKHAKKAQIPIIVALNKSDKPN
CCCHHHHHHHHHCCCCCEEEEEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
NNSKQIMTELSSFDLLPEEWGGQTPYIPISALKKEGLDKLLETILLLSEIQDLKADLQKK
CCHHHHHHHHHHCCCCHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GQGAVLEASLDKSLGPVATFMVNYGNLKISDVVVVGHTYGKIRFMEDENKKPLKKVLPSQ
CCCCEEEECCCCCCCHHHHHEEECCCEEEEEEEEEECCCCEEEEECCCCCCHHHHHCCCC
PVRVAGLKDVPKAGDLFYVVANEKEARKIILEAKSEQQENLSKALPSLDLENILQNLEEE
CEEECCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHC
KPKELNIILKTDTQGSLEAICGMIEKINVSDLKVNLLRTSVGTVTEKDVILAESSQSLLI
CCCEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEEEHHCCCCCCCCCEEEEECCCCEEE
GFNVKPSSSILKLAQKQKIQIVVHNIIYRIIEDIEKRLKQMVKPVFEEVFTGQAEVRKIF
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
HISKIGNIAGCYVTQGIVNNNDFAKVIRDGEVIFKGKINSLKRLKENIKSSKQGYECGIL
HHHHHCCCHHHHHCCCCCCCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCEEEEE
LDNFNDFIINDIIETSKLDKMEN
EECCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA