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The map label for this gene is gltX

Identifier: 197294543

GI number: 197294543

Start: 493337

End: 494683

Strand: Direct

Name: gltX

Synonym: PAa_0498

Alternate gene names: 197294543

Gene position: 493337-494683 (Clockwise)

Preceding gene: 197294542

Following gene: 197294544

Centisome position: 56.06

GC content: 28.36

Gene sequence:

>1347_bases
ATGAATAAAATAAGAGTTCGTTATGCTCCAAGTCCAACTGGTTTTTTACATATAGGCAATGCCCGAACTGCTTTATTCAA
TTATTTATTCGCTAAACATCATCAAGGAGATTTTATTTTAAGGATTGAAGATACTGATCTTTTGCGAAATGTTGCAGATG
GAGAGGCTTCGCAGTTAAAAAATTTGCGTTGGCTTGGGATTGATTGGAAGGAAGGACCCGATATAAATGGTCCTTTCGGT
CCTTATCGTCAATCTGAAAGATTAACAATTTATCAAAAATATGCTTTCGAGTTGCTTGAAAAAAACCTTGCTTATCGCGA
TTTTGAAAAAGATAAAAAAAACTTTGCTATTAGATTCAAAGTTCCTCCAAACCAAACTTTTACCTTTTGTGATTTAATTA
GGGGTAAATTGACTTTTTTGAGCAAGGAAATTGAAGATTGGGTCATTATTAAATCAAACGGCTATCCTTCTTATAATTTT
GCTGCTTCCATTGATGACCATTTAATGCAAATTTCTCATATTTTTCGTGGCGAGGAACACATCACAAACACTCCTAAACA
AATAATGATTTATCAATCTTTTAATTGGTCAATTCCTAAATTCGCTCATATGACTTTAATTTTAAACCAAGAAAGAAAGA
AACTGAGCAAAAGAGATGTTAATGTTTGCCAGTTTATCCAAGATTATGCTGATTTGGGTTATCTTCCTCAATCGTTATTT
AATTTTTTATCTCTTTTAGGTTTTTCTCCTTCAAGTTGTAAAGAAATATTGACCCCTCAAGAAATTATTTCTTTATTTGA
TGTTAATCGTTTAAACAAATCTCCAGCTATTTTTGATAAAACAAAATTAGATTTTTTTAATAATCATTACCTTCGCAAAA
CACCTATTGATGATATTGTTTCATTTATAAAAACAAAGTTAGATTTTTTTGAAGTTTTGCCTATAAACAATCAAAAATGG
TTAACTAAATTTATTCTTTTATTTCAAGAAAGAATAAATTATATAAAACAATTAAAAGATTTATATCATCATTTTTTTGA
CAAAAATACTTCTTTATCCGAGGAAGTGGAGCAATTTTTAAAAAAACATGATTGCGCCTTATCTGTTTTAACTCTTTTTT
ATCATAAATTAAATTTAGTTGTTTTTGAAAAAGAAGCAATTAACCCTATTATTGCTGAAATAGCTCAAAATATGAAAATT
AATAAAAAAAATCTTTTCGTAATCTTGAGAATTGGGGCAACTCACAAAATGCAAGGTCCTAGTTTGGCGCTATTTTTAGA
ACTGTTAGGAAAAAAACAAGTACTTAATAATTTATCTAAAATTGTAGAATTATTAAAAAACCAATAA

Upstream 100 bases:

>100_bases
CCGAGCTCCGCAATTCTCTAAACGTTAATAAAAAGAACTTTTTAATAAAGTTCTTTTTTTATCAAATAAACACTATAAAA
TAAAAAAAGAGAAAGAACAT

Downstream 100 bases:

>100_bases
TCTTGGTTATTTTGAATTTTTTTTACTTTTAAATAATGACAATTTTTTGAATAAAGCCAAAAACCACAAAAAATAATTAT
TTTTTTAAATATATAATTTT

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MNKIRVRYAPSPTGFLHIGNARTALFNYLFAKHHQGDFILRIEDTDLLRNVADGEASQLKNLRWLGIDWKEGPDINGPFG
PYRQSERLTIYQKYAFELLEKNLAYRDFEKDKKNFAIRFKVPPNQTFTFCDLIRGKLTFLSKEIEDWVIIKSNGYPSYNF
AASIDDHLMQISHIFRGEEHITNTPKQIMIYQSFNWSIPKFAHMTLILNQERKKLSKRDVNVCQFIQDYADLGYLPQSLF
NFLSLLGFSPSSCKEILTPQEIISLFDVNRLNKSPAIFDKTKLDFFNNHYLRKTPIDDIVSFIKTKLDFFEVLPINNQKW
LTKFILLFQERINYIKQLKDLYHHFFDKNTSLSEEVEQFLKKHDCALSVLTLFYHKLNLVVFEKEAINPIIAEIAQNMKI
NKKNLFVILRIGATHKMQGPSLALFLELLGKKQVLNNLSKIVELLKNQ

Sequences:

>Translated_448_residues
MNKIRVRYAPSPTGFLHIGNARTALFNYLFAKHHQGDFILRIEDTDLLRNVADGEASQLKNLRWLGIDWKEGPDINGPFG
PYRQSERLTIYQKYAFELLEKNLAYRDFEKDKKNFAIRFKVPPNQTFTFCDLIRGKLTFLSKEIEDWVIIKSNGYPSYNF
AASIDDHLMQISHIFRGEEHITNTPKQIMIYQSFNWSIPKFAHMTLILNQERKKLSKRDVNVCQFIQDYADLGYLPQSLF
NFLSLLGFSPSSCKEILTPQEIISLFDVNRLNKSPAIFDKTKLDFFNNHYLRKTPIDDIVSFIKTKLDFFEVLPINNQKW
LTKFILLFQERINYIKQLKDLYHHFFDKNTSLSEEVEQFLKKHDCALSVLTLFYHKLNLVVFEKEAINPIIAEIAQNMKI
NKKNLFVILRIGATHKMQGPSLALFLELLGKKQVLNNLSKIVELLKNQ
>Mature_448_residues
MNKIRVRYAPSPTGFLHIGNARTALFNYLFAKHHQGDFILRIEDTDLLRNVADGEASQLKNLRWLGIDWKEGPDINGPFG
PYRQSERLTIYQKYAFELLEKNLAYRDFEKDKKNFAIRFKVPPNQTFTFCDLIRGKLTFLSKEIEDWVIIKSNGYPSYNF
AASIDDHLMQISHIFRGEEHITNTPKQIMIYQSFNWSIPKFAHMTLILNQERKKLSKRDVNVCQFIQDYADLGYLPQSLF
NFLSLLGFSPSSCKEILTPQEIISLFDVNRLNKSPAIFDKTKLDFFNNHYLRKTPIDDIVSFIKTKLDFFEVLPINNQKW
LTKFILLFQERINYIKQLKDLYHHFFDKNTSLSEEVEQFLKKHDCALSVLTLFYHKLNLVVFEKEAINPIIAEIAQNMKI
NKKNLFVILRIGATHKMQGPSLALFLELLGKKQVLNNLSKIVELLKNQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=495, Percent_Identity=31.5151515151515, Blast_Score=228, Evalue=1e-59,
Organism=Escherichia coli, GI1788743, Length=481, Percent_Identity=30.3534303534304, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI226510925, Length=234, Percent_Identity=32.0512820512821, Blast_Score=112, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI71993394, Length=485, Percent_Identity=31.5463917525773, Blast_Score=221, Evalue=9e-58,
Organism=Saccharomyces cerevisiae, GI6324540, Length=510, Percent_Identity=30.7843137254902, Blast_Score=221, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24665321, Length=504, Percent_Identity=30.1587301587302, Blast_Score=219, Evalue=4e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_PHYAS (B1VA59)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001799084.1
- ProteinModelPortal:   B1VA59
- SMR:   B1VA59
- GeneID:   6799630
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG628189
- OMA:   NGYPSYN
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52518; Mature: 52518

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 217-217

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIRVRYAPSPTGFLHIGNARTALFNYLFAKHHQGDFILRIEDTDLLRNVADGEASQLK
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCHHHHC
NLRWLGIDWKEGPDINGPFGPYRQSERLTIYQKYAFELLEKNLAYRDFEKDKKNFAIRFK
CCEEEECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
VPPNQTFTFCDLIRGKLTFLSKEIEDWVIIKSNGYPSYNFAASIDDHLMQISHIFRGEEH
ECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCHHHHHHHHHHHHCCCHH
ITNTPKQIMIYQSFNWSIPKFAHMTLILNQERKKLSKRDVNVCQFIQDYADLGYLPQSLF
HCCCCCCEEEEECCCCCCCHHHHHHHEECHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHH
NFLSLLGFSPSSCKEILTPQEIISLFDVNRLNKSPAIFDKTKLDFFNNHYLRKTPIDDIV
HHHHHHCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCHHHHH
SFIKTKLDFFEVLPINNQKWLTKFILLFQERINYIKQLKDLYHHFFDKNTSLSEEVEQFL
HHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KKHDCALSVLTLFYHKLNLVVFEKEAINPIIAEIAQNMKINKKNLFVILRIGATHKMQGP
HHHHHHHHHHHHHHHHHHEEEEEHHHCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCC
SLALFLELLGKKQVLNNLSKIVELLKNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKIRVRYAPSPTGFLHIGNARTALFNYLFAKHHQGDFILRIEDTDLLRNVADGEASQLK
CCCEEEEECCCCCCEEEECCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHCCCCHHHHC
NLRWLGIDWKEGPDINGPFGPYRQSERLTIYQKYAFELLEKNLAYRDFEKDKKNFAIRFK
CCEEEECCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
VPPNQTFTFCDLIRGKLTFLSKEIEDWVIIKSNGYPSYNFAASIDDHLMQISHIFRGEEH
ECCCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEECCHHHHHHHHHHHHCCCHH
ITNTPKQIMIYQSFNWSIPKFAHMTLILNQERKKLSKRDVNVCQFIQDYADLGYLPQSLF
HCCCCCCEEEEECCCCCCCHHHHHHHEECHHHHHHHHCCHHHHHHHHHHHHCCCCHHHHH
NFLSLLGFSPSSCKEILTPQEIISLFDVNRLNKSPAIFDKTKLDFFNNHYLRKTPIDDIV
HHHHHHCCCHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCCCCCCHHHHH
SFIKTKLDFFEVLPINNQKWLTKFILLFQERINYIKQLKDLYHHFFDKNTSLSEEVEQFL
HHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KKHDCALSVLTLFYHKLNLVVFEKEAINPIIAEIAQNMKINKKNLFVILRIGATHKMQGP
HHHHHHHHHHHHHHHHHHEEEEEHHHCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCC
SLALFLELLGKKQVLNNLSKIVELLKNQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA