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The map label for this gene is ksgA

Identifier: 197294383

GI number: 197294383

Start: 301582

End: 302436

Strand: Reverse

Name: ksgA

Synonym: PAa_0272

Alternate gene names: 197294383

Gene position: 302436-301582 (Counterclockwise)

Preceding gene: 197294384

Following gene: 197294382

Centisome position: 34.37

GC content: 24.56

Gene sequence:

>855_bases
ATGCAACATCAACCCAAAAAAAAATACGGTCAAAATTTTTTAAAAGATGTTAATTTATTAAAAAAAATAGTTTCTAAAGC
TAATTTAAAAGGGAAAAACGTAATTGAAATTGGTCCCGGAAAAGGGTCTCTAACTAATCTTATTACAAAGGAAGCCAATT
TACTTTTAGCTTATGAAATAGACCCCACTTTAAAACCTTTTTTAGTTTTTGATACAACTAAGATCAAAATTATTTATGAT
GATTTTTTAAAAAGAGATTTAGTTAAAGATTTTGATAATTATTTTTCTATCAATTGTCAATTGTCTTTGATTAGTAATTT
GCCTTATTATATTACCACCACCATTCTTTTTAAAATCATTCAAACCCCGCAAATCGTTGATGCAACTTTGATGATGCAAA
AAGAAGTAGGAATGCGCTTAATGGCTCAACCTAATAGCAAAAACTATAATGCTTTATCAGTTATAACACAATATTTTTTT
AATATCGAAAAAATTCAAGAAGTTAAAAGTCATATGTTTTTCCCCCAACCTAAAGTGGATAGCGTTGTTTTAAAATTAAG
CAAACATAAAAGCGATTTTAATACCTTTTCTACTTCATCTCAAAAAAACTTTATTACGTTTGTGAAAGCTGCTTTTAAAC
AAAAAAGAAAAACTTTATTAAATAATTTATCGAGTGTATTTTTATTACCTAAAACAGAAATTATTCTTTTTTTTGAGCAA
AACAAACTTTTGACAAAAATTAGAGCTGAAGAAATAACATTAAAAGAATTTCAAAAAATAAGTATTCAATGGTTTTTATT
CCAAAATAAAACAAAAGAGAGTCAATTAAATAATTATATATTAAAAAAAGTTTAA

Upstream 100 bases:

>100_bases
TCTATTATTAAAAATTTGATTAGAAACGGTGTAAAAACAGATAATAAATTTTTTTACCGTCCTTTTGAAAAGAAATAATA
AGAATGAGGCAGAGGCCTAA

Downstream 100 bases:

>100_bases
TAATTATTTATATTTTATGTTAAAATGACAAGTAGTTTAAAGTATTTTATAGCTATTAAATTATGAAAACAATTAAGAAA
ATTTCAGAAGATAAATCAAA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MQHQPKKKYGQNFLKDVNLLKKIVSKANLKGKNVIEIGPGKGSLTNLITKEANLLLAYEIDPTLKPFLVFDTTKIKIIYD
DFLKRDLVKDFDNYFSINCQLSLISNLPYYITTTILFKIIQTPQIVDATLMMQKEVGMRLMAQPNSKNYNALSVITQYFF
NIEKIQEVKSHMFFPQPKVDSVVLKLSKHKSDFNTFSTSSQKNFITFVKAAFKQKRKTLLNNLSSVFLLPKTEIILFFEQ
NKLLTKIRAEEITLKEFQKISIQWFLFQNKTKESQLNNYILKKV

Sequences:

>Translated_284_residues
MQHQPKKKYGQNFLKDVNLLKKIVSKANLKGKNVIEIGPGKGSLTNLITKEANLLLAYEIDPTLKPFLVFDTTKIKIIYD
DFLKRDLVKDFDNYFSINCQLSLISNLPYYITTTILFKIIQTPQIVDATLMMQKEVGMRLMAQPNSKNYNALSVITQYFF
NIEKIQEVKSHMFFPQPKVDSVVLKLSKHKSDFNTFSTSSQKNFITFVKAAFKQKRKTLLNNLSSVFLLPKTEIILFFEQ
NKLLTKIRAEEITLKEFQKISIQWFLFQNKTKESQLNNYILKKV
>Mature_284_residues
MQHQPKKKYGQNFLKDVNLLKKIVSKANLKGKNVIEIGPGKGSLTNLITKEANLLLAYEIDPTLKPFLVFDTTKIKIIYD
DFLKRDLVKDFDNYFSINCQLSLISNLPYYITTTILFKIIQTPQIVDATLMMQKEVGMRLMAQPNSKNYNALSVITQYFF
NIEKIQEVKSHMFFPQPKVDSVVLKLSKHKSDFNTFSTSSQKNFITFVKAAFKQKRKTLLNNLSSVFLLPKTEIILFFEQ
NKLLTKIRAEEITLKEFQKISIQWFLFQNKTKESQLNNYILKKV

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=218, Percent_Identity=31.651376146789, Blast_Score=95, Evalue=7e-20,
Organism=Homo sapiens, GI156415992, Length=204, Percent_Identity=31.8627450980392, Blast_Score=90, Evalue=3e-18,
Organism=Escherichia coli, GI1786236, Length=267, Percent_Identity=33.3333333333333, Blast_Score=127, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI25146882, Length=222, Percent_Identity=31.981981981982, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI25141369, Length=211, Percent_Identity=27.0142180094787, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6324989, Length=199, Percent_Identity=34.1708542713568, Blast_Score=100, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21358017, Length=255, Percent_Identity=31.7647058823529, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21357273, Length=206, Percent_Identity=29.6116504854369, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_PHYAS (B1V9I5)

Other databases:

- EMBL:   AM422018
- RefSeq:   YP_001798924.1
- ProteinModelPortal:   B1V9I5
- SMR:   B1V9I5
- GeneID:   6799104
- GenomeReviews:   AM422018_GR
- HOGENOM:   HBG319664
- OMA:   RAENLTP
- ProtClustDB:   PRK00274
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 33072; Mature: 33072

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 12-12 BINDING 14-14 BINDING 38-38 BINDING 59-59 BINDING 81-81 BINDING 106-106

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHQPKKKYGQNFLKDVNLLKKIVSKANLKGKNVIEIGPGKGSLTNLITKEANLLLAYEI
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCEEEEEEC
DPTLKPFLVFDTTKIKIIYDDFLKRDLVKDFDNYFSINCQLSLISNLPYYITTTILFKII
CCCCCCEEEEECEEEEEEHHHHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHH
QTPQIVDATLMMQKEVGMRLMAQPNSKNYNALSVITQYFFNIEKIQEVKSHMFFPQPKVD
CCCCHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
SVVLKLSKHKSDFNTFSTSSQKNFITFVKAAFKQKRKTLLNNLSSVFLLPKTEIILFFEQ
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEEECCCEEEEEEEC
NKLLTKIRAEEITLKEFQKISIQWFLFQNKTKESQLNNYILKKV
CHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure
MQHQPKKKYGQNFLKDVNLLKKIVSKANLKGKNVIEIGPGKGSLTNLITKEANLLLAYEI
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHCCCCEEEEEEC
DPTLKPFLVFDTTKIKIIYDDFLKRDLVKDFDNYFSINCQLSLISNLPYYITTTILFKII
CCCCCCEEEEECEEEEEEHHHHHHHHHHHHHHHHEEEEEEEEEECCCCHHHHHHHHHHHH
QTPQIVDATLMMQKEVGMRLMAQPNSKNYNALSVITQYFFNIEKIQEVKSHMFFPQPKVD
CCCCHHHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
SVVLKLSKHKSDFNTFSTSSQKNFITFVKAAFKQKRKTLLNNLSSVFLLPKTEIILFFEQ
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHEEEECCCEEEEEEEC
NKLLTKIRAEEITLKEFQKISIQWFLFQNKTKESQLNNYILKKV
CHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA