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The map label for this gene is nusA [H]

Identifier: 197294650

GI number: 197294650

Start: 601078

End: 602151

Strand: Direct

Name: nusA [H]

Synonym: PAa_0606

Alternate gene names: 197294650

Gene position: 601078-602151 (Clockwise)

Preceding gene: 197294649

Following gene: 197294651

Centisome position: 68.31

GC content: 28.86

Gene sequence:

>1074_bases
TTGATTAGTAAGGGATTTTTTAAATTTTTAGATAAGATATCACAAGAAAGGGATTTACCCAAAGAAGAAGTTGTTGAGGC
TTTTACTAAAGGTTTAATTGCTGGTTGTAAAAAAAATAACCGAGTTAAAAGTTGTCGTGTTCATTTAAAAGAAAACAAAA
ACGAAGTTCTTTTATATAAACAATTTATAGTTGTTGATGAAACTAATGCAAATACTATTAATTTAAAAGAATGGACTCCT
ATTAGTTTAGAAGAAGCGCAAAAAATTAAACTACATGCAAAAGTGGGCCAAGTCCTTGAAATAAAAGTCAATCCTAAAGA
TTTTAATTTATACGCCATTAAAGAATTTAAAAACCAGTTTAATGAAGAATTAACCAAAAGAAAAAGAGAAAGTGTTTATC
AATTTTTTAAACAACAGGAAGGTAAGTTAATTAGTGCTAAAGTTATTTCTGAAAACGAAAAATTTTATAATTTAGAGTTA
GAAAAAGAAATTGCTACTTTATTGCTGAAAAAAGAAACTTCTGGTAATAATTTGCCACATATTGGTGAAAGAATTCAAGT
TTTTTTGTTAGAAGTCAGAAAAACGAGTAAATGGCCTAAAATTTTTGTGAGTCATAATCATGTAGGACTTGTAGTTAAAG
TTTTTGAAGAAAATATTCCTGAAATACAAGAAGGGATTGTAAAAATTATGACAATTGCACGTATTTCAGGCGAACGAACA
AAAATTGGATTAGTTTCTTATGACCCCCAAGTTGATCCCATTGGTTCTTGCATTGGAGAAAACAGTGATCGCATTAGGAA
TATTATTAAAATTTTAAAAGGTGAAAAAATTGATTTGTTTCGTTGGAGCGAAGACGAAAAAGAATTGATTAGTAACGCTT
TAAGGCCTGCTCAATGCATTGATGTTATTATTAAAAATGACAGAAATAAGCATGCTTTGGCAATTATCCCTGATGATCAA
TTTTCTTTAGCTATCGGAAAATTGGGTAAAAATGTCAAATTAGCTGCTGAAGTTACCAAATGGAACATTGATATCAAAAC
CTTATCCCACGCTAAAAAAGAAGGAATAATATAA

Upstream 100 bases:

>100_bases
TTGAGGAATAAAAATATAGGAATAACGTTTTTTAGTTTCTATTTTTTAATTTTTATAGAATTTGGTTTTATTTTAAAAAT
CACAAAAGGAGAAACTTGTT

Downstream 100 bases:

>100_bases
TTAAGAAATGAATATTTTAAGAACTTGTATAGTAACTAAGAAAAGTAAAAACCCTCTTCAAATGATTAGAATTAACGTGA
ATAAATTAGGTCAAGTAGCA

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 357; Mature: 357

Protein sequence:

>357_residues
MISKGFFKFLDKISQERDLPKEEVVEAFTKGLIAGCKKNNRVKSCRVHLKENKNEVLLYKQFIVVDETNANTINLKEWTP
ISLEEAQKIKLHAKVGQVLEIKVNPKDFNLYAIKEFKNQFNEELTKRKRESVYQFFKQQEGKLISAKVISENEKFYNLEL
EKEIATLLLKKETSGNNLPHIGERIQVFLLEVRKTSKWPKIFVSHNHVGLVVKVFEENIPEIQEGIVKIMTIARISGERT
KIGLVSYDPQVDPIGSCIGENSDRIRNIIKILKGEKIDLFRWSEDEKELISNALRPAQCIDVIIKNDRNKHALAIIPDDQ
FSLAIGKLGKNVKLAAEVTKWNIDIKTLSHAKKEGII

Sequences:

>Translated_357_residues
MISKGFFKFLDKISQERDLPKEEVVEAFTKGLIAGCKKNNRVKSCRVHLKENKNEVLLYKQFIVVDETNANTINLKEWTP
ISLEEAQKIKLHAKVGQVLEIKVNPKDFNLYAIKEFKNQFNEELTKRKRESVYQFFKQQEGKLISAKVISENEKFYNLEL
EKEIATLLLKKETSGNNLPHIGERIQVFLLEVRKTSKWPKIFVSHNHVGLVVKVFEENIPEIQEGIVKIMTIARISGERT
KIGLVSYDPQVDPIGSCIGENSDRIRNIIKILKGEKIDLFRWSEDEKELISNALRPAQCIDVIIKNDRNKHALAIIPDDQ
FSLAIGKLGKNVKLAAEVTKWNIDIKTLSHAKKEGII
>Mature_357_residues
MISKGFFKFLDKISQERDLPKEEVVEAFTKGLIAGCKKNNRVKSCRVHLKENKNEVLLYKQFIVVDETNANTINLKEWTP
ISLEEAQKIKLHAKVGQVLEIKVNPKDFNLYAIKEFKNQFNEELTKRKRESVYQFFKQQEGKLISAKVISENEKFYNLEL
EKEIATLLLKKETSGNNLPHIGERIQVFLLEVRKTSKWPKIFVSHNHVGLVVKVFEENIPEIQEGIVKIMTIARISGERT
KIGLVSYDPQVDPIGSCIGENSDRIRNIIKILKGEKIDLFRWSEDEKELISNALRPAQCIDVIIKNDRNKHALAIIPDDQ
FSLAIGKLGKNVKLAAEVTKWNIDIKTLSHAKKEGII

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=350, Percent_Identity=25.4285714285714, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 40999; Mature: 40999

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISKGFFKFLDKISQERDLPKEEVVEAFTKGLIAGCKKNNRVKSCRVHLKENKNEVLLYK
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEE
QFIVVDETNANTINLKEWTPISLEEAQKIKLHAKVGQVLEIKVNPKDFNLYAIKEFKNQF
EEEEEECCCCCEEEECCCCCCCHHHHHHEEEEECCCCEEEEEECCCCCEEEEHHHHHHHH
NEELTKRKRESVYQFFKQQEGKLISAKVISENEKFYNLELEKEIATLLLKKETSGNNLPH
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHEECCCCCCCCC
IGERIQVFLLEVRKTSKWPKIFVSHNHVGLVVKVFEENIPEIQEGIVKIMTIARISGERT
HHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCE
KIGLVSYDPQVDPIGSCIGENSDRIRNIIKILKGEKIDLFRWSEDEKELISNALRPAQCI
EEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHHH
DVIIKNDRNKHALAIIPDDQFSLAIGKLGKNVKLAAEVTKWNIDIKTLSHAKKEGII
HHHHCCCCCCEEEEEECCCCHHHHHHHCCCCEEEEEEEEEEEEEHHHHHHHHHCCCC
>Mature Secondary Structure
MISKGFFKFLDKISQERDLPKEEVVEAFTKGLIAGCKKNNRVKSCRVHLKENKNEVLLYK
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEEE
QFIVVDETNANTINLKEWTPISLEEAQKIKLHAKVGQVLEIKVNPKDFNLYAIKEFKNQF
EEEEEECCCCCEEEECCCCCCCHHHHHHEEEEECCCCEEEEEECCCCCEEEEHHHHHHHH
NEELTKRKRESVYQFFKQQEGKLISAKVISENEKFYNLELEKEIATLLLKKETSGNNLPH
HHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEHHHHHHHHHHEECCCCCCCCC
IGERIQVFLLEVRKTSKWPKIFVSHNHVGLVVKVFEENIPEIQEGIVKIMTIARISGERT
HHHHHHHHHHHHHHCCCCCEEEEECCCCEEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCE
KIGLVSYDPQVDPIGSCIGENSDRIRNIIKILKGEKIDLFRWSEDEKELISNALRPAQCI
EEEEEEECCCCCHHHHHHCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCHHHHH
DVIIKNDRNKHALAIIPDDQFSLAIGKLGKNVKLAAEVTKWNIDIKTLSHAKKEGII
HHHHCCCCCCEEEEEECCCCHHHHHHHCCCCEEEEEEEEEEEEEHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]