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The map label for this gene is trpS [H]

Identifier: 197294586

GI number: 197294586

Start: 547768

End: 548772

Strand: Direct

Name: trpS [H]

Synonym: PAa_0542

Alternate gene names: 197294586

Gene position: 547768-548772 (Clockwise)

Preceding gene: 197294585

Following gene: 197294587

Centisome position: 62.25

GC content: 27.76

Gene sequence:

>1005_bases
TTGATCCAAAAAAGATTAGTATCAGGTATTAAACCAACGGGGGATTTAACTTTAGGTAATTATATTGGCGTTTTGCAACC
TTTGCTTTTTTTGCAAAAAAATTTAAAAAATTTTGATTTTTACCTTTTTATAGCTGATTTGCATGCCCTTACTTTTTATC
AAGAACCCTCGCAACTAAAAAAACAAATCAAAAAAGTAGCAGCTCTTTATTTGGCTTCAGGATTAGATCCAAAAAAAATA
AACTTATTTATCCAATCAGAAATTATTGAACACTCTTATTTAGGTTATCTCTTGGAATGTAATTCTTATTTTAATGAACT
CCAAAGAATGATTCAATATAAAGAAAAAAGCAAACAAAATAGTAATGGTATGCGAACTTCTTTATTCACTTATCCTACAT
TAATGGCAGCGGATATTTTAATTTACGATGCCAACATTGTTCCAATCGGCCAAGACCAAAAACAACATTTAGAACTAACT
AAAACTCTTGCCACTAGGTTTAATCATTTATACGGTAATACTTTCACAATCCCCGAAGCTTTTTTTAATCCTTTAGGAAC
AAAAATTAAAGGTTTACAAAAACCTTTAAAAAAAATGAGCAAAAGCGAAACTGAAAATGATAAAAATTATATTTTACTTT
TAGATGAACCTGAAATAATTAAGAAAAAAATTATGGCTGCTGTGACAGATTCTGATACGTTCATCAAATACGACCCCGAA
AATAAGCCGAGTATTTCTAACCTTTTAACCATTTATTCTGCCTTAAAAAAAATTAGCATAAGAGAAAGTGAAAAAATATT
TCAATCATCTTCTTATAAATATTTAAAAGATAAAATTTATGAAGCTGTTATAGAATTTATTATTCCTTTACAAAAAAAAT
TTTATCACTTAATTGATAACCCTAAATTAGATGAAATTTTAAATGAGGGGGCCAAAAAAGCTCGTCTGATTGCAGATGCT
AAAATCAAAGAGGTACAAAATAAAATAGGAATTATGAGATTTTAA

Upstream 100 bases:

>100_bases
AGAATCCTTACCCAAAGAATAAAAAAATTAATAATTTTCTATTTGGAAAAACTAACATTCTTTTAAAAATAGATAAAATA
GATAAAAAGGGGATTAAAAA

Downstream 100 bases:

>100_bases
ATTATATTGAAAGTTGGATAAAATTTTAAATGTGTGGACTGTTACATTATTTAGGACAATTTTTTATTTTTTAAAATTCT
TTAAGACTGACTTATTTAAG

Product: Tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase; TrpRS [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MIQKRLVSGIKPTGDLTLGNYIGVLQPLLFLQKNLKNFDFYLFIADLHALTFYQEPSQLKKQIKKVAALYLASGLDPKKI
NLFIQSEIIEHSYLGYLLECNSYFNELQRMIQYKEKSKQNSNGMRTSLFTYPTLMAADILIYDANIVPIGQDQKQHLELT
KTLATRFNHLYGNTFTIPEAFFNPLGTKIKGLQKPLKKMSKSETENDKNYILLLDEPEIIKKKIMAAVTDSDTFIKYDPE
NKPSISNLLTIYSALKKISIRESEKIFQSSSYKYLKDKIYEAVIEFIIPLQKKFYHLIDNPKLDEILNEGAKKARLIADA
KIKEVQNKIGIMRF

Sequences:

>Translated_334_residues
MIQKRLVSGIKPTGDLTLGNYIGVLQPLLFLQKNLKNFDFYLFIADLHALTFYQEPSQLKKQIKKVAALYLASGLDPKKI
NLFIQSEIIEHSYLGYLLECNSYFNELQRMIQYKEKSKQNSNGMRTSLFTYPTLMAADILIYDANIVPIGQDQKQHLELT
KTLATRFNHLYGNTFTIPEAFFNPLGTKIKGLQKPLKKMSKSETENDKNYILLLDEPEIIKKKIMAAVTDSDTFIKYDPE
NKPSISNLLTIYSALKKISIRESEKIFQSSSYKYLKDKIYEAVIEFIIPLQKKFYHLIDNPKLDEILNEGAKKARLIADA
KIKEVQNKIGIMRF
>Mature_334_residues
MIQKRLVSGIKPTGDLTLGNYIGVLQPLLFLQKNLKNFDFYLFIADLHALTFYQEPSQLKKQIKKVAALYLASGLDPKKI
NLFIQSEIIEHSYLGYLLECNSYFNELQRMIQYKEKSKQNSNGMRTSLFTYPTLMAADILIYDANIVPIGQDQKQHLELT
KTLATRFNHLYGNTFTIPEAFFNPLGTKIKGLQKPLKKMSKSETENDKNYILLLDEPEIIKKKIMAAVTDSDTFIKYDPE
NKPSISNLLTIYSALKKISIRESEKIFQSSSYKYLKDKIYEAVIEFIIPLQKKFYHLIDNPKLDEILNEGAKKARLIADA
KIKEVQNKIGIMRF

Specific function: Unknown

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=332, Percent_Identity=35.8433734939759, Blast_Score=218, Evalue=8e-57,
Organism=Homo sapiens, GI41352700, Length=182, Percent_Identity=41.7582417582418, Blast_Score=153, Evalue=2e-37,
Organism=Escherichia coli, GI1789786, Length=335, Percent_Identity=42.6865671641791, Blast_Score=275, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI71982800, Length=329, Percent_Identity=34.9544072948328, Blast_Score=186, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI71982793, Length=329, Percent_Identity=34.9544072948328, Blast_Score=185, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6320474, Length=344, Percent_Identity=37.5, Blast_Score=189, Evalue=7e-49,
Organism=Drosophila melanogaster, GI24666151, Length=332, Percent_Identity=35.8433734939759, Blast_Score=205, Evalue=4e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 38544; Mature: 38544

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQKRLVSGIKPTGDLTLGNYIGVLQPLLFLQKNLKNFDFYLFIADLHALTFYQEPSQLK
CCHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCHHHHH
KQIKKVAALYLASGLDPKKINLFIQSEIIEHSYLGYLLECNSYFNELQRMIQYKEKSKQN
HHHHHHHHHHHHCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SNGMRTSLFTYPTLMAADILIYDANIVPIGQDQKQHLELTKTLATRFNHLYGNTFTIPEA
CCCCCHHHHHHHHHHHHHHEEEECEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEECHHH
FFNPLGTKIKGLQKPLKKMSKSETENDKNYILLLDEPEIIKKKIMAAVTDSDTFIKYDPE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEECCC
NKPSISNLLTIYSALKKISIRESEKIFQSSSYKYLKDKIYEAVIEFIIPLQKKFYHLIDN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PKLDEILNEGAKKARLIADAKIKEVQNKIGIMRF
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIQKRLVSGIKPTGDLTLGNYIGVLQPLLFLQKNLKNFDFYLFIADLHALTFYQEPSQLK
CCHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCHHHHH
KQIKKVAALYLASGLDPKKINLFIQSEIIEHSYLGYLLECNSYFNELQRMIQYKEKSKQN
HHHHHHHHHHHHCCCCCHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SNGMRTSLFTYPTLMAADILIYDANIVPIGQDQKQHLELTKTLATRFNHLYGNTFTIPEA
CCCCCHHHHHHHHHHHHHHEEEECEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEECHHH
FFNPLGTKIKGLQKPLKKMSKSETENDKNYILLLDEPEIIKKKIMAAVTDSDTFIKYDPE
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEECCC
NKPSISNLLTIYSALKKISIRESEKIFQSSSYKYLKDKIYEAVIEFIIPLQKKFYHLIDN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PKLDEILNEGAKKARLIADAKIKEVQNKIGIMRF
CCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3149612; 9384377; 2494170 [H]