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The map label for this gene is trmD

Identifier: 197294592

GI number: 197294592

Start: 555570

End: 556304

Strand: Reverse

Name: trmD

Synonym: PAa_0548

Alternate gene names: 197294592

Gene position: 556304-555570 (Counterclockwise)

Preceding gene: 197294593

Following gene: 197294591

Centisome position: 63.22

GC content: 29.93

Gene sequence:

>735_bases
ATGATTATTGAAATTGTAACTATTTTCCCACTATTTTTTAAAAGTTTTTGCGATAATTCAATCATAAAAAGAGCTTTAAA
TCAACAAAAAGTTCAAATAAAAATTCATGATTTAAGAAAATATAGCGTTAATAAACATCAACAAGTAGACGATTGTGTTT
ACGGAGGAGGTGTAGGAATGTTACTTTCTTTCCCTCCTTTTTTTGATTGTCTTCAAGAAATAAAAACTCCTCAAAGTAAA
GTTATTTTGTTATCTCCTCAAGGAAAAATTTTTGATCAAGTGCAAGCAAATAATTACGCTTCAAATACCCCTCATTTAAT
TATTTTATGTGGTAATTATGAAGGGGTTGACGCAAGAATTTTAAAATATGTTGATCAAGAAATTTCAATAGGTGATTATG
TTTTAACTGGAGGAGAAATCGCTGCCACTGTTGTTGTTGATGCTATTGTACGCCTTATTCCTGGGGTCATTAAACACGAA
TCTGCCTTTTCTGATTCGCACCAACAAGGTTTATTGAAGTTTCCTCAATACACAAGACCCCAAATTTATCAAAACCAAGA
AGTTCCTTCAGTTTTGTTGTCTGGAAATCACGCTCAAATTGAAAATTGGCGTCAAAAAGAAAGTTTAAAAATCACTTTGC
AAAAAAGACCTGATCTTTTAATTAATAAAAAATTATCCTCAAAAGAAAAAGTGATTTTAGAAGAAATCAAACAAGAATTG
AAAAACAATTCTTAA

Upstream 100 bases:

>100_bases
TCTTGAAAAAAAATTTTGTTTTTCAAAAAGACAATCAAATTAAAAAACCTCAAACTGCAAAATAATTTTTTAACAGAATA
CAAGGAAAGTAATTTTGGTG

Downstream 100 bases:

>100_bases
AATATTTTTAAAAAACCAAAAGGAGAAAGTGTATGTCTCAATTAAAAGGGCAAGGTTTAATCGAATCAGTTACTCAAAAT
CAACTTAAAGAAGTACCTTT

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MIIEIVTIFPLFFKSFCDNSIIKRALNQQKVQIKIHDLRKYSVNKHQQVDDCVYGGGVGMLLSFPPFFDCLQEIKTPQSK
VILLSPQGKIFDQVQANNYASNTPHLIILCGNYEGVDARILKYVDQEISIGDYVLTGGEIAATVVVDAIVRLIPGVIKHE
SAFSDSHQQGLLKFPQYTRPQIYQNQEVPSVLLSGNHAQIENWRQKESLKITLQKRPDLLINKKLSSKEKVILEEIKQEL
KNNS

Sequences:

>Translated_244_residues
MIIEIVTIFPLFFKSFCDNSIIKRALNQQKVQIKIHDLRKYSVNKHQQVDDCVYGGGVGMLLSFPPFFDCLQEIKTPQSK
VILLSPQGKIFDQVQANNYASNTPHLIILCGNYEGVDARILKYVDQEISIGDYVLTGGEIAATVVVDAIVRLIPGVIKHE
SAFSDSHQQGLLKFPQYTRPQIYQNQEVPSVLLSGNHAQIENWRQKESLKITLQKRPDLLINKKLSSKEKVILEEIKQEL
KNNS
>Mature_244_residues
MIIEIVTIFPLFFKSFCDNSIIKRALNQQKVQIKIHDLRKYSVNKHQQVDDCVYGGGVGMLLSFPPFFDCLQEIKTPQSK
VILLSPQGKIFDQVQANNYASNTPHLIILCGNYEGVDARILKYVDQEISIGDYVLTGGEIAATVVVDAIVRLIPGVIKHE
SAFSDSHQQGLLKFPQYTRPQIYQNQEVPSVLLSGNHAQIENWRQKESLKITLQKRPDLLINKKLSSKEKVILEEIKQEL
KNNS

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=244, Percent_Identity=43.4426229508197, Blast_Score=206, Evalue=8e-55,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_PHYAS (B1VAA9)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001799133.1
- ProteinModelPortal:   B1VAA9
- SMR:   B1VAA9
- GeneID:   6798838
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- ProtClustDB:   PRK00026
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27677; Mature: 27677

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIEIVTIFPLFFKSFCDNSIIKRALNQQKVQIKIHDLRKYSVNKHQQVDDCVYGGGVGM
CEEEHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEEEHHHHCCCCCCCHHHHHHCCCHHH
LLSFPPFFDCLQEIKTPQSKVILLSPQGKIFDQVQANNYASNTPHLIILCGNYEGVDARI
HHCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHCCCCCCCCCEEEEEECCCCCHHHHH
LKYVDQEISIGDYVLTGGEIAATVVVDAIVRLIPGVIKHESAFSDSHQQGLLKFPQYTRP
HHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCC
QIYQNQEVPSVLLSGNHAQIENWRQKESLKITLQKRPDLLINKKLSSKEKVILEEIKQEL
CCCCCCCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHH
KNNS
CCCC
>Mature Secondary Structure
MIIEIVTIFPLFFKSFCDNSIIKRALNQQKVQIKIHDLRKYSVNKHQQVDDCVYGGGVGM
CEEEHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEEEHHHHCCCCCCCHHHHHHCCCHHH
LLSFPPFFDCLQEIKTPQSKVILLSPQGKIFDQVQANNYASNTPHLIILCGNYEGVDARI
HHCCCHHHHHHHHHCCCCCCEEEECCCCCHHHHHHCCCCCCCCCEEEEEECCCCCHHHHH
LKYVDQEISIGDYVLTGGEIAATVVVDAIVRLIPGVIKHESAFSDSHQQGLLKFPQYTRP
HHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCC
QIYQNQEVPSVLLSGNHAQIENWRQKESLKITLQKRPDLLINKKLSSKEKVILEEIKQEL
CCCCCCCCCEEEECCCHHHHHHHHCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHHHHH
KNNS
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA