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The map label for this gene is truA [H]

Identifier: 197294307

GI number: 197294307

Start: 203889

End: 204626

Strand: Reverse

Name: truA [H]

Synonym: PAa_0170

Alternate gene names: 197294307

Gene position: 204626-203889 (Counterclockwise)

Preceding gene: 197294308

Following gene: 197294306

Centisome position: 23.25

GC content: 27.37

Gene sequence:

>738_bases
ATGTTTTTGGAAAAGTATTTTTATAAATTAATTTTAAGTTATGATGGCACTTGTTATTGCGGATACCAAAAACAACCCCA
AACAAAAACGATTCAACAAACATTAGAAAATGCTTTAAAATCGATGACTAAACGTCAAATTAATACTTTTGCAGCTAGCA
GAACTGATAAAGGTGTACATGCTCAAGAACAAACAATTCATTTTCAAACTGCTTTTGCGATTGAACTAAAACGTTTTAAA
AACACTCTTAATCGTATTTTACCGCCTGATATTAGAATTAAAAAAATAGAAAAAGCAACAGCTGATTTTCATGCTCGTTA
TTGGGCAAAATCCAAAATTTACCATTATATTTTTGCCAAAAAACCTTTAAATGCTTTTAATTATCATTTTCAAGTTTTTG
TTGCTAACCTTGATTTTGCAAAAATAAAATCCGCTATGAATTTGATTATTGGAAAATATAATTTTGCCTCTTTCACTAAC
GACAAAAAAGAAAAAAATTTTAATAAAACTGTTTTAAATGCTTTTGTGAAAGAAACTTCCCAAAATTATATTTTAATTTT
TCATGCAGATGGTTTTCTTAAGTATATGGTTCGATTTTTGGTGGGGGGGTTGATTGAAATCGGGAAAAATAAAATGTCTT
TAGAACTTTTTAAAGCTATGTTAACCAACACAACTAATCAAAAAGTATCCCTTTTAGCTCCAGCGAAAGGTTTATTTTTA
AAAAAAATTTTTTATTAA

Upstream 100 bases:

>100_bases
TAACTACCATAAGTTTTTACATCTGCTGAAAAAGTGGATTGTTTTTAAAAAAAATTTAATGATTGTTATTTTTTTATGCT
TTATCATTAATTTTTAGGAG

Downstream 100 bases:

>100_bases
ATTATAATTTGTTTTTTCTATTTTTCATTTAACCCCAAAATTAAAATTGGAGAGTTAACAAAATAAAAAAAGGAGAATTA
AGATTATTATGTTTATTTCT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MFLEKYFYKLILSYDGTCYCGYQKQPQTKTIQQTLENALKSMTKRQINTFAASRTDKGVHAQEQTIHFQTAFAIELKRFK
NTLNRILPPDIRIKKIEKATADFHARYWAKSKIYHYIFAKKPLNAFNYHFQVFVANLDFAKIKSAMNLIIGKYNFASFTN
DKKEKNFNKTVLNAFVKETSQNYILIFHADGFLKYMVRFLVGGLIEIGKNKMSLELFKAMLTNTTNQKVSLLAPAKGLFL
KKIFY

Sequences:

>Translated_245_residues
MFLEKYFYKLILSYDGTCYCGYQKQPQTKTIQQTLENALKSMTKRQINTFAASRTDKGVHAQEQTIHFQTAFAIELKRFK
NTLNRILPPDIRIKKIEKATADFHARYWAKSKIYHYIFAKKPLNAFNYHFQVFVANLDFAKIKSAMNLIIGKYNFASFTN
DKKEKNFNKTVLNAFVKETSQNYILIFHADGFLKYMVRFLVGGLIEIGKNKMSLELFKAMLTNTTNQKVSLLAPAKGLFL
KKIFY
>Mature_245_residues
MFLEKYFYKLILSYDGTCYCGYQKQPQTKTIQQTLENALKSMTKRQINTFAASRTDKGVHAQEQTIHFQTAFAIELKRFK
NTLNRILPPDIRIKKIEKATADFHARYWAKSKIYHYIFAKKPLNAFNYHFQVFVANLDFAKIKSAMNLIIGKYNFASFTN
DKKEKNFNKTVLNAFVKETSQNYILIFHADGFLKYMVRFLVGGLIEIGKNKMSLELFKAMLTNTTNQKVSLLAPAKGLFL
KKIFY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Escherichia coli, GI1788657, Length=243, Percent_Identity=28.3950617283951, Blast_Score=116, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17565126, Length=268, Percent_Identity=25, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007752, Length=307, Percent_Identity=28.3387622149837, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281362146, Length=262, Percent_Identity=26.7175572519084, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24648455, Length=262, Percent_Identity=26.7175572519084, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28560; Mature: 28560

Theoretical pI: Translated: 10.50; Mature: 10.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLEKYFYKLILSYDGTCYCGYQKQPQTKTIQQTLENALKSMTKRQINTFAASRTDKGVH
CCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AQEQTIHFQTAFAIELKRFKNTLNRILPPDIRIKKIEKATADFHARYWAKSKIYHYIFAK
CHHHEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
KPLNAFNYHFQVFVANLDFAKIKSAMNLIIGKYNFASFTNDKKEKNFNKTVLNAFVKETS
CCCCHHCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHCC
QNYILIFHADGFLKYMVRFLVGGLIEIGKNKMSLELFKAMLTNTTNQKVSLLAPAKGLFL
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHH
KKIFY
HHHCC
>Mature Secondary Structure
MFLEKYFYKLILSYDGTCYCGYQKQPQTKTIQQTLENALKSMTKRQINTFAASRTDKGVH
CCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
AQEQTIHFQTAFAIELKRFKNTLNRILPPDIRIKKIEKATADFHARYWAKSKIYHYIFAK
CHHHEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEEC
KPLNAFNYHFQVFVANLDFAKIKSAMNLIIGKYNFASFTNDKKEKNFNKTVLNAFVKETS
CCCCHHCEEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCHHHHHHHHHHHHCC
QNYILIFHADGFLKYMVRFLVGGLIEIGKNKMSLELFKAMLTNTTNQKVSLLAPAKGLFL
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCEEEEECCCCHHHH
KKIFY
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA